CdTe 감마선 검출소자의 Schottky Contact에 관한 연구

장충근,윤만영,유중열,김영전,황선태 충남대학교 기초과학연구소 1989 연구논문집 Vol.9 No.-

To obtain the elementary data about CdTe Schottky diode, we made Schottky Contact by electroless deposition method after growing the CdTe single crystal which has zinc blend structure with CdTe powder (4N). From the result of the experiments on the Schottky diode, we found that the reverse current in N-type Schottky diode is less than that in P-type but barrier height is higher in N-type. And as the deposition time(reaction time) of Schottky Contact becomes longer or the reaction temperature higher, the barrier height and contact resistance are decreased.

CdTe 감마선 검출소자의 Schottky Contact에 관한 연구

장충근,윤만영,유중열,김영전,황선태 충남대학교 자연과학연구소 1989 忠南科學硏究誌 Vol.16 No.1

To obtain the elementary data about CdTe Schottky diode, we made Schottky Contact by electroless deposition method after growing the CdTe single crystal which has zinc blend structure with CdTe powder(4N). From the result of the experiments on the Schottky diode, we found that the reverse current in N-type Schottky diode is less than that in P-type but barrier is higher in N-type. And as the deposition time(reaction time) of Schottky Contact becomes longer or the reaction temperature higher, the barrier height and contact resistance are decreased.

몰리브덴 퍼어말로이의 자기저항 효과에 관한 연구

장충근,유중렬,송재용,윤만영,박재형,손대락 경북대학교 센서기술연구소 1991 센서技術學術大會論文集 Vol.2 No.1

자기저항센서를 제작하기 위하여 Mo-permalloy(Ni:79.43%, Fe:14.47%, Mo:5.7%, Mn:0.38%, C:0.013%)를 슬라이드 그라스에 진공증착하여 sensor element를 제작한 후 포화자속밀도(B_(s)), 보자력(H_(c)), 자기이방성상수(K//), 자기저항변화율(ΔR/R)을 조사하였다. 진공증착된 permalloy 박막의 포화자속밀도는 0.746T 이었으며, 자화주파수 1kHz에서 보자력은 2.90A/cm(//),1.98A/cm(⊥) 이었고, 열처리 후에 1.79A/cm(//). 1.37A/cm(⊥)로 감소되었다. 한편 이 소자의 자기이방성상수는 1.38X10^(4)erg/cm^(3) 이었으며 5x1^(0-3)T의 자장변화에 대만 저항변화율(ΔR/R)은 2.0% 이었다. 앞으로 이 소자의 온도특성, 잡음특성 및 내열성 등을 조사하고자 한다. To fabricate magnetoresistive sensor, Mo-permalloy(Ni:79.43%, Fe:14.47%, Mo:5.7%, Mn:0.38%, C:0.013%) was evaporated on the slide glass. Saturation magnetic induction(B_(s)), coercive field strength(H_(a)), magnetic anisotropy constant(K), and magnetoresistance were measured for the fabricated samples. The evaporated samples show that saturation magnetic induction was 0.746T, and coercive field strengthes were 2,90A/cm(//) and 1.98A/cm(⊥), and this values were reduced to 1.79A/cm(//) and 1.37A/cm(⊥) respectively after annealing. For the measurement of coercive field strength, magnetizing frequency of 1kHz was used. For the fabricated sensor element, magnetic anisotropic constant was 1.38X10^(4)erg/cm^(3) and magneto-resistance change(ΔR/R) was 2.0% under the magnetic field change of 5X10^(-3)T, We will continue study on the characteristics for the temperature, heat resistance, and noise.

RFLP 법을 이용한 종에 따른 鹿茸類 한약재의 유전자 감식 연구

崔湖榮,鄭裕憲,鄭宗云,金圭烈 대한본초학회 2000 大韓本草學會誌 Vol.15 No.2

Cornu Cervi Pantoctricchum is a hairy, dried, non-ossific young horn of a stag on Cervus nippon Temminck or C. elaphus L (family Cervidae) and a popular crude drug used in Korea to reinforce the kidney-yang, to tonify the vital essence and blood, and to strengthen the bones and muscles. The present study is designed to make a DNA marker for breed identification of Cornu Cervi Pantotricshum families and later to establish a gene discrimination. In this experiment some genomic DNA were extracted fine from dried samples, most of them were extracted damaged a lot. In RFLP analysis using restriction enzyme Hinf I, Hae Ⅲ, Hha I and Msp I can be used to distinguish the breed of Cornu Cervi Pantotrichum. Therefore, these polymorphic bands by RFLP can be used in breed identification of deer and Cervi Cornu Pantoctrichum now in use.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

Molecular Characterization of crp,the Cyclic AMP Receptor Protein Gene of Serratia marcescens KTCC 2172

Yoo, Ju Soon,Kim, Hae Sun,Chung, Soo Yeol,Choi, Yong Lark 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.5

Several clones obtained from Serratia marcescens stimulated E. coli TP2139 (Δlac, Δcrp) cells to use maltose as a carbon source. The crp gene clone, pCKB12, was confirmed to stimulate the β-galactosidase activity, by Southern hybridization [31]. The nucleotide sequence of the crp region consisting of 1,979bp was determined. The sequencing of the fragment led to the identification of two open reading frames: One of these, the crp gene, encoded 210 amino acids and the other encoded a truncated protein. The S. marcescens and E. coli crp genes showed a higher degree of divergence in their nucleotide sequence with 120 changes, however, the corresponding amino acid sequences showed only two amino acid differences. Yet, an analysis of the amino acid divergence revealed that the catabolite gene activator protein, the crp gene product, was the most conserved protein observed so far. Using a crp-lac protein fusion, it was demonstrated that S. marcescens CRP could repress its own expression, probably via a mechanism similar to that previously described for the E. coli crp gene.

재배방법이 다른 미나리의 성분 특성

정희종,이홍렬,유맹자 한국식생활문화학회 2001 韓國食生活文化學會誌 Vol.16 No.3

Chemical properties of the different parts of watercress(Oenanthe javanica D.C.) grown and harvested from the culture fields under different cultivating methods were studied. In proximate analyses of watercress in parts, moisture contained more in stem than in root or in leaf, but crude protein and crude lipid contents were lower in stem than in root or in leaf. Crude ash in root contained up to about double amount in leaf or stem. Major mineral elements detected in watercress were Fe, Mg, Ca, and K, and their contents in root was higher than those in stem or leaf. Especially, Fe in root was significantly higher than that in stem or in leaf. Total free sugar composed mainly with fructose and glucose, was the highest in watercress from Hwasoon and followed the watercress from Jeonju and Donggok in order. Free sugar content was highest in leaf and the lowest in root. Major water-soluble vitamins were vitamin C, thiamin and biotin and the content of vitamin C was higher than others. These vitamins contained more in leaf than in stem or root. Niacin contained 6.09 ㎎/100 g in leaf of watercress from Hwasoon, which was much higher than others, but it was not detected in stem of watercress from Jeonju. Organic acids detected were oxalic acid, citric acid and malic acid and other 12 organic acids were not detected. In fatty acid composition, there were significant differences among watercresses from different parts and different culture fields. Linoleic acid, linolenic acid and palmitic acid were major fatty acids contained in watercress and it took about 80% of the total content. Amino acid content in leaf was higher than that in root and in stem. Glutamic acid and proline were major amino acids in stem of watercress from Jeonju and in stem of watercresses from Hwasoon and Donggok, respectively. In leaves of all three watercresses glutamic acid content was the highest.

Molecular Cloning and Characterization of CM Case gene (celC) from Salmonella typhimurium UR

Yoo, Ju-Soon,Jung, Youn-Ju,Chung, Soo-Yeol,Lee, Young-Choon,Choi, Yong-Lark The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.3

The sequence coding for carboxymethylcellulase (CMCase, CelC) was isolated from the DNA of Salmonella typhimurium URl. Comparison between the deduced amino acid sequence of CelC (368 amino acid residues, Molecular mass 41 kDa) and that of the previously published CMCase revealed that this enzyme belongs to the cellulase family 8 and D. The protein was overproduced in Escherichia coli using T7 expression system, and its activity was confirmed by CMC-SDS-PAGE. When the overexpressed CelC protein was tested on cellulose-type substrates, the recombinant protein is able to degrade cellulose-type substrates, such as CM-cellulose, xylan, avicel, lichenan, and laminarin. Optimal temperature and pH for enzyme activity were found to be 50$^{\circ}C$ and pH 6.5, respectively.

Serratia marcescens에서 cAMP receptor protein(CRP)유전자의 클로닝 및 해석

유주순,문종환,정수열,김혜선,최용락 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6

전사조절인자로서 잘 알려져 있는 cAMP receptor protein(CRP)은 cAMP와 DNA에 결합하는 특별한 활성을 가지고 있으며, cAMP-CRP complex를 형성하여 수많은 유전자의 발현조절에 관여한다. 이러한 측면에서 cAMP-CRP의 조절은 어떤 면에서 총체적 조절체계라고까지 한다. 본 연구는 Serratia 균주에서 crp유전자의 분자적 특성 및 cAMP에 의한 발현조절을 받는 분자기구를 해석하고자 유전자를 클로닝하고 발현을 확인하였다. MacConkey배지에서 maltose를 탄소원으로 충분히 이용하지 못하는 대장균 TP2139(△crp,△lac)를 숙주로 이용하고, 염색체DNA를 library로 작성하여 얻은 형질전환체 약 일만개의 콜로니에서 red colony를 나타내는 5종류 의 양성 클론을 얻었다. 이들 클론을 Southern방법으로 확인한 결과 3kb의 단편을 가진 pCKB12클론이 crp유전자를 coding하고 있음을 확인하였다. glpD-lacZ 융합 plasmid인 pLDC6 의 BamHI부위에 pCKB12의 3kb 단편을 삽입시킨 재조합 plasmid pLDC 6-Scrp를 작성하여, 클로닝된 Serratia 의 crp 유전자가 대장균에서 유전자 전사조절에 미치는 영향을 확인한 결과 cAMP-CRP 복합체 형성에 의한 전사조절 기능이 확인되어졌다. One of the better-characterized transcription factor of E. coli is the cAMP receptor protein(CRP) and the CRP binds cAMP and DNA. The cAMP-CRP complex is involved in regulation of many genes at bacteria. The cAMP-CRP regulatory element represents, in some respects, a global regulatory network. The aim of this work was to study the structure and the mechanisms controlling the expression of CRP in Serratia marcescens. We have been get 5 different clones from Serratia which stimulated the cells to use maltose as a sole carbon source in E. coli TP2139. The crp gene clone, pCKB12, was confirmed by Southern hybridization with E. coli crp gene. The location of the crp gene was determined by constructing subclones carrying various portions of pCKB12. To investigate the potential role of CRP in E. cloi, lacZ fused plasmids were constructed and investigated the β-galactosidase activity of the fused plasmid. The Serratia marcescens cAMP receptor protein can substitute the E. coli CRP in transcriptional activation at the lacZ gene. These results suggest that Serratia marcescens cAMP receptor protein complex functions to regulate several promoters in E. coli.

Characterization of the Small Cryptic Plasmid, pGD2, of Klebsiellia sp. KCL-2.

Yoo, Ju-Soon,Kim, Hae-Sun,Chung, Soo-Yeol,Lee, Young-Choon,Cho, Young-Soo,Choi, Yong-Lark Korean Society for Biochemistry and Molecular Biol 2001 Journal of biochemistry and molecular biology Vol.34 No.6

One of the cryptic plasmids from the oil degrading bacterium Klebsiella sp. KCL-2, the small plasmid pGD2, has been identified and characterized. This plasmid has a size of 3.6 kb with unknown functions. We constructed the recombinant plasmid pMGD2. The nucleotide sequences of the plasmid were determined and two open reading frames were detected. ORF1 encodes a replication initiator protein (RepA), which has a high degree of homology with the protein of ColE2 plasmid. The product encoded by ORF2 showed a high similarity with the transposase protein of IS5. IS5 is 1195 by long and contains an inverted terminal repetition of 16 bp with one mismatch. Stem-loop structures in the 5'untranslated region of the repA suggest that a putative gene, incA, is located in a complementary strand to the leader region of the repA mRNA.