The preliminary study of exosomes derived from thymosin beta 4-treated adipose-derived stem cells in fat grafting

Li Wandi,Yang Yan,Zhang Xiaoyu,Lin Yan,Li Haoran,Yao Yu,Mu Dali 한국유전학회 2023 Genes & Genomics Vol.45 No.4

Background The retention rate in autologous fat grafting is an increasing concern for surgeons and patients. Our previous research verified that thymosin beta 4 (Tβ4) positively affected fat survival, while the mechanism was unknown. The endothelial cells (ECs) and exosomes derived from adipose-derived stem cells (ADSCs) were regarded to play a critical role in fat transplantation. Objective This study aimed to evaluate the effect of exosomes derived from Tβ4-treated ADSCs on EC proliferation and to identify the exosomal microRNA (miRNA) profile compared with the Tβ4-untreated group. Additionally, this research intended to recognize the related molecules and signaling pathways in the Tβ4-treated group with potential roles in fat transplants. Methods ADSCs were collected from patients who underwent liposuction surgery. Depending on whether the medium was supplemented with exogenous Tβ4 or not, exosomes derived from cultured ADSCs were divided into the Tβ4-Exos group and Con-Exos group. Exosome uptake and cell counting kit-8 (CCK-8) assays assessed the influence of Tβ4-Exos on EC proliferation. The exosomal miRNAs of the two groups were analyzed by next-generation sequencing. With the criteria at the |log2 (fold change)| ≥ 1 and p-value < 0.05, up-regulated and down-regulated differentially expressed miRNAs (DEMs) were obtained. Prediction databases were used to predict the downstream mRNAs for DEMs. And then, overlapping genes for the up-regulated DEMs and the down-regulated were screened out, followed by enrichment analysis, protein–protein interaction network construction, and the gene cluster and hub gene identification. Results ADSCs were obtained from four female patients. The exosome uptake and CCK-8 assays showed that the Tβ4-Exos could increase cell growth rate compared with the control group (DMEM-H + PBS). In Tβ4-Exos and Con-Exos groups, 2651 exosomal miRNAs were recognized, with 80 up-regulated and 99 down-regulated DEMs according to the criteria. After the prediction, 621 overlapping genes for the up-regulated and 572 for the down-regulated DEMs were screened. The subsequent bioinformatics analysis found specific molecules and pathways related to the positive effect on fat survival. Conclusions The exosomes derived from Tβ4-treated ADSCs probably positively affect EC proliferation. Compared with the Con-Exos group, several exosomal DEMs, genes, and pathways were distinguished. These findings of this exploratory study provide the potential direction for future in-depth research on fat grafting.

Simulation Research on Control System of Main Converter Unit

Li Xingyu,Zheng Zhongxiang,Wu Dali 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10

The main converter unit needs to provide high-quality AC and DC power for the ship AC/DC hybrid power system. The reliability of its control system is very important. In order to study and optimize the control system of the converter unit, it is very meaningful to model and simulate it. The working characteristics and operation requirements of the converter unit in the ship hybrid power system are introduced. According to the control system structure, the corresponding simulation model is built. The simulation results meet the unit operation requirements and provide technical support for optimizing the converter unit control system.

Asian Germplasm Resources of Yulania Spach (Magnoliaceae)

Dali Fu,Fangdong Li,Donglin Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

EMG-Based Continuous and Simultaneous Estimation of Arm Kinematics in Able-Bodied Individuals and Stroke Survivors

Liu, Jie,Kang, Sang Hoon,Xu, Dali,Ren, Yupeng,Lee, Song Joo,Zhang, Li-Qun Frontiers Media S.A. 2017 Frontiers in neuroscience Vol.11 No.-

<P>Among the potential biological signals for human-machine interactions (brain, nerve, and muscle signals), electromyography (EMG) widely used in clinical setting can be obtained non-invasively as motor commands to control movements. The aim of this study was to develop a model for continuous and simultaneous decoding of multi-joint dynamic arm movements based on multi-channel surface EMG signals crossing the joints, leading to application of myoelectrically controlled exoskeleton robots for upper-limb rehabilitation. Twenty subjects were recruited for this study including 10 stroke subjects and 10 able-bodied subjects. The subjects performed free arm reaching movements in the horizontal plane with an exoskeleton robot. The shoulder, elbow and wrist movements and surface EMG signals from six muscles crossing the three joints were recorded. A non-linear autoregressive exogenous (NARX) model was developed to continuously decode the shoulder, elbow and wrist movements based solely on the EMG signals. The shoulder, elbow and wrist movements were decoded accurately based only on the EMG inputs in all the subjects, with the variance accounted for (VAF) > 98% for all three joints. The proposed approach is capable of simultaneously and continuously decoding multi-joint movements of the human arm by taking into account the non-linear mappings between the muscle EMGs and joint movements, which may provide less effortful control of robotic exoskeletons for rehabilitation training of individuals with neurological disorders and arm impairment.</P>

Preparation of b-Ca3(PO4)2 bioceramic powderfrom calcium carbonate and phosphoric acid q

Yun Zhang,Guangfu Yin,Shifu Zhu,Dali Zhou,Yuehua Wang,Yong Li,Lin Luo 한국물리학회 2005 Current Applied Physics Vol.5 No.5

When preparing b-tricalcium phosphate (b-TCP) with Ca(NO3)2 or Ca(OH)2 as the raw materials, because of the problem ofltering, it is dicult to industrialize traditional methods. In this paper,b-TCP was prepared with calcium carbonate and phospho-ric acid. According to XRD spectrum, the precursor of this system is octacalcium phosphate (OCP) [Ca8H2(PO4)6Æ5H2O] instead ofnon-stoichiometric hydroxyapatite (HA) obtained from traditional methods, and thermodynamics mechanism of the reaction wasalso studied. The crystalb-TCP powders were obtained after the precursor had been calcined at 8501150.C for 2 h. SEM imagesshowed that hardening could eliminate the reunion to a great extent, and obtain dispersiveb-TCP powders with a particle diameterbetween 0.5 and 2.0l m. The main advantages of this process over traditional methods are the fast reaction rate, the easy ltering,the ne particle size and the minimum impurity..

A Novel Human BTB-kelch Protein KLHL31, Strongly Expressed in Muscle and Heart, Inhibits Transcriptional Activities of TRE and SRE

Weishi Yu,Yuequn Wang,Yongqing Li,Yun Deng,Zequn Wang,Wuzhou Yuan,Dali Li,Chuanbing Zhu,Xueying Zhao,Xiaoyang Mo,Wen Huang,Na Luo,Yan Yan,Karen Ocorr,Rolf Bodmer,Xiushan Wu 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.5

The Bric-a-brac, Tramtrack, Broad-complex (BTB) domain is a protein-protein interaction domain that is found in many zinc finger transcription factors. BTB containing proteins play important roles in a variety of cellular functions including regulation of transcription, regulation of the cytoskeleton, protein ubiquitination, angiogenesis, and apoptosis. Here, we report the cloning and characterization of a novel human gene, KLHL31, from a human embryonic heart cDNA library. The cDNA of KLHL31 is 5743 bp long, encoding a protein product of 634 amino acids containing a BTB domain. The protein is highly conserved across different species. Western blot analysis indicates that the KLHL31 protein is abundantly expressed in both embryonic skeletal and heart tissue. In COS-7 cells, KLHL31 proteins are localized to both the nucleus and the cytoplasm. In primary cultures of nascent mouse cardiomyocytes, the majority of endogenous KLHL31 proteins are localized to the cytoplasm. KLHL31 acts as a transcription repressor when fused to GAL4 DNA-binding domain and deletion analysis indicates that the BTB domain is the main region responsible for this repression. Overexpression of KLHL31 in COS-7 cells inhibits the transcriptional activities of both the TPA-response element (TRE) and serum response element (SRE). KLHL31 also significantly reduces JNK activation leading to decreased phosphorylation and protein levels of the JNK target c-Jun in both COS-7 and Hela cells. These results suggest that KLHL31 protein may act as a new transcriptional repressor in MAPK/JNK signaling pathway to regulate cellular functions.

Numerical modeling and global performance analysis of a 15-MW Semisubmersible Floating Offshore Wind Turbine (FOWT)

Da Li,Ikjae Lee,Cong Yi,Wei Gao,Chunhui Song,Shenglei Fu,Moohyun Kim,Alex Ran,Tuanjie Liu Techno-Press 2023 Ocean systems engineering Vol.13 No.3

The global performance of a 15 MW floating offshore wind turbine, a newly designed semisubmersible floating foundation with multiple heave plates by CNOOC, is investigated with two independent turbine-floater-mooring coupled dynamic analysis programs CHARM3D-FAST and OrcaFlex. The semisubmersible platform hosts IEA 15 MW reference wind turbine modulated for VolturnUS-S and hybrid type (chain-wire-chain with clumps) 3×2 mooring lines targeting the water depth of 100 m. The numerical free-decay simulation results are compared with physical experiments with 1:64 scaled model in 3D wave basin, from which appropriate drag coefficients for heave plates were estimated. The tuned numerical simulation tools were then used for the feasibility and global performance analysis of the FOWT considering the 50-yr-storm condition and maximum operational condition. The effect of tower flexibility was investigated by comparing tower-base fore-aft bending moment and nacelle translational accelerations. It is found that the tower-base bending moment and nacelle accelerations can be appreciably increased due to the tower flexibility.

A New Method for Piping Risk Evaluation on Unconfined Aquifers under Dewatering of Deep Foundation Pits

Da Li,Jianjun Ma,Chaosheng Wang,Xiaojuan Gao,Mingjing Fang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.8

This paper presents a new method to evaluate the piping risk of unconfined sandy cobble aquifers during the dewatering of deep foundation pits. Firstly, the possibility of piping is judged according to the particle size distribution and pore diameter of the sandy cobble stratum. Secondly, the critical hydraulic gradients of particles with different sizes are calculated according to the pipe flow model. Thirdly, the hydraulic gradient around the foundation pit during the dewatering process is calculated by numerical method. Then, combined with the distribution of hydraulic gradient around the foundation pit and the critical hydraulic gradients of particles with different sizes, the range of moveable particles with different sizes in the surrounding stratum is evaluated. Finally, according to the sandy cobble aquifers conditions and the influence of particle movement on the stratum, the level of piping risk is determined. This method was successfully applied to evaluate the piping risk of Tawan Station in Luoyang Metro Line No.1, and according to the risk evaluation the pensile curtain was recommended for the retaining structure in this case. From the satisfied applications, this method is a reference for the evaluation of piping risk and selection of retaining structure when considering the impact of the dewatering in deep foundation pit with unconfined aquifers on the surrounding stratum.

THE CONTROLLED SYNTHESIS AND STERILIZATION PERFORMANCE OF Ag/Au NANOCOMPOSITE CHAINS

DA LI,HONG-ZHEN XIE,MIN ZHANG,JIN-KU LIU,XIAO-HONG YANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.1

The controlled synthesis of one-dimensional Ag/Au nanocomposite chains with a length of several micrometers (up to 3 μm) was described in this paper. In this synthesis method, the artificial active membrane of celloidin is used and N2H4 ⋅ H2O acts as the reductive agent. The morphologies, structures and optical properties of the product were researched by TEM, SEM, EDS, XRD, and UV-Vis, etc. The synthesis mechanism of product was also postulated. Experiments proved that the synergic sterilization performances of Ag/Au nanocomposite chains were stronger than Ag/Au nanocomposite particles.