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Protective effects of ginsenoside Rg2 and astaxanthin mixture against UVB-induced DNA damage
정유헌,정슬아,최현석,Seungil Ro,이정섭,박종군 한국통합생물학회 2018 Animal cells and systems Vol.22 No.6
Ultraviolet B (UVB) radiation induces skin damage, skin matrix degradation, and wrinkle formation through photochemical reaction and oxidative stress. Therefore, protecting the skin from UVB can prevent skin aging. In this study, we investigated the effects of a mixture (RA) of Rg2, a ginsenoside, and astaxanthin, an antioxidant, on the responses of HaCaT cells exposed to UVB (700 J/m2). The cells were incubated for 24 h after UVB exposure and cell viability was determined by MTT assay. UVB decreased cell viability by 60% compared to that of untreated control cells, whereas RA increased cell viability in a concentration-dependent manner, and this increase was significantly higher than that in the single treatment groups. Further, UVB increased the levels of DNA lesions such as cyclobutane pyrimidine dimer (CPD) and 8-hydroxyguanine (8-OHdG). Conversely, RA decreased both CPD and 8-OHdG levels in a concentration-dependent manner. UVB exposure also increased phosphorylation of ataxia-telangiectasia mutated (ATM) protein kinase and p53 and subsequently increased the levels of GADD45α, p21, and matrix metalloproteinases (MMPs)- 3, -9, and -13. Additionally, UVB exposure decreased the level of COL1A1. However, RA treatment decreased the levels of p-ATM, p-p53, GADD45α, p21, MMP-3, -9, and -13 and increased the level of COL1A1 in a concentration-dependent manner. These results suggest that RA reduces UVBinduced cytotoxicity and genotoxicity through up-regulation of DNA repair via the combined effects of Rg2 and astaxanthin.
Upregulation of autophagy by Ginsenoside Rg2 in MCF-7 cells
정유헌,정슬아,최현석,Seungil Ro,이정섭,박종군 한국통합생물학회 2018 Animal cells and systems Vol.22 No.6
Autophagy is a major intracellular degradation process that plays an important role in cell survival, stress responses, nutrient sensing and development. Our previous studies have shown that Rg2, a triterpenoid saponin contained in ginseng, protects cells against UVB-induced genotoxicity by increasing DNA repair, in possible association with modulation of protein levels involved in p53 pathway. In this study, we determined an upregulation of autophagy by Rg2. Rg2 treatment for 24 h in MCF-7, a breast cancer cell, did not show cytotoxicity up to 200 μM. Rg2 also upregulated the level of p-p53, p-AMPK, p-ACC, Atg-7 and LC3-II and decreased the level of p62 in concentration-dependent manners. We also determined the level of p53, AMPK, p62, Atg-7 and LC3 after UVB exposure and subsequent incubation in growth medium for 24 h. UVB increased the level of p-p53, p-AMPK, p-ACC and decreased the levels of p62, Atg-7 and LC3-II. Interestingly, Rg2 treatment for 24 h after UVB exposure increased the levels of p-p53, p-AMPK, p-ACC, Atg-7 and LC3-II and decreased the level of cyclobutane pyrimidine dimer, a UVB-induced DNA damage in concentration-dependent manners. All these results suggest that Rg2 increased autophagy and decreased UVB-induced DNA damage, in possible association with the modulation of protein levels in p53- and autophagic pathways.
RFLP 법을 이용한 종에 따른 鹿茸類 한약재의 유전자 감식 연구
崔湖榮,鄭裕憲,鄭宗云,金圭烈 대한본초학회 2000 大韓本草學會誌 Vol.15 No.2
Cornu Cervi Pantoctricchum is a hairy, dried, non-ossific young horn of a stag on Cervus nippon Temminck or C. elaphus L (family Cervidae) and a popular crude drug used in Korea to reinforce the kidney-yang, to tonify the vital essence and blood, and to strengthen the bones and muscles. The present study is designed to make a DNA marker for breed identification of Cornu Cervi Pantotricshum families and later to establish a gene discrimination. In this experiment some genomic DNA were extracted fine from dried samples, most of them were extracted damaged a lot. In RFLP analysis using restriction enzyme Hinf I, Hae Ⅲ, Hha I and Msp I can be used to distinguish the breed of Cornu Cervi Pantotrichum. Therefore, these polymorphic bands by RFLP can be used in breed identification of deer and Cervi Cornu Pantoctrichum now in use.
B16F10 세포에서 연두털말 에탄올 추출물의 멜라닌 생성 저해효과
박향하,정유헌,박종군,김수정,정대현,최한길 한국미용학회 2019 한국미용학회지 Vol.25 No.1
To test the possibility of natural cosmetic material, the antioxidant activity using DPPH method and whitening effect for the ethanol extract of a brown alga, Papenfusiella kuromo were examined. In particular whitening effects were estimated by melanin production of B16F10 melanoma cells and confirmed by tyrosinase gene expression in terms of RT-PCR and western bolt analyses. Present results revealed that DPPH radical scavenging activity for P. kuromo extract was about 64% at 50 μg/mL. As compared to IBMX treatment, melanin production of melanoma cells was remarkably inhibited up to 94% at 50 μg/mL of the extract. RT-PCR and western blot analyses showed that the tyrosinase gene expression of the B16F10 cell decreased in a concentration-dependent manner. Especially, antimelanogenic effect at 50 μg/mL of P. kuromo extract was greater than that in the control of Kojic acid treatment, suggesting that the P. kuromo ethanol extract could be a good candidate of whitening agents. Thus, further research on pure chemical isolation of the natural whitening cosmetic materials from P. kuromo extract is required.
자외선 B에 의해 유도되는 DNA 상해에 대한 참갈파래 메탄올 추출물의 보호 효과
정슬아,정유헌,박종군,Jeong, Seula,Chung, Yuheon,Park, Jong Kun 한국식품영양학회 2020 韓國食品營養學會誌 Vol.33 No.3
In this study, we investigated the protective effects of Ulva lactuca methanol extracts against ultraviolet B (UVB)-induced DNA damage in HaCaT cells. First, the contents of general and antioxidative nutrient contents of Ulva lactuca were measured. The moisture, carbohydrate, crude protein, crude fat and ash were 14.01%, 44.80%, 23.19%, 3.10% and 14.90%, respectively. Magnesium that acts as DNA repair enzyme cofactor was the most abundant mineral followed by Ca, P and Fe. The total phenolic and anthocyanoside contents of Ulva lactuca were 2.69 mg/g and 0.13 mg/g, respectively. Cells treated with Ulva lactuca methanol extracts for 24 hours post UVB exposure increased cell viability in a concentration-dependent manner compared to the non-treated control. Also, Ulva lactuca methanol extracts decreased the levels of UVB-induced DNA damage such as cyclobutane pyrimidine dimer and DNA damage response (DDR) proteins such as p-p53 and p21. These results suggest that Ulva lactuca methanol extracts comprising physiological active substances such as Mg, polyphenols and anthocyanosides promote DNA repair by regulating genes related with DDR.
하세은,김민섭,정유헌,박종군 한국통합생물학회 2016 Animal cells and systems Vol.20 No.3
Our previous studies have shown that ginsenoside Rg2 protects the genotoxicity of UVB via earlier upregulation of p53 and GADD45 proteins. In the present study, we investigated the effects of Rg2 on the genotoxicity of UVB in cells transfected with p53 siRNA. The cells transfected with control siRNA, exposed to UVB and then post-incubated with 100 μM Rg2 showed increase in cell viability to about 20%, as compared to no significant increase in cell viability in cells transfected with p53 siRNA. The UVB-induced apoptosis was also decreased by 100 μM Rg2 to about 30% in control siRNA-transfected cells, as compared to only 10% decrease in the apoptosis in p53 siRNA-transfected cells. The p53 and GADD45 protein levels in control siRNA-transfected cells after UVB exposure increased to about 3.5 and 2.7 fold, respectively, as compared to the nontreated control. The increased p53 and GADD45 protein levels were downregulated by Rg2 in an Rg2 concentration-dependent manner. However, the protective effects of Rg2 were not shown in p53 siRNA-transfected cells. All these results suggest that Rg2 protects cells against UVB-induced genotoxicity by increasing DNA repair, in possible association with modulation of protein levels involved in p53 signaling pathway.
DNA 사슬 종결형 항암제인 플루다라빈에 의해 유도된 세포독성에 대한 아로니아-홍삼 에탄올 혼합 추출물의 효과
김민섭 ( Min Seob Kim ),정유헌 ( You Heon Chung ),오홍근 ( Hong Geun Oh ),박종군 ( Jong Kun Park ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.4
Fludarabine, a chain terminating anti-cancer drug, is a purine analogue that causes DNA strand breaks in normal cells. In this study, we determined if A. melanocarpa and Korean red ginseng extract mixture reduce cytotoxicity of fludarabine. Treatment of HaCaT cells with 10 μM of fludarabine for 24 hours decreased cell viability and increased DNA strand breaks. Treatment of A. melanocarpa and Korean red ginseng extract mixture for 24 hours increased cell viability as compared with single extract treatment. The protective effect of these extracts on cell activity increased in a concentration-dependent manner. DNA strand breaks induced by fludarabine decreased as concentration of extract mixture increased. p-H2AX level, a marker of DNA strand breakage, decreased depending on the concentration of extract mixture. The effect of mixed extract of A. melanocarpa and Korean red ginseng on DNA damage is due to the anti-oxidative effect of A. melanocarpa and signal transmission through glucocorticoid receptor upon binding of saponin of Korean red ginseng.