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RISS 인기검색어
- 검색키워드
- 저자 : Mohan S. Venkata (검색결과 3 건)
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Prasad K. Krishna,Mohan S. Venkata,Bhaskar Y. Vijaya,Ramanaiah S. V.,Babu V. Lalit,Pati B. R.,Sarma P. N. The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.3
The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, $Cu^{2+}$ concentration, and alcohol nature, showed significant influence on the laccase yield. Gel electrophoretic analysis showed the molecular weight of the laccase produced by immobilized P. ostreatus to be 66 kDa. The laccase yield was significantly higher and more rapid in the packed bed reactor than in the shake flask experiments. A maximum laccase yield of 392.9 U was observed within 144 h of the fermentation period with complete glucose depletion.
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K. Krishna Prasad,S. Venkata Mohan,Y. Vijaya Bhaskar,S. V. Ramanaiah,V. Lalit Babu,B. R. Pati,P. N. Sarma 한국미생물학회 2005 The journal of microbiology Vol.43 No.3
The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, Cu2+ concentration, and alcohol nature, showed significant influence on the laccase yield. Gel electrophoretic analysis showed the molecular weight of the laccase produced by immobilized P. ostreatus to be 66 kDa. The laccase yield was significantly higher and more rapid in the packed bed reactor than in the shake flask experiments. A maximum laccase yield of 392.9 U was observed within 144 h of the fermentation period with complete glucose depletion.
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Naresh Kumar, A.,Min, Booki,Venkata Mohan, S. Elsevier 2018 Bioresource Technology Vol.269 No.-
<P><B>Abstract</B></P> <P>The objective of the study was to evaluate the potential application of defatted algal biomass (DAB) residue as a resource for biobased product synthesis in the biorefinery framework. Acid-catalyzed pretreatment of DAB residue resulted in higher reducing sugars (RS) solubilization (0.26 g RS/g DAB) than corresponding base method (0.19 g RS/g DAB). Subsequently, resulting RS were acidogenically fermented for the production of Bio-H<SUB>2</SUB> and short chain carboxylic acids (SCA)/volatile fatty acids (VFA) at varying redox conditions (pH: 6, 7 and 10). Biosystem with pH-6 resulted in higher SCA (0.54 g SCA/g RS) and Bio-H<SUB>2</SUB> production (0.83 l) followed by pH-10 (0.43 g SCA/g RS, 0.71 l) and pH-7 (0.27 g SCA/g RS, 0.48 l). Higher SCA production in pH-6 system resulted in maximum acidification (23%). Algal biomass majorly derived from CO<SUB>2</SUB> and its residues after lipids extraction accounted as major feedstock for acidogenic product synthesis. Evaluation of these studies using DAB residues offers sustainability to algal refineries on its entirety use.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Acid catalyzed pretreatment of DAB residues resulted in higher sugar solubilization. </LI> <LI> Higher biohydrogen production was resulted with pH-6 operation. </LI> <LI> Biosystem redox condition showed influence on SCA production and distribution. </LI> <LI> Use of DAB as a resource renders the sustainability to current algal biorefineries. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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