배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

KRC 408, inhibitor of hepatic growth factor receptor (c-Met) suppress growth of gastric cancer through antiproliferation and antianigogenic antitumor activity

ZHENG Hong-Mei,HONG Sang-Won,CHOI Myung-Joo,KIM Da-Young,AHN Su-Mi,SHIN Moon-Geun,CHO Sung-Yun,KIM Hyoung-Rae,LEE Jong-Kook,LEE Don-Haeng,HONG Soon-Sun 大韓藥學會 2012 大韓藥學會 總會 및 學術大會 Vol.2012 No.1

The Protective Effect of Resveratrol on Dimethylnitrosamine-Induced Liver Fibrosis in Rats

Hong, Sang-Won,Jung, Kyung-Hee,Zheng, Hong-Mei,Lee, Hee-Seung,Suh, Jun-Kyu,Park, In-Suh,Lee, Don-Haeng,Hong, Soon-Sun 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.4

Oxidative stress in liver injury is a major pathogenetic factor in progress of liver fibrosis. Resveratrol, a representative antioxidant derived from grapes, has been reported to show widespread pharmacological properties. In this study, we investigated the protective effects of resveratrol on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. Rats were treated with resveratrol daily by oral gavage for seven days after a single intraperitoneal injection of DMN (40 mg/kg). Resveratrol remarkably recovered body and liver weight loss due to DMNinduced liver fibrosis. Liver histology showed that resveratrol alleviated the infiltration of inflammatory cells and fibrosis of liver tissue. Resveratrol decreased the level of malondialdehyde and increased the levels of glutathione peroxidase and superoxide dismutase. Also, resveratrol significantly inhibited the mRNA expression of inflammatory mediators including inducible nitric oxide, tumor necrosis factor-alpha and interleukin-1beta. In addition, resveratrol showed not only reduced mRNA expression of fibrosis-related genes such as transforming growth factor beta 1, collagen type I, and alpha-smooth muscle actin, but also a significant decrease of hydroxyproline in rats with DMN-induced liver fibrosis. Our results suggest that resveratrol could be used to treat liver injury and fibrosis and be useful in preventing the development of liver fibrosis and cirrhosis.

Biocatalysis and Fermentation Technology : Purification and Characterization of a Thermostable Xylanase from Paenibacillus sp. NF1 and its Application in Xylooligosaccharides Production

( Hong Chen Zheng ),( Ming Zhe Sun ),( Ling Cai Meng ),( Hai Sheng Pei ),( Xiu Qing Zhang ),( Zheng Yan ),( Wen Hui Zeng ),( Jing Sheng Zhang ),( Jin Rong Hu ),( Fu Ping Lu ),( Jun She Sun ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4

High levels of extracellular xylanase activity (211.79 IU/mg) produced by Paenibacillus sp. NF1 were detected when it was submerged-cultured. After three consecutive purification steps using Octyl-Sepharose, Sephadex G75, and Q-Sepharose columns, a thermostable xylanase (XynNF) was purified to homogeneity and showed a molecular mass of 37 kDa according to SDS-PAGE. The specific activity of the purified XynNF was up to 3,081.05 IU/mg with a 14.55-fold purification. The activity of XynNF was stimulated by Ca2+, Ba2+, DTT, and β-mercaptoethanol, but was inhibited by Fe3+, Zn2+, Fe2+, Cu2+, SDS, and EDTA. The purified XynNF displayed a greater affinity for oat spelt xylan with the maximal enzymatic activity at 60°C and pH 6.0. XynNF, which was shown to be cellulose-free, with high stability at high temperature (70°C-80°C) and low pH range (pH 4.0-7.0), is potentially valuable for various industrial applications. The enzyme hydrolyzed oat spelt xylan to yield mainly xylooligosaccharides (95.8%) of 2-4 degree of polymerization (DP2-4). Moreover, the majority of the xylooligosacharides (DP2- 4) products was xylobiose (61.5%). The thermostable xylanase (XynNF) thus seems potentially usefull in the production of xylooligosaccharides.

Optimization of Parameters for LCL Filter of Least Square Method Based Three-phase PWM Converter

Zheng, Hong,Liang, Zheng-feng,Li, Meng-shu,Li, Kai The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.4

LCL filters are widely used in three-phase PWM converter for its advantages of small volume, low cost and inhibition of high frequency current harmonic. However, it is difficult to optimize its design because its parameters are mutually influenced while the value of each parameter for LCL filter has impacts on the converter's cost and size. In this paper, the target of optimization is to minimize the parameter values of LCL filter, and an optimization method for parameters of LCL filter of three-phase PWM converter based on least square method is proposed. With this method, a quantitative calculation of the harmonic component of the converter’s side phase voltage is performed first, and then the quantitative relationship between phase voltage harmonics and grid phase current harmonics is analyzed. After that, the attenuation requirement of each harmonic is obtained by taking into account the requirements for each harmonic component of grid current. Then according to the optimization objective, the objective function with minimum harmonic attenuation deviation is established, and least squares method is adopted for three-dimensional global searching of parameters for LCL filter. Thus, the designed harmonic attenuation curve approximates the minimum attenuation requirements, and the optimized LCL filter parameters are obtained. Finally, the effectiveness of the method is verified by the experiments.

KRC-408, a novel c-Met inhibitor, suppresses cell proliferation and angiogenesis of gastric cancer

Hong, S.W.,Jung, K.H.,Park, B.H.,Zheng, H.M.,Lee, H.S.,Choi, M.J.,Yun, J.I.,Kang, N.S.,Lee, J.,Hong, S.S. Elsevier Science Ireland 2013 Cancer letters Vol.332 No.1

Among many cancer therapeutic targets, c-Met receptor tyrosine kinase has recently given particular attention. This kinase and its ligand, hepatocyte growth factor (HGF), play a central role in cell proliferation and the survival of several human cancers. Thus, we developed KRC-408 as a novel c-Met inhibitor and investigated its anti-cancer effects on human gastric cancer. KRC-408 inhibited the phosphorylation of c-Met and its constitutive downstream effectors such as phosphatidylinositol 3-kinase (PI3K), Akt, Mek, and Erk. This compound was found to exert anti-cancer effects stronger than those of 5-fluorouracil (5-FU) on gastric cancer cells, especially cell lines that overexpressed c-Met. Interestingly, cytotoxicity of KRC-408 was lower than that of 5-FU in normal gastric cells. Apoptosis induced by KRC-408 was accompanied by increased levels of cleaved caspase-3 and PARP as well as DNA condensation and fragmentation. Flow cytometry analysis showed an accumulation of gastric cancer cells in the G2/M phase with concomitant loss of cells in the S phase following treatment with this drug. In the angiogenesis studies, KRC-408 inhibited tube formation and migration of human umbilical vein endothelial cells (HUVECs), and suppressed microvessel sprouting from rat aortic rings ex vivo along with blood vessel formation in a Matrigel plug assay in mice. Results of an in vivo mouse xenograft experiment showed that the administration of KRC-408 significantly delayed tumor growth in a dose-dependent manner, and suppressed Akt and Erk phosphorylation as well CD34 expression in tumor tissues. These findings indicate that KCR-408 may exert anti-tumor effects by directly affecting tumor cell growth or survival via the c-Met receptor tyrosine kinase pathway. We therefore suggest that KRC-408 is a novel therapeutic candidate effective against gastric cancers that overexpress c-Met.

Click Synthesis of Hydrogels by Metal-Free 1,3-Dipolar Cycloaddition Reaction Between Maleimide and Azide Functionalized Polymers

Hong-Zheng Zhu,Gang Wang,Hong-Liang Wei,Hui-Juan Chu,Jing Zhu 한국고분자학회 2016 Macromolecular Research Vol.24 No.9

Herein, a metal-free 1,3-dipolar cycloaddition click reaction was used to prepare hydrogels. First, copolymer of N,N-dimethylacrylamide and 2-hydroxyethyl methacrylate was synthesized via free radical copolymerization with an initiator of 2,2'-azobisisobutyronitrile and a solution of 1,4-dioxane. Second, maleimide-functionalized polymer was obtained by a coupling reaction of the above synthesized copolymer and N-maleoyl alanine with a dehydrating agent of N,N'-dicyclohexylcarbodiimide. Similarly, azido-modified polymer was prepared by the reaction between azidoacetic acid and the as-prepared copolymer. Third, the hydrogels were obtained by the reaction of the azidomodified polymer and maleimide-functionalized copolymer without any initiator or catalyst. Gelation times of different conditions were measured by a cuvette inversion method. The samples of different steps were analysized by means of FTIR, NMR, SEM, etc. The swelling behaviour of the gels was investigated via a general gravimetric method. Finally, release mechanism was explored with a model drug molecule of 5-fluorouracil. It was found temperature, concentration and solvent had an impact on the gelation time. And the swelling behavior and drug-controlled release profiles of the hydrogels were relevant to the concentration of polymers used for the hydrogels.

Build modern logistic service chain based on logistic simulation

Hong-Xing Zheng,Xiang-Wen Li 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

To the logistic enterprises alliance that provide the modem logistic services, this paper firstly builds the integrated resources data warehouse based on the logistic resources meta data of the enterprises alliances. It also analyzes the primary factors that affect the enterprises alliance to build logistic services chain, and gives out the enterprises alliance services pattern that is based on the multi-layer service broker. However, the logistic resources scheduling rules based on market is given out. At last, adopting the above solution, this paper gives out the simulation process of an example, which is about one virtual enterprises alliance how to build one timely logistic service chain, and simulate by using ProModel software.

Improved Mutual MRAS Speed Identification Based on Back-EMF

Zheng, Hong,Zhao, Jiancheng,Liu, Liangzhong The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.3

In the design of sensorless control system for induction motor, high-precision speed estimation is one of the most difficult problems. To solve this problem, the common method is model reference adaptive method (MRAS). MRAS requires accurate motor parameters to estimate rotor speed precisely. However, when motor is running, the variety of temperature and magnetic saturation will lead to the change of motor parameters such as stator resistance and rotor resistance, which will lower the accuracy of the speed estimation. To improve the accuracy and rapidity of speed estimation, this paper analyses the mutual MRAS speed identification based on rotor flux linkage, and proposes an improved mutual MRAS speed identification based on back-EMF. The improved method is verified by Simulink simulation and motor experimental platform based on DSP2812. The results of simulation and experiment indicate that the method proposed by this paper can significantly improve the accuracy of speed identification, and speed up the response of identification.

Two-step enhanced cancer immunotherapy with engineered <i>Salmonella typhimurium</i> secreting heterologous flagellin

Zheng, Jin Hai,Nguyen, Vu H.,Jiang, Sheng-Nan,Park, Seung-Hwan,Tan, Wenzhi,Hong, Seol Hee,Shin, Myung Geun,Chung, Ik-Joo,Hong, Yeongjin,Bom, Hee-Seung,Choy, Hyon E.,Lee, Shee Eun,Rhee, Joon Haeng,Min, American Association for the Advancement of Scienc 2017 Science Translational Medicine Vol.9 No.376

<P>We report a method of cancer immunotherapy using an attenuated Salmonella typhimurium strain engineered to secrete Vibrio vulnificus flagellin B (FlaB) in tumor tissues. Engineered FlaB-secreting bacteria effectively suppressed tumor growth and metastasis inmousemodels and prolonged survival. By using Toll-like receptor 5 (TLR5)-negative colon cancer cell lines, we provided evidence that the FlaB-mediated tumor suppression upon bacterial colonization is associated with TLR5-mediated host reactions in the tumor microenvironment. These therapeutic effects were completely abrogated in TLR4 and MyD88 knockout mice, and partly in TLR5 knockout mice, indicating that TLR4 signaling is a requisite for tumor suppression mediated by FlaB-secreting bacteria, whereas TLR5 signaling augmented tumor-suppressive host reactions. Tumor microenvironment colonization by engineered Salmonella appeared to induce the infiltration of abundant immune cells such as monocytes/macrophages and neutrophils via TLR4 signaling. Subsequent secretion of FlaB from colonizing Salmonella resulted in phenotypic and functional activation of intratumoral macrophages with M1 phenotypes and a reciprocal reduction in M2-like suppressive activities. Together, these findings provide evidence that nonvirulent tumor-targeting bacteria releasing multiple TLR ligands can be used as cancer immunotherapeutics.</P>