Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

기능성 소화불량증환자에서 Winstal® 투여에 관한 임상경험

이창형,김영탁,금민수,권중구,안병철,윤영미,권영오,김성국,최용환,정준모 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.3

목적 : 기능성 소화불량증은 소화, 흡수등 장관의 기능적인 이상과 관련되어 있을 것으로 생각되며 복합소화효소제(Winstal®)를 투여하여 그 임상효과를 평가하고자 본 연구를 실시하였다. 대상 및 방법 : 1994년 3월 부터 6월까지 경북대학교병원 내과를 방문한 기능성 소화불량증을 호소하는 환자 20명을 대상으로 Winstal®을 1회 1정씩, 1일 3회 식후 30분내에 경구로 2주간 투여하였다. 결과 : 소화불량증의 증상은 복부불쾌감, 복부팽만감, 식욕부진 및 오심, 복부동통, 공기연하증, 고창 및 구토순이었으며, 증상의 개선은 복부불쾌감이 76.4%(13/17)로 가장 높았으며, 그 다음으로 복부팽만감 및 공기연하증이 66.5%(10/15, 6/9)이었고, 오심 61.5%(8/13), 식욕부진 53.8%(7/13), 복부동통 41.6%(5/12), 고창 37.5%(3/8)이었다. 각 환자별 종합적인 증상의 개선도는 현저한 개선이 2예(10%), 중등도 개선이 3예(15%), 약간개선이 11예(55%), 불변이 4예(20%)이었으며 종합적인 유효율은 80%(16/20)이었다. 부작용은 한 예에서도 관찰되지 않았다. 결론 : 본 제제는 기능성 소화불량증 환자의 증상개선에 추천할 만한 유효한 약제로 생각된다. Dyspepsia is a common symptom in gastroenterologic practice and trigger for numerous consultations with physician. The treatment of chronic functional dyspesia is unsatisfactory. This study was performed to evaluate the efficacy of the digestive compound (Winstal®) on 20 functional dyspepsia patients. On open trial, all patients were given 6 tablets daily for 2 weeks and we evaluated the efficacy of this preparation according to the degree of the improvement. The improvement of symptoms was 76.4%(13/17) in abdominal discomfort, 66.5%(10/15, 6/9) in abdominal distension and aerophagia, 61.5%(8/13) in nausea, 53.8%(7/13) in abdominal pain, and 37.5% (5/12) in flatulence, respectively. Overall effectiveness of subjective symptoms was 80% (16/20) and there were no untoward effects of the preparations during this study. As a result, We think that this preparation is an effective one to relieve symptoms of functional dyspepsia.

웹 기반 BASIC 프로그래밍 실습 환경

이재선,창병모 숙명여자대학교 자연과학연구소 2000 자연과학논문집 Vol.- No.11

프로그래밍 학습을 원하는 학 습자들은 컴파일러나 인터프리터와 같은 언어처리기를 먼저 갖추어야 한다. 이는 학습자들에게 언어처리기 설치에 대한 부담과 업그레이드 문제 등 학습 이외의 부담을 주게된다. 본 논문에서는 별도의 언어처리기를 설치하지 않아도 웹 상에서 프로그래밍 학습과 실습이 모두 가능한 실습 환경을 구현하였다. 구현된 시스템에서는 BASIC 언어에 대한 학습과 실습이 웹 상에서 모두 가능하도록 Java애플릿으로 구현된 BASIC인터프리터를 제공해준다. 또한 애플릿으로 구현된 편집창과 BASIC 인터프리터간의 통신을 통해 서버로부터 예제 파일을 불러와 편집이 가능하며, 편집한 프로그램을 인터프리터로 전송시켜 자동 실행할 수 있다. 이러한 기능들을 통하여 학습자들에게 웹 상에서 효과적으로 프로그래밍 언어를 학습 할 수 있는 환경을 제공해 준다. Leamers who want to learn programming should install language processors like compilers or interpreters first. This gives an extra burden other than programming learning. In this paper, we design a virtual programming environment that enables learners to learn and practice programming on the web without installing languages processors. This system provides a BASIC interpreter as Java applet so as to learn and practice programming on the web. It also provides an editor so as to edit example files brought from the server. Edited programs can also be sent to interpreter and executed automatically. These functions make it possible to learn programming language more effectively on the virtual programming environment.

반지능적인 백트랙킹을 지원하는 WAM의 설계

이수현,창병모 國立 昌原大學校 精報通信硏究所 1998 精報通信論文集 Vol.2 No.-

The current issues in the execution of Prolog programs are focused on WAM. Since intelligent backtracking which reduce nonfruitful or unnecessary work speeds up execution of Prolog, it should be incorporated intelligent backtracking in WAM. Intelligent backtracking, however, suffers from overhead to maintain a lot of information. The semi-intelligent method proposed by Chang and Despain has advantage of low overhead. We implement semi-intelligent backtracking on WAM. We extend machine architecture and change some instructions.

버섯 중독 환자에서의 ^(99m)Tc-galactosyl human serum albumin (GSA) scintigraphy 소견 : 간초음파 소견과의 비교 Comparison with Liver Ultrasonography

정신영,이재태,배진호,천경아,안병철,강영모,정재민,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.4

광대버섯과 같은 독버섯 중독시 간세포에 독성을 나타내어 간 기능의 이상을 유발할 수 있다. 저자들은 개나리광대버섯을 섭취한 3명의 환자에서 간 기능을 평가하기 위하여 간세포에 선택적으로 섭취되어 간기능을 평가하는데 사용되어지고 있는 99mTc-galactosyl human serum albumin (Tc-GSA)을 이용한 간 스캔을 시행하였고 간 초음파 검사와 비교하여 보았다. Tc-GSA 185MBq (3mg of GSA)을 정맥주사후 30분동안 동적 영상과 간과 심장 부위의 시간 방사능 곡선을 얻었다. 간기능의 정도를 평가하기 위하여 Tc-GSA의 간섭취 정도와 혈중 정체정도를 시각적인 평가와 간과 심장 부위의 시간 방사능 곡선을 이용한 반정량적인 평가를 시행하여 간섭취지수와 혈중제거지수를 구하였다. 시각적인 평가에서 2명의 환자에서 Tc-GSA의 경미한 정도의 간섭취 감소와 혈중 정체를 보였고 1명에서는 중등도의 간섭취 감소와 혈중 정체를 보였다. 반정량적인 평가에서는 2명의 환자에서 경미한 정도의 간섭취지수의 감소와 혈중제거지수의 증가를 보였고 1명에서 중등도의 간섭취지수의 감소와 혈중제거지수의 증가가 관찰되었다. 간초음파상에서는 1명의 환자는 지방침윤이 관찰되었으며 1명의 환자에서는 미약한 정도의 지방간이 관찰되어 급성 간기능 장애를 적절히 반영하지 못하였다. 본 증례를 통해 저자들은 간초음파 검사가 특이한 소견을 나타내지 못하여 간세포에 영향을 주는 독소나 약물 중독시에 간 영상화에는 Tc-GSA 간스캔이 유용하게 사용될 수 있을 것으로 추측할 수 있었다. ^(99m)Tc-galactosyl human serum albumin (Tc-GSA) is a radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically present in the hepatocyte membrane. Because these receptors are decreased in hepatic parenchymal damage, the degree of Tc-GSA accumulation in the liver correlates with findings of liver function test. Hepatic images were performed with Tc-GSA in patients with acute hepatic dysfunction by Amantia Subjunquillea poisoning, and compared with these of liver ultrasonography (USG). Tc-GSA (185 MBq, 3 mg of GSA) was injected intravenously, and dynamic images were recorded for 30minutes. Time-activity curves for the heart and liver were generated from regions of interest for the whole liver and precordium. Degree of hepatic uptake and clearance rate of Tc-GSA were generated by visual interpretation and semiquantitative analysis parameters (receptor index :LHL15 and index of blood clearance :HH15). Visual assessment of GSA scintigraphy revealed mildly decreased liver uptake in all of subjects. The mean LHL15 and HH15 were 0.886 and 0.621, graded as mild dysfunction in 2, and mild to moderate dysfunction in 1 subject. In contrast, liver USG showed no remarkable changes of hepatic parenchyme. Tc-GSA scintigraphy was considered as a useful imaging modality in the assessment of the hepatic dysfunction.

Inhibition of glutamine utilization sensitizes lung cancer cells to apigenin-induced apoptosis resulting from metabolic and oxidative stress

LEE, YOON-MI,LEE, GIBOK,OH, TAEK-IN,KIM, BYEONG MO,SHIM, DO-WAN,LEE, KWANG-HO,KIM, YOUNG JUN,LIM, BEONG OU,LIM, JI-HONG Spandidos Publications 2016 International journal of oncology Vol.48 No.1

<P>Recent studies have shown anticancer activity of apigenin by suppressing glucose transporter 1 (GLUT1) expression in cultured cancer cells; however, it is not clear whether apigenin can suppress glucose metabolism in lung cancer cells or sensitize them to inhibition of glutamine utilization-mediated apoptosis through metabolic and oxidative stress. We show that apigenin significantly decreases GLUT1 expression in mice. Furthermore, we demonstrate that apigenin induces growth retardation and apoptosis through metabolic and oxidative stress caused by suppression of glucose utilization in lung cancer cells. The underlying mechanisms were defined that the anticancer effects of apigenin were reversed by ectopic GLUT1 overexpression and galactose supplementation, through activation of pentose phosphate pathway-mediated NADPH generation. Importantly, we showed that severe metabolic stress using a glutaminase inhibitor, compound 968, was involved in the mechanism of sensitization by apigenin. Taken together, the combination of apigenin with inhibitors of glutamine metabolism may provide a promising therapeutic strategy for cancer treatment.</P>

Wireless powered wearable micro light-emitting diodes

Lee, Han Eol,Lee, Daewon,Lee, Tae-Ik,Shin, Jung Ho,Choi, Gwang-Mun,Kim, Cheolgyu,Lee, Seung Hyung,Lee, Jae Hee,Kim, Yong Ho,Kang, Seung-Mo,Park, Sang Hyun,Kang, Il-Suk,Kim, Taek-Soo,Bae, Byeong-Soo,Le Elsevier 2019 Nano energy Vol.55 No.-

<P><B>Abstract</B></P> <P>Wearable displays are considered as a bilateral communication tool in the hyperconnected era. Although several electronic clothing display was demonstrated, high power consumption issue still remained. Here, we present wireless powered wearable μLEDs (WμLEDs) with excellent stability. A custom-designed wireless power supply system operated a 30 × 30 WμLED array on a fabric. The WμLED stability was intensively investigated under bending, stretching, 85 °C/85% relative humidity and artificial sunlight conditions, showing the suitability of WμLED outdoor usage. Finally, a passive-matrix WμLED display successfully emitted a brilliant red glow on a fabric.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Wireless powered wearable μLEDs (WμLEDs) with excellent stability was realized by a custom-designed wireless energy supply system on a fabric. </LI> <LI> WμLED stability was investigated under bending, stretching, 85 °C/85% relative humidity and artificial sunlight conditions, showing the suitability of WμLED outdoor usage. </LI> <LI> A passive-matrix WμLED display successfully emitted a brilliant red glow on a fabric. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Characteristics of Isotopic and Elemental Compositions of Potential Water Pollution Sources in Rural Area

Byeong-Jun Jeon,Se-In Lee,Sang-Sun Lim,Kwang-Seung Lee,Hyun-Jin Park,Jong-Hyun Ham,Kwang-Sik Yoon,Sang-Mo Lee,Woo-Jung Choi 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

APE1/Ref-1 Inhibits Phosphate-Induced Calcification and Osteoblastic Phenotype Changes in Vascular Smooth Muscle Cells

Lee, Ki Mo,Lee, Eun Ok,Lee, Yu Ran,Joo, Hee Kyoung,Park, Myoung Soo,Kim, Cuk-Seong,Choi, Sunga,Jeong, Jin-Ok,Jeon, Byeong Hwa MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.10

<P>Vascular calcification plays a role in the pathogenesis of atherosclerosis, diabetes, and chronic kidney disease; however, the role of apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) in inorganic phosphate (Pi)-induced vascular smooth muscle cell (VSMC) calcification remains unknown. In this study, we investigated the possible role of APE1/Ref-1 in Pi-induced VSMC calcification. We observed that Pi decreased endogenous APE1/Ref-1 expression and promoter activity in VSMCs, and that adenoviral overexpression of APE1/Ref-1 inhibited Pi-induced calcification in VSMCs and in an ex vivo organ culture of a rat aorta. However, a redox mutant of APE1/Ref-1(C65A/C93A) did not reduce Pi-induced calcification in VSMCs, suggesting APE1/Ref-1-mediated redox function against vascular calcification. Additionally, APE1/Ref-1 overexpression inhibited Pi-induced intracellular and mitochondrial reactive oxygen species production, and APE1/Ref-1 overexpression resulted in decreased Pi-induced lactate dehydrogenase activity, pro-apoptotic Bax levels, and increased anti-apoptotic Bcl-2 protein levels. Furthermore, APE1/Ref-1 inhibited Pi-induced osteoblastic differentiation associated with alkaline phosphatase activity and inhibited Pi-exposure-induced loss of the smooth muscle phenotype. Our findings provided valuable insights into the redox function of APE1/Ref-1 in preventing Pi-induced VSMC calcification by inhibiting oxidative stress and osteoblastic differentiation, resulting in prevention of altered osteoblastic phenotypes in VSMCs.</P>

Teratological Studies of Ginkgo biloba Extract(EGb 761) in Rabbits

Lee, Yong-Soon,Nam, Jeong-Seok,Che, Jeong-Hwan,Lee, Suk-Man,Yang, Jae-Man,Kang, Byeong-Cheol,Lee, Hak-Mo,Park, Jae-Hak,Kim, Dai-Yong,Kang, Sung-An Korean Society of ToxicologyKorea Environmental Mu 1996 Toxicological Research Vol.12 No.1

A teratological study was performed using New Zealand White rabbits to examine the teratological potential of Ginkgo biloba extract(EGb 761), which is a known strong platelet activating factor antagonist. Ginkgo biloba extract(EGb 761) was administered per intravenously during the organogenesis period (day 6th to 18th of gestation) of rabbits at dose levels of 7.5, 15, and 30 mg/kg/day. All pregnant females were sacrificed on day 29 of gestation and teratological abnormalities of their fetuses was examined. No statistically significant difference of body weight change between control and treated groups during experimental periods was noted. There was no statistically signifiant difference of numbers of corpus lutes and implantations, fetal death ratio, fetal sex ratio, and placental weight between control and rabbits exposed to three different concentration ranges of Ginkgo biloba extract (EGb 761). No marked external, visceral and skeletal abnormalities related to Ginkgo biloba extract(EGb 761) were observed in the fetuses. In conclusion Ginkgo biloba extract(EGb 761) does not show any effect on implantation or embryonic development.