Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

미세유화약물송달시스템을 이용한 로바스타틴의 생체이용률 향상

윤복영,강복기,정상영,이영원,이시범,황성주,육순홍,강길선,이해방,조선행 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.4

A self-microemulsifying drug delivery system (SMEDDS) was developed to increase the dissolution rate, solubility, and ultimately bioavailability of a poorly water soluble drug, lovastatin. SMEDDS was the mixtures of oils, surfactants, and cosurfactants, which emulsify under conditions of gentle agitation, similar to those which would be encountered in the gastro-intestinal (GI) tract. Various types of self-emulsifying formulations were prepared using four types of oil (Capryol 90, Lauroglycol 90, Labrafil M 1944 CS and Labrafil M 2125), two surfactants (Cremophor EL and Tween 80), and three cosurfactants (Carbitol, PEG 400 and propylene glycol). The efficiency of emulsification was studied using a laser diffraction size analyzer to determine particle size distributions of the resultant emulsions. Optimized formulations selected for bioavailability assessment were Carpryol 90 (40%), Cremophor EL (30%) and Carbitol (30%). SMEDDS containing lovastatin (20 mg and 5 mg) were compared to a conventional lovastatin tablet (Mevacor^? , 20 mg/tab) by the oral administration as prefilled hard gelatin capsules to fasted beagle dogs for in vivo study. The area under the serum concentration-time curve from time zero to the last measured time in serum, AUC_0→24h, was significantly greater in SMEDDS, suggesting that bioavailability increase 130% and 192% by the SMEDDS, respectively. The self-emulsifying formulations of lovastatin afforded the improvement in absolute oral bioavailability relative to previous data of lovastatin tablet formulation. These data indicate the utility of dispersed self-emulsifying formulations for the oral delivery of lovastatin and potentially other poorly absorbed drugs.

연령집단에 따른 노인의 허약(Frailty) 예측요인 분석

조성은 ( Cho Sung Eun ),최은영 ( Choi Eun Young ),오영삼 ( Oh Young Sam ),김영선 ( Kim Young Sun ),김성복 ( Kim Seong Bok ) 한국보건사회연구원 2017 保健社會硏究 Vol.37 No.3

본 연구의 목적은 연령집단에 따른 허약의 예측요인을 분석하는 것이다. 연구의 목적을 위해 2014년 노인실태조사를 사용하여 총 4,123명을 분석의 대상으로 설정하였다. 허약을 예측하는데 있어 연령의 중요성을 고려하여, 본 연구는 대상자를 75세 이상 85세 미만의 중 고령노인집단(n=3,373명)과 85세 이상의 초 고령노인집단(n=750명)으로 분류하여 연구모형을 검증하였다. 중 고령노인집단에서 전 허약노인과 허약노인은 각각 1453명(약 43%), 1,268명(약 37%)으로 나타났으며, 초 고령노인 집단에서 전 허약과 허약노인은 304명(약 40%), 396명(약 52%)으로 나타났다. 회귀분석의 결과 건강단계에서 허약전 단계로 진입하는 예측요인은 중 고령노인집단에서는 여성, 낮은 교육수준, 많은 만성질환, 낙상 유경험자, 높은 우울, 낮은 인지기능이 허약 전 단계를 예측하는 유의한 변인으로 밝혀졌으며 초 고령노인집단은 여성, 높은 우울, 낮은 사회활동이 유의한 예측요인으로 밝혀졌다. 허약 전 단계에서 허약 단계로의 진입을 예측하는 요인으로는 중 고령노인집단은 여성, 높은 연령, 미취업, 많은 만성질환, 낙상 유경험자, 높은 우울, 낮은 인지기능이 유의한 변인이었으며, 초 고령노인 집단은 높은 연령, 많은 만성질환, 높은 우울, 낮은 인지기능이 유의한 예측요인으로 밝혀졌다. 본 연구는 연령의 세분화와 다각적인 요인의 접근으로 허약의 예측요인을 밝히고자 하였다. 연구를 통해 밝혀진 예측요인은 추후 허약에 대한 다각적 연구의 기초자료로서 활용되리라 기대한다. This study aims to investigate the predictors of frailty in different age groups. To address this purpose, this study used the 2014 Survey of the Living Conditions of the Elderly (SLCE). In the research model, 4,123 older adults aged 75 and older were included from the SLCE. All participants were subdivided into two age groups based on their age; the young old group (75-85) and the old-old group (85+). The young old group consisted of 1,453 (43%) pre-frail older adults and 1,268 (37%) frail older adults; the old-old group consisted of 304 (40%) pre-frail older adults and 396 (52%) frail older adults. Our regression analysis showed that in the young-old, being female, lower levels of education, having more chronic diseases, having fall experience, higher depression, lower cognitive functioning significantly predicted transition to pre-frailty from non-frail status. In the old-old, being female, higher depression, lower level of social activity were significant predictors. On the other hand, predictors of transition to frailty from pre-frail status in the young-old were being female, higher age, being unemployed, having more chronic diseases, having fall experience, higher depression, lower cognitive functioning. In the old-old, predictors of transition to frailty from pre-frail status were only higher age, having more chronic disease, higher depression, and lower cognitive functioning. The research findings can be used as a reference point in the making of strategies for preventing frailty.

임신기간중 흰쥐 자궁내막의 Lectin 결합특성에 대한 조직화학적 연구

백상호,김복린,손현준,유영복,이경훈,이화영,이영기,박경한,조사선 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.5

간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

부산지역 중소기업 정보통신기술 활성화방안과 경영조직 개편에 관한 연구

조영복,김진수 釜山 大學校 經營 經濟 硏究所 1994 經營 經濟 硏究 Vol.13 No.1

<요약>본 연구는 중소기업의 정보통신기술 활성화에 영향을 미치는 요인을 분석하기 위하여 이론적 모형을 제시하고, 연구모형을 중심으로 관련요인들 사이의 관계에 대한 가설을 설정한 후 부산지역의 제조기업체를 대상으로 한 실증연구를 통해 가설을 검증한 연구이다. 이 연구의 결과 거시적인 요인에 있어서는 연구가설과는 상이한 결과가 나타나, 부산지역의 중소기업은 정보통신 기술활성화를 위한 조직개편에는 관심을 두고 있지 않거나 정보통신기술 도입이 아직 초기 단계인 것으로 보여진다. 그리고 미시적인 요인인 최고경영층, 중간관리자를 비롯한 사용자 부서 및 전산부서의 전산지식과 전산화에 대한 관심 및 지원수준은 정보통신기술 활성화에 영향을 미치고 있는 것으로 나타났다. 그리고 정보시스템에 대한 관리, 통제가 정보통신기술 활성화와 강한 상관관계가 있는 것으로 나타나 정보화에 관한 관리, 통제면에서 사용자의 자율에 맡기기보다는 보다 강화된 표준화, 관리ㆍ 통제를 통하여 체계적으로 정보화를 추진하는 것이 바람직할 것으로 여겨진다.

조직구조 구성변인에 관한 연구 : 한국의 제조기업체를 대상으로

조영복 인제대학교 1988 仁濟論叢 Vol.4 No.1

In recent years a number of scholoars have attempted to isolate empirically the underling dimensions of organization structure. While many of these researches have used Weber's basic model of bureaucracy as a point of departure, their findings have been somewhat at variance, particularly with respect to the relationships among the various dimensions of bureaucracy. This study focus on the relationships among the three dimensions of organization structure, formalization, complexity, and centraliztion, in Korean manufacturing organizations. In the analysis of 128 manufacturing organizations, the relationships among the dimensions of organization structure are similiar to those of Aston Study(1968), Child's (1972), and Grinyer and Yasai-ardekani(1980). But somewhat difference is found in centralization. This result suggest the cultural differences are not the major variable to differenciate the organization structureal dimensions. The future research is needed to consider more organization structure variables which were not analized in this study and to consider centralization variable more carefully.

김수영, 반여성주의에서 반반의 미학으로

조영복 한국여성문학학회 2001 여성문학연구 Vol.6 No.-

기존의 연구들은 김수영의 여성관이 그의 아내나 거리의 여성, 주변의 여성들에 대한 태도나 김수영의 가족주의적 시각을 통해 드러난다고 보았다. ‘반여성주의’라는 관점의 연구들은 김수영의 신화가 한편으로는 남성적 영웅주의적 시각에서 만들어진 것이며 따라서 그 신화를 한 꺼풀 벗길 필요가 있다는 인식을 보인다. 이 시각은 주로 김수영의 아내에 대한 호칭 문제(‘여편네’)와 여성을 대체로 속물적인 존재로 그리고 있다는 점에 그 근거를 둔다. 그러나 필자의 입장은 좀 다르다. 즉 ‘생활, 현실’의 문제가 중요한 시적 대상이 될 때 비하된 여성이 등장한다는 것은, 한편으로 ‘여성’에 대한 언급이 근대적 삶의 형식에 대한 진술일 수 있음을 의미하는 것이며 다른 한편으로는 그것이 일종의 허영과 허위와 가식, 위악의 텍스트로 표면화되고, 문제의 핵심을 바로 파고들기보다는 그 ‘문제의 에둘러 가기’라는 형식으로 그의 텍스트가 기능할 것을 의미한다. ‘여성’을 대상으로 한 시편들은 한편으로는 연민과 동정으로 다른 하나는 위악과 공격으로 그 어조적 특성을 보여준다. ‘여편네’는 마누라의 속물근성을 지칭하기 위해 사용된 호칭이라기보다는 시인 자신의 속물근성을 회피하고 정신의 긴장 상태를 유지하기 위해 의도적으로 사용된 단어이다. 그것은 악이 아니라 오히려 선의 당위성을 강조하기 위해 존재하는 순간적이고 찰라적인 시간성을 갖는 일시적 개념이다. 어조의 공격성은 결국 시인 자기 자신에게 향하고 있다. 이것은 타인에 대한 공격적인 언어가 사실은 자기 내부에 갇혀서 맴도는 매저키즘적 담론의 한 양상으로 파악될 수 있음을 의미한다. 현실과 생활이 제거된 자리에서 그의 어조는 그리움이나 연정의 형식으로 표출되며 이는 여성적 목소리로 표면화 된다. 이 때의 목소리는 김수영이 권력적이고 계몽적인 근대적 기획의 장면 앞에서 스스로를 반성하고 반성하게 했던 바로 그것, 자기 안의 여성성, 여성적 목소리의 언어이다. ‘여성’은 점점 초월적이고 종교적인 대상으로 변용된다. ‘여성’은 ‘죽음’과 ‘사랑’의 상호 소통과 비약적인 넘나듦을 통해 정의된다. 그의 ‘반여성주의’적 어법은 결국 ‘비약적 사유’를 향해 가는 데 중요한 동기가 되며 이 때의 사변적인 시풍들은 난해시의 전범을 이룬다. Many reserches on Kim Su-Young have emphasized his anti-Feminism. Generally that studies payed attention to his calling his wife, 'yopyunne(여편네)', and his attitude toward his wife and other women. They insist that Kim regarded women as vulgar, egoist and brutal etc. But this thesis is to criticize such points of view. It can be said that Kim's attitude toward women is only a superficial discourse, namely paradox or irony of discorse. Kim named it a aesthetics of half-and-half for himself. He intentionally used this method of naming to maintain a state of tension as poet and to protect himself against snobbery. Kim's reflectional voice is female one and is an inner voice of poet. And that is a very masochist discourse. His female voice is altered into transcendental and religious discourse. At the end, his 'anti-female' discourse is inclined toward an illogical jump in poetic thinking. That is why his discourse is recognized as a model of recondite poetry.

근대 초기 시의 미적 개념 인식과 근대시 장르의 체계화 과정 연구

조영복 우리말글학회 2003 우리말 글 Vol.29 No.-

This thesis is to examine the formation process of the early modern poetry from three different points of view; formal, aesthetic and ideology-historical points. The early modern poetry which has been understood as an imitation of symbolist poems needs to be reviewed from following various angles. First, this thesis proved the fact that the character of coterie of those days was problematic because associates of literacy coterie magazines, such as the Samkwang, the Pyehur, the Jangmichon and Shinsaenghoal etc, are not only literary men but also ideologists. Secondly, the participation of ideologists is related to the formation of early modern poetry's themes, which cannot be easily distinguished from naturalism or mysticism showed in socialist ideology and symbolist poems. Thirdly, this thesis examines concretely the case of Hwang Seok-woo and Namgung Byuk who are estimated as typical symbolist poets. Hwang's poems show an interim step in the way of formal experiment and aesthetic purification of Korean modern poetry. And the issue of self-awakening in Namgung's works can be estimated as a level preparing the formation of modern aestetics, showing the birth of independent human being as modern individual. It is also emphasized in this thesis that the early 1920' poems tend to show excessive romanticism and idealism by stressing so-called 'grandiose self'. This means that it is difficult to regard the romanticism or symbolism of those days as a product of decadent mind which imitated western poetic style. In conclusion, this thesis tries to analyze concretely the forming process of Korean modern poetry from aesthetic, thematic and formal points of view, overcoming the existing attitude of understanding the early modern poems as only one singular discourse of symbolism.

서정적 시와 역동적 서정 : 고은 초기 시의 서정성 연구

조영복 광운대학교 인문사회과학연구소 2002 인문사회과학논문집 Vol.31 No.-

This thesis deals with the relation between lyricism and reality in the Ko-Eun'(고은)s early works. His poems, especially 1980s' poems such as 백두산, 만인보 etc, have been generally regarded as poems of realism or poems of actuallism. Then these poems are said to be locking in lyricism and long on reality. According to this point of view, the lyricism is opposed to realism. But that is not always true. Ko-Eun's 1970s' or 1980s' poems are very actual, but on the other hand they connote lyricism. In his poems realism is closely connected with lyricism. The origin can be found in his early poems. That is to say, dynamic lyricism of his poems turned into realism in 1970∼1980s. This thesis has got at the root of this problem throughout Ko-Eun's early poems.