Laccase Production Using Pleurotus ostreatus 1804 Immobilized on PUF Cubes in Batch and Packed Bed Reactors: Influence of Culture Conditions

Prasad K. Krishna,Mohan S. Venkata,Bhaskar Y. Vijaya,Ramanaiah S. V.,Babu V. Lalit,Pati B. R.,Sarma P. N. The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.3

The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, $Cu^{2+}$ concentration, and alcohol nature, showed significant influence on the laccase yield. Gel electrophoretic analysis showed the molecular weight of the laccase produced by immobilized P. ostreatus to be 66 kDa. The laccase yield was significantly higher and more rapid in the packed bed reactor than in the shake flask experiments. A maximum laccase yield of 392.9 U was observed within 144 h of the fermentation period with complete glucose depletion.

Laccase Production Using Pleurotus ostreatus 1804 Immobilized on PUFCubes in Batch and Packed Bed Reactors: Influence of Culture Conditions

K. Krishna Prasad,S. Venkata Mohan,Y. Vijaya Bhaskar,S. V. Ramanaiah,V. Lalit Babu,B. R. Pati,P. N. Sarma 한국미생물학회 2005 The journal of microbiology Vol.43 No.3

The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, Cu2+ concentration, and alcohol nature, showed significant influence on the laccase yield. Gel electrophoretic analysis showed the molecular weight of the laccase produced by immobilized P. ostreatus to be 66 kDa. The laccase yield was significantly higher and more rapid in the packed bed reactor than in the shake flask experiments. A maximum laccase yield of 392.9 U was observed within 144 h of the fermentation period with complete glucose depletion.

Incidentally Detected Adenocarcinoma Prostate in Transurethral Resection of Prostate Specimens: a Hospital Based Study from India

Varghese, Jophy,Kuruvilla, Priya Mariam,Mehta, Nisarg,Rathore, Ranjeet Singh,Babu, Manas,Bansal, Devesh,Pillai, Biju,Sam, Mohan P,Krishnamorthy, H Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Background: Awareness about prostate cancer has increased in the community, and prostate cancer screening examinations, including prostate specific antigen (PSA) assays, are now widely available. Prior to the PSA era, up to 27% of prostate cancers were detected incidentally at the time of transurethral resection of prostate (TURP). After PSA testing became widely available, the incidence of incidentally detected carcinoma prostate in TURP specimens without prior diagnosis reduced to 5-13%. However, the incidence of incidentally detected carcinoma prostate has been reported to vary across the globe since various factors can influence the identification of this malignancy in TURP specimens. In this paper, we focus on rates of incidentally detected prostate cancer in TURP specimens in our hospital and correlate it with various parameters. Materials and Methods: This retrospective study of histopathological findings of biopsy specimens was conducted for patients undergoing TURP during a period of 5 years from April 2010. The inclusion criteria were patients diagnosed with benign prostatic hyperplasia (BPH) (digital rectal examination (DRE) not showing any abnormally hard areas and normal age adjusted PSA values). Patients with elevated PSA, abnormal DRE, documented urinary tract infection and proved adenocarcinoma prostate (CaP) were excluded from the study. The total weight of prostatectomy specimen, occurrence of carcinoma prostate in the chips, percentage of total tissue resected showing malignancy and Gleason's scores were recorded. Results: A total of 597 patients belonging to the inclusion criteria were studied. The incidence of occult CaP in the study group was 5.2 % (31/597). Out of these, 8 belonged to T1a and 23 belonged to T1b stages. The age group 70 - 79 years had the maximum incidence of occult CaP. It was observed that the clinical grading of prostate did not have a bearing on the incidence of occult CaP whereas the weight of resected specimen correlated with the incidence of CaP. The incidence of occult CaP was greater with low volume prostates (<20 g). (P=0.15). Conclusions: The rate of incidentally detected adenocarcinoma prostate in patients undergoing TURP for clinically diagnosed BPH was found to be only 5.2 % in our study which is low when compared with similar studies done elsewhere. The age of the patient and weight of the resected specimen correlated with incidence of occult prostate cancer. The clinical grading of prostate by DRE however, demonstrated no correlation.

Rational design of Cu-doped Co3O4@carbon nanocomposite and agriculture crop-waste derived activated carbon for high-performance hybrid supercapacitors

Mohan Reddy Pallavolu,Kurugundla Gopi Krishna,Goli Nagaraju,P.S. Srinivasa Babu,Sangaraju Sambasivam,ADEM SREEDHAR 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-

Development of structurally stable transition metal-oxides and cost-effective biomass-based carbon materials have attracted considerable attention in the fabrication of hybrid supercapacitors. In this work, we designed spinal copper-doped cobalt oxide (Cu-Co3O4 ) nanoboxes decorated functionalized-carbon nanotubes (f-CNTs) as hybrid redox-type material and agriculture crop-waste derived mesoporous activated carbon as capacitive-type electrode for high-performance hybrid supercapacitors. Structural properties reveal that the Cu-Co3O4 has a cubic spinel structure and Raman spectra results confirm the presence of f-CNTs. The hybrid composite material demonstrates superior redox behavior with excellent structural durability. The hybrid electrodes exhibit maximum specific capacity of 130.7 mAh g−1 at 0.5 A g−1 with 86.7 % capacitance retention over 10,000 cycles. Besides, the crop waste-derived activated carbon demonstrates high surface area (1549 m2g-1), mesoporous characteristics and excellent capacitive behavior. The high voltage hybrid supercapacitor is further fabricated with Cu-Co3O4 @F-CNTs as battery-type and biomass-derived activated carbon as capacitive-type electrodes, which demonstrate high energy density of 30.8 Wh kg−1 at 5972 W kg -1 power density. The augmented results indicate that the hybrid composites with biomass-derived carbon materials pave the way for design of eco-friendly energy storage applications.

Examination and modification of equivalent delamination factor for assessment of high speed drilling

J. Babu,Nevin Paul Alex,Keerthy P. Mohan,Jose Philip,J. P. Davim 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.11

Composite components suffer delamination at the entrance and exit of drilled holes. Many measures have been suggested by different researchers to assess such delamination damage. These include delamination factor, two-dimensional delamination factor, damage ratio, adjusted delamination factor, refined delamination factor, equivalent delamination factor, and minimum delamination factor. Among all these various assessment factors, the equivalent delamination factor looks simple and able to take into account the different features of delamination. However, the method of calculation of the equivalent delamination factor may not provide accurate values for delamination resulting from high speed drilling. The aim of this paper is to evaluate the equivalent delamination factor in high speed drilling of a composite laminate using a twist drill and develop a new approach to determine equivalent delamination factor which can be used for both conventional and high speed drilling conditions. This new method is applied to calculate the equivalent delamination factor in trials of drilling composite specimens at different speeds and feed rates and is found suitable.

Multiparty Handoff Mechanism in Mobile Ipv4 Networks

K. N. Rama Mohan Babu,K. N. Balasubramanya Murthy,Ram P. Rustagi 보안공학연구지원센터 2012 International Journal of Future Generation Communi Vol.5 No.1

Effcacy and Safety of Lumen-Apposing Stents for Management of Pancreatic Fluid Collections in a Community Hospital Setting

Rajat Garg,Abdelkader Chaar,Susan Szpunar,Babu P. Mohan,Mohammed Barawi 대한소화기내시경학회 2020 Clinical Endoscopy Vol.53 No.4

Background/Aims: Endoscopic ultrasound-guided transmural drainage and necrosectomy employing lumen-apposing metal stent(LAMS) are used for treating pancreatic fluid collections (PFCs) with excellent results from academic centers. Herein, we report theeffcacy and safety of LAMS in the treatment of PFCs at a community hospital. Methods: We retrospectively reviewed the etiology of pancreatitis, type and size of PFCs, length of procedure, technical success,clinical success, adverse events, and stent removal. The primary outcome was the rate of clinical success, and secondary outcomes weretechnical success and adverse events. Results: Twenty-seven patients with a mean age of 54.1±6.5 years were included, 44% of which were men. The mean size of the PFCswas 9.7±5.0 cm (range, 3–21). The most common etiology of pancreatitis was alcohol (44%) followed by idiopathic causes (30%) andpresence of gallstones (22%). The diagnosis was pseudocyst in 44.4% (12/27) and walled off necrosis in 55.6% (15/27) of patients. Therewas 100% technical success without any complications. Clinical success was achieved in 22 of 27 patients (81.5%) who underwent stentremoval. Conclusions: Our study is the first to report that endoscopic therapy of PFCs using LAMS is safe and effective even in a communityhospital setting with limited resources and support compared to large academic centers.

Underwater Endoscopic Mucosal Resection for 10 mm or Larger Nonpedunculated Colorectal Polyps: A Systematic Review and Meta-Analysis

Rajat Garg,Amandeep Singh,Manik Aggarwal,Jaideep Bhalla,Babu P. Mohan,Carol Burke,Tarun Rustagi,Prabhleen Chahal 대한소화기내시경학회 2021 Clinical Endoscopy Vol.54 No.3

Background/Aims: Recent studies have reported the favorable outcomes of underwater endoscopic mucosal resection (UEMR)for colorectal polyps. We performed a systematic review and meta-analysis evaluating the efficacy and safety of UEMR fornonpedunculated polyps ≥10 mm. Methods: We performed a comprehensive search of multiple databases (through May 2020) to identify studies reporting theoutcomes of UEMR for ≥10 mm nonpedunculated colorectal polyps. The assessed outcomes were recurrence rate on the first followup,en bloc resection, incomplete resection, and adverse events after UEMR. Results: A total of 1276 polyps from 16 articles were included in our study. The recurrence rate was 7.3% (95% confidence interval[CI], 4.3–12) and 5.9% (95% CI, 3.6–9.4) for nonpedunculated polyps ≥10 and ≥20 mm, respectively. For nonpedunculated polyps≥10 mm, the en bloc resection, R0 resection, and incomplete resection rates were 57.7% (95% CI, 42.4–71.6), 58.9% (95% CI, 42.4–73.6), and 1.5% (95% CI, 0.8–2.6), respectively. The rates of pooled adverse events, intraprocedural bleeding, and delayed bleedingwere 7.0%, 5.4%, and 2.9%, respectively. The rate of perforation and postpolypectomy syndrome was 0.8%. Conclusions: Our systematic review and meta-analysis demonstrates that UEMR for nonpedunculated colorectal polyps ≥10 mm issafe and effective with a low rate of recurrence.