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Hong-Lin Xu,Guang-Hong Chen,Yu-Ting Wu,Ling-Peng Xie,Zhang-Bin Tan,Bin Liu,Hui-Jie Fan,Hong-Mei Chen,Gui-Qiong Huang,Min Liu,Ying-Chun Zhou 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1
Background: Panax ginseng Meyer (P. ginseng), a herb distributed in Korea, China and Japan, exerts benefits on diverse inflammatory conditions. However, the underlying mechanism and active ingredients remains largely unclear. Herein, we aimed to explore the active ingredients of P. ginseng against inflammation and elucidate underlying mechanisms. Methods: Inflammation model was constructed by lipopolysaccharide (LPS) in C57BL/6 mice and RAW264.7 macrophages. Molecular docking, molecular dynamics, surface plasmon resonance imaging (SPRi) and immunofluorescence were utilized to predict active component. Results: P. ginseng significantly inhibited LPS-induced lung injury and the expression of proinflammatory factors, including TNF-a, IL-6 and IL-1b. Additionally, P. ginseng blocked fluorescence-labeled LPS (LPS488) binding to the membranes of RAW264.7 macrophages, the phosphorylation of nuclear factor-kB (NF-kB) and mitogen-activated protein kinases (MAPKs). Furthermore, molecular docking demonstrated that ginsenoside Ro (GRo) docked into the LPS binding site of toll like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) complex. Molecular dynamic simulations showed that the MD2-GRo binding conformation was stable. SPRi demonstrated an excellent interaction between TLR4/MD2 complex and GRo (KD value of 1.16 × 10<SUP>-9</SUP> M). GRo significantly inhibited LPS488 binding to cell membranes. Further studies showed that GRo markedly suppressed LPS-triggered lung injury, the transcription and secretion levels of TNF-α, IL-6 and IL-1β. Moreover, the phosphorylation of NF-kB and MAPKs as well as the p65 subunit nuclear translocation were inhibited by GRo dose-dependently. Conclusion: Our results suggest that GRo exerts anti-inflammation actions by direct inhibition of TLR4 signaling pathway.
Gui Di Cai,Guang Hui Gao,Hong Yu Yang,Li Dan Zhu,Hua Liu,Guang Feng Wu,Ming Yao Zhang,Chao Zhou,Hui Xuan Zhang 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.3
Core–shell polybutadiene-graft-polystyrene rubber particles with different ratios of polybutadiene core to polystyrene shell were synthesized by an emulsion polymerization using K2S2O8 as an initiator. Then the core–shell rubber particles were blended with PS to prepare PS/PB-g-PS. The rubber particles with a size of 0.3–0.5 mm could toughen polystyrene significantly. The mechanical properties, morphologies and deformation mechanisms of samples were extensively investigated. The experimental results showed that the dispersion of rubber particles in a ‘‘cluster’’ state leads to better impact resistances. Crazing occurred from rubber particles and extended in a bridge-like manner to neighboring rubber particles parallel to the equatorial direction.
Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs
Zhang-Yong Ning,Xin-Tao Wu,Yan-Fen Cheng,Wen-Bao Qi,Yu-Fu An,Heng Wang,Gui-Hong Zhang,Shou-Jun Li 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.3
Reports of influenza A virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. Interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. Sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the human receptor. To date,there are no detailed data on the tissue distribution or histological features of either type of sialic acid-linked influenza virus receptors in beagle dogs, which are common laboratory animals and pets. We conducted the current study to visualize the in situ tissue distribution of both sialic acid-linked influenza virus receptors in various organs of beagle dogs using Maackia amurensis lectin II and Sambucus nigra agglutinin. Both α-2,3- and α-2,6-sialic acid-linked receptors were detected in the endothelial cells of the respiratory tract and other organs. Endothelial cells of most gastrointestinal organs were negative for α-2,3-sialic acid-linked receptors in the dogs. Our results suggested that these canine organs may be affected by influenza virus infection. The findings from our study will also help evaluate the occurrence and development of influenza virus infections in dogs.
Gui-Bao Wang,Hong-Zhong Huang,Yu Liu,Xiaoling Zhang,Zhonglai Wang 대한기계학회 2009 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.23 No.10
The article aims to estimate the uncertainty of possible failure events of redundancy systems based on the crossentropy (CE) method. Failure events of subsystems and components always result in the incomplete or complete failure of engineering systems, yet optimal condition monitoring of a complex system is heavily dependent on the accuracy analysis of all the failure events of subsystems and components and their interaction effects. The CE method is a versatile tool for estimating probabilities of rare events in complex systems with the least bias beyond conditional constraints. In this paper we introduce the CE method for analyzing the system reliability with the highest uncertainty among all possibilities satisfying supplied moment constraints, and developed numerical CE algorithms capable of estimating the uncertainty of failure modes in an M-dimensional redundancy system domain with moment constraints of order up to N. A general computational framework of event estimation and condition monitoring of redundancy systems is illustrated in which the Monte Carlo simulations and CE optimization algorithms are combined. Numerical results indicate potential improvements in the measure of the uncertainty of redundancy systems that would lead to the best-fit analysis of all the complete or incomplete failure events.
LU ZHANG,Peihui Yang,YAN-JIE GUO,JIAN-JUN LUO,GUI-MIN SUN,ZHI-HONG LIANG,YAN-JUAN TANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.3
A novel core – shell hybrid nanostructure was constructed by employing gold nanorod (AuNR)combined with rhodamine B (RB) as a core and silica as a shell. The poly(sodium 4-styr-enesulfonate) (PSS), a negatively charged polyelectrolyte, played the role of linker to electro-statically trap RB on AuNRs. Due to the °uorescence spectral overlap between RB and AuNRs at560 nm, the red °uorescence and enhanced green °uorescence of the hybrid nanostructures wereobserved obviously, which is capable for dual-color labeling. To reduce toxic side e®ects ofAuNRs, silica was coated on AuNRs as a shell to fabricate the novel core – shell hybrid nano-structure function as a dual-color labeling for cancer-cell imaging. The fabricated compositestructures were characterized by transmission electron microscopy (TEM), absorption spectrum,°uorescence spectrum, zeta potential measurements and laser scanning confocal microscope(LSCM). The experiment results con¯rmed that the obtained hybrid nanostructures providedexcellent photostability, biocompatibility and active surface for further biological functionali-zation. The novel composite structures may have great potential application in cell multicolorlabeling and imaging instead of traditional °uorescent dyes.
Wang, M.,Zhang, H.,Li, L.,Liu, X.,Hong, F.,Li, R.,Song, H.,Gui, M.,Shen, J.,Zhu, W.,Wang, J.,Zhou, L.,Jeong, J.H. Elsevier Sequoia 2014 JOURNAL OF ALLOYS AND COMPOUNDS Vol.585 No.-
Eu<SUP>3+</SUP> activated micrometer Y<SUB>2</SUB>MoO<SUB>6</SUB> phosphors with strong red emission bands, under a broad-band excitation wavelength range of 340-400nm, have been prepared by solid-state reaction and sol-gel technique. The photoluminescence indicates that the materials exhibit a characteristic red emission peak of Eu<SUP>3+</SUP> ions at 612nm. Compared with the material obtained by sol-gel method, the Y<SUB>2</SUB>MoO<SUB>6</SUB>:Eu prepared using solid state method showed much stronger red emission under the n-UV excitation. The broad excitation bands are assigned to charge transfer (CT) bands originating from the ligands (O) to the central ions Mo<SUP>6+</SUP>. About 12nm shift of excitation bands in Y<SUB>2</SUB>MoO<SUB>6</SUB>:Eu was found. With a decrease of the crystalline size, the excitation bands of O-Mo CT shift to the short wavelength. The origin of CT shift in macromaterial Y<SUB>2</SUB>MoO<SUB>6</SUB>:Eu was investigated quantitatively from the chemical bond viewpoint. All constituent chemical bonds in the crystal with or without oxygen vacancy were considered. The changes of average energy gap of the chemical bond Mo-O and the environmental factor (h<SUB>e</SUB>) surrounding Mo<SUP>6+</SUP> ions in the crystals were discussed quantitatively. Calculated results from two different methods analysis specifications showed that the origin of CT blue-shifts mainly come from the vacancies of O6 sites within the crystals.
Bi, Wei-Wei,Zhang, Wei-Hua,Yin, Gui-Hua,Luo, Hong,Wang, Shou-Qin,Wang, Hongran,Li, Chao,Yan, Wei-Qun,Nie, De-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14
Background: To determine the amount of co-expression of IDO and EGFR in breast cancer patients. Materials and Methods:In order to obtain the distribution of co-expression of IDO and EGFR in breast cancer, we tested 110 breast cancer paraffin tissue blocks with immunohistochemical methods. Then we investigated the relationship between the diagnostic and pathologic characteristics (tumor size, lymph node status, histologic grade, the gene expression of ER, PR, HER2, p53, Ki67 and PCNA) with the situation of co-expression of IDO and EGFR by reviewing the medical records of 32 breast cancer patients. Results: Among 110 breast cancers, 32 cases demonstrated IDO and EGFR co-expression (29.1%), IDO and EGFR synchronous co-expression being found in 19.1% and asynchronous in 10.0%. Conclusions: IDO and EGFR were co-expressed in breast cancer, including synchronous and asynchronous co-expression. The results suggest that considering IDO and EGFR as two indicators for breast cancer treatment or prognosis analysis provides a potential option of individual treatment for the portion of breast cancer patients with co-expression of IDO and EGFR.
Xi-yuan Zheng,Chang-hong Wang,Zi-jia Zhang,Gui-xin Chou,Tao Wu,Xue-mei Cheng,Zheng-tao Wang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.9
Acetylcholinesterase inhibitors (AChEIs) currently form the basis of the newest drugs available for the treatment of Alzheimer's disease. For the aim of screening effective AChEIs, the methanol extracts of the seeds of genus Peganum were found to show significant inhibitory activity of acetylcholinesterase enzyme (AChE) using an in vitro TLC-bioautographic assay. In further studies to seed of P. nigellastrum Bunge, activity-guided fractionation led to the isolation of two new alkaloids nigellastrine Ⅰ (9) and nigellastrine Ⅱ (10), and along with eight known alkaloids, vasicinone (1), vasicine (2), harmine (3), deoxyvasicinone (4), deoxyvasicine (5), harmaline (6), harmol (7), harman (8), in which harmol and harman were first isolated from species P. nigellastrum Bunge. As active constituents, all compounds showed good inhibitory activities against AChE. The results of in vitro semi-quality TLC-bioautographic assay showed that harmine, harmaline and harmol displayed a similar AChE inhibitive activities comparing to galanthamine. These results indicated that these alkaloids in P. nigellastrum Bunge could be a potent class of AChEIs.