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Research on the Surface Defect Detection of Magnetic Sheet for Industrial Manufacture
Wenbo Zhang,Rongwei Duan,Yongxin Feng,Deyu Zhang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.1
The research of defect detection technology of magnetic sheet surface has important significance for improvement of production efficiency and product quality in enterprise. A single threshold method is used for binary segmentation of magnetic sheet image in this paper, then the template localization algorithm is used for template position of magnetic sheet image after binary segmentation, the magnetic sheet image with different position should be transformed into a unified coordinate system for detecting, then least square method is used for fitting straight line and fillet edge, then the calculated values are compared with standard value to determine whether are knock edges. Further, the appropriate grayscale threshold is set by the pitting and scratches characteristics of magnetic sheet image, the magnetic sheet image should be segmented according to the determined threshold value, if the defect exists, the defect area or length will be calculated, then the result is compared with standard values to determine whether is pitting or scratches. The final tests of technology in this paper show that the detection rate of knock edge and the detection rate of pitting or scratches are both acceptable.
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Lewen Zhang,Deyu Guo,Jing Wu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8
The stability of the rocky slope is a systematic problem under the combined action of many complex and changeable factors. In this paper, a comprehensive evaluation model based on VIKOR and binary semantics is proposed, and the stability of the rocky slope is predicted and evaluated. Fristly, according to the damage mechanism and the failure process of the slope rock mass, eight influence factors, such as rock cohesion, internal friction angle, and seismic intensity, are selected as the evaluation indexes for the rocky slope stability forecast. The rocky slope stability evaluation index system has been established. Secondly, a stability forecast model of the rocky slope based on VIKOR and the binary semantics method is proposed. The visualization window and the software system of the prediction model are realized by the Python 3.5 software. Finally, the software is applied to the stability evaluation of the rocky slopes, such as Jinping I Hydropower Station and Longtan Hydropower Station. The result shows that: 1) The visual window and the software operating system of the rocky slope stability forecast model, as well as the evaluation index system replacement button are realized, which improve the calculation efficiency of the sample data and the practicability and applicability of the software system. 2) The calculation results of the rocky slope stability prediction model based on VIKOR and the binary semantics method proposed in this paper are more consistent with the actual engineering situation, and more accurate than the prediction results obtained by other evaluation methods. 3) The proposed software system of the rocky slope stability is also suitable for other geotechnical engineering such as tunnels and coal mines, and which has a good prospect of the engineering application.
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Man Zhang,Deyue Yu,Kai Li,Jianyu Liu 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
Plant 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) were considered to be encoded by single copy. In this study, we successfully isolated two DXR isogenes, designated GmDXR1 and GmDXR2, from soybean. The multicopy nature of DXRs in soybean was supported by Southern blot. Alignment of the two GmDXRs showed the presence of the N-terminal transit peptide for plastids, the conserved cleavage site, prolinerich region, and the NADPH-binding motif. Phylogenetic analysis revealed GmDXR1 and GmDXR2 belonged to different branches of angiosperm DXRs clusters. Expression pattern analysis indicated that GmDXR1 was expressed in all analyzed organs except the pod walls, with the highest level in seeds, whereas no message was detected for GmDXR2. In response to heat stress, GmDXR1 showed declined transcript levels in the experiments; in contrast,GmDXR2 was highly responsive, with mRNA accumulation peaking at 6 h after treatment. We also demonstrated that both GmDXRs were localized in chloroplasts. Overexpression of GmDXRs induced increased contents of various isoprenoids (chlorophyll, carotenoids, and gibberellins),but with reduced level of ABA, indicating that GmDXRs participate in the control of differential isoprenoid biosynthesis of the MEP pathway. This work provides a new insight into the wide presence of multicopies of DXR enzyme in plants.
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Ying Liang,Deyu Kong,Yi Zhang,Siqi Li,Yan Li,Anuradha Ramamoorthy,Junfeng Ma 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.2
Thyroid cancer is the most important malignant tumor reported in human populations where, its treatment remains undeveloped. Fisetin, a plant flavonoid exhibits several pharmacological properties like antioxidant, antiinflammatory, and anticancer function. In the existing study, we assessed fisetin mediated cytotoxic effects and action potential of fisetin on cell proliferation in TPC-1 human cancer cells. Also, examined the apoptosis in TPC-1 cells by reactive oxygen species (ROS) generation, the mitochondrial membrane potential (MMP) and apoptotic morphological changes through AO/EtBr staining. Additionally, we analyzed the effects of fisetin through ELISA analysis to evaluate the caspases expression and studied JAK 1 and STAT3 signaling molecule in TPC1 cells. Our results demonstrated that fisetin stimulated apoptosis, which confirmed through reduced cell viability, improved ROS generation, altered MMP and cell cycle phases in TPC-1 cells. Further, the fisetin up-regulated the expression of caspase (3, 8, and 9) expressions in TPC-1 cells. Also, we observed the fisetin down-regulated the JAK 1 and STAT3 expression in TPC1 cells. Thus, the fisetin induced apoptosis in TPC-1 cells by the induction of oxidative damage and enhanced caspases expression by down-regulating JAK 1 and STAT3 signaling molecules. Hence, the fisetin would be considered as a beneficial therapeutic agent for the thyroid cancer treatment.
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Vibration control performance of particle tuned mass inerter system
Zheng Lu,Deyu Yan,Chaojie Zhou,Ruifu Zhang 국제구조공학회 2024 Structural Engineering and Mechanics, An Int'l Jou Vol.89 No.4
To improve the vibration control performance and applicability of traditional particle tuned mass damper (PTMD) and realize the significant characteristic of lightweight design, this study proposes a novel particle tuned mass inerter system (PTMIS) by introducing inerter system (IS) to the PTMD. In the study, the motion equation of single degree of freedom (SDOF) structure attached with PTMIS is established first, then the variation law of the system’s vibration reduction performance (VRP) is discussed through parameter analysis, and it is compared with the PTMD to analyze its VRP advantages. Finally, its vibration reduction (VR) mechanism from the perspective of core control force and energy analysis is explored, and its cavity relative displacement from the application perspective is analyzed. The results show that the PTMIS can remarkably improve the vibration control effectiveness of the PTMD. The reason is that the inerter can store energy and transfer the energy to the cavity and particles, which further stimulates the interaction between the two parts, thereby improving the nonlinear energy consumption effectiveness. Also, the IS can amplify the damping element’s energy dissipation efficiency. In addition, the PTMIS can effectively reduce the working stroke of the PTMD, and through the analysis of the lightweight characteristics of the PTMIS, it is found that its lightweight advantage can reach nearly 100%.
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Cloning and functional analysis of two GmDeg genes in soybean [Glycine max (L.) Merr.]
Xing Kong,Jingyao Zhang,Deyue Yu,Jun-Yi Gai,Shouping Yang 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1
Although light is the ultimate substrate in photosynthesis, strong light can also be harmful and lead to photoinhibition. The DEG proteases play important roles in the degradation of misfolded and damaged proteins. In this study, two photoinhibition-related genes from soybean [Glycine max (L.) Merr.], GmDeg1 and GmDeg2, were cloned. Bioinformatics analysis indicated that these two proteases both contain a PDZ domain and are serine proteases. The expression levels of GmDeg1 and GmDeg2 increased significantly after 12 h of photooxidation treatment, indicating that GmDeg1 and GmDeg2 might play protective roles under strong light conditions. In in vitro proteolytic degradation assays, recombinant GmDeg1 and GmDeg2 demonstrated biological activities at temperatures ranging from 20°C to 60°C and at pH 5.0 to 8.0. By contrast, the proteases showed no proteolytic effect in the presence of a serine protease inhibitor. Taken together, these results provided strong evidence that GmDeg1 and GmDeg2 are serine proteases that could degrade the model substrate in vitro, indicating that they might degrade damaged D1 protein and other mis-folded proteins in vivo. Furthermore, GmDeg1 and GmDeg2 were transformed into Arabidopsis thaliana to obtain transgenic plants. Leaves from the transgenic and wild-type plants were subjected to strong light conditions in vitro, and the PSII photochemical efficiency (Fv/Fm) was measured. The Fv/Fm of the transgenic plants was significantly higher than that of the wild-type plants at most time points. These results imply that GmDeg1 and GmDeg2 would have similar functions to Arabidopsis AtDeg1, thus accelerating the recovery of PSII photochemical efficiency.
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Qian Wu,Hui Wang,Juanjuan Wu,Dagang Wang,Yongli Wang,Lei Zhang,Zhiping Huang,Deyue Yu 한국식물학회 2015 Journal of Plant Biology Vol.58 No.4
The evaluation and use of endogenous soybean genes is an effective strategy to minimize the yield losses caused by insects. Allene oxide cyclase (AOC) catalyzes the most important step in the biosynthesis of jasmonate (JA), which plays a crucial role in plant defense against insects. In this study, the role of GmAOC3 in plant insect resistance was evaluated. Real-time PCR results indicate that GmAOC3 was uniquely and rapidly activated and attained peak expression in leaves after attack by the common cutworm (CCW). In insect bioassays, transgenic lines overexpressing GmAOC3 were significantly less damaged than wild-type plants, and the relative growth rate of CCW fed with leaves from transgenic lines was significantly lower than that of CCW fed with leaves from wild-type plants. Electron microscopy revealed that the density of leaf trichomes in transgenic lines overexpressing GmAOC3 was greater than that in wild-type tobacco. Several physiological and morphological indicators, including JA, phenolic content and the relative expression levels of the putrescine N-methyltransferase (PMT) and proteinase inhibitor (PI) genes, phenylalanine ammonia lyase (PAL) activity and volatile substances, increased in the transgenic plants overexpressing GmAOC3. Our findings indicate that GmAOC3 plays an important role in soybean resistance to CCW and can be used as a resource for plant breeding.
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