Analysis of Physicochemical Properties and Oil Compounds of the Soil Near Kyuoungiu Railroad site

Bo-Hyun Kim,Eun-Hui Park,Goon-Taek Lee,Kye-Hoon Kim 한국토양비료학회 2005 한국토양비료학회 학술발표회 초록집 Vol.2005 No.9

Highly Efficient Agrobacterium Tumefaciens-medicated Transformation of a Forage Legume Medicago Trunctula Using Leaf Explants

Goon-Bo Kim,Seong-Wook Son,Ara Cho,Jeong-Hwan Mun 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.10

Upregulation of the serine palmitoyltransferase subunit SPTLC2 by endoplasmic reticulum stress inhibits the hepatic insulin response

Kim Goon-Tae,Devi Shivani,Sharma Amitesh,Cho Kyung-Hee,Kim Su-Jung,Kim Bo-Rahm,Kwon Sang-Ho,Park Tae-Sik 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Endoplasmic reticulum (ER) stress is induced by various conditions, such as inflammation and the presence of excess nutrients. Abnormal accumulation of unfolded proteins leads to the activation of a collective signaling cascade, termed the unfolded protein response (UPR). ER stress is reported to perturb hepatic insulin response metabolism while promoting insulin resistance. Here, we report that ER stress regulates the de novo biosynthesis of sphingolipids via the activation of serine palmitoyltransferase (SPT), a rate-limiting enzyme involved in the de novo biosynthesis of ceramides. We found that the expression levels of Sptlc1 and Sptlc2, the major SPT subunits, were upregulated and that the cellular concentrations of ceramide and dihydroceramide were elevated by acute ER stress inducers in primary hepatocytes and HepG2 cells. Sptlc2 was upregulated and ceramide levels were elevated by tunicamycin in the livers of C57BL/6J wild-type mice. Analysis of the Sptlc2 promoter demonstrated that the transcriptional activation of Sptlc2 was mediated by the spliced form of X-box binding protein 1 (sXBP1). Liver-specific Sptlc2 transgenic mice exhibited increased ceramide levels in the liver and elevated fasting glucose levels. The insulin response was reduced by the inhibition of the phosphorylation of insulin receptor β (IRβ). Collectively, these results demonstrate that ER stress induces activation of the de novo biosynthesis of ceramide and contributes to the progression of hepatic insulin resistance via the reduced phosphorylation of IRβ in hepatocytes.

재활용을 위한 플라스틱 부산물의 열분해 특성

김규보(Gyu-Bo Kim),김량균(Haak-Rho Lee),임호(Ryang-Goon Kim),박영도(Ho Lim),이학로(Young-Do Park) 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.6

토양분야 환경오염공정시험기준과 KS ISO규격의 일원화에 관한 연구

김지인 ( Ji In Kim ),김보경 ( Bo Kyong Kim ),김정화 ( Joung Hwa Kim ),이군택 ( Goon Teak Lee ),이상욱 ( Sang Uk Lee ),안문성 ( Mun Seong Ahn ),임태숙 ( Tae Sook Lim ),한진석 ( Jin Seok Han ),이원석 ( Won Seok Lee ) 한국환경분석학회 2012 환경분석과 독성보건 Vol.15 No.3

Korea has two type of the environmental official test methods, environmental standards enacted by ministry of environment (ES) and Korean industrial standard enacted by ministry of knowledge and Economy (KS), which causes the confusion of users, The main purpose of this study is to compare ES with KS and to make solutions to unify two types of standards. futhermore, We found the ways to improve ES and KS considering many countries aligned domestic standards with international standards. ES for soil quality consisted of sixty official test methods. We focused on thirty official test methods in ES except for the introduction, sampling methods and leak test methods and compared them with the corresponding KS just translated from ISO standard without any technical changes. By reviews and comparative tests between ES and KS we classified them into “No corresponding standards”, “Pre-unification completed”, “Pre-unification impossible”, “Unification completed” and “Unification impossible”. There were eighteen standards possible to unify, six standards impossible to unify and six standards corresponding no KS. We suggested that ESs for CN, phenols and Cr6+ were needed to adopt parts of the procedures in KS for improving recoveries and reducing the pre-treatment time and labor. We also found that both standards had to include detailed information about wavelength to analyze metals for user`s convenience.

사격장 토양의 화약성분 함량 분포 특성

조성현(Sung-Hyeon Cho),이군택(Goon-Taek Lee),김형돈(Hyoung Don Kim),김보현(Bo-Hyun Kim),김준영(Jun-Young Kim),한상재(Sang-Jae Han),김병일(Byung-Il Kim) 한국토목섬유학회 2011 한국지반신소재학회 학술발표회 Vol.2011 No.4

In the area of soil and groundwater environment, explosives are being highlighted. Therefore establishing the criteria for remedial works and developing the related technology are very important issues in the scope of nationwide research project. This investigation was carried out in military shooting area using explosives and focused on the determination of explosive compounds contents in soil and water. Because the domestic criteria have not established, the contents of explosive compound were compared with ROD(Record of Decision) of US EPA. Totally, 6 of 117 sampling point were contaminated and 4.3% of surface soil (0-10 ㎝) and 1.9% of subsoil (10-30) were contaminated. However, ROD value is too site specific according to site conditions to assess degree of contamination using simple comparison of analytical data with ROD. The results of this investigation are valuable for the selection of candidating sites for future precise investigation and risk assessment.

토양오염공정시험기준과 국제표준간의 분석방법 차이가 유류 정량에 미치는 영향

정인호,이군택,이원석,김용훈,김보현,김지인,김보경,Jung, In-Ho,Lee, Goon-Taek,Lee, Won-Seok,Kim, Yong-Hoon,Kim, Bo-Hyun,Kim, Ji-In,Kim, Bo-Kyong 한국지하수토양환경학회 2012 지하수토양환경 Vol.17 No.4

The main objective of this study is to assess the compatibility between Korean ministry of environment (KME) standard and ISO (KS I ISO) standard for the determination of BTEX and TPH content in soil. We carried out comparison analysis for both methods using CRM and matrix spiked samples. In case of GC-MS analysis for BTEX, we got statistically (significance level: 0.05) the same results from KME standard (ES 07600.1) and ISO standard (KS I ISO 15009). However, it showed statistically (significance level: 0.05) different results when TPH was analyzed by KME standard (ES 07552.1) and ISO standard (KS I ISO 16703). To clarify the reason why both methods produced different results for TPH content, we also did some additional experiments in terms of differences in extraction, clean-up and target hydrocarbon range. Extraction with polar and non-polar compounds mixed solvent (acetone+n-heptane) of KS I ISO 16703 showed higher extraction efficiency than with only non polar solvent (dichloromethane) extraction of ES 07552.1 by about 9%. While column type clean-up of KS I ISO 16703 showed the reduction in TPH content between before and after clean-up, batch type of clean-up of ES 07552.1 did not show any changes in TPH content through clean-up process. The target hydrocarbon range of ES 07552.1 and KS I ISO 16703 is $C_8{\sim}C_{40}$ and $C_{10}{\sim}C_{40}$, respectively. From this point of view, kerosene and JP-8 contaminated soil showed higher RPD (relative producibility deviation) values between results by both method than that of lubricant or diesel contaminated soil. The higher content of hydrocarbon ($C_8{\sim}C_{10}$) in kerosene and JP-8 played an important role in increasing RPD values in addition to the effects caused by different solvents and clean-up method. Consequently, it was concluded that both methods (ES 07552.1 and KS I ISO 16703) were not compatible.

全國 主要 都市에 錄陰樹로 植栽된 느티나무의 健康度 測定과 이를 利用한 大氣汚染狀態 推定

金君保,韓心熙,李景俊 서울大學校 農業生命科學大學 附屬樹木園 1995 서울大學校 樹木園 硏究報告 Vol.- No.15

本 調査는 大都市 및 工團地域에 綠陰樹로 植栽된 느티나무의 健康狀態를 測定하고, 이를 利用하여 大氣汚染狀態를 推定하기 위하여 實施되었다. 健康 診斷에 利用된 內容은 生長量, 잎의 可視的 被害, 葉綠素 含量, 카로테노이드 含量, 樹木 活力度이다. 1995年 8月 15日부터 21日까지 實施된 全國 調査에서는 3個의 山岳地域을 對照區(淸淨地域)로 하여, 6個의 大都市와 3個의 工團地域을 汚染地로 調査하였고, 서울市 調査에서는 總 19個 地域을 調査하였다. 生長量으로써 新梢 길이, 節間 길이, 잎의 크기 및 갯수, 그리고 잎의 可視的 被害와 樹皮酸度를 測定하였으며, 葉綠素와 카로테노이드 含量은 DMSO(dimethyl sulphoxide)로 抽出 후 分光分析器를 利用하여 測定하였다. 樹木 活力度는 Shigometer를 利用하여 形成層의 電氣 抵抗度로 表示하였다. 新梢生長量, 節間 길이, 잎의 개수의 測定 結果는 地域間 有意性이 認定되었으나 地域間, 個體間 變異가 심하여 일정한 傾向을 볼 수 없었으며, 樹皮 pH는 工團地域에서 가장 낮았다. 對照區를 제외한 全地域에서 可視的 被害가 나타났으며 특히 工團地域에서 심하였다. 葉綠素와 카로테노이드 含量, 樹木活力度는 山岳地域과 比較하여 大都市에서 낮고, 大氣汚染이 심한 工團地域에서 가장 낮았으며 統計的인 差異가 認定되었다. 樹木活力度는 葉綠素나 카로테노이드 含量과 높은 相關 關係를 보였으며, 특히 서울 地域에서 높은 相關係數(葉綠素 : r²=0.58, 카로테노이드 : r²=0.55)를 보였다. 健康度는 可視的 被害, 葉綠素와 카로테노이드 含量과 電氣 抵抗度의 네 가지 項目을 사용할 때 가장 信憑性 있게 測定할 수 있었다. 서울市 調査에서는 江南地域이 江北보다 被害 症狀이 컸으며, 특히 大氣汚染이 심한 구로 工團과 永登浦驛을 포함한 江南 西部 地域이 가장 심한 症狀을 보였다. 全國 느티나무의 健康度는 大氣汚染이 심할수록 낮게 나타났으며, 느티나무는 大氣汚染을 間接的으로 測定하는 指標植物로 活用할 수 있다고 結論 짓는다. This study was conducted to measure shade tree health in major cities and to test the possibility of using the health data to indirectly estimate status of air pollution in the same areas. Zelkova serrata Makino planted as a shade tree in six largest cities and three industrial complexes was measured in mid August for growth increment, visible leaf damage, bark pH, chlorophyll and β-carotene contents, and tree vitality. The results were compared with those of healthy trees in three mountain areas as a control. Cholorophyll and β-carotene were extracted in dimethyl sulphoxide solution and quantified by spectrophotometry, while tree vitality was estimated by measuring electrical resistance in the cambium using Shigometer. Growth parameters such as new shoot length, number of leaves, or width of annual rings varied widely in various locations. Visible leaf damage was observed in most of the sites except mountain areas, with heaviest damage in industrial complexes. Chlorophyll and β-carotene contents, bark pH, and tree vitality were lower in six major cities and lowest in industrial complexes, compared to those in mountain areas. Tree vitality was positively correlated with chlorophyll(r²=0.58) and β-carotene content(r²=0.55), and negatively correlated with degree of leaf damage. Tree health can be estimated most successfully by combining data on visible leaf damage, chlorophyll and β-carotene contents and electrical resistance. Among the 19 sites surveyed in Seoul metropolitan areas, west side of Kangnam(south of River Han) where Kuro and Yongdungpo industrial complexes were located showed lowest tree health and Kangnam showed lower tree health than Kangpuk(north of River Han). It is concluded that Zelkova serrata trees are sensitive to air pollution and can be used as a bioindicator to indirectly monitor air pollution status in large cities and industrial complexes.

Roles of medicago truncatula remorin genes in nodule- and arbuscular mycorrhizal symbioses

Goon Bo Kim,Young Woo Nam 한국육종학회 2010 한국육종학회 학술발표회 발표요지 Vol.42 No.1

후천성 면역 결핍증 환장서 발생한 뇌톡소플라즈마증 1례

김보현,이승익,이창훈,차승헌,이태홍,이선희,정주섭,조군제 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

뇌톡소플라즈마증은 후천성면역결핍증 환자에서 발생하는 중추신경계 기회감염증의 가장 흔한 원인 중의 하나이다. 그 빈도는 지역마다 다양하지만 우리나라에서는 매우 드물게 보고되고 있다. 지금까지 우리나라에서 보고된 후천성면역결핍증 환자에서 발생한 뇌톡소플라즈마증 증례들은 모두 혈청학적으로 진단한 것으로 조직학적으로 증명된 경우는 없었다. 저자들은 HIV 감염 후 약 10년 동안 특별한 치료를 받지 않고 지내다 뇌의 다발성 종괴로 내원하여 조직학적으로 확진된 뇌톡소플라즈마증 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Toxoplasrnosis is one of the most common opportunistic infection of the central nervous system in patients with acquired immunodeficiency syndrome(AIDS). There have been few reports of cerebral toxoplasmosis in patients with AIDS in Korea. In most cases, the diagnosis was assisted by serology and neuroradiologic findings. Making a reliable diagnosis of acute cerebral toxoplasmosis is difficult in patients with AIDS because of the lack of specificity of serologic data and neuroradiological findings. We report a case of 32-year-old man who presented with decreased mentality and fever. Brain MRI showed multiple ill-defined mass-like lesions in both basal ganglia and right thalamus. Stereotatic brain biopsy revealed small parasitic cysts which were filled with toxoplasmic bradyzoites in inflammatory brain tissue.