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Muhammad Waqas,Muhammad Tehseen Azhar,Iqrar Ahmad Rana,Farrukh Azeem,Muhammad Amjad Ali,Muhammad Amjad Nawaz,정규화,Rana Muhammad Atif 한국유전학회 2019 Genes & Genomics Vol.41 No.4
Background WRKY proteins play a vital role in the regulation of several imperative plant metabolic processes and pathways, especially under biotic and abiotic stresses. Although WRKY genes have been characterized in various major crop plants, their identification and characterization in pulse legumes is still in its infancy. Chickpea (Cicer arietinum L.) is the most important pulse legume grown in arid and semi-arid tropics. Objective In silico identification and characterization of WRKY transcription factor-encoding genes in chickpea genome. Methods For this purpose, a systematic genome-wide analysis was carried out to identify the non-redundant WRKY transcription factors in the chickpea genome. Results We have computationally identified 70 WRKY-encoding non-redundant genes which were randomly distributed on all the chickpea chromosomes except chromosome 8. The evolutionary phylogenetic analysis classified the WRKY proteins into three major groups (I, II and III) and seven sub-groups (IN, IC, IIa, IIb, IIc, IId and IIe). The gene structure analysis revealed the presence of 2–7 introns among the family members. Along with the presence of absolutely conserved signatory WRKY domain, 19 different domains were also found to be conserved in a group-specific manner. Insights of gene duplication analysis revealed the predominant role of segmental duplications for the expansion of WRKY genes in chickpea. Purifying selection seems to be operated during the evolution and expansion of paralogous WRKY genes. The transcriptome data-based in silico expression analysis revealed the differential expression of CarWRKY genes in root and shoot tissues under salt, drought, and cold stress conditions. Moreover, some of these genes showed identical expression pattern under these stresses, revealing the possibility of involvement of these genes in conserved abiotic stress–response pathways. Conclusion This genome-wide computational analysis will serve as a base to accelerate the functional characterization of WRKY TFs especially under biotic and abiotic stresses.
Mariyam,Muhammad Shafiq,Muhammad Haseeb,Rana Muhammad Atif,Syed Agha Armaghan Asad Abbas Naqvi,Numan Ali,Muhammad Arshad Javed,Fizza Gillani,Muhammad Saleem Haider 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.6
DNA binding with one fi nger (Dof) proteins are encoded by a ubiquitous plant-specifi c transcription factor gene family thatplays a critical role in various biological processes including fruit ripening and organogenesis. The wild olive ( Olea europaeavar. sylvestris v1.0 ) genome was used to identify Dof gene family members using a set of bioinformatics tools. Gene structure,chromosome locations, phylogeny, protein motifs, miRNA targets and tissue-specifi c expression patterns were analyzed. Here, we identifi ed 51 potential Dof genes unevenly distributed on all chromosomes and a few scaff olds. Dof proteins in oliveclustered into eight subgroups (D1, B2, C3, C2.2, C1, C2.1, B1, and A) based on the established Arabidopsis classifi cation. The prevalence of segmental duplication was observed as compared to tandem duplication, and this was the main factorunderlying the expansion of the Dof gene family in olive. Tissue-specifi c expression profi ling of Oeu Dof genes revealed thatthe majority of Oeu Dof genes were highly expressed in fl owers, stem and meristem tissues. In seed and meristem tissues,cis-regulatory element (CRE) analysis revealed the presence of elements that are specifi cally responsive to light, circadian,auxin, and ABA. In addition, a comparative analysis between Dof genes in olive and Arabidopsis revealed eight groups orsub-families, although the C3 group of Arabidopsis was not represented in olive. This extensive genome evaluation of theDof gene family in olive presents a reference for cloning and functional analysis of the members of this gene family.
COVID-Beat: a low-cost breath monitoring approach for people in quarantine during the pandemic
Atif Muhammad,Muralidharan Shapna,Ko Heedong,Yoo Byounghyun 한국CDE학회 2022 Journal of computational design and engineering Vol.9 No.3
Due to COVID-19, people have to adapt to the new lifestyle until scientists develop a permanent solution for this pandemic. Monitoring the respiration rate is very important for a COVID-infected person because the Coronavirus infects the pulmonary system of the person. Two problems that arise while monitoring the breath rate are: sensors are contact based and expensive for mass deployment. A conventional wearable breath rate monitoring system burdens the COVID-affected patient and exposes the caregivers to possible transmission. A contactless low-cost breath monitoring system is required, which monitors and records the breath rate continuously. This paper proposes a breath rate monitoring system called COVID-Beat, a wireless, low-cost, and contactless Wi-Fi-based continuous breath monitoring system. This sensor is developed using off-the-shelf commonly available embedded Internet of Thing device ESP32, and the performance is validated by conducting extensive experimentation. The breath rate is estimated by extracting the channel state information of the subcarriers. The system estimates the breath rate with a maximum accuracy of 99% and a minimum accuracy of 91%, achieved by advanced subcarrier selection and fusion method. The experimental results show superior performance over the existing breath rate monitoring technologies.
Wi-ESP—A tool for CSI-based Device-Free Wi-Fi Sensing (DFWS)
Muhammad Atif,Shapna Muralidharan,Heedong Ko,Byounghyun Yoo 한국CDE학회 2020 Journal of computational design and engineering Vol.7 No.5
Recent progress in Device-Free Wi-Fi Sensing (DFWS) has established the use of wireless signals like Wi-Fi not only to communicate but also as a tool to enable device-free sensing. As an emerging technique, DFWS has many capable applications such as sensing activity and gesture and fall detection, monitoring elderly, surveillance, and many more applications while waiving out the necessity to mount devices on the object. A wide range of applications can use the channel state information (CSI) from commercial Wi-Fi devices pervasively for ubiquitous sensing. Existing CSI tools, such as the Intel 5300 network interface controller tool or the Atheros 9390 tool, have limitations when deployed in large-scale systems due to their high deployment costs and limitations in the resolution of CSI measurements. Due to these shortcomings, DFWS applications need an alternative CSI tool in order to perform efficiently. In this paper, we present ESP32-based Wi-ESP as a CSI gathering tool that can report detailed CSI measurements based on 802.11n standards. The proposed Wi-ESP tool works as a complete device by collecting the CSI measurements as well as processing further for DFWS applications. Wi-ESP can work as standalone device, unlike other CSI tools, and can offer large-scale deployment to many DFWS applications. In this paper, we have explored the options of Wi-ESP as a tool for CSI measurements and processing and propose it as a tool for DFWS.
Muhammad Asif Hussain,최은주,Adnan Maqbool,Muhammad Atif,Hassan Zeb,여재구,유정아,조영훈,Moses Noh,김정원 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-
A facile eco-friendly heterogeneous catalytic system has been developed for amide synthesis that furtherutilized in pharmaceutical and organic chemistry. The Ru/MnO2 catalyst has shown outstanding andunprecedented activity for a wide range of aliphatic and benzylic nitriles in green solvent water at 60 C. The system has also exhibited a remarkable tolerance for selective hydration of heteroatom (e.g. nitrogen,oxygen and sulphur atoms) containing nitriles. Pharmaceutically important nicotinamides andpyrazinamide has been synthesized by hydration of the heteroatomic nitriles with appreciable yieldsand selectivities. Moreover, the Ru/MnO2 catalyst has employed water as a benign solvent, with morethan 30,000 TONs and reusabilityfive times after isolation from the reaction mixture by centrifugationand easy workup that established a path for green environmental and technologically acceptableprotocol.
Muhammad Rizwan Dilshad,Atif Islam,Aneela Sabir,Muhammad Shafiq,Muhammad Taqi Zahid Butt,Aamir Ijaz,Tahir Jamil 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.55 No.-
In this study zinc oxide filled and unfilled polyvinyl alcohol (PVA)/polyethylene glycol (PEG) 600 blended membranes with different degrees of cross-linking (mol%) were prepared using formaldehyde as a crosslinking agent and characterized by FTIR, SEM, TGA, mechanical analysis and permeation testing for CO2 and N2 gases. The effects of synthesis parameters like cross-linking ratio, wt.% PEG content and wt.% zinc oxide content on the membrane characteristics were also investigated. It was found that at 60 mol% degree of cross-linking and 60 wt.% PEG content, the membranes showed maximum CO2/N2 ideal selectivity of 26 and reasonable CO2 permeability of 52.9 Barrers at 25 C and 1.5 bar. It was also found that at 2 wt.% zinc oxide content, membranes showed highest CO2/N2 ideal selectivity of 112.8 and maximum CO2 permeability of 423 Barrers at 25 C and 1.5 bar.