In situ hybridization기법을 이용한 shiga toxin-producing Escherichia coli의 검출 및 stx2의 염기서열분석

김의종,이동영,최혜심,주세익,이정희,김상현,반성환 대한임상미샐물학회 2000 Annals of clinical microbiology Vol.3 No.2

혈액에서 분리된 세균의 항생제감수성 : Vitek System을 이용한 최소억제농도측정 Minimal Inhibitory Concentration Determined by Vitek System

김의종,김선주,신보문,박명희,조한익,김상인 대한화학요법학회 1988 대한화학요법학회지 Vol.6 No.1

Minimal inhibitory concentrations of total 148 strains isolated from blood culture at Seoul National University Hospital were determined by Vitek System. 90% MIC of gram-negative bacteria against amikacin was 2-16㎍/㎖, and those against beta-lactams was > 16㎍/㎖ in general, Except Salmonella spp,. 90% MIC against chloramphenicol and tetracycline was >8㎍/㎖. Range of 90% MIC against vancomycin was ≤.5-4㎍/㎖ in case of garm-positive bacteria, except Enterococcus spp. which showed 90% MIC as >16㎍/㎖. Against ampicillin, 90% MIC of Staphylococcus spp. was >16㎍/㎖, but those of Streptococcus spp. was ≤.25-2㎍/㎖. Streptococcus viridans group showed 90%MIC against penicillin as ≤.03㎍/㎖. Susceptibility of gram-negative bacteria was generally 98% against amikacin, and those of gram-positive bacteria was 80% against vancomycin. 34% of Staphylococcus spp. was resistant of oxacillin. These results may be useful for the primary selection and the dosage determination of antibiotics in the treatment of patients with bacteremia.

원자로압력용기장 용접열영향부의 미세조직 변화가 Magnetic Barkhausen Noise 거동에 미치는 영향

김주학,윤의박,문종걸,박덕근,홍준화 韓國非破壞檢査學會 1998 한국비파괴검사학회지 Vol.18 No.4

최근 자기적 특성의 변화가 재료의 미세조직적 상태에 민감하다는 보고가 있다. 재료의 미세조직은 상(phase), 결정립의 크기, 석출물 등의 다양한 인자로 구성되며, 본 연구에서는 이러한 인자들을 이용하여 재료의 특성과 Barkhausen noise(BN)의 관계를 연관시키기 위한 시도를 하였다. ASTM A 508 Gr.3 원자로압력용기강을 대상으로 용접열영향부의 미세조직적인 변화를 일곱가지 대표적인 영역으로 구분하였고, 각 영역에서의 미세조직과 기계적특성의 변화를 파악한 후, 각 조건별로 BN을 측정하였다. 통상의 보자력이나 잔류자화 같은 자기적 성질은 큰 변화가 없었지만, BN은 미세조직의 상태에 따라 현격한 변화를 보였다. 결정립과 석출물의 크기가 증가함에 따라 BN은 증가하였고, tempered martensite 보다는 tempered bainite 조직에서 높은 BN을 나타냈다. Recent study has demonstrated that some magnetic properties are sensitive to the microstructural state of material. The ASTM A 508 Gr. 3 reactor pressure vessel steel has various microstructural changes including martensitic and bainitic phases, and various sizes of grain and precipitates in the weld heat-affected zone (HAZ). To correlate the microstructural state with Barkhausen noise (BN), specimens were prepared through simulating various weld thermal cycles using a thermal simulator. The conventional magnetic properties, i.e. coercive force, remanence and maximum induction, did not change significantly. whereas the BN amplitude and energy during a magnetization cycle changed markedly with microstructural state. The BN increased with increasing grain and carbide sizes, and the tempered bainite structure showed higher BN parameter than tempered martensite.

短期所得林産物 市場接近物量의 輸入管理制度 改善方案

金義慶,鄭秉憲,李聖淵,朱麟源 한국산림경제학회 2002 산림경제연구 Vol.10 No.2

본 연구는 밤, 잣, 대추에 대하여 이루어지고 있는 최소시장접근물량에 대한 수입관리제도의 문제점을 살펴보고 그 개선방안을 도출하는데 목적이 있다. 주요 결과를 보면 밤의 경우 1995년 이후 평균 10%정도의 낮은 수입이행률을 보이고 있으며, 잣의 경우 환율 급변에 의한 환차손으로 수입관리기관이 손해를 보거나 시장조절기능도 미약하다는 문제점을 가지고 있는 것으로 나타났다. 따라서, 수입관리방식은 잣의 경우 현재의 국영무역에서 수입권공매방식으로, 밤의 경우 현재의 수입권공매방식을 선착순배정방식이나 수입허가제방식으로 바꾸는 것이 바람직한 것으로 분석되었다. 또한, 수입시기를 국내산 성출하시기를 피하여 수입하는 것이 바람직하며, 수입냉동품, 세관검사 및 저가신고에 대한 적절한 수입관리대책도 필요한 것으로 나타났다. The objectives of this study are to analyze the problems of import administration system for minimum market access of chestnut, Korean pine nut and jujube, and suggest its improvement measures. Major findings are as follows : The fill rate of minimum market access for chestnut was very low of about 10% since 1995. The import administration agency of Korean pine nut suffered the loss from abrupt exchange rate change and had weak market control, Therefore it was suggested that Quota Auction System is better than current State Trading System for Korean pine nut, and First-Come First-Served System or Import License System is better than current Quota Auction System for chestnut. And it is desirable to import during the period beyond the main harvesting season for domestic products, and needed to construct the adequate aministration measures for forzed product import, customs examination and low price declaration.

악관절 융기 증강술을 통한 습관성 악관절 탈구환자의 치험례

김일규,신주호,오성섭,최진호,김형돈,오남식,김의성 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

Recurrent mandibular dislocation is frequent morbidity of temporomandibular joint relatively. There are many etiologic causes in TMJ disorders but, difficult to find obvious one. Various treatment methods have been utilized for a mandibular dislocation. It is categorized into two groups broadly - nonsurgical or surgical methods. The basic rationale of the surgical method is to allow free movement of the condyle by reducing height of articular eminence or to limit anterior excessive movement of the condyle by increasing height of articular eminence or soft tissue anchoring procedure. In this case, 69 year-old woman was treated by augmentation of the articular eminence with mandibular symphysial bone graft leading to osteosynthesis without difficulty. As a result, favorable postoperative outcome was obtained functionally without any complication or recurrence.

악관절 수술후 안면신경 손상에 대한 임상적 연구

주재동,이의웅,김준배,박광호,김형곤 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.4

Authors have studied retrospectively the facial nerve injury after TMJ surgery through the preauricular approach routine. The study material used was 4 patients of all 113 patients who were diagnosed as internal derangement and have been operated from March 1989 to February 1991 in Youngdong severance hospital, and were induced postoperatived facial nerve injury. The patient group who had the postoperative injured facial nerve was recognized degree of injury using the diagnostic method, Electromyography(EMG) and Nerve conduction test(NCT) which are used widely at present and was treated as conservative care and we identified the recovery time as the same method. The results as follows : 1. The meticulous care and precious surgical technique are needed in both operation and postoperation. During the TMJ surgery, the excessive retraction of the flap and frequent use of nerve stimulator and electric surgical knife should be avoided as possible and postoperative hematoma and swelling should be minimized. 2. The 4 patients were experienced with the postoperative facial nerve injury of all 133 patients who had been operated the TMJ surgery through the routine preauricular approach on our hospital. And the incidence of postoperative facial nerve injury happened was about 0.3% and its incidence was relatively low comparing with any other previous reports. 3. EMG and NCT were considered as useful methods which can diagnose the nerve injury objectively and identified the effect of treatment and recovery time. 4. The faical nerve-injured patients who were induced postoperatively after TMJ surgery, were diagnosed as second-degree nere injury through the EMG and NCT. And the patient group was treated well as conservative physical therapy for about 2 to 4 months.

Cone beam형 전산화단층영상을 이용한 하악과두 위치의 연구

황형주,김규태,최용석,황의환 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.2

Purpose : To evaluate position of the mandibular condyle within articular fossa in an asymptomatic population radiographically by a cone beam computed tomography. Materials and Methods : Cone beam computed tomography of 60 temporomandibular joints was performed on 15 males and 15 females with no history of any temporomandibular disorders, or any other orthodontic or prosthodontic treatments. Position of mandibular condyle within articular fossa at centric occlusion was evaluated. A statistical evaluation was done using a SPSS. Results : In the sagittal views, mandibular condyle within articular fossa was posteriorly located at medial and central sections. In the coronal views, mandibular condyle within articular fossa was laterally located at central section. Mandibular condyles in the right and left sides were showed asymmetric positional relationship at medial, central, and lateral sections. Conclusion : Mandibular condyle within articular fossa in an asymptomatic population was observed nonconcentric position in the sagittal and coronal views.

삼백초(Saururus Chinensis (Lour.) Bail) 열수추출물의 암세포 저해 효과 및 항산화효소 활성에 미치는 영향

양의주,한정,김현정,이인선 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

삼백초(Saururus Chinensis (Lour.) Bail)열수추출물의 항암활성 검색을 위하여 여러 암세포에 대한 저해효과와 항산화효과를 측정하고 발암물질인 MNNG를 투여한 동물조직내의 항산화 효소 활성변화에 미치는 영향 등을 살펴보았다. 그 결과 삼백초 열수추출물은 특히 혈액암 세포인 U937에 대해 가장 높은 저해 활성을 나타내었고, 또한 Chinese hamster V79 cell을 이용한 H2O2로 유도된 세포독성에 대한 억제효과도 나타내었다. 그리고 삼백초 열수추출물은 MNNG를 투여한 다음 감소된 glutathione S-transferase와 superoxide dismutase의 활성과 glutathion의 함량을 높여주었다. 이상의 결과로 삼백초 열수추출물은 항산화에 관련하여 발암을 예방하리라 사료된다. Chemoprevetion effects of Saururus Chinensis (Lour.) Bail water extracts on several tumor cells, Chinese hamster V79 cells and antioxidant enzymes in Wistar male rats were investigated. The water extracts of Saururus Chinensis (Lour.) Bail showed a higher cytotoxicity effect on the human histiocytic leukemia cells(U937) and protective effects against the cytotoxicity of H2O2. The glutathione content was decreased by MNNG treatment but was increased by adding Saururus Chinensis (Lour.) Bail water extracts. Also the activities of glutathione S-transferase and superoxide dismutase were increased by the treatment of Saururus Chinensis (Lour.) Bail water extracts more than with MNNG alone. These results suggest that the water extracts of Saururus Chinensis (Lour.) Bail inhibit the growth on U937 and has a protective effect on liver and stomach damage by the activities of free radical generating enzymes.

週齡에 따른 생쥐 Leydig세포의 전자현미경적 구조

서의수,박매자,김덕훈,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.3

주령(週齡)의 증가에 따른 생쥐 Leydig세포의 광학현미경형태와 초미세구조에 관한 변화는 다음과 같다. 1. 생후 1주군에서 미분화된 Leydig세포는 정세관 주위에서 관찰할 수 있었고, 핵은 방추형이며, 핵질과 핵막에는 이염색질이 많이 분포하고, 세포질에는 소포체와 사립체가 조금 분포하나 그 이외의 세포소기관은 거의 없었다. 2. 3주군에서의 Leydig세포는 아직 완전히 분화되지는 않았으나 정세관 주위에서 관찰되었다. 핵은 난형이며, 핵막의 일부 함입과 이염색질의 부착이 있고 세포질에는 잘 발달된 무과립성 내형질망과 membrane whorl이 있으나 그 이외 세포소기관의 발달은 미약하였다. 3. 5주군에서는 완전히 분화한 Leydig세포들의 군집이 정세관 주위에서 관찰되었다. 핵은 거의 원형이며, 핵에는 진염색질이 분포하며, 세포질에는 사립체의 발달이 현저하고, 그 이외의 세포소기관은 약간 증가하였다. 4. 7주군에서는 잘 발달된 정세관의 구조와 잘 분화된 Leydig세포들의 군집을 tubule의 각 공간에서 관찰할 수 있었다. 세포는 핵이 원형으로, 핵막은 뚜렷하고 세포질에는 무과립성 내형질망, 지방소적, 용해소체, 사립체, 당원입자 등이 아주 잘 발달되어 있었다. This study was performed to investigate the ultrastructure of the Leydig cell on aging of the mouse. The results obtained were as follows. One week after birth: The undifferentiated Leydig cells are found around seminiferous tubules. The nucleus showed the fusiform shape. The heterochromatin was found to be adherent to the nuclear membrane and to be dispersed in the nucleoplasm. The smooth endoplasmic reticulum and mitochondria were poorly developed, and the other cell organelles did not appear at this stage. The cytoplasmic vacuolations began to appear. After 3 weeks: Not fully differentiated Leydig cells are present around tubules. The nucleus had oval shape, and some nuclear membrane was caved and adhered to the heterochromatin. The smooth endoplasmic reticulum and membrane was caved and adhered to the heterochromatin. The smooth endoplasmic reticulum and membrane whorl were well developed. But the other cell organelles were poorly developed. After 5 weeks: Clusters of fully differentiated Leydig cells are found around the tubules. The nucleus had round shape, and the nucleoplasm included an euchromatin. Numerous mitochondria were ovserbed at this stage. After 7 weeks: Clusters of fully differentiated Leydig cells are located in the interspace of each tubules. The shape of nucleus was round, and nuclear membrane was prominent. The smooth endoplasmic reticulum, lipid droplets, lysosomal dense body, mitochondria, ribosome and glycogen were increased markedly in number.

가스터빈용 소재 IN 738LC 합금의 미세조직 열화거동

유정훈,주성욱,신기삼,김의현,정진성,장성호,송기욱,하정수 국립7개대학공동논문집간행위원회 2002 공업기술연구 Vol.2 No.-

IN 738LC, used as gas-turbine for power generation, was heat treated at 750℃, 850℃, 950℃ for 1000, 2000, and 4000 hrs. and the microstructural evolution are examined using optical microscope, XRD, SEM/EDS. For comparison, part of gas turbine used for 18439 hours for power generation was also examined. The results showed γ´, the main strengthening elements in this alloy, was about 300 nm and was about 56% in as-cast samples. The volume fraction of γ´peaked at 2000 hours at 750℃ and then decreased at further or longer heat treatment. The average diameter of the γ´ which was about 200 nm increased to about 1㎛. Carbides were formed at dendrite, cell, or grain boundaries which was ascribed to the segregation caused by solute redistribution during solidification. It was found that the at low temperature, M_6C type carbides formed, whereas carbides of M_23C_6 type formed at higher temperature, or at longer degradation.