Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

Lee, Jong-Uk,Cheong, Hyun Sub,Shim, Eun-Young,Bae, Da-Jeong,Chang, Hun Soo,Uh, Soo-Taek,Kim, Young Hoon,Park, Jong-Sook,Lee, Bora,Shin, Hyoung Doo,Park, Choon-Sik BioMed Central 2017 Respiratory research Vol.18 No.-

<P><B>Background</B></P><P>Idiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes.</P><P><B>Methods</B></P><P>Gene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting >2 absolute fold changes with <I>p</I>-values < 0.05. <I>CCL8</I> mRNA and protein levels were quantified using real-time PCR and ELISA. <I>CCL8</I> localization was evaluated by immunofluorescence staining.</P><P><B>Results</B></P><P>One hundred seventy eight genes differentially expressed and 15 genes exhibited >10-fold change. Among them, 13 were novel in relation with IPF. <I>CCL8</I> expression was 22.8-fold higher in IPF fibroblasts. The levels of <I>CCL8</I> mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (<I>p</I> = 0.022 and <I>p</I> = 0.026, respectively). The <I>CCL8</I> concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (<I>n</I> = 22), hypersensitivity pneumonitis (<I>n</I> = 20) and sarcoidosis (<I>n</I> = 19) (<I>p</I> < 0.005, respectively). Cut-off values of 2.29 pg/mL and 0.43 pg/mL possessed 80.2 and 70.7% accuracy for the discrimination of IPF from NC and the other lung diseases, respectively. IPF subjects with <I>CCL8</I> levels >28.61 pg/mL showed shorter survival compared to those with lower levels (<I>p</I> = 0.012). <I>CCL8</I> was expressed by α-SMA-positive cells in the interstitium of IPF.</P><P><B>Conclusions</B></P><P>Transcriptome analysis identified several novel IPF-related genes. Among them, <I>CCL8</I> is a candidate molecule for the differential diagnosis and prediction of survival.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12931-016-0493-6) contains supplementary material, which is available to authorized users.</P>

30% 과산화수소를 함유한 전문가 미백제의 효용성 평가

김신영,박재억,김창현,양성은 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.1

30% 과산화수소를 함유한 전문가 미백제인 레메화이트(Remewhite, Remedent Inc., Deurle, Belgium)를 시험군 피험자 31명, 시험용 미백제에서 과산화수소를 제외한 동일한 제재를 대조약으로 대조군 피험자 31명에게 1일 3회씩, 2차례에 걸쳐서 시행하였다. 미백의 임상적 효용성은 Colorimeter, Vitapan classical shade guide를 이용하여 전체 색변화량(□E*)을 측정하였다. 14주 후와 26주 후의 색변화량을 측정하여 군 간 색변화량을 비교하였고, 각 군 내에서의 색변화량도 비교하여 다음과 같은 결론을 얻었다. 1. Colohmeter를 이용한 평가시에 시험군에서 전체 색변화량(□E*)은 대조군에 비해 통계적으로 유의한 차이를 보였고, Vitapan classical shade guide를 이용한 평가시에는 통계적으로 유의한 차이를 나타내지 않았다(p < 0.05). 2. 시간이 지남에 따라 색 복원 현상이 나타남에도 불구하고 미백 시행 14주 후와 26주 후에도 미백의 효과가 유지된다고 할수 있다. 3. Colohmeter를 이용한 평가시에 Vitapan classical shade guide를 이용한 평가시보다 작은 색변화량을 더 정확하게 측정할 수 있다. This study evaluated the efficacy of an office bleaching gel (RemeWhite, Remedent Inc., Deurle, Belgium) containing 30% hydrogen peroxide. 31 volunteers were recieved office bleaching with the RemeWhite for 3 times at one visit, total 2 visits. As control group, the same gel in which hydrogen peroxide was not included was applied to 31 volunteers with the same protocol. The shade change (□E*, color difference) of 12 anterior teeth was measured using Colorimerter and Vitapan classical shade guide. The shade change of overall teeth in the experimental group was significantly greater than that in the control group which was measured using Colorimeter. There was also a significant difference between baseline and 14 weeks or 26 weeks though color rebounding phenomenon occurred as time went by. Small shade change difference can be measured accurately using Colorimeter than using Vitapan classical shade guide.

산업체 만족도 제고를 통한 인턴십 활성화 방안

임창옥,최은영 호남대학교 대학원 2008 호남대학교 대학원 논문집 Vol.6 No.-

In-depth survey was performed for the industry which participated in internship program of HONAM university culture industry professional training business team to learn the opinion and satisfaction for program operation of anticipated colleges and student’s ability for industry’s desire satisfaction which is the most demander for professionals and to build basics for establishment of steady industrial-educational cooperation panning WIN-WIN strategy. As a result of the survey analysis, it is concluded that assistance to the trainee, steady holding industrial-educational seminar, extension of hands-on trainings, diversity of programs and needs for central control center are needed to vitalize the internship program In the future, internship operation time and period should be decided through a proper communication with the industry, and it is required that the program should satisfy both the experienced students and the industry.

간호사의 직무 스트레스와 이직 간의 관련성

이은희,장세진,김현옥,노재훈,박은정,원종욱 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.2

우리나라 지역 직업성질환 감시체계의 현황과 전망

임종한,장성실,김성아,문재동,채창호,홍윤철,김수영,김진석,김영욱,한상환,이혜숙,원종욱,송동빈,하은희,강성규 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

기존의 특수건강진단과 작업환경측정을 통한 직업병 관리가 진폐증, 소음성난청 등의 소수 특정질환에 국한되고 실제 직업병 발생 규모 파악이나 신종 직업병의 발견에 한계를 보인다는 사실은 산업의학전문가들 사이에서도 공감을 이루고 있다. 미국과 영국 등에서의 직업성질환 감시체계에 대한 경험은 우리 나라의 직업성질환 감시체계 구축에도 새로운 자극제가 되면서, 1998년이후 인천, 대전, 여천, 구미, 부울경 지역에서 지역 직업성질환감시체계를 산업보건관리의 중요한 시스템으로 구축하려는 노력이 확산되고 있다. 새로이 구축되어지는 이들 지역 직업성질환 감시체계는 감시하고자 하는 대상질환, 활용 가능한 인적자원 및 자료원, 지역 의료체계의 특수성 등에 따라서 목적과 방법을 달리하면서 독특한 형태로 발전을 하고 있다. 각 지역단위 감시체계들이 그 상황에 맞게 독특한 목적과 전략들을 발전 시키면서도, 향후 발전할 국가적인 차원의 직업성질환 감시체계 구축을 위하여 직업성질환 감시의 기본 전략 등을 공유하는 등의 노력이 필요하다. 환례 정의 및 기본적인 등록 서식의 공유, 직업성질환 감시 자료원의 발굴, 공동의 정보 네트워크 및 직업성질환 감시 데이터베이스 구축 등 직업성질환 감시활동을 지원하기 위한 여러 기초 인프라 구축에 힘을 모아야 할 것이다. 우리 나라에서 직업성질환 감시체계를 성공적으로 구축하기 위해서는 수집된 자료의 질 관리를 위한 직업성질환 감시의 원칙 제정과 감시 전략의 공유 등이 필요하며, 전국적인 직업성질환 감시체계의 하부구조라고 할 수 있는 지역감시체계의 기초 토대 마련과 강화작업이 절실하게 필요하다.

화소 기반 형태분석 방법을 이용한 알츠하이머 치매환자의 회백질 용적감소의 정량적 분석

임현국,최은형,이창욱,Lim, Hyun-Kook,Choi, Eun-Hyung,Lee, Chang-Uk 대한생물정신의학회 2008 생물정신의학 Vol.15 No.2

Objectives : The purpose of this study was to find brain regions in which gray matter volume was reduced and to show the capability of voxel-based morphpmetry(VBM) analysis for lateralizing clinically significant brain regions in dementia of Alzheimer's type patients compared to healthy group. Methods : MR T1-weighted images of the 20 dementia of Alzheimer's type patients were compared with those of the 20 normal controls. Images were transformed to standard MNI space. In order to observe gray matter volume change. Gray matter was smoothed with a Gaussian kernel. After these preprocessing, statistical analysis was performed using statistical parametric mapping software(SPM2). Results : Gray matter volume was significantly reduced in the bilateral parahippocampal gyri, Lt. anterior cingulate gyrus, Lt. posterior cingulate gyrus, bilateral superior temporal gyri Lt. middle temporal gyrus, Lt. superior, bilateral middle, Rt. anterior frontal gyri and Rt. precuneus in dementia of Alzheimer's type patient group. Conclusions : These VBM results confirm previous findings of temporal lobe and limbic lobe atrophic changes in dementia of Alzheimer's type, and suggest that these abnormalities may be confined to specific sites within that lobe, rather than showing a widespread distribution.

지역4-H조직의 내적 적합성에 관한 문화기술적 연구

김진모,김진군,전영욱,이정은,최영창,이은영 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.2

이 연구의 목적은 지역4-H조직의 내적 적합성을 탐색하고 이를 기술하는데 있었다. 2006년 8월부터 10월까지 약 3개월 동안 지역4-H후원회 회장 및 임원진 3명, 지역4-H연맹 회장 1명, 학교4-H 지도교사 4명, 지역의 영농4-H회장 1명, 시·군 농업기술센터 소장 및 4-H담당자 9명을 주요 연구 참가자로 하여 심층면담을 통해 자료를 수집하였다. 수집한 자료를 분석한 결과는 다음과 같았다. 첫째, 지역4-H조직 관련자들이 인식하는 지역4-H조직의 주요성과 격차로, ① 민간4-H조직을 중심으로 한 자율적이고 독립적인 활동 부재, ② 지역4-H조직 간 다양한 종류의 협력 부재, ③ 지역사회에서 4-H활동에 대한 가치 인식 부족이 나타났다. 둘째, 지역4-H조직의 핵심 과업으로 ① 4-H의 가치 및 이념에 부합하고, 지역의 실정에 맞는 프로그램 확보, ② 지역4-H 조직 간 협력적 네트워크 확립, ③ 4-H에 대한 지역사회의 관심 및 지지 확보가 나타났다. 셋째, 지역4-H조직의 내적 부적합성으로는 ① 인원 확보의 어려움과 핵심과업에 대한 전문성 결여, ② 관료화되고 경직된 낡은 문화, ③ 조직적 자원의 축소 및 조직 간 낮은 네트워크의 수준이 도출되었다. The purpose of this study was to describe and understand internal fit in Local 4-H Organization. The main participants of this study were one leader and two members of Local 4-H Society for the support, one leader of Local 4-H Association, four School 4-H teacher, one leader of Local farm 4-H, 9 local agricultural extension workers. The data were collected through in-depth interview with them from August, 2006 to October, 2006. The Results from this study were as follow: 1. Key performance gaps of local 4-H organizations were identified: ① lack of local 4-H based activities, ② lack of various types of partnership between local 4-H organizations, ③ lack of understanding value of 4-H activities in the communities. 2. Key tasks of local 4-H organizations were identified: ① developing local 4-H programs based on 4-H's values and ideas, ② establishing the cooperative networks among local 4-H organizations, ③ ensuring the interest and support about 4-H in the communities. 3. Internal misalignments of local 4-H organizations were identified: ① difference of recruitment and lack of expertise, ② bureaucratic and rigid cultures, ③ decrease in organizational support and low level of network among organizations.

Multiple myeloma 환자에서 Bortezomib치료 후 발생한 마비성 장폐색증 1례

김준영,이원식,손창학,박성길,진한영,박석주,조영완,정은욱,강명주,박정하,주영돈 인제대학교 2006 仁濟醫學 Vol.27 No.-

Bortezomib is the first clinically available proteasome inhibitor that is clinically effective in the treatment of multiple myeloma. A proteasome inhibitor acts through multiple mechanisms to arrest tumor growth, tumor spread, and angiogenesis. The main adverse effects of bortezomib are gastrointestinal symptoms, cytopenia, fatigue, and peripheral neuropathy. To date, severe paralytic ileus has not been reported as a toxic effect of bortezomib treatment in multiple myeloma. Bortezomib is a novel agent that has only been used clinically for 30 months, so a need exists to further evaluate its toxicity. We report a case of grade Ⅲ (NCI CTCAE v3.0) or grade Ⅳ(SWOG toxicity criteria) paralytic ileus in a 65-year-old man with relapsed multiple myeloma who underwent one cycle of single-agent bortezomib treatment scheduled in a 21-day cycle (1.3 mg/㎥ as a single i.v. bolus on days 1, 4, 8, and 11).

우리나라 인삼 육종의 주요 성과와 전망

방경환(Kyong-Hwan Bang),김영창(Young-Chang Kim),이정우(Jung-Woo Lee),조익현(Ick-Hyun Cho),홍지은(Chi-Eun Hong),현동윤(Dong-Yun Hyun),김장욱(Jang-Uk Kim) 한국육종학회 2020 한국육종학회지 Vol.52 No.S

Artificial selection of ginseng has been practiced since Hwangsook (with yellow pericarp and a green stalk, and was developed from a landrace parent) and Cheonggyeong (with red pericarp) were selected as breeding lines in 1926. Systematic research into ginseng breeding, however, started in earnest in the 1960s when the Central Research Institute of Monopoly and Technology (CRIMT) was established, and the Korean Ginseng Experiment Station was organized under the CRIMT. Research into variant characteristics, resource collections, and genetic evaluations began around this time. With the establishment of the Korean Ginseng Institute in the 1970s, studies involving pedigree selection, cataloguing of agricultural traits of genetic resources, generation shortening by tissue culture, and heritability assessments were conducted. In the 1980s, regional adaptation tests were carried out on breeding lines, focusing on ginseng-producing districts. In the 1990s, research was performed on seed multiplication for variety diffusion, effective components and processing quality, and cross breeding. Foreign ginsengs were introduced for interspecies hybridization, and studies were conducted using genetic engineering techniques. Since the 2000s, applications have been made to patent different ginseng cultivars. Currently, 32 cultivars are registered at the Korea Seed & Variety Service. Future goals for ginseng breeding include developing climate change- and disaster-resistant, consumer-oriented, high-performance cultivars. Therefore, it is necessary to develop technologies for distributing new cultivars by collecting and evaluating genetic resources, and cross breeding and performing mass propagation using these resources.

병원구매업무에 있어서의 전자상거래 도입에 관한 연구 : 구매부서 관리자의 인식도를 중심으로 Focused on the Understanding of Purchasing Managers

황은범 ( Eun Bum Hwang ),남상요 ( Sang Yo Nam ),하호욱 ( Ho Uk Ha ),이창은 ( Chang Eun Lee ) 한국병원경영학회 2001 병원경영학회지 Vol.6 No.3

This study is focused that the electronic commerce(EC) on the purchasing section may improve the efficiency and transparency of the hospitals management. After reviewing the purchasing activity of hospitals, I study the introduction, expected effects, and problems of EC. So, I am going to provide basic information for activating EC. The samples are managers of 170 hospitals, which are located on Seoul. As a result of collection this survey, I analyze 79 hospitals. For data analysis, I use X^2-test and ANOVA for purchasing management and the relevance of EC according to the level of care. The results of this study are 1. the problems on the management of purchasing section are : firstly, they don't have sufficient time to study market. Secondly, it is difficult to find competitive suppliers. And, lastly, they cannot gather a lot of information about the price of products. 2. There are many answers of the needs on the introduction of B2B. However, some hospitals think they don't need it. But, the most answers are that the EC will be settled within 4 years. So, we can realize that these hospitals are getting interested on the EC. On the other hand, I find that they prefer outside EC companies for the introduction of EC. 3. On the expected effects on EC, first is the effectiveness of the market survey. The next is to collect information of adequate price of products owing to clear transaction, find easier new suppliers and gather useful data. 4. On the external problems of the introduction of EC, there is low credibility related to the security and the weakness of suppliers' information system. Especially, on the Real Transaction Price Payment system, the bigger bed size, the higher understanding on these problems. On the internal problems of the introduction of EC, first is the burden of the introduction of EC and operating cost. Especially, on the burden of the disclosure of revenue source, the smaller bed size, the higher understanding on this problem. So, this is a point which deserves my attention statistically. However, this shows relatively little understanding about incomplete the standard of product category and the weak information system of hospital. Through this study, I am going to suggest 3 points for the activation of the introduction of EC on hospitals. 1. The reform of the Real Transaction Price Payment System on medical supplies and materials for medical treatment. 2. The establishment of the standard of product category. 3. The promotion of information system based on network.