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Changgeun Kang,Hyungkyoung Lee,Do-Yun Hah,Jung Ho Heo,Chung Hui Kim,Euikyung Kim,Jong Shu Kim 한국독성학회 2013 Toxicological Research Vol.29 No.1
Development of a therapy providing protection from, or reversing gentamicin-sulfate (GS)-induced oxidative stress and nephrotoxicity would be of great clinical significance. The present study was designed to investigate the protective effects of Houttuynia cordata Thunb. (HC) against gentamicin sulfate-induced renal damage in rats. Twenty-eight Sprague-Dawley rats were divided into 4 equal groups as follows: group 1, control; group 2, GS 100 mg/kg/d, intraperitoneal (i.p.) injection; group 3, GS 100 mg/kg/d, i.p. + HC 500 mg/kg/d, oral; and group 4, GS 100 mg/kg/d i.p. + HC 1000 mg/kg/d, oral administration). Treatments were administered once daily for 12 d. After 12 d, biochemical and histopathological analyses were conducted to evaluate oxidative stress and renal nephrotoxicity. Serum levels of creatinine, malondialdehyde (MDA), and blood urea nitrogen (BUN), together with renal levels of MDA, glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were quantified to evaluate antioxidant activity. Animals treated with GS alone showed a significant increase in serum levels of creatinine, BUN, and MDA, with decreased renal levels of GSH, SOD, and CAT. Treatment of rats with HC showed significant improvement in renal function, presumably as a result of decreased biochemical indices and oxidative stress parameters associated with GS-induced nephrotoxicity. Histopathological examination of the rat kidneys confirmed these observations. Therefore, the novel natural antioxidant HC may protect against GSinduced nephrotoxicity and oxidative stress in rats.
Kang, Changgeun,Lee, Hyungkyoung,Yoo, Yong-San,Hah, Do-Yun,Kim, Chung Hui,Kim, Euikyung,Kim, Jong Shu Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.1
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium that are found in cereals and agricultural products. ZEN has been implicated in mycotoxicosis in farm animals and in humans. The toxic effects of ZEN are well known, but the ability of an alkaline Comet assay to assess ZEN-induced oxidative DNA damage in Chang liver cells has not been established. The first aim of this study was to evaluate the Comet assay for the determination of cytotoxicity and extent of DNA damage induced by ZEN toxin, and the second aim was to investigate the ability of N-acetylcysteine amide (NACA) to protect cells from ZEN-induced toxicity. In the Comet assay, DNA damage was assessed by quantifying the tail extent moment (TEM; arbitrary unit) and tail length (TL; arbitrary unit), which are used as indicators of DNA strand breaks in SCGE. The cytotoxic effects of ZEN in Chang liver cells were mediated by inhibition of cell proliferation and induction of oxidative DNA damage. Increasing the concentration of ZEN increased the extent of DNA damage. The extent of DNA migration, and percentage of cells with tails were significantly increased in a concentration-dependent manner following treatment with ZEN toxin (p < 0.05). Treatment with a low concentration of ZEN toxin (25 ${\mu}M$) induced a relatively low level of DNA damage, compared to treatment of cells with a high concentration of ZEN toxin (250 ${\mu}M$). Oxidative DNA damage appeared to be a key determinant of ZEN-induced toxicity in Chang liver cells. Significant reductions in cytolethality and oxidative DNA damage were observed when cells were pretreated with NACA prior to exposure to any concentration of ZEN. Our data suggest that ZEN induces DNA damage in Chang liver cells, and that the antioxidant activity of NACA may contribute to the reduction of ZEN-induced DNA damage and cytotoxicity via elimination of oxidative stress.
Kang, Changgeun,Lee, Hyungkyoung,Hah, Do-Yun,Heo, Jung Ho,Kim, Chung Hui,Kim, Euikyung,Kim, Jong Shu Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.1
Development of a therapy providing protection from, or reversing gentamicin-sulfate (GS)-induced oxidative stress and nephrotoxicity would be of great clinical significance. The present study was designed to investigate the protective effects of Houttuynia cordata Thunb. (HC) against gentamicin sulfate-induced renal damage in rats. Twenty-eight Sprague-Dawley rats were divided into 4 equal groups as follows: group 1, control; group 2, GS 100 mg/kg/d, intraperitoneal (i.p.) injection; group 3, GS 100 mg/kg/d, i.p. + HC 500 mg/kg/d, oral; and group 4, GS 100 mg/kg/d i.p. + HC 1000 mg/kg/d, oral administration). Treatments were administered once daily for 12 d. After 12 d, biochemical and histopathological analyses were conducted to evaluate oxidative stress and renal nephrotoxicity. Serum levels of creatinine, malondialdehyde (MDA), and blood urea nitrogen (BUN), together with renal levels of MDA, glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were quantified to evaluate antioxidant activity. Animals treated with GS alone showed a significant increase in serum levels of creatinine, BUN, and MDA, with decreased renal levels of GSH, SOD, and CAT. Treatment of rats with HC showed significant improvement in renal function, presumably as a result of decreased biochemical indices and oxidative stress parameters associated with GS-induced nephrotoxicity. Histopathological examination of the rat kidneys confirmed these observations. Therefore, the novel natural antioxidant HC may protect against GSinduced nephrotoxicity and oxidative stress in rats.
Changgeun Kang,Hyungkyoung Lee,Yong-San Yoo,Do-Yun Hah,Chung Hui Kim,Euikyung Kim,Jong Shu Kim 한국독성학회 2013 Toxicological Research Vol.29 No.1
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium that are found in cereals and agricultural products. ZEN has been implicated in mycotoxicosis in farm animals and in humans. The toxic effects of ZEN are well known, but the ability of an alkaline Comet assay to assess ZEN-induced oxidative DNA damage in Chang liver cells has not been established. The first aim of this study was to evaluate the Comet assay for the determination of cytotoxicity and extent of DNA damage induced by ZEN toxin, and the second aim was to investigate the ability of N-acetylcysteine amide (NACA) to protect cells from ZEN-induced toxicity. In the Comet assay, DNA damage was assessed by quantifying the tail extent moment (TEM; arbitrary unit) and tail length (TL; arbitrary unit), which are used as indicators of DNA strand breaks in SCGE. The cytotoxic effects of ZEN in Chang liver cells were mediated by inhibition of cell proliferation and induction of oxidative DNA damage. Increasing the concentration of ZEN increased the extent of DNA damage. The extent of DNA migration, and percentage of cells with tails were significantly increased in a concentration-dependent manner following treatment with ZEN toxin (p < 0.05). Treatment with a low concentration of ZEN toxin (25 μM) induced a relatively low level of DNA damage, compared to treatment of cells with a high concentration of ZEN toxin (250 μM). Oxidative DNA damage appeared to be a key determinant of ZEN-induced toxicity in Chang liver cells. Significant reductions in cytolethality and oxidative DNA damage were observed when cells were pretreated with NACA prior to exposure to any concentration of ZEN. Our data suggest that ZEN induces DNA damage in Chang liver cells, and that the antioxidant activity of NACA may contribute to the reduction of ZEN-induced DNA damage and cytotoxicity via elimination of oxidative stress.
변경화,강은정,유창균,Byun, Kyeonghwa,Kang, Eun-Jung,Yoo, Changgeun 한국주거학회 2015 한국주거학회 논문집 Vol.26 No.2
This study aims to analyze space planning of accommodations in seven closed schools involved in experiential activity programs on Jeju Island, a famous tourist destination in Korea. The results are as follows. First, 35 schools had been closed down in Jeju between 1982 and 2015. Among them, four closed schools were sold, three are currently used by the Jeju special self-government provincial office of education, and 25 are being leased by individuals, village organizations, and other entities. However, they have not been well-operated, and so strategies for the effective management or operation of these closed schools should be developed. Second, there are distinctive differences in space planning of accommodations based on the correlation between experiential activities and the types of participants. If the experiential activity participants are mainly individuals or small groups, the closed school is likely to plan double-bed accommodations and individual bathrooms rather than large group rooms. Third, in order to operate experiential activity programs, closed schools require four different spaces for experiential activities, lodging, dining, and sanitation. The largest space available in each school building is used for experiential activities, and original classrooms are still in use. According to the standard drawing of school architecture, standard classroom size since 1997 has been $7.5{\times}9$ meters, and lodging rooms have mainly been re-planned based on the module of the standard-sized classroom. School cafeterias are utilized as dinning and kitchen spaces. For sanitation purposes, original school restrooms are still used by experiential program participants, while the shower rooms are newly equipped.
정인오(Ino Jung),하승철(Seungchul Ha),강재구(Jaegu Kang),박창근(Changgeun Park) 항공우주시스템공학회 2018 항공우주시스템공학회 학술대회 발표집 Vol.2018 No.4
본 논문에서는 해상 환경에서 소형 수직이착륙 항공기가 함상에 랜딩 후 미끄러짐과 뒤집힘을 방지할 수 있는 전기식 착함장치 개발 및 시험 전과정에 대한 개요를 논하고 있다. 해상 환경은 육상과 달리 심한 바람과 파도에 따른 함상의 운동(motion)이 존재하기 때문에 항공기의 미끄러짐과 뒤집힘(toppling)의 위험이 존재한다. 현재 국내에서는 해외에서 개발된 항공기 및 하푼을 적용하여 해상 환경하의 함상에 사용 중이다. 이에 국내에서 개발되는 소형 수직이착륙 항공기에 적용 가능하고, 항공기의 입력 전원을 이용하여 사용할 수 있는 전기식 하푼을 개발하였다.