Pharmacognostical Evaluation of Clerodendrum viscosum (Vent.)

Richard Lobo,C. Dinesh Kumar,I. S. R. Punitha,K. Rajendran,Arun Shirwaikar,Annie Shirwaikar 한국생약학회 2006 Natural Product Sciences Vol.12 No.2

study presents a detailed pharmacognostical study of the root and leaf of the crude drugClerodendrum viscosum Vent. (Verbenaceae), an important plant in the Indian system of medicine. The root andleaf samples were studied using light and confocal microscopy, WHO recommended-physicochemicaldeterminations, and authentic phytochemical procedures. The physicochemical, morphological, and histologicalparameters presented in this paper may be proposed as parameters to establish the authenticity of C. viscosum rootand leaf, and may possibly help to diferentiate the drug from its other species.KeywordsClerodendrum viscosum, Verbenaceae, pharmacognosy, root and leaf

An Identity Based Pharmacognostical Profile of folium Annona squamosa

C. Dinesh Kumar,K. Rajendran,Richard Lobo,Annie Shirwaikar 한국생약학회 2005 Natural Product Sciences Vol.11 No.4

The leaves of Annona squamosa Linn. (Annonaceae), comonly called as custard apple, areinsecticidal activity, especially for destroying lice. This paper presents a detailed pharmacognostical study of thecrude drug folium Annona squamosa. The samples were studied using procedures of light, confocal microscopy,WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physico-chemical, morphological and histological parameters presented in this paper may be proposed as parameters toestablish the authenticity of Annona squamosa leaf and may possibly help to differentiate the drug from itsadulterants.

Wing and leg amputation induced oviposition pattern of tropical tasar silk moth, Antheraea mylitta Drury (Lepidoptera: Saturniidae) in oviposition devices – Earthen cups and paper sheets

Mohanraj Soundappan S,Dinesh Kumar,Bhagavanulu M.V.K.,Chandrashekharaiah M.,M. S. Rathore,Srinivas C. 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Antheraea mylitta Drury (Lepidoptera: Saturniidae) is a tropical semi-domesticated wild tasar silkworm reared by marginal tribal farmers of India. Due to improper egg laying frequency, oviposition is generally kept for three continuous days after amputating the gravid moth wings (W – ) in earthen cups (C). In order to systematize the oviposition frequency, wing cut with leg cut (W – L – ) was performed in ecorace Daba trivoltine (DTV) completing three life-cycles in a year. Oviposition of (C)W – L – was observed in all three grainages during the year 2020 and compared with (C)W – moths. Oviposition was also accomplished on plain paper sheets (S). It is observed that (C) W – obtained 63–67% and (C)W – L – obtained 72–80% egg laying on first day. Similarly, (S)W – and (S)W – L – ob tained 68–71% and 74–83% on first day. Further, significant high oviposition was observed within first four hours in both earthen cups and on paper sheets by W – L – moths laying 49–69% and 55–65% eggs compared to W – laying 25–45% and 39–44% eggs, respectively. A total of 44–51, 47–54, 44–48 and 46–52 eggs/g moth weight was obtained in (C)W – , (C)W – L – , (S)W – and (S)W – L – , respectively in three consecutive grainage. Oviposition of W – moths in earthen cups and on paper sheets are not significantly different indicating earthen cups in contemporary tasar grainage could be replaced with paper sheets. Thus, the paper demonstrates for the first time a fast, efficient and scalable cellular oviposition of A. mylitta on paper sheets comparable to Bombyx mori

Mapping of Brain Activations to Rectal Balloon Distension Stimuli in Male Patients with Irritable Bowel Syndrome Using Functional Magnetic Resonance Imaging

( Anupam Guleria ),( Arun Karyampudi ),( Rajan Singh ),( Chunni L Khetrapal ),( Abhai Verma ),( Uday C Ghoshal ),( Dinesh Kumar ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.3

Background/Aims Irritable bowel syndrome (IBS) is associated with exaggerated cerebral response including emotional processing following visceral stimulation; though data on this issue is available in female IBS patients, it is scanty among males. Hence, we aimed to study brain response of male IBS patients following rectal balloon distension as compared to healthy controls using functional magnetic resonance imaging (fMRI). Data between diarrhea and constipation predominant IBS (IBS-D and IBS-C) were also compared. Methods Rectal balloon distension threshold was assessed in 20 male IBS patients (10 IBS-C and 10 IBS-D) and 10 age-matched male healthy controls. Subsequently, fMRI on all the participants was performed at their respective rectal pain threshold. The fMRI data were analysed using the Statistical Parametric Mapping software. Results IBS patients showed greater cerebral activations in insula, middle temporal gyrus, and cerebellum in the left hemisphere compared to healthy controls. Neural activation was found in bilateral precuneus/superior parietal lobules in controls but not in patients with IBS. The brain activation differed among IBS-C and IBS-D patients; while the right mid-cingulate cortex was activated in IBS-C, the left inferior orbito-frontal cortex, left calcarine, and bilateral fusiform gyri were activated among patients with IBS-D following rectal balloon distension. Conclusions Brain response to rectal balloon distension differed among male patients with IBS and controls and among patients with IBS-C and IBS-D. Differential activation among patients with IBS-C and IBS-D was seen in the brain regions controlling affective motivation, homeostatic emotions, and autonomic responses to pain. (J Neurogastroenterol Motil 2017;23:415-427)

Anti allergic and anti-inflammatory effects of the fruits of Randia dumetorum Lamk

Dinesh kumar,Satish C. Mudgade,Zulfiqar Ali Bhat,Santosh S. Bhujbal,R. Rub,이철훈,손은화 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.3

Randia dumetorum Lamk. commonly known as Madana in Sanskrit and widely distributed throughout India. In Ayurveda, Randia dumetorum Lamk. is used in treatment asthma, rhinitis, bronchatitis, cold, cough, pain,inflammation etc. The aim of this study: was to assess the anti allergic effects of the fruit of Randia dumetorum Lamk. Allergy and anaphylaxis are the most responsible factor for diseases like asthma, rhinitis, bronchatitis, cold,cough, pain, inflammation etc. Asthma is an inflammatory disease of lungs and the hallmarks of the disease are the increased inflammatory cell infiltration into the airways and poor respiratory function although there is a possibility that Randia dumetorum Lamk. may have an inhibitory effect on lung inflammation and allergic mediators. The effects of Randia dumetorum Lamk. on allergy and asthma have not been fully investigated. In the present study, we investigated the antiallergic and antiinflammatory activity of Randia dumetorum Lamk. extract and its fraction on milk induced leucocytosis and eosinophilia in mice, passive paw anaphylaxis and mast cell degranulation in rat models. Randia dumetorum Lamk. extract was obtained from dried and powdered fruits of Randia dumetorum Lamk. using 95% methanol and its fractions were obtained by using increasing polarity of solvents like Petroleum ether, chloroform,ethylacetate and methanol. Milk was used to induce leucocytosis and eosinophilia in mice model, eggalbumin was used as sensitizer in passive paw anaphylaxis in rat model and clonidine was used to degranulate the mast cells in rat model. We examined the effects of Randia dumetorum Lamk. extract and its fraction on milk induced leucocytosis and eosinophilia in mice, passive paw anaphylaxis and mast cell degranulation in rats. The extract and its fractions significantly inhibited leukocytosis and eosinophilia in blood of mice. The extract and its fractions also significantly inhibited the passive paw oedema and mast cell degranulation in rats. Methanol extract showed dose dependent significant activity (**p<0.01). Amongst the fractions, chloroform fraction showed the most significant activity followed by ethyl acetate and residual methanol fraction with intermediate activity and finally the petroleum ether fraction with the least or negligible activity (*p<0.05, **p<0.01). These results suggest the possibility of extract and its fraction can exert suppressive effects on allergy, inflammation and asthma and may provide evidence that Randia dumetorum Lamk. is a useful agent for the treatment of allergic airway disease. Hence it would be a good path for the isolation of most bioactive principle for the management of allergic airway disease.

Genetic Variability and Bottleneck Analysis of Jakhrana Goats by Microsatellite DNA Fingerprinting

Dinesh Kumar,A. K. Pandey,S. P. S. Ahlawat,Geetu Malik,Rekha Sharma,Digpal Singh Gour,S. P. Dixit,N. K. Verma,R. A. K. Aggarwal,Neelam Gupta,B. N. Shahi,S. C. Gupta 한국유전학회 2005 Genes & Genomics Vol.27 No.3

Anti allergic and anti-inflammatory effects of the fruits of $Randia$ $dumetorum$ Lamk

Kumar, Dinesh,Mudgade, Satish C.,Bhat, Zulfiqar Ali,Bhujbal, Santosh S.,Rub, R. 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.3

Reduced graphene oxide modified smart conducting paper for cancer biosensor

Kumar, Saurabh,Kumar, Suveen,Srivastava, Saurabh,Yadav, Birendra K.,Lee, Seung H.,Sharma, Jai G.,Doval, Dinesh C.,Malhotra, Bansi D. Elsevier 2015 Biosensors & bioelectronics Vol.73 No.-

<P><B>Abstract</B></P> <P>We report results of the studies relating to the fabrication of a paper based sensor comprising of poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and reduced graphene oxide (RGO) composite. The effect of various solvents like methanol, ethylene glycol and H<SUB>2</SUB>SO<SUB>4</SUB> on the electrical conductivity of PEDOT:PSS coated Whatman paper has been investigated. The conductivity of this solution processed conducting paper significantly increases from ~1.16×10<SUP>−4</SUP> Scm<SUP>−1</SUP> up to ~3.57×10<SUP>−2</SUP> Scm<SUP>−1</SUP> (~300 times) on treatment with ethylene glycol. The observed significant increase in electrical conductivity is due to conformational rearrangement in the polymer and is due to strong non-covalent cooperative interaction between PEDOT and the cellulose molecules. Further, incorporation of RGO into the conducting paper results in improved electrochemical performance and signal stability. This paper electrode is a promising alternative over the expensive conventional electrodes (ITO, gold and glassy carbon), that are known to have limited application in smart point-of-care (POC) devices. This low cost, flexible and environment friendly conducting paper based biosensor utilized for cancer biomarker (carcinoembryonic antigen, CEA) detection reveals high sensitivity of 25.8µAng<SUP>−1</SUP> mLcm<SUP>−2</SUP> in the physiological range, 1–10ngmL<SUP>−1</SUP>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Solution processed reduced graphene oxide modified conducting paper sensor is reported for cancer detection. </LI> <LI> This paper sensor is exceptionally low cost, flexible and disposable. </LI> <LI> It exhibits high sensitivity of 25.8µAng<SUP>−1</SUP> mLcm<SUP>−2</SUP> in the detection range of 1–10ngmL<SUP>−1</SUP> for cancer biomarker (carcinoembryonic antigen, CEA) detection. </LI> <LI> This conducting paper electrode is a promising alternative over the expensive conventional electrodes (ITO, gold and glassy carbon) for application to POC devices. </LI> </UL> </P>

Drastic photocatalytic degradation of methylene blue dye by neodymium doped zirconium oxide as photocatalyst under visible light irradiation

Dhandapani, C.,Narayanasamy, R.,Karthick, S.N.,Hemalatha, K.V.,Selvam, S.,Hemalatha, P.,kumar, M. Suresh,Kirupha, S. Dinesh,Kim, Hee-Je Elsevier 2016 OPTIK -STUTTGART- Vol.127 No.22

<P><B>Abstract</B></P> <P>Nanocrystalline ZrO<SUB>2</SUB> and neodymium doped ZrO<SUB>2</SUB> (Nd–ZrO<SUB>2</SUB>) were successfully prepared by a polymer- assisted, sol-gel method and characterized by XRD, SEM, HRTEM, XPS and UV–vis techniques. X-Ray diffraction studies revealed the formation of highly crystalline structures of ZrO<SUB>2</SUB> and (Nd–ZrO<SUB>2</SUB>) samples. SEM images proved the evenly distributed nano - structured, spherically - shaped ZrO<SUB>2</SUB> particles of size 40nm, which was reduced to 20nm, when doped with neodymium. Light absorption properties of the nanoparticles were studied by UV–vis spectrophotometer. Nd - doped ZrO<SUB>2</SUB> was having a band gap of 2.8ev, while that of ZrO<SUB>2</SUB>, was 2.6ev. Thus, Nd substituted ZrO<SUB>2</SUB> effected substantial shifting of the absorption edge of zirconium oxide to red and resulting in the enhancement of visible light absorption The increase in band gap of Nd–ZrO<SUB>2</SUB> was resulted in an increasing light absorbtion tendency of Nd−ZrO<SUB>2</SUB>, which promoted the photocatalytic efficiency of it. XPS results confirmed substitution of Nd in ZrO<SUB>2</SUB>. Photocatalytic results for the degradation of methylene blue had indicated the fact that neodymium substitution dramatically enhanced the catalytic activity of zirconium oxide. This means that the neodymium − substituted zirconium oxide has the highest photocatalytic activity under visible light irradiation.</P>

Histological and Physico-Chemical Evaluation ofHybanthus enneaspermus (L.) F. Muell

V. B. Narayanaswamy,C. Dinesh Kumar,M. Manjunath Setty,Annie Shirwaikar 한국생약학회 2006 Natural Product Sciences Vol.12 No.2

paper presents a detailed pharmacognostical study of the plant drug Hybanthus enneaspermusstudied using procedures of light, confocal microscopy, WHO recomended physico-chemical determinationsand authentic phytochemical procedures. The physico-chemical, morphological, and histological parameterspresented in this paper may be proposed as parameters to establish the authenticity of H. enneaspermus and maypossibly help to diferentiate the drug from its adulterants.KeywordsHybanthus enneaspermus, Violaceae, pharmacognosy, stem and leaf