지리산국립공원 내 산림관리에 관한 전문가 인식 조사

김동현 ( Dong Hyeon Kim ),김의경 ( Eui Gyeong Kim ),박상병 ( Sang Byeong Park ),이정환 ( Jeong Hwan Lee ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.4

This study carried out the recognition of appropriate forest managements and directions in Jirisan National Park. For this purpose, we undertook interview survey about the recognition of forest management in Jirisan National Park from ecological and forest research fields` experts. From the statistical analysis results, it was founded that the difference among respondents` types was detected about recognition of forest management in National park. However, all the respondent types recognized that the forest management in National park is essential. So the forest management in national park by forest experts and relevant institutions are revealed needed.

혈우병 소견을 가진 뇌출혈 환자 치험(治驗) 1례(例)

정인태,서병관,이현종,하지영,강미경,홍장무,백용현,박동석,최도영,Jung, In-tae,Seo, Byung-kwan,Lee, Hyun-jong,Ha, Ji-young,Kang, Mi-kyeong,Hong, Jang-moo,Baek, Yong-hyeon,Park, Dong-suk,Choi, Do-young 대한침구의학회 2003 대한침구의학회지 Vol.20 No.4

Objective : Hemophilia, a genetically determined disorder, is characterized by abnormality of the coagulation mechanism due to functional deficiency of a specific factor, namely VIII or IX. In this study, the effect of Dong-Si acupuncture therapy on the cerebral hemorrhage patient with hemophilia was evaluated. Methods : We treated him with acupuncture by stimulating Dong-Si acupuncture point(Yuk Wan) and assessed the effect by using manual dynamic evaluation of MRC(Medical Research Council) and Modified Barthel Index. Results : After treatment, the bleeding tendency was grossly reduced and bleeding lesion was absorbed gradually. And manual dynamic evaluation of the upper extremity increased from 4 grade to 5 grade. Conclusions : This result suggest that Dong-Si acupuncture therapy on the cerebral hemorrhage patient with hemophilia was effective. And futher studies are required to concretely prove the effectiveness of Dong-Si acupuncture therapy for treating hemorrhagic diseases.

Rifampicin에 의한 것으로 추정되는 위막성 대장염1예

김수현,이은우,정종혁,문승현,김동한,양혁승,오영상,김호동,김도현,박혁,박정환,박경옥,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Pseudomembranous colitis, caused by altering the normal colonic flora and allowing the multiplication of Clostridium difficile, is an deleterious adverse effect of antibiotics. But it is rarely reported by rifampicin. Rifampicin is one of the first line drug in the treatment of tuberculosis and many patients are exposed to its potential adverse effects. We experienced a patient that had abdominal discomfort and hematochezia due to pseudomembranous colitis after receiving antituberculous medication, and which was probably caused by rifampicin. A 82 years old man was admitted with abdominal discomfort and hematochezia for one week. On the past history he had been diagnosed as endobronchial tuberculosis about 4 months ago. Colonoscopy revealed multiple discrete whitish mucosal lesion on rectosigmoid colon, and histologic findings were consistent with pseudomembranous colitis. The antituberculous agents were discontinued and vancomycin was administered. The patient's symptoms were resolved within several days. There was no recurrence after reinstitution of the antituberculous agents excluding rifampicin. We report here on a case of pseudomembranous colitis probably due to rifampicin.

METRx™ 기구를 이용한 후방 경추부 추궁추간공 제거술의 예비 결과

박형천,윤승환,박종운,하윤,현동근,김은영,박현선,임대철 대한척추신경외과학회 2004 Neurospine Vol.1 No.1

Objectives: Posterior cervical microscopic laminoforaminotomy has been a preferred procedure for a posterolateral cervical disc or foraminal stenosis. However, neckpain from wide skin incision and muscle injury are some of disadvantages. The authors performed cervical microscopic laminoforaminotomy with METRx™ tubular dilator system and were compared the results with classical cervical microscopic foraminotomy. Methods: Six patients underwent posterior cervical microscopic laminoforaminotomy using the METRx™ during 6 months. We have selected 10 patients from our institution as a comparison group. Preoperative and postoperative pain score composed of the radicular and neck component was examined and disability score also recorded. Diameters and lengths of laminoforaminotomy on the postoperative CT also were compared with two methods Results: Postoperative pain scores to the radiculopathies were not different but postoperative pain scores to the neckpain on the follow up periods were significantly different. The average vertical and transverse diameters of the laminoforaminotomy defect on the postoperative CT scans were not different between two groups. Average hospital stay of the classical methods was 8.8±7.5 days comparison with 4.5±0.54days of new procedure. Conclusions: Posterior cervical microscopic laminoforaminotomy with METRx™ systems seems to provide similar decompression and clinical results as classical methods compared to classical methods and shows superior results for postoperative neckpain and hospital stays.

Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現

朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.

수산화칼슘 처리된 Porphyromonas endodontalis Lipopolysaccharide가 다형핵백혈구의 IL-1과 TNF-α 생성에 미치는 영향에 관한 연구

박찬제,박동성,유현미,오태석,임성삼 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

Bacterial lipopolysaccharide (LPS) plays a major role in stimulating the synthesis and release of the principal osteoclast-activating cytokines , namely, interleukin 1 and tumor necrosis factor-α from immune cells, Although monocytes/macrophages are the main producers of these cytokines, recent evidence has indicated that polymorphonuclear leukocytes (PMN) have the ability to release IL-1 and TNF-α. Calcium hydroxide has been shown to be an effective medicament in root canal infectionsm, reducing the microbial titre within the canal. It has been proposed that the therapeutic effect of Ca(OH)_2 may also be the result of direct inactivation of LPS. The purpose of this study was to investigate whether treatment of Porphyromanas endodonatalis LPS with calcium hydroxide alters its biological action as neasured by human PMN secretion of IL-1 and TNF-α, and it was compared with Escherichia coli LPS. P.endodonatalis ATCC 35406 was cultured in anaerobic condition, and LPS was extracted using the hot-phenol water extraction method and purified. Purchased E. coli LPS was also purified . 100 ㎍/ml of each LPS in pyrogen free water were incubated with 25mg/ml Ca(OH)_2 at 37℃ for 7 days. The supernatants were subjected to ultrafiltration. And the isolates were lyophilized and weighed. PMNs were obtained from peripheral blood by centrifugation layered over Lymphoprep. The cells were resuspended (4 × 10^6 cells/ml) in RPMI 1640 followed by treatment with various concentrations of LPS (0, 0.1, 1, 10㎍/ml) for 24 hours at 37℃ in 5% CO_2 incubator. The supernatants of cells were collected and the levels of IL-1α, IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. The results were as follows; 1. The levels of IL-1α, IL-1β, TNF-α from PMN treated with each LPS were significantly higher than those released from unstimulated PMN of the control group (p<0.05). 2. The levels of all three cytokines released from PMN stimulated with each calcium hydroxide treated LPS were significantly lower than those released from PMN stimulated with each untreated LPS (p<0.05). while they were not significantly different from those released from unstimulated PMN of the control group (p>0.05). 3. The levels of secretion or all three cytokines were affected in a dose-dependent manner in PMN stimulated with each LPS (p<0.05), but not in PMN stimulated with each calcium hydroxide treated LPS (p>0.05). 4. The levels of all three cytokines released from PMN stimulated with P.endodontalis LPS were significantly lower than those released from PMN stimulated with E. coli LPS (p<0.05).

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

다발성으로 피부절을 수반한 대상포진 뇌수막염 1예

한동철,박종호,신원철,이영배,박현미,신동진 대한노인병학회 2004 대한노인병학회지 Vol.8 No.1

활성탄에 의한 Polysilane의 분자량 조절

천종현,최광진,박동호 인제대학교기초과학연구소 2002 자연과학 Vol.6 No.-

SiC-fiber의 전구체로 쓰이는 polymethylsilane(PMS), polydimethylsilane(PDMS),polyemthylphenylsilane(PMPS)은 Na촉매 하에 dichloro-organosilanes의 탈 염소화 반응으로 합성된다. 유기용매에 잘 녹는 PMPS를 이용하여 온도, 시간, 첨가제의 농도에 따른 수율과 평균분자량을 조사하였으며,고온에서의 잔류량을 확인하였다. PMPS의 합성시간이 증가할수록 수율과 분산도가 증가하였고 평균분자량이 감소하였다. 활성탄을 첨가하여 합성 시 PMPS은 높은 평균 분자량을 가졌다. Na/C=0.05 첨가 시까지 평균 분자량이 높아졌고, 고분자 분산도는 균일했다. Na/C=0.1 첨가 시 분자량과 분산도가 크게 증가했다. PMPS의 고온 잔류량은 활성탄의 첨가량을 많이 하고, 첨가 후 반응 시간을 늘려줄수록 증가하였다. 반응 시 첨가되는 활성탄의 양과 합성시간을 조절함으로 PMPS의 평균 분자량을 조절할 수 있었다. Polymethylsilane(PMS), polydimethylsilane(PDMS),polyemthylphenylsilane(PMPS), which were used as a precursor of SiC-fiber, were synthesized by dehalocoupling were dichloro-organosilanes using Na as a reductant. By using the PMPS which is easily melted at organic solvent, investigated how yield, average molecular weight and remaining quantity in high temperature be influenced by temperature, time and concentration of additive. When reaction time of PMPS became longer, yield and polydispersity were increased and average molecular weight was decreased. synthesis of PMPS with active carborn as an additive showed high average molecular weight was high while polydispersity was regular. In case of Na/C=0.1, average molecular weight and polydispersity was remarkably increased. Residual quantity in high temperature of PMPS was increased in lots of active carborn and long reaction time. Average molecular weight of PMPS can be controlled by reaction time and amount of active carborn added in polymerization.