Enhancing immune responses to inactivated foot-and-mouth virus vaccine by a polysaccharide adjuvant of aqueous extracts from Artemisia rupestris L.

Danyang Wang,Yu Yang,Jinyu Li,Bin Wang,Ailian Zhang 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.3

Background: New-generation adjuvants for foot-and-mouth disease virus (FMDV) vaccines can improve the efficacy of existing vaccines. Chinese medicinal herb polysaccharide possesses better promoting effects. Objectives: In this study, the aqueous extract from Artemisia rupestris L. (AEAR), an immunoregulatory crude polysaccharide, was utilized as the adjuvant of inactivated FMDV vaccine to explore their immune regulation roles. Methods: The mice in each group were subcutaneously injected with different vaccine formulations containing inactivated FMDV antigen adjuvanted with three doses (low, medium, and high) of AEAR or AEAR with ISA-206 adjuvant for 2 times respectively in 1 and 14 days. The variations of antibody level, lymphocyte count, and cytokine secretion in 14 to 42 days after first vaccination were monitored. Then cytotoxic T lymphocyte (CTL) response and antibody duration were measured after the second vaccination. Results: AEAR significantly induced FMDV-specific antibody titers and lymphocyte activation. AEAR at a medium dose stimulated Th1/Th2-type response through interleukin-4 and interferon-γ secreted by CD4+ T cells. Effective T lymphocyte counts were significantly elevated by AEAR. Importantly, the efficient CTL response was remarkably provoked by AEAR. Furthermore, AEAR at a low dose and ISA-206 adjuvant also synergistically promoted immune responses more significantly in immunized mice than those injected with only ISA-206 adjuvant and the stable antibody duration without body weight loss was 6 months. Conclusions: These findings suggested that AEAR had potential utility as a polysaccharide adjuvant for FMDV vaccines.

Soft‑template‑assisted synthesis of N‑doping layered CoS2 nanoparticles as an advanced anode for sodium‑ion batteries

Danyang Han,Guo Yu,An Liu,Gangyong Li,Wei Wang,Binhong He,Zhaohui Hou,Hong Yin 한국탄소학회 2023 Carbon Letters Vol.33 No.6

As a promising anode for sodium-ion batteries (SIBs), cobalt sulfide ( CoS2) has attracted extensive attention due to its high theoretical capacity, easy preparation, and superior electrochemical activity. However, its intrinsic low conductivity and large volume expansion result in poor cycling ability. Herein, nitrogen-doped carbon-coated CoS2 nanoparticles (N–C@ CoS2) were prepared by a C3N4 soft-template-assisted method. Carbon coating improves the conductivity and prevents the aggregation of CoS2 nanoparticles. In addition, the C3N4 template provides a porous graphene-like structure as a conductive framework, affording a fast and constant transport path for electrons and void space for buffering the volume change of CoS2 nanoparticles. Benefitting from the superiorities, the Na-storage properties of the N–C@CoS2 electrode are remarkably boosted. The advanced anode delivers a long-term capacity of 376.27 mAh g? 1 at 0.1 A g? 1 after 500 cycles. This method can also apply to preparing other metal sulfide materials for SIBs and provides the relevant experimental basis for the further development of energy storage materials.

Viscous dissipation influencing viscosity of polymer melt in micro channels

Bin Xu,Minjie Wang,Tongmin Yu,Danyang Zhao 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.7

Determination of melt rheological behavior within micro-structured geometry is very important for the accurate simulation modeling of micro-molding. Yet studies on the rheological behavior of polymer melts, flowing through micro channels, are complicated due to a large number of factors affecting the melt viscosity. One factor, viscous dissipation, is investigated in the current work through a novel experimental technique to determine the viscous dissipation of a polymer melt flowing through several micro channels with identical aspect ratio. Relative tests are conducted with the melt of high density polyethylene (HDPE) at different temperatures being extruded through the capillary dies with diameters 1000μm, 500μm and 350μm, respectively. It was found that the temperature rise due to viscous dissipation decreases significantly with the reduction of the characteristic size of micro channel at the same shear rate. In addition, based on the suggested model of radial temperature distribution, the influence of viscous heating on the melt viscosity is investigated. The results indicate that viscous dissipation does not play a significant role.

Comparison of Acyrthosiphon pisum probing behaviors on different alfalfa cultivars

Yu Liangbin,Cui Jin,Wang Danyang,Zhang Quanyi,Xu Linbo 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1

Acyrthosiphon pisum (Harris) is a major pest of alfalfa worldwide. Here, we evaluated the resistance of eight alfalfa cultivars to A. pisum to identify resistant cultivars. The Giga-8 DC-EPG technique was used to record differences in electrical waveform patterns of probing behavior to identify cultivars with higher resistance. The frequency and average duration of EPG waveforms significantly differed among the cultivars, with eight docu mented waveforms (np, C, pd, E1, E2, sE2, F, and G). The longer duration of np, C, F and E1 waveforms reflected stronger resistance, whereas longer sE2 waveforms reflected weaker resistance. The resistance-related wave forms, np and C, occurred most frequently with Sardi 7, the time of the first E waveforms were the latest, duration of G waveforms and F waveforms were the longest, the feeding waveforms E2 and sE2 were the shortest, the time of reaching phloem for feeding was the latest, with notable resistance in leaf epidermis, mesophyll and phloem. The number of C waveforms occurring with Gibraltar were the least, duration of np waveforms was short, feeding waveforms were long, time to reach the phloem for feeding was early, and the resistance was not pronounced in various tissues. After comprehensive evaluation, the resistance levels of 8 alfalfa cultivars were as follows: High resistance, Sardi 7; Resistance, Zhongcao No. 3, Derby, WL319HQ; Low resistance, Golden Empress, Algonquin, Zhaodong; Susceptible, Gibraltar. In conclusion, evaluation of cultivar resistance to aphids can provide baseline data for selecting and planting cultivars with high local resistance

Research on a Point Cloud Registration Method of Mobile Laser Scanning and Terrestrial Laser Scanning

Bori Cong,Qingying Li,Rufei Liu,Fei Wang,Danyang Zhu,Jiben Yang 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.12

Mobile laser scanning can quickly and dynamically obtain a wide range of urban scene point clouds. However, due to factors such as occlusion and field of view limitation, it needs to be supplemented by terrestrial laser scanning. The acquisition methods and data quality of mobile point clouds and terrestrial point clouds are quite different, the target of urban scene point clouds is complex and diverse, and the corresponding feature is difficult to extract, so the point cloud fusion is difficult. To this end, a point cloud registration method of mobile and terrestrial scanning based on the target features of artificial ground objects is proposed. Firstly, the data features of mobile laser scanning point clouds and terrestrial laser scanning point clouds are analyzed, and the point clouds are diluted with equal density. Then, the artificial ground objects are extracted as the registration primitives to reduce the scene complexity, and the features of urban scenes and the features of point cloud eigenvalues and principal curvature attributes are analyzed. Combined with the octree voxel index, the multi-scale key point extraction method is constructed to extract the multi-scale key points of registration primitives. Finally, the key point constraint is used to improve the deficiencies of 4PCS (4-Points Congruent Sets) algorithm and ICP (Iterative Closest Point) algorithm to complete the registration of mobile and terrestrial point clouds in different road scenes. Experiments show that the point cloud registration accuracy can reach 2.6 cm, which provides a feasible method for high precision fusion of multi-platform laser point clouds.

Restoration of dysregulated intestinal barrier and inflammatory regulation through synergistically ameliorating hypoxia and scavenging reactive oxygen species using ceria nanozymes in ulcerative colitis

Ying Zhang,Hengyu Lei,Pengchong Wang,Qinyuan Zhou,Jie Yu,Xue Leng,Ruirui Ma,Danyang Wang,Kai Dong,Jianfeng Xing,Yalin Dong 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Reactive oxygen species (ROS) overproduction and excessive hypoxia play pivotal roles in the initiation and progression of ulcerative colitis (UC). Synergistic ROS scavenging and generating O2 could be a promising strategy for UC treatment. Methods Ceria nanozymes (PEG-CNPs) are fabricated using a modified reverse micelle method. We investigate hypoxia attenuating and ROS scavenging of PEG-CNPs in intestinal epithelial cells and RAW 264.7 macrophages and their effects on pro-inflammatory macrophages activation. Subsequently, we investigate the biodistribution, pharmacokinetic properties and long-term toxicity of PEG-CNPs in mice. PEG-CNPs are administered intravenously to mice with 2,4,6-trinitrobenzenesulfonic acid-induced colitis to test their colonic tissue targeting and assess their anti-inflammatory activity and mucosal healing properties in UC. Results PEG-CNPs exhibit multi-enzymatic activity that can scavenge ROS and generate O2, promote intestinal epithelial cell healing and inhibit pro-inflammatory macrophage activation, and have good biocompatibility. After intravenous administration of PEG-CNPs to colitis mice, they can enrich at the site of colonic inflammation, and reduce hypoxia-induced factor-1α expression in intestinal epithelial cells by scavenging ROS to generate O2, thus further promoting disrupted intestinal mucosal barrier restoration. Meanwhile, PEG-CNPs can effectively scavenge ROS in impaired colon tissues and relieve colonic macrophage hypoxia to suppress the pro-inflammatory macrophages activation, thereby preventing UC occurrence and development. Conclusion This study has provided a paradigm to utilize metallic nanozymes, and suggests that further materials engineering investigations could yield a facile method based on the pathological characteristics of UC for clinically managing UC.

A Hydrogel-based First-Aid Tissue Adhesive with Effective Hemostasis and Anti-bacteria for Trauma Emergency Management

Dongjie Zhang,Li Mei,Yuanping Hao,Bingcheng Yi,Jilin Hu,Danyang Wang,Yaodong Zhao,Zhe Wang,Hailin Huang,Yongzhi Xu,Xuyang Deng,Cong Li,Xuewei Li,Qihui Zhou,Yun Lu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Clinical tissue adhesives remain some critical drawbacks for managing emergency injuries, such as inadequate adhesive strength and insufficient anti-infection ability. Herein, a novel, self-healing, and antibacterial carboxymethyl chitosan/polyaldehyde dextran (CMCS/PD) hydrogel is designed as the first-aid tissue adhesive for effective trauma emergency management. Methods We examined the gel-forming time, porosity, self-healing, antibacterial properties, cytotoxicity, adhesive strength, and hemocompatibility. Liver hemorrhage, tail severance, and skin wound infection models of rats are constructed in vivo, respectively. Results Results demonstrate that the CMCS/PD hydrogel has the rapid gel-forming (~ 5 s), good self-healing, and effective antibacterial abilities, and could adhere to tissue firmly (adhesive strength of ~ 10 kPa and burst pressure of 327.5 mmHg) with excellent hemocompatibility and cytocompatibility. This suggests the great prospect of CMCS/ PD hydrogel in acting as a first-aid tissue adhesive for trauma emergency management. The CMCS/PD hydrogel is observed to not only achieve rapid hemostasis for curing liver hemorrhage and tail severance in comparison to commercial hemostatic gel (Surgiflo ®) but also exhibit superior anti-infection for treating acute skin trauma compared with clinical disinfectant gel (Prontosan ®). Conclusions Overall, the CMCS/PD hydrogel offers a promising candidate for first-aid tissue adhesives to manage the trauma emergency. Because of the rapid gel-forming time, it could also be applied as a liquid first-aid bandage for mini-invasive surgical treatment.

Drying features of microwave and far-infrared combination drying on white ginseng slices

Xiaofeng Ning,Yulong Feng,Yuan Juan Gong,Yong Liang Chen,Junwei Qin,Danyang Wang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4

In this study microwave and far-infrared combinationdrying were conducted to investigate the effect ofmicrowave and far-infrared heating mode switching pointwater content (SW), ginseng slice thickness, and far-infrareddrying temperature on drying indicators (surfacecolour difference, ginsenosides content, and surfaceshrinkage rate) and drying efficiency (drying time) duringthe process of drying white ginseng slices. Regardingmicrowave drying, the microwave drying time cannotexceed 150 s, and the ginseng slice water content cannot beless 50%. For the combination drying, SW, far-infrareddrying temperature and slice thickness increased, the colourdifference and surface shrinkage rate first decreasedand then increased, and the content of ginsenosides firstincreased and then decreased. In addition, the combinationdrying showed faster drying rate, higher ginsenosidescontents value, colour difference (DE) value and lowersurface shrinkage rate than single far-infrared drying.

Mimicking synaptic plasticity and learning behaviours in solution processed SnO₂ memristor

Pan, Ying,Wan, Tao,Du, Haiwei,Qu, Bo,Wang, Danyang,Ha, Tae-Jun,Chu, Dewei Elsevier 2018 JOURNAL OF ALLOYS AND COMPOUNDS Vol.757 No.-

<P><B>Abstract</B></P> <P>In this study, a transparent memristor with a configuration of Au/SnO<SUB>2</SUB>/FTO is fabricated by a simple solution process at low temperature and further utilized to mimic biological synapses. A series of significant synaptic functions, including nonlinear transmission characteristics, spike-rate-dependent plasticity (SRDP), short-term plasticity (STP) and long-term plasticity (LTP) are emulated. The transition from short-term to long-term plasticity is also investigated in the device by repeated stimulation. The nonlinear rectification characteristic in the current memristor is attributed to the Schottky barrier at the Au/SnO<SUB>2</SUB> interface. By controlling the oxygen vacancy migration induced under electrical input, the barrier at the interface can be modified, giving rise to the different synaptic functions. These results suggest that the proposed Au/SnO<SUB>2</SUB>/FTO memristor in this study is a promising synaptic device for artificial neural network applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Synaptic electronic based on Au/SnO<SUB>2</SUB>/FTO memristor was fabricated through a simple solution process at low temperature. </LI> <LI> Synaptic plasticity including SRDP, STP, LTP and STP-to-LTP transition were demonstrated in the synaptic device. </LI> <LI> Different synaptic functions were realised by controlling oxygen vacancy migration induced by electrical input. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effects of Flavonoids on Physical and Oxidative Stability of Soybean Oil O/W Emulsions

Dan Yang,Xiang-Yu Wang,이정희 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3

Flavonoids have attracted attention due to pharmacological and antioxidative activities. The effects of flavonoids on the physical and oxidative stabilities of lecithin emulsified soybean oil-in-water (O/W) emulsions were investigated at 25oC during 29 days of storage. Addition of 100 ppm hesperidin, hesperitin, rutin, or quercetin improved the physical stability of O/W emulsions but did not change particle size values, compared to a control with no flavonoids during storage. Quercetin showed the highest antioxidant activity for inhibition of lipid oxidation based on lowered lipid hydroperoxide formation and 2-thiobarbituric acid reactive substances values in emulsions, followed by rutin, hesperitin, and hesperidin. Hesperidin and hesperitin did not affect antioxidative activities in O/W emulsions under metal ion-catalyzed conditions. Addition of hesperidin, hesperitin, rutin, and quercetin to soybean oil O/W emulsions improved the physical and oxidative stability of emulsions lacking added metal ions.