Low-Powered pH-Stable Nano-electrokinetically Enhanced Lateral Flow Assay for COVID-19 Antigen Test

Kim Kang Hyeon,유용경,Lee Na Eun,Lee Junwoo,Kim Cheonjung,Lee Seungmin,Kim Jinhwan,Park Seong Jun,Lee Dongtak,이상원,Kim Hyungseok,허돈,Yoon Dae Sung,Lee Jeong Hoon 한국바이오칩학회 2023 BioChip Journal Vol.17 No.3

Lateral fl ow assay (LFA) is a popular diagnostic system used in point-of-care testing (POCT) due to its low cost and portability. However, LFA has limited sensitivity and detection limits, making it challenging to detect low virus titers. Preconcentration through nano-electrokinetic (NEK) techniques have been proposed as a promising solution to improve the sensitivity of LFA. Nevertheless, the acidic conditions used in NEK operations may reduce the specifi city and sensitivity of LFA immunoassays. To address these limitations, an integrated LFA kit, the NEK-enhanced LFA (PcNEK–LFA), has been introduced. This kit features a pH-controlled structure designed to facilitate sample preconcentration. Biomarkers and AuNPs are electrokinetically preconcentrated in the PcNEK–LFA platform to increase the concentration of the test line and Ag–Ab binding events, resulting in enhanced performance. The pH-controlled PcNEK–LFA platform was evaluated using salivary human chorionic gonadotropin beta (β-hCG) and COVID-19 Ag samples, achieving a preconcentrating factor of approximately 10 and a sensitivity enhancement of 55.42%, and a preconcentrating factor greater than 10, respectively. The pH-controlled PcNEK–LFA platform provides an eff ective solution to overcome the limitations of LFA for POCT. In addition, it improves its sensitivity and detection limit, signifi cantly enhancing the accuracy and reliability of POCT, particularly for COVID-19 screening tests. As a result, this platform may play a pivotal role in addressing current and future healthcare challenges, facilitating rapid diagnosis and treatment of infectious diseases.

Diagnosis of Lymphoid Malignancy by PCR for Analysis of Antigen Receptor Rearrangement after Blood Transfusion in a Dog with Acute Lymphocytic Leukemia

Kim, Suhee,Kim, Hyunwoo,Lee, Soo-Hyeon,Cho, Ilhan,Kang, Seongwoo,Bae, Junwoo,Kim, Woosun,Ahn, Soomin,Choi, Jihye,Kim, Sang-Ki,Do, Yoonjung,Yoo, Jae Gyu,Park, Jinho,Yu, DoHyeon 한국조명·전기설비학회 2017 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>Acute lymphocytic leukemia (ALL) is uncommon lymphoid malignancy in dogs, and its diagnosis is challenging. A 14-year-old spayed female mixed breed dog was transferred to a veterinary medical teaching hospital for an immediate blood transfusion. The dog showed lethargy, pale mucous membranes, and a weak femoral pulse. Complete blood count revealed non-regenerative anemia and severe leukopenia with thrombocytopenia. ALL was tentatively diagnosed based on the predominance of immature lymphoblasts on blood film examination. For confirmation of lymphoid malignancy, PCR for antigen receptor rearrangement (PARR) on a peripheral blood sample and flow cytometry analysis were performed after blood transfusion. Flow cytometry analysis revealed that lymphocyte subsets were of normal composition, but PARR detected a T-cell malignancy. The dog was diagnosed with ALL and survived 1 wk after diagnosis. In conclusion, after blood transfusion, flow cytometry was not a reliable diagnostic method for an ALL dog, whereas PARR could detect lymphoid malignancy. Our results suggest that PARR should be the first-line diagnostic tool to detect canine lymphoid malignancy after a blood transfusion.</P>

Rapid exacerbation of renal function after administration of hydroxyethyl starch in a dog

BAE, Junwoo,SOLIMAN, Mahmoud,KIM, Hyunwoo,KANG, Seongwoo,KIM, Woosun,AHN, Soomin,CHO, Kyoungoh,CHOI, Jihye,KIM, Suhee,PARK, Jinho,KIM, Sangki,DO, Yoonjung,YOO, Jaegyu,YU, Dohyeon JAPANESE SOCIETY OF VETERINARY SCIENCE 2017 The Journal of veterinary medical science Vol.79 No.9

<P>Hydroxyethyl starches (HES) are commonly used synthetic colloidal solution in veterinary medicine. Despite of possible adverse effect to kidney injury in human, there is no report about nephrotoxic effects of HES in dogs. HES was administered to a Golden retriever (4-year-old, intact male) with ascites in order to increase plasma osmolality. Initially, the dog was mild azotemic, however, kidney function was rapidly deteriorated after several days of HES administration. Finally, histopathological examination revealed remarkable osmotic nephrosis. In the case reported herein, acute kidney injury was remarkably developed after HES administration. Clinical and histopathologic findings of acute kidney injury support nephrotoxic effects of HES to a dog.</P>

낙상 감지 프로시저가 포함된 공유 전동킥보드 어플리케이션 서비스 구조 제안

김준우(Junwoo Kim),조주희(Joohee Cho),김승률(Seungryul Kim),이정우(Jungwoo Lee),이재현(Jaehyun Lee),최화영(Hwayoung Choi),최재영(Jaeyeong Choi),김남기(Namki Kim) 한국정보기술학회 2023 Proceedings of KIIT Conference Vol.2023 No.6

다층 구조 엑스선 센서 기반 기저 물질 분해 영상의 산업적 응용 가능성에 관한 연구

김준우(Junwoo Kim),유승준(Seungjun Yoo),김진우(Jinwoo Kim),김호경(Ho Kyung Kim) 대한기계학회 2021 大韓機械學會論文集A Vol.45 No.7

서로 다른 두 에너지로 2회 촬영한 엑스선 영상을 조합하여 특정 물질을 억제 또는 강조하는 이중 에너지 기법을 산업용 비파괴 검사에 적용해 보았다. 본 논문에서는 검사 시간, 엑스선관 부하 등 검사 효율의 개선을 위해 2회 촬영 대신, 두 개의 센서를 포갠 후 1회 촬영으로 이중 에너지 영상을 얻는 방법을 제안하였다. 두 기저 물질의 두께 조합으로 영상을 표현하는 기저 물질 분해 방법을 영상 재구성 알고리즘으로 이용하였으며, 두 기저 물질로 PMMA와 Al을 선정하였다. 직접 제작한 Al, Cu, Au, Pb 등 다양한 금속 물질로 구성된 팬텀을 이용하여 제안한 기법의 성능을 살펴본 결과, 1회 촬영 기법이 기존 2회 촬영 기법에 버금가는 성능을 보여줄 수 있음을 확인하였다. PCB 등 다양한 전자 부품 샘플에 대한 테스트에서도 물질 구분 영상 재구성의 가능성을 확인하였다. 향후 다층 구조 엑스선 검출기의 대면적 제작이 이루어진다면 이중 에너지 물질 영상 재구성을 통해 관심 물질을 특정하여 결함을 보다 쉽고 빠르게 검출할 수 있을 것이다. The potential of dual-energy (DE) x-ray imaging, which is used in various applications from medical imaging to industrial defect inspection, is investigated using laboratory-scale experiments. To reduce the inspection time and large heat-load of the x-ray tube, we propose to use a multi-layered detector to obtain two different energy images at a single exposure, in conjunction with the basis-material decomposition algorithm for a quantitative DE reconstruction. The DE images obtained for the home-made phantom comprising Al, Cu, Au, and Pb sheets using the proposed single-exposure method show that the overall performance of material identification is comparable to that of the conventional dual-exposure method. The demonstration DE images of PCB samples show the feasibility of DE imaging with a multi-layered detector. The proposed method may be preferable when the imaging speed and x-ray tube load are primary concerns in quantitative x-ray inspection systems.

대형 강관 엑스선 검사를 위한 대조도 향상 알고리즘의 영상 특성에 관한 연구

김준우(Junwoo Kim),김균엽(Gyun Youp Kim),김창수(Changsoo Kim),김호경(Ho Kyung Kim) 한국비파괴검사학회 2021 한국비파괴검사학회 춘계학술대회 초록집 Vol.2021 No.6

다양한 산업분야에 사용되는 대형 강관은 탄소 또는 저 합금 강판을 원형으로 성형하고, 이음매를 submerged arc welding (SAW) 방식으로 용접하여 제조된다. 대형 강관의 품질을 위해 용접부의 엑스선 투과 검사가 일반적으로 수행되며 엑스선 영상은 인정된 비파괴 검사 판독자의 판독 하에 결함 유무가 마킹된다. 하지만 강관용접 시 날씨, 온도, 습도 등과 같은 환경적인 요인과 용접조건 등에 의해 용접부의 밀도가 증가하게 되면 콘트라스트가 저하되어 정상 혹은 결함 식별이 어려워진다. 뿐만 아니라 엑스선 투과 검사 설비의 운영과 관련된 부분은 자동화가 가능하나 판독자에 의해 엑스선 영상이 식별되기 때문에 판독자의 피로도에 따라 생산성 저하 및 인적 오류는 더욱 커지게 되며 결국 사업 손실로 이어진다. 현재, 컴퓨터 기계학습 등으로 자동 판독에 관한 여러 연구가 진행되고 있지만 필수적으로 판독자의 재판독이 이루어져야 하는 것은 사실이다. 따라서, 판독자가 바라보는 엑스선영상의 콘트라스트를 향상시키기 위해 multi-scale contrast enhancing (MCE) 방법을 제시하고자 한다. 이 방법은 엑스선 영상을 다양한 스케일로 분해한 후 각 스케일 영상에서의 특징을 고려해서 콘트라스트를 향상시키는 방법으로 모든 스케일에서의 콘트라스트를 향상시킬 수 있으며 원본 영상에서 식별할 수 없는 다양한 특징들을 분석할 수 있어 결함 식별에 도움이 될 수 있을 것이다. 따라서 본 연구에서는 MSCI 방법을 대형 강관 엑스선 영상에 적용하고자 하며 적용 전후의 영상을 분석하고, 대형 강관 엑스선 검사에 적합한 최적 파라미터에 대해 연구하고자 한다. Steel pipes used in various industries are manufactured by forming carbon or low-alloy steel plates in a circular and welding the joints in submerged arc welding (SAW). For the quality of steel pipes, x-ray penetration testing of welds is generally performed, and x-ray images are read by recognized non-destructive test (NDT) readers and marked for defects. However, if the density of the welding part increases due to environmental factors such as weather, temperature, humidity, etc. during steel-pipe welding, the contrast decreases, making it difficult to identify normal or defects. Furthermore, the part related to the operation of the x-ray penetration inspection facility can be automated, but because x-ray images are identified by the reader, productivity degradation and human error become greater with reader fatigue, which eventually leads to business loss. Currently, several studies are being conducted on automatic reading, such as machine learning, but it is true that re-reads of the NDT reader must be carried out. Therefore, we present a multi-scale contrast enhancing (MCE) method to improve the contrast of x-ray images viewed by NDT readers. This method can improve the contrast at all scales by decomposing the x-ray images into various scales and then considering the features in each scale image to improve the contrast, and can analyze various features that cannot be identified in the original image, which may help to identify defects. In this study, we apply the MSCI method to steel-pipe x-ray images, analyze x-ray image with or without MSCI, and investigate optimal parameters suitable for steel-pipe x-ray inspection.

대형 강관 엑스선 검사 시스템의 신호 및 노이즈 분석

김준우(Junwoo Kim),김균엽(Gyun Youp Kim),김호경(Ho Kyung Kim),김창수(Changsoo Kim) 한국비파괴검사학회 2021 한국비파괴검사학회지 Vol.41 No.6

배수로 및 원자력에 사용되는 대형 강관은 submerged arc welding (SAW) 용접 강관으로 용접부의 건전성이 강관의 품질수준을 결정하며 엑스선 투과 검사가 필수적인 사항으로 요구된다. 최근 명례 산업단지에 설치된 대형 강관 고에너지 엑스선 검사 시스템은 생산성의 효율을 위해 엑스선 튜브와 디텍터를 대형 강관 끝단부와 몸체부에 각각 하나씩 설치되어 있는 듀얼 시스템을 사용하고 있으며 생산성을 약 1.5배 가량 개선하였다. 고에너지 엑스선을 사용한 비파괴 검사의 경우 콘트라스트 및 공간 해상도 등에서 높은 영상 성능을 요구하지만 고에너지 영역에서는 콤프턴 산란 반응이 지배적이다. 따라서, 새로 설치된 엑스선 검사장치에 대해 국제표준인 미국재료시험학회(American Society for Testing and Materials, ASTM) 및 미국기계학회(American Society of Mechanical Engineers, ASME)에서 제시하고 있는 팬텀을 기반으로 다양한 파라미터에 대한 실험을 수행하여 초기 영상을 획득하고, 그 영상의 신호 및 노이즈를 분석하였다. 더불어 디지털 래디오그라피에서 사용되고 있는 변조전달함수(modulation-transfer function, MTF), 노이즈파워스펙트럼(noise-power spectrum, NPS)를 측정하였다. MTF는 철 에지-나이프 팬텀 영상으로부터 계산되었고, NPS는 강관 영상의 uniform한 영역에서 계산되었다. MTF 성능은 차단 주파수에서 약 5%였으며 kV 증가에 의존하였고, NPS는 kV와 mA 모두에 영향을 받았다. 보다 정량적인 해석을 위해 다양한 대형 강관 두께에 대한 실험 및 분석이 필요할 것이다. Steel pipes used for drainage and nuclear power are welded using submerged arc welding (SAW) method. The soundness of the welds determines the quality of the steel pipes, and X-ray inspection is essential for healing the welding quality. Recently, a high-energy X-ray penetration inspection system for steel pipes was installed in the Myeong-rye industrial complex that utilizes dual X-rays with one X-ray tube at the end of the steel pipe and the other at the body for production efficiency, resulting in improved productivity by 1.5 times. Non-destructive testing with high-energy X-rays requires high image performance, for instance, contrast and spatial resolution. However, the Compton scattering interaction is dominant in the high-energy field. Accordingly, the newly installed X-ray inspection system has been tested on various parameters based on the phantom suggested in the international standards presented by the American Society for Testing and Materials (ASTM) and the American Society of Mechanical Engineers (ASME) to obtain the initial images and to analyze the signal and noise in the images. In addition, methods of measuring the modulation-transfer function (MTF) and the noise-power spectrum (NPS) used in digital radiography were investigated for a more accurate quantitative analysis. The MTF was calculated from steel edge-knife phantom images, and the NPS was calculated from the uniform area of the steel-pipe image. The MTF performance was approximately 5% of the cutoff frequency and relied on an increase in the kV. The NPS was affected by both the kV and the mA. For a more reliable quantitative analysis, additional experiments and evaluations on various steel-pipe thicknesses are required.

LVDC 지중전력케이블 실증 시험

김태민(Taemin Kim),이준우(Junwoo Lee),조영표(Youngpyo Cho),김석웅(Seokwoong Kim),김홍주(Hongjoo Kim),조진태(Jintae Cho),김주용(Juyong Kim) 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.10

온보드 SAR 상황인식 및 상황판단 기술개발 방향

김덕진(Duk-jin Kim),김휘송(Hwisong Kim),김준우(Junwoo Kim),송주영(Juyoung Song),이혁빈(Hyukbin Lee),안상호(Sangho Ahn),우무현(Mohyun Woo),공영균(Younggyun Kong),김진우(Jinwoo Kim) 한국항공우주학회 2023 한국항공우주학회 학술발표회 논문집 Vol.2023 No.6

Effects of the technique parameters on the imaging performance of the dual-energy chest radiography

Kim Changsoo,Yang Sung-Hee,Kim Junwoo 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.78 No.9

Conventional chest-radiography images are the most basic X-ray imaging system for diagnosing lung diseases. However, space-averaging increases due to the overlapping of anatomy structures as three-dimensional information are reduced in two-dimensions. A dual-energy X-ray imaging (DEI) technique for resolve space-averaging is used to increase the conspicuity of the lesion (i.e., lung nodule and calcification) by suppressing anatomical background noise. DEI techniques perform the weighted log-subtraction using the attenuation characteristics of the material for low- and high-kilovoltage-peak (kVp) X-rays. And the weighting factor is calculated to suppressing anatomical background. The objective of this research is to increase the lesion conspicuity in lung regions by optimizing DE technique parameters. DE technique has been applied to three phantoms mimicking the average, thick, and thicker adult chest thicknesses while maintaining the total incident dose equivalent to that used to obtain a single chest radiograph. Investigating DE technique parameters include the added filtration, the low- and high-kVp pair, and the dose allocation between low- and high-kVp setups at the total dose equal to or less than that of the conventional chest radiograph. And the DE image performances investigated through contrast-to-noise ratio, modulation-transfer function, noise-power spectrum, and detective quantum efficiency.