Transcriptome analysis of the pheromone glands in Noorda blitealis reveals a novel AOX group of the superfamily Pyraloidea

Zhang Zu-Bing,Yin Ning-Na,Long Ji-Ming,Zhang Yong-Ke,Liu Nai-Yong,Zhu Jiaying 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

Noorda blitealis (Lepidoptera: Pyraloidea: Crambidae) is a major defoliating pest of Moringa trees. Focusing on its mating and reproduction, here we sequenced and analyzed the transcriptome of its pheromone glands (PGs) with a combination of Illumina sequencing, bioinformatics and phylogenetics approaches, coupled with a genomebased analysis. Transcriptome sequencing led to the yields of approximately 162 million clean reads, which were assembled into 60,578 unigenes and 121,692 transcripts, respectively. From the transcriptome, totally 117 genes encoding eight pheromone biosynthesis enzymes and one pheromone degradation enzyme were identified, 90 of which had complete open reading frames. A comparative analysis between PGs and bodies (removing PGs) revealed a large number of differentially expressed genes, including 79 pheromone biosynthesis and degradation related genes. Of the identified genes, NbliDES12 belonging to the △11 desaturase group was likely to a strong candidate for the desaturation of sex pheromones in N. blitealis, as implied by phylogenetic analyses and expression profiles. Finally and most notably, through genome and transcriptome analyses we discovered, for the first time, a novel aldehyde oxidase 6 (AOX6) group of the superfamily Pyraloidea that have been slightly expanded by gene duplications. Moreover, each orthologous AOX group shared highly conserved gene structure. Together, this current study has characterized the genes associated with sex pheromone biosynthesis and degradation from the PG transcriptome of N. blitealis, and more importantly, identifies a novel AOX group of the Pyraloidea.

Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs

Zhang-Yong Ning,Xin-Tao Wu,Yan-Fen Cheng,Wen-Bao Qi,Yu-Fu An,Heng Wang,Gui-Hong Zhang,Shou-Jun Li 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.3

Reports of influenza A virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. Interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. Sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the human receptor. To date,there are no detailed data on the tissue distribution or histological features of either type of sialic acid-linked influenza virus receptors in beagle dogs, which are common laboratory animals and pets. We conducted the current study to visualize the in situ tissue distribution of both sialic acid-linked influenza virus receptors in various organs of beagle dogs using Maackia amurensis lectin II and Sambucus nigra agglutinin. Both α-2,3- and α-2,6-sialic acid-linked receptors were detected in the endothelial cells of the respiratory tract and other organs. Endothelial cells of most gastrointestinal organs were negative for α-2,3-sialic acid-linked receptors in the dogs. Our results suggested that these canine organs may be affected by influenza virus infection. The findings from our study will also help evaluate the occurrence and development of influenza virus infections in dogs.

Comparative Analysis of Water Stress-Responsive Transcriptomes in Drought-Susceptible and -Tolerant Wheat (Triticum aestivum L.)

Yong Chun Li,Fan Rong Meng,Chun Yan Zhang,Ning Zhang,Ming Shan Sun,Jiang Ping Ren,Hong Bin Niu,Xiang Wang,Jun Yin 한국식물학회 2012 Journal of Plant Biology Vol.55 No.5

To understand better the mechanisms that regulate the water stress response in wheat, we conducted a comparative analysis of transcript profiles in roots from two wheat genotypes -- drought-tolerant ‘Luohan No. 2’ (LH)and drought-susceptible ‘Chinese Spring’ (CS). In LH roots,3831 transcripts displayed changes in expression of at least two-fold over the well-watered control when drought treatment was applied. Of these, 1593 were induced while 2238 were repressed. Relatively fewer transcripts were drought-responsive in CS; i.e., 1404 transcripts were induced and 1493 were repressed. In common between LH and CS,569 transcripts were induced and 424 transcripts were repressed. In all, 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories. Among those annotated transcripts from LH and CS that had fold-change ratios of at least 4, 92 induced transcripts were common to both, while 23 transcripts were specifically induced in LH. Gene ontology analysis of these induced genes showed highly significant enrichment for multiple terms related to abiotic stimuli, organic acid biosynthesis,and lipid metabolism. This suggests that these gene groups play important roles during the stress response in LH and CS, and might also be responsible for differences in drought tolerance between those genotypes.

A new approach for multi-channel surface EMG signal simulation

Yong Ning,Yingchun Zhang 대한의용생체공학회 2017 Biomedical Engineering Letters (BMEL) Vol.7 No.1

Simulation models are necessary for testing the performance of newly developed approaches before they can be applied to interpreting experimental data, especially when biomedical signals such as surface electromyogram (SEMG) signals are involved. A new and easily implementable surface EMG simulation model was developed in this study to simulate multi-channel SEMG signals. A single fiber action potential (SFAP) is represented by the sum of three Gaussian functions. SFAP waveforms can be modified by adjusting the amplitude and bandwidth of the Gaussian functions. SEMG signals were successfully simulated at different detected locations. Effects of the fiber depth, electrode position and conduction velocity of SFAP on motor unit action potential (MUAP) were illustrated. Results demonstrate that the easily implementable SEMG simulation approach developed in this study can be used to effectively simulate SEMG signals.

Seismic performance of RC columns retrofitted using high-strength steel strips under high axial compression ratios

Yong Yang,Ning Hao,Yicong Xue,Shiqiang Feng,Yunlong Yu,Shuchen Zhang 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.84 No.3

In this paper, the impact on seismic performance of an economical effective technique for retrofitting reinforced concrete (RC) columns using high-strength steel strips under high axial compression ratios was presented. The experimental program included a series of cyclic loading tests on one nonretrofitted control specimen and three retrofitted specimens. The effects of the axial compression ratio and spacing of the steel strips on the cyclic behavior of the specimens were studied. Based on the test results, the failure modes, hysteretic characteristics, strength and stiffness degradation, displacement ductility, and energy dissipation capacity of the specimens were analyzed in-depth. The analysis showed that the transverse confinement provided by the high-strength steel strips could effectively delay and restrain diagonal crack development and improve the failure mode, which was flexural-shear failure controlled by flexural failure with better ductility. The specimens retrofitted using high-strength steel strips showed more satisfactory seismic performance than the control specimen. The seismic performance and deformation capacity of the retrofitted RC columns increased with decreasing axial compression ratio and steel strip spacing. Based on the test results, a hysteretic model for RC columns that considers the transverse confinement of high-strength steel strips was then established. The hysteretic model showed good agreement with the experimental results, which verified the effectiveness of the proposed hysteretic model. Therefore, the aforementioned analysis can be used for the design of retrofitted RC columns.

Quantitative Analysis of Enlarged Cervical Lymph Nodes with Ultrasound Elastography

Zhang, Jun-Peng,Liu, Hua-Yan,Ning, Chun-Ping,Chong, Jing,Sun, Yong-Mei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Purpsoe: To investigate the diagnostic value of quantitative analysis of a tissue diffusion and virtual touch tissue imaging quantification (VTIQ) technique with acoustic radiation force impulse (ARFI) elastography for assessing enlarged cervical lymph nodes. Materials and Methods: Fifty-six enlarged cervical lymph nodes confirmed by pathologic diagnoses were covered in the study. According to the results of pathologic diagnosis, patients were classified into benign and malignant groups. All the patients were examined by both conventional ultrasonography and elastography. AREA% and shear wave velocity (SWV) in ROI of different groups were calculated and compared using ROC curves. Cut-off points of AREA% and SWV were determined with receiver operating characteristic curves. Results: Final histopathological results revealed 21 cases of benign and 35 cases of malignant lymph nodes. The mean values of AREA% and SWV in benign and malignant groups were $45.0{\pm}17.9%$ and $2.32{\pm}0.57m/s$, and $61.3{\pm}21.29%$ and $4.36{\pm}1.25$)m/s, respectively. For the parameters of elastography, "AREA%" and SWV demonstrated significant differences between groups (p=0.002). AREA% was positively correlated with SWV with a correlation coefficient of 0.809 (P<0.001). Conclusions: Stiffness of different lymph node diseases in patients may differ. Elastography can evaluate changes sensitively and provide valuable information to doctors. The study proved that the VTIQ elastography technique can play an important role in differential diagnosis of lymph nodes.

Evaluating the Green Efficiency and Determinants of Regional Economy in China

Yong-Rok Choi,Zhang Ning 한국환경경영학회 2010 한국환경경영학회 학술대회 Vol.2010 No.-

MAGED4 Expression in Glioma and Upregulation in Glioma Cell Lines with 5-Aza-2'-Deoxycytidine Treatment

Zhang, Qing-Mei,Shen, Ning,Xie, Sha,Bi, Shui-Qing,Luo, Bin,Lin, Yong-Da,Fu, Jun,Zhou, Su-Fang,Luo, Guo-Rong,Xie, Xiao-Xun,Xiao, Shao-Wen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Melanoma-associated antigen (MAGE) family genes have been considered as potentially promising targets for anticancer immunotherapy. MAGED4 was originally identified as a glioma-specific antigen. Current knowledge about MAGED4 expression in glioma is only based on mRNA analysis and MAGED4 protein expression has not been elucidated. In the present study, we investigated this point and found that MAGED4 mRNA and protein were absent or very lowly expressed in various normal tissues and glioma cell line SHG44, but overexpressed in glioma cell lines A172,U251,U87-MG as well as glioma tissues, with significant heterogeneity. Furthermore, MAGED4 protein expression was positively correlated with the glioma type and grade. We also found that the expression of MAGED4 inversely correlated with the overall methylation status of the MAGED4 promoter CpG island. Furthermore, when SHG44 and A172 with higher methylation were treated with the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZA-CdR) reactivation of MAGED4 mRNA was mediated by significant demethylation in SHG44 instead of A172. However, 5-AZA-CdR treatment had no effect on MAGED4 protein in both SHG44 and A172 cells. In conclusion, MAGED4 is frequently and highly expressed in glioma and is partly regulated by DNA methylation. The results suggest that MAGED4 might be a promising target for glioma immunotherapy combined with 5-AZA-CdR to enhance its expression and eliminate intratumor heterogeneity.

Predictive Potential of Glutathione S-Transferase Polymorphisms for Prognosis of Osteosarcoma Patients on Chemotherapy

Zhang, Shai-Lin,Mao, Ning-Fang,Sun, Jun-Ying,Shi, Zhi-Cai,Wang, Bing,Sun, Yong-Jian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objective: To evaluate the predictive value of glutathione S-transferase (GST) gene polymorphisms for the prognosis of osteosarcoma patients receiving chemotherapy. Methods: A total of 159 patients were included in our study between January 2005 and December 2007., with follow-up until January 2012. Genotyping was based upon the duplex polymerase-chain-reaction with the PCR-CTPP method. Results: At the time of diagnosis, 15.4% of the patients presented with metastasis, while 22.3% developed metastasis during follow-up. At the time of final analysis on January 2012, the median follow-up was 45.5 months. Patients with null GSTM1 and GSTT1 had a higher event free survival rate than non-null genotype, but no significant association was found between the two genotypes and prognosis of osteosarcoma. Individuals with GSTP1 Val/Val genotype tended to live shorter than with the IIe/IIe genotype, and we found a significantly higher risk of death from osteosarcoma (adjusted HR=2.35, 95% CI=1.13-4.85). Conclusion: The GSTP1 gene polymorphism may have an important role in the prognosis of osteosarcoma patients with chemotherapy. Further analyses with larger samples and more genes encoding metabolizing and DNA repair enzymes are warranted.

Original Article : The Research Regarding of Visitors` Perception of Animal Elements in Landscape Architecture

( Ning Shen ),( Jung Nam Bae ),( Li Qin Zhang ),( Yong Ji Piao ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.6

Based on the survey of East Lake Park, South Lake Park and Tian Waicun in Tai`an by questionnaire survey, on-the-spot investigation and in-depth interview, the paper analyzes the tendencies of visitors to the animal elements and animal landscape in landscape architecture from three terms: primary attributes, the cognition of animal elements and animal elements landscape preferences. We have drawn several conclusions: the landscapes of animal elements are in urgent demand by the public; The animal elements having lovely image, auspicious meaning and local characteristics are the most popular application types of animal elements landscapes; The most favorite types of animals and animal elements are: doves, cranes monkeys, pandas, butterflies, dragonflies, fold fish, dolphins, dragons and cartoon animals; The popular landscaping techniques of animal elements are the methods of combining with plants, buildings and garden ornaments.