교통사고 후 발생한 경항통 환자에 대한 황련해독탕약침과 분리정제봉약침의 치료 효과 비교연구: 무작위 배정

이옥진 ( Yu Chen Lee ),남대진 ( Dae Jin Nam ),허건 ( Gun Huh ),이재은 ( Jae Eun Lee ),김빛나라 ( Bin Na Ra Kim ),송은모 ( Eun Mo Song ),이은정 ( Eun Jung Lee ),이정민 ( Jung Min Lee ),오민석 ( Min Seok Oh ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.4

ObjectivesThe purpose of this study is to compare the effects of HWANGRY-UNHAEDOKTANG Pharmacopuncture and Essential Bee Venom pharmacopuncture on cervical pain caused by traffic accident.MethodsThe clincal study was conducted to traffic accident patients who had admitted to Dae-Jeon University Cheonan Oriental Hospital from May 17, 2014 to September 2, 2014. The patients were simple randomization and divided into two groups. In one group, patients were treated with the oriental medicine and HWANGRYUNHAEDOKTANGPharmacopuncture, While in the other group, patients were treated with the oriental medi-cine therapy and Essential Bee Venom pharmacopuncture therapy. visual analogue scale (VAS), pain threshold and neck disablity index (NDI) were used To estimate the efficacy of Pharmacopuncture treatment.Results1) HWANGRYUNHAEDOKTANG Pharmacopuncture Group showed significant decrease of VAS and NDI after 3rd times treatment than before treatment (p<0.001). Essential Bee Venom Pharmacopuncture showed significant decrease of VAS, NDI and improve ment of pain threshold after 3rd times treatment than before treatment (p<0.01, p<0.001). 2) There was no significant difference between HWANGRYUNHAEDOKTANGPharmacopuncture group and Essential Bee Venom Pharmacopuncture group in VAS, pain threshold and NDI. ConclusionsWe found out that HWANGRYUNHAEDOKTANG Pharmacopuncture and Essential Bee Venom Pharmacopuncture treatment are considered to be effective and useful in cervical pain caused by traffic accident. (J Korean Med Rehab 2014;24(4): 145-153)

족저압 측정에 의한 편마비 환자의 입각기의 분석

홍대종,박시복,이상건,이강목 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.5

Water-in-Carbon Dioxide 마이크로에멀젼에서 나노 크기의 이산화티탄 합성 및 p-니트로페놀의 광분해에 대한 연구

이만식,이근대,홍성수 한국화학공학회 2002 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.40 No.4

CO_2에 용해 능력이 우수한 PFPE-NH_4(ammonium carboxylate perfluoro polyether) 및 PDMAEMA(poly(2-(dimethylamino)-ethyl methacrylate))-b-PFOMA(polu(1H, 1H, 2H, 2H-perfluoroocthyl methacrylate)) 계면활성제를 사용하여 W/C 마이크로에멀젼에 의해 나노 크기의 TiO_2를 제조하였다. TGA-DTA, FT-IR, XRD, TEM 등을 이용하여 나노 입자 제조시 W_0(H_2O/surfactant molar ratio)비에 따른 입자의 크기 및 결정성 등 물리적 성질을 조사하였다. 또한 제조된 TiO_2 나노 입자의 광촉매적 특성을 알아보기 위해 회분식 반응장치를 이용하여 p-니트로페놀의 광활성을 조사하였다. 제조된 TiO_2 나노 입자는 소성온도 250-450℃ 범위에서 유기물질과 수산화물이 분해 되어 450℃ 이상 비결정 구조에서 anatase 구조로 상 전이 되었다. W/C 마이크로에멀젼에서 제조된 나노 입자의 결정성 및 결정크기는 W_0 비가 증가할수록 증가하였다. p-니트로페놀의 광분해반응에서 반응성은 결정크기에 영향을 받았으며, 결정의 크기가 작을수록 반응성이 증가하였다. Titania nanoparticles were prepared by controlled hydrolysis of titanium tetraisopropoxide(TTIP) in PFPE-NH_4(ammonium carboxylate perfluoropolyether) and PDMAEMA(poly(2-(dimethylamino)ethyl methacrylate))-b-PFOMA(poly(1H, 1H, 2H, 2H-perfluoroocthyl methacrylate))/Water-in-CarbonDioxide Microemulsions. The physical properties, such as crystallite size and crystallinity according to W_0 ratio have been investigated by TGA-DTA, FT-IR, XRD and TEM. In addition, the photocatalytic degradation of p-nitrophenol has been studied by using batch reactor in the presence of UV light in order to compare the photocatalytic activity of prepared nanosized titania. The residual organic compound and hydroxyl group were completely removed in calcination temperature from the 250-450℃ and the amorphous phase changed into anatase structure above 450℃. The crystallinity and crystallite size of nanoparticles produced in water-in-carbon dioxide microemulsions increased with an increase of the W_o ratio. In the photocatalytic degradation of p-nitrophenol, the photocatalytic activity is mainly determined by the crystallite size of titania and the reaction rate increased with an decrease of crystallite size.

Midazolam과 Diazepam의 Ketamine에 의한 심혈관계 및 안압 상승 억제효과

이정은,전대근 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

The effect of midazolam and diazepam on increased blood pressure, heart rate and intraocular pressure by ketamine were evaluated in thirty patients. All patients were devided into three groups as follow; Group 1 (n=10): Ketamine 2mg/kg was administered intravenously before endotracheal intubation(control). Group 2 (n=10): Midazolam 0.15mg/kg was administered intravenously three munutes before ketamine administration. Group 3 (n=10): Diazepam 0.3mg/kg was administered intravenously three minutes before ketamine administration. The changes of systolic and diastolic blood pressure, heart rate and intraocular pressure were measured following drug administration and endotracheal intubation in all groups. Succinylcholine 1mg/kg was administered intravenously for endotracheal intubation. The results were as follows; 1) Increased systolic and diastolic blood pressure, heart rate and intraocular pressure due to ketamine(2mg/kg) i.v. administration were prevented with midazolam(0.15mg/kg) or diazepam(0.3mg/kg) i.v. pretreatement effectively. 2) Anterograde amnesia effect was good enough in midazolam and diazepam iv. pretreatment groups. 3) There was no pain with midazolam i.v. administration and showed good tranquillizing effect. From the above result, midazolam i.v. pretreatment is recommended to offset of increased cardiovascular effects and increased intraocular pressure due to ketamine administration.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

자기공명 분석법을 이용한 백서 피판의 대사에 대한 연구

이윤,이대근,임태환,윤근철,고경석,이춘신,정복성 대한성형외과학회 1992 Archives of Plastic Surgery Vol.19 No.3

Innumerable techniques have been used to measure, index, or predict the viability of skin flaps after elevation. However, these are largely indirect and don't accurately reflect the events that occur at kthe cellular level. A method was sought that gives direct, reproducible, and accurate data about physiological and biochemical changes that occur during flap elevation. Because of its ability to monitor changes in the levels of high energy phosphorus metabolites(ATP, or adenosine triphosphate, PC or phosphocreatine, Pi or inorganic phosphate), 31-P magnetic resonance spectroscopy (MRS) holds promise of providing direct assessment of the metabolic status and biochemical changes that occur during skin flap elevation. MRS monitoring was performed on three portions of raised random pattern skin flaps of 5 rats. The spectrometer used for 31-P nuclear magnetic resonance analysis was a Bruker Biospec(4.7 Tesla) system, and a modified surface coil constructed of two layers of copper coil was used. The results have validated the ability of 31-P MRS to : 1. define the regional flxes in the levels of ATP, PCr, and Pi in skin flaps. 2. demonstrate that, of these, PCr levels represent the most sensitive indicator of flap well being. 3. measure intracellular pH through the chemical shift of the Pi resonance. 4. establish predictive correlations between profile of normal, compromised, and failing skin flaps and have thus prepared the way for noninvasive in vivo topical magnetic resonance investigations of flaps.

생쥐 안구에서 산화적 손상에 의한 superoxide dismutases와 metallothionein의 나이에 따른 유도의 차이

이홍영,이태범,안춘산,변익건,최석민,권대승,최철희 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

The basal levels and inducibility of superoxide dismutases (SODs; Mn-SOD and Cu/Zn-SOD) and metallothionein (MT) was investigated in the eyes of mice with age. Oxidative stress was induced by paraquat, an intracellular superoxide generator, in 1, 4, 8, and 12 months of age and then mRl\As of SODs and MT in the eyes were determined by RT-PCR assay. The basal level of Mn-SOD mRNA increased from 1 month to 8 months but decreased thereafter. Mn-SOD mRNA was induced by paraquat until 4 month but not after 8 months. On the other hand, the basal level of Cu/Zn-SOD mRNA decreased with age. Cu/Zn-SOD mRNA was not induced by paraquat until 4 months. The basal level of MT mRNA declined with age whereas its inducibility by paraquat was intact at all ages. Taken together, the results indicate that SODs and MT are differentially expressed and induced according to the age. It could be also implicated that failure in induction of Mn-SOD with age may be one of causative factors in the aging process whereas induction of Cu/Zn-SOD and MT may be one of important factors in defense against oxidative stress in the eyes. 서론: 최근 노화는 반응성 산소종에 의한 산화적 손상의 축적결과라는 설이 각광을 받고 있다. 노화과정에서 노화와 관련된 질병을 비교적 잘 나타내는 안구를 선정하고, 정상생쥐의 안구에서의 superoxide dismutases (SODs) 와 metallothionein (MT) 의 함량 뿐 아니라 산화성 스트레스를 유발하는 paraquat에 의한 SODs와 MT의 유도능과 나이와의 상호관계를 조사하여 이들의 노화과정에서의 역할을 규명하고자 하였다. 재료 및 방법 : 본 연구에서 1, 4, 8, 12개월된 정상생쥐의 안구에서 반응성 산소종 생성의 초기에 중요한 방어기전인 SODs mRNA와 hydroxy radical (OH·)에 의한 산화적 손상의 마지막 방어기전인 MT mRNA의 함량과 산화성 스트레스를 유발하는 paraquat에 의한 유도능을 역전사 중합효소 연쇄반응 (RT-PCR) 으로 조사하였다. 결과: 정상생쥐의 안구의 노화과정에서 Mn-SOD와 Cu/Zn-SOD, 그리고 MT mRNA의 항정상태 함량을 조사한 결과, Mn-SOD mRNA는 8개월까지는 증가하다가 12개월에서 감소하였으나, Cu/Zn-SOD mRNA는 나이와 함께 감소하였다. 그러나 MT mRNA 는 8개월까지는 감소하다가 12개월에서 약간 증가하였다. 산화성 스트레스를 유발하는 parraquat에 의하여 Mn-SOD mRNA는 1개월과 4개월에서만 유도되었고, Cu/Zn-SOD mRNA는 8개월과 12개월에서만 유도되었으며, MT mRNA는 관찰한 전 기간에서 유도되었다. 결론: 이상의 결과를 종합하면 SODs와 MT는 안구에서 나이에 따라 서로 다르게 발현되고 유도되는 것으로 생각되며, 노화과정에서 Mn-SOD유도의 이상이 노화의 원인이 되는 인자로 작용하고, 반면에 Cu/Zn-SOD와 MT의 정상적인 유도능이 산화적 스트레스에 대하여 방어역할을 하는 것으로 생각된다. 이와 같이 연령이 증가함에 따라 Mn-SOD, Cu-Zn-SOD, 및 MT의 발현과 유도능의 차이가 있게 되고, 그 결과 반응성 산소종에 의한 산화적 손상의 정도의 차이로 노화의 속도가 조절되지 않나 생각된다.

Semi-additive 방법을 이용한 연성회로기판 제작에 관한 연구

李宰鎬,金大根 弘益大學校 科學技術硏究所 2005 科學技術硏究論文集 Vol.16 No.-

COF(Chip on Film) which is used of various electronic products, is tending increase its demand due to light and thin products. At present, the manufacturing process of COF usually is used of subtractive method which deposited copper on polyimide film. However the width of line is smaller, the subtractive method has a lateral etching problems. In semi-additive method, copper line is fabricated by lithographic technique followed by electroplating method. Fine line patterns 10~40㎛ were used for this study. Two different types of thick photoresist were used. Fine copper lines were fabricated by electroplating method. The high residual stress caused the crack development in fine lines and then the low residual stress bath was used sacrificing the electroplating rate.

W/O 마이크로에멀젼에 의한 나노크기의 TiO₂/SiO₂ 합성에서 물/계면활성제의 몰 비(W_(0)) 영향 및 그 광분해 특성

이만식,주창식,이근대,홍성수 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.4

나노크기의 TiO₂/SiO₂입자는 음이온 계면활성제 AOT(sodium bis(2-ethylhexyl) sulfosuccinate)를 사용하여 W/O 마이크로 에멀젼에서 TTIP(titanium isopropoxide)와 TEOS(tetraethylorthosilicate)의 가수분해 반응에 의해 제조하였다. 그때 TEOS의 몰분율은 0.1이였다. 나노입자 제조시 W_(0)(H₂O/AOT)비에 따른 열적안정성, 표면적, 결정성 및 결정크기 등과 같은 물리적 특성은 TEM, XRD, BET, FT-IR, TGA-DTA 등을 사용하여 분석하였다. 또한 광촉매적 특성을 알아보기 위해 회분식 반응장치를 이용하여 p-니트로페놀의 광분해 활성을 조사하였다. 제조된 Ti0₂/SiO₂ 나노입자는 열처리 온도 105℃, 300℃에서 비결정구조를 가졌으며, 소성온도 800℃에서는 Ti0₂ 결정입자 내에 무정형의 SiO₂로 인해 열적 안정성이 증가되었고, 여전히 anatase 결정을 유지하였다. 그리고 Ti0₂/SiO₂(90/10) 입자에서 SiO₂ 결정은 관찰되지 않았다. 제조된 촉매는 대체로 구형이며 아주 균일한 분포를 갖는 것으로 관찰되었다. W_(0) 비가 증가할수록 결정크기는 증가되었고, 표면적은 감소하는 경향을 나타내었다. 또한 p-니트로페놀에 대한 광분해 활성은 순수한 Ti0₂ 보다 Ti0₂/SiO₂(90/10) 촉매가 우수하였다. Nanosized TiO₂/SiO₂ particles were prepared by hydrolysis of TTIP (titanium isopropoxide) and TEOT (tetra-ethylorthosilicate) in sodium bis (₂-ethylhexyl)sulfosuccinate (AOT) reverse micelles. The mole fraction of TEOS was 0.1. The physical properties, such as surface area. thermal stability, crystallite size and crystallinity according to W_(0), ratio have been investigated by TEM, XRD, BET, FT-IR, TGA and DTA. In addition. the photocatalytic degradation of p-nitrophenol has been studied by using batch reactor in order to compare the photocatalytic activity of prepared nanosized TiO₂/SiO₂, particles. It is shown that the XRD pattern of the particle heat treated at 105℃ and 300℃ indicates amorphous and the major phase of all the prepared particles were anatase structure. No significant rutile phase was observed although the calcination temperature at 800℃ and no peaks SiO₂ crystal were also observed for TiO₂/SiO₂ (90/10). The presence of amorphous SiO₂ in TiO₂/SiO₂ particle enhanced the thermal stability of TiOz particle resulting in the suppression of the phase transformation from anatase to rutile phase. The crystallite size of prepared particles decreased with decreasing W_(0) ratio. The surface area increased with decreasing W_(0) ratio. In addition, TiO₂/SiO₂ (90/10) particles shows higher photoactivity than that of pure TiO₂ particles.

견관절 주위근의 스트레칭후 동통유발점압통역치의 변화

홍대종,최기섭,박시복,이상건 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.6