Survival Analysis of Oral Squamous Cell Carcinoma in a Subgroup of Young Patients

Fan, Yi,Zheng, Lei,Mao, Ming-Hui,Huang, Ming-Wei,Liu, Shu-Ming,Zhang, Jie,Li, Sheng-Lin,Zheng, Lei,Zhang, Jian-Guo Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Oral squamous cell carcinoma (OSCC) is predominantly a disease of middle-aged men with long-term exposure to tobacco and alcohol. An increasing trend has been reported at a younger age worldwide. Clinical records of 100 patients under the age of 45 years treated specifically for oral cavity SCC in our hospital during a 10-year period were retrospectively analyzed to calculate the survival rates. An obvious male predominance coincided with smoking trend among Chinese young individuals and female patients were more likely to have no traditional risk factors such as smoking or drinking. The 5-year overall survival rate and disease-free survival rate were 61.0% and 75.5%, respectively, consistent with other published series over the decade showing a relatively better survival among the young. No significant differences clearly correlated with outcome when comparing non-smokers non-drinkers to ever-smokers and ever drinkers (P>0.05). Overall survival rate and disease free survival rate was found to be significantly higher in patients with early-stage disease than with advanced stage disease (P=0.001, P=0.009 respectively). The strong influence of clinical stage on prognosis emphasizes the importance of early diagnosis and treatment of oral malignancies for this unique clinical subgroup.

Association of a novel antisense lncRNA TP73-AS1 polymorphisms and expression with colorectal cancer susceptibility and prognosis

Fan Jiayao,Xu Huiqing,Liu Bing,Jing Fangyuan,He Qingfang,Zheng Shasha,Shi Haining,Jiao Lefei,Fan Chunhong 한국유전학회 2022 Genes & Genomics Vol.44 No.7

Background: TP73-AS1 is a novel antisense long noncoding RNA and plays an important role in cell proliferation and cancer development. However, the link between TP73-AS1 and colorectal cancer (CRC) has not yet been reported. Objective: To explore the association of genetic variants in TP73-AS1 and its expression with CRC susceptibility and prognosis. Methods: A case-control study (including 507 CRC cases and 503 controls) and bioinformatics analysis were conducted. Results: rs9800 polymorphism was significantly related to higher risk in CRC [adjusted odds ratio (AOR) = 1.33, 95% confidence interval (CI) = 1.02-1.75, P = 0.034 in heterozygote codominant model]. There was no difference between TP73-AS1 polymorphisms and different tumor node metastasis (TNM) stages in the adjusted model. Moreover, TP73-AS1 expression level was positively related to different TNM stages. After adjusted for age, gender and TNM, higher TP73-AS1 expression levels were related to shorter recurrence-free survival time [hazard ratio (HR) = 1.66, 95% CI = 1.02-2.71, P = 0.043]. Conclusion: TP73-AS1 polymorphisms and expression may be associated with susceptibility and prognosis of CRC.

Bidirectional two-sample mendelian randomization analysis identifies causal associations between age at menarche and sarcopenia-related traits

Fan Wei,Gu Peng,Pu Bin,Yue Dan,Xin Qiao,Zheng XiaoHui,Zeng ZhanPeng,Fan Shaoyong 한국유전학회 2023 Genes & Genomics Vol.45 No.8

Background Observational studies have shown that the age of menarche is associated with sarcopenia, but confounding factors make the causal relationship difficult to infer. Objective Therefore, we conducted a bidirectional two-sample Mendelian randomized (MR) analysis to evaluate the potential causal relationship between age at menarche and sarcopenia-related traits (hand grip strength, lean mass, walking pace). Methods We obtained the latest aggregate statistics from the Genome-wide association studies (GWAS) database on the age of menarche of 182,416 participants from ReproGen, the appendicular lean mass of 244,730 participants from EMBL’s European Bioinformatics Institute, the left-hand grip strength of 401,026 participants, the right-hand grip strength of 461,089 participants and the usual walking pace of 459,915 participants from the UK Biobank. The inverse variance weighting (IVW) method and other MR methods were used to evaluate the bidirectional causal relationship between the age of menarche and sarcopenia. Results The forward MR results showed that the age of menarche predicted by the gene was positively correlated with left-hand grip strength (IVWβ=0.041, P = 2.00 × 10−10), right-hand grip strength (IVWβ=0.053, P = 1.97 × 10−18), appendicular lean mass (IVWβ=0.012, P = 4.38 × 10−13) and usual walking pace (IVWβ=0.033, P = 1.62 × 10−8).In the reverse MR analysis, we also found that the usual walking pace was positively correlated with the age of menarche predicted by genes (IVWβ=0.532, P = 1.65 × 10−4). Still, there was no causal relationship between grip strength and appendicular lean mass and the age at menarche. Conclusion Our results show that earlier menarche will increase the risk of sarcopenia. In addition, people with higher muscle function tend to have menarche later. These findings may provide a reference for prevention strategies and interventions for menarche in advance and sarcopenia.

Ratiometric and discriminative visualization of autophagic processes with a novel dual-responded lysosome-specific fluorescent probe

Fan Zheng,Yeshuo Ma,Jipeng Ding,Shuai Huang,Shengwang Zhang,Xueyan Huang,Bin Feng,Hongliang Zeng,Fei Chen,Wenbin Zeng 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Autophagy is a critical self-eating pathway involved in numerous physiological and pathological processes. Lysosomal degradation of dysfunctional organelles and invading microorganisms is central to the autophagy mechanism and essential for combating disease-related conditions. Therefore, monitoring fluctuations in the lysosomal microenvironment is vital for tracking the dynamic process of autophagy. Although much effort has been put into designing probes for measuring lysosomal viscosity or pH separately, there is a need to validate the concurrent imaging of the two elements to enhance the understanding of the dynamic progression of autophagy. Methods Probe HFI was synthesized in three steps and was developed to visualize changes in viscosity and pH within lysosomes for real-time autophagy tracking. Then, the spectrometric determination was carried out. Next, the probe was applied to image autophagy in cells under nutrient-deprivation or external stress. Additionally, the performance of HFI to monitor autophagy was employed to evaluate acetaminophen-induced liver injury. Results We constructed a ratiometric dual-responsive probe, HFI, with a large Stokes shift over 200 nm, dualwavelength emission, and small background interference. The ratiometric fluorescent signal (R = I610/I460) of HFI had an excellent correlation with both viscosity and pH. More importantly, high viscosity and low pH had a synergistic promotion effect on the emission intensity of HFI, which enabled it to specially lit lysosomes without disturbing the inherent microenvironment. We then successfully used HFI to monitor intracellular autophagy induced by starvation or drugs in real-time. Interestingly, HFI also enabled us to visualize the occurrence of autophagy in the liver tissue of a DILI model, as well as the reversible effect of hepatoprotective drugs on this event. Conclusions In this study, we developed the first ratiometric dual-responsive fluorescent probe, HFI, for real-time revealing autophagic details. It could image lysosomes with minimal perturbation to their inherent pH, allowing us to track changes in lysosomal viscosity and pH in living cells. Ultimately, HFI has great potential to serve as a useful indicator for autophagic changes in viscosity and pH in complex biological samples and can also be used to assess drug safety.

Effects of Chelated Copper and Zinc Supplementation on Growth Performance, Nutrient Digestibility, Blood Profiles, and Fecal Noxious Gas Emission in Weanling Pigs

( Zheng Fan Zhang ),( Jin Ho Cho ),( In Ho Kim ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.4

This study was conducted to evaluate the effects of chelated Cu and Zn on growth performance, nutrient digestibility, blood profiles, and fecal noxious gas emission in weanling pigs. A total of 90 weanling pigs with an initial body weight(BW) of 5.27 ± 0.04 kg were randomly allotted to two dietary treatments for 42 d. Pigs were then fed a control diet(CON) or a Zinpro diet (CON+0.1% chelate copper and zinc). There were nine replicate-pens with five pigs in each pen. During d 0 to 14 and d 14 to 28, the ADFI decreased(p<0.05) and the G/F increased(p<0.05) in pigs fed the Zinpro diet compared with those that received the CON diet. During d 28 to 42, the ADFI increased(p<0.05) in pigs fed the Zinpro diet relative to those fed the CON diet. Additionally, the apparent total tract digestibility of DM, N, and energy increased(p<0.05) in the Zinpro group when compared to the CON group on d 14 and 28. The lymphocyte percentage was also greater(p<0.05) in the Zinpro group than in the CON group. Overall, dietary supplementation with 0.1% chelate copper and zinc improved the growth performance and nutrient digestibility in weanling pigs.

Icefex: Protocol Format Extraction from IL-based Concolic Execution

( Fan Pan ),( Li-fa Wu ),( Zheng Hong ),( Hua-bo Li ),( Hai-guang Lai ),( Chen-hui Zheng ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.3

Protocol reverse engineering is useful for many security applications, including intelligent fuzzing, intrusion detection and fingerprint generation. Since manual reverse engineering is a time-consuming and tedious process, a number of automatic techniques have been proposed. However, the accuracy of these techniques is limited due to the complexity of binary instructions, and the derived formats have missed constraints that are critical for security applications. In this paper, we propose a new approach for protocol format extraction. Our approach reasons about only the evaluation behavior of a program on the input message from concolic execution, and enables field identification and constraint inference with high accuracy. Moreover, it performs binary analysis with low complexity by reducing modern instruction sets to BIL, a small, well-specified and architecture-independent language. We have implemented our approach into a system called Icefex and evaluated it over real-world implementations of DNS, eDonkey, FTP, HTTP and McAfee ePO protocols. Experimental results show that our approach is more accurate and effective at extracting protocol formats than other approaches.

Mapping the Most Massive Overdensities through Hydrogen (MAMMOTH). II. Discovery of the Extremely Massive Overdensity BOSS1441 at<i>z</i>= 2.32

Cai, Zheng,Fan, Xiaohui,Bian, Fuyan,Zabludoff, Ann,Yang, Yujin,Prochaska, J. Xavier,McGreer, Ian,Zheng, Zhen-Ya,Kashikawa, Nobunari,Wang, Ran,Frye, Brenda,Green, Richard,Jiang, Linhua American Astronomical Society 2017 The Astrophysical journal Vol.839 No.2

<P>Cosmological simulations suggest a strong correlation between high optical-depth Lya absorbers, which arise from the intergalactic medium, and 3D mass overdensities on scales of 10-30 h(-1) comoving Mpc. By examining the absorption spectra of similar to 80,000 QSO sight lines over a volume of 0.1 Gpc(3) in the Sloan Digital Sky Survey III (SDSS-III), we have identified an extreme overdensity, BOSS1441, which contains a rare group of strong Lya absorbers at z = 2.32 +/- 0.02. This absorber group is associated with six QSOs at the same redshift on a 30 comoving Mpc scale. Using Mayall/MOSAIC narrowband and broadband imaging, we detect Lya emitters (LAEs) down to 0.7 x L-Ly alpha ' * a and reveal a large-scale structure of LAEs in this field. Our follow-up LBT observations have spectroscopically confirmed 20 galaxies in the density peak. We show that BOSS1441 has an LAE overdensity of 10.8 +/- 2.6 on a 15 comoving Mpc scale, which could collapse to a massive cluster with M greater than or similar to 10(15) M-circle dot at z similar to 0. This overdensity is among the most massive large-scale structures at z similar to 2 discovered to date.</P>

Discovery of an Enormous Ly<i>α</i>Nebula in a Massive Galaxy Overdensity at<i>z</i>= 2.3

Cai, Zheng,Fan, Xiaohui,Yang, Yujin,Bian, Fuyan,Prochaska, J. Xavier,Zabludoff, Ann,McGreer, Ian,Zheng, Zhen-Ya,Green, Richard,Cantalupo, Sebastiano,Frye, Brenda,Hamden, Erika,Jiang, Linhua,Kashikawa, American Astronomical Society 2017 The Astrophysical journal Vol.837 No.1

<P>Enormous Ly alpha nebulae (ELANe), unique tracers of galaxy density peaks, are predicted to lie at the nodes and intersections of cosmic filamentary structures. Previous successful searches for ELANe have focused on wide-field narrowband surveys or have targeted known sources such as ultraluminous quasi-stellar objects (QSOs) or radio galaxies. Utilizing groups of coherently strong Ly alpha absorptions, we have developed a new method to identify high-redshift galaxy overdensities and have identified an extremely massive overdensity, BOSS1441, at z = 2-3. In its density peak, we discover an ELAN that is associated with a relatively faint continuum. To date, this object has the highest diffuse Ly alpha nebular luminosity of L-nebula = 5.1 +/- 0.1 x 10(44) erg s(-1). Above the 2 sigma surface brightness limit of SBLy alpha = 4.8 x 10(-18) erg s(-1) cm(-2) arcsec(-2), this nebula has an end-to-end spatial extent of 442 kpc. This radio-quiet source also has extended C IV lambda 1549 and He II lambda 1640 emission on greater than or similar to 30 kpc scales. Note that the Ly alpha, He II, and C IV emissions all have double-peaked line profiles. Each velocity component has an FWHM of approximate to 700-1000 km s(-1). We argue that this Lya nebula could be powered by shocks due to an active galactic nucleus-driven outflow or photoionization by a strongly obscured source.</P>

Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng

Fan, Ze-Yan,Miao, Cui-Ping,Qiao, Xin-Guo,Zheng, You-Kun,Chen, Hua-Hong,Chen, You-Wei,Xu, Li-Hua,Zhao, Li-Xing,Guan, Hui-Lin The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2

Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.

Characteristics of Mammary Paget's Disease in China: a National-wide Multicenter Retrospective Study During 1999-2008

Zheng, Shan,Song, Qing-Kun,Zhao, Lin,Huang, Rong,Sun, Li,Li, Jing,Fan, Jin-Hu,Zhang, Bao-Ning,Yang, Hong-Jian,Xu, Feng,Zhang, Bin,Qiao, You-Lin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The aim of this study was to detail characteristics of mammary Paget's disease (PD) representing the whole population in China. A total of 4211 female breast cancer inpatients at seven tertiary hospitals from seven representative geographical regions of China were collected randomly during 1999 to 2008. Data for demography, risk factors, diagnostic imaging test, physical examination and pathologic characters were surveyed and biomarker status was tested by immunohistochemistry. The differences of demography and risk factors between PD with breast cancer and other lesions were compared using Chi-square test or t-test, with attention to physical examination and pathological characters. The percentage of PD was 1.6% (68/4211) in all breast cancers. The mean age at diagnosis was 48.1, and 63.2% (43/68) patients were premenopausal. There is no difference in demography and risk factors between PD with breast cancer and other breast cancer (P > 0.05). The main pattern of PD in physical exam and pathologic pattern were patients presenting with a palpable mass in breast (65/68, 95.6%) and PD with underlying invasive cancer (82.4%, 56/68) respectively. The rate of multifocal disease was 7.4% (5/68). PD with invasive breast cancer showed larger tumor size, more multifocal disease, lower ER and PR expression and higher HER2 overexpression than those in other invasive breast cancer (P < 0.05). These results suggested that PD in China is a concomitant disease of breast cancer, and that PD with underlying invasive cancer has more multiple foci and more aggressive behavior compared with other breast invasive cancer. We address the urgent needs for establishing diagnostic and therapeutic guidelines for mammary PD in China.