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Muhammad Waqas,Muhammad Tehseen Azhar,Iqrar Ahmad Rana,Farrukh Azeem,Muhammad Amjad Ali,Muhammad Amjad Nawaz,정규화,Rana Muhammad Atif 한국유전학회 2019 Genes & Genomics Vol.41 No.4
Background WRKY proteins play a vital role in the regulation of several imperative plant metabolic processes and pathways, especially under biotic and abiotic stresses. Although WRKY genes have been characterized in various major crop plants, their identification and characterization in pulse legumes is still in its infancy. Chickpea (Cicer arietinum L.) is the most important pulse legume grown in arid and semi-arid tropics. Objective In silico identification and characterization of WRKY transcription factor-encoding genes in chickpea genome. Methods For this purpose, a systematic genome-wide analysis was carried out to identify the non-redundant WRKY transcription factors in the chickpea genome. Results We have computationally identified 70 WRKY-encoding non-redundant genes which were randomly distributed on all the chickpea chromosomes except chromosome 8. The evolutionary phylogenetic analysis classified the WRKY proteins into three major groups (I, II and III) and seven sub-groups (IN, IC, IIa, IIb, IIc, IId and IIe). The gene structure analysis revealed the presence of 2–7 introns among the family members. Along with the presence of absolutely conserved signatory WRKY domain, 19 different domains were also found to be conserved in a group-specific manner. Insights of gene duplication analysis revealed the predominant role of segmental duplications for the expansion of WRKY genes in chickpea. Purifying selection seems to be operated during the evolution and expansion of paralogous WRKY genes. The transcriptome data-based in silico expression analysis revealed the differential expression of CarWRKY genes in root and shoot tissues under salt, drought, and cold stress conditions. Moreover, some of these genes showed identical expression pattern under these stresses, revealing the possibility of involvement of these genes in conserved abiotic stress–response pathways. Conclusion This genome-wide computational analysis will serve as a base to accelerate the functional characterization of WRKY TFs especially under biotic and abiotic stresses.
Muhammad Waqas,Imad-ud-din Saqib,Muhammad Imran Jamil,Mohammad Ayaz Khan,Saeed Akhter 대한비뇨의학회 2018 Investigative and Clinical Urology Vol.59 No.1
Purpose: To evaluate the impact of various computed tomography scan-based parameters of renal stones on the outcome of extracorporeal shock wave lithotripsy (ESWL). Materials and Methods: We conducted a retrospective study of patients who underwent ESWL for renal stones (sized 5–20 mm) from January 2013 to December 2016. We evaluated body mass index, location of the stone, skin-to-stone distance (SSD), stone attenuation value (SAV), stone diameter, Hounsfield density, stone area, and stone volume. Statistical analysis was done and significance was confirmed by multivariate logistic regression analysis. Results: Of the 203 patients 122 (60.1%) had successful clearance of the stone. The presence of a double J stenting, a lower pole location, a higher SAV, higher Hounsfield density, larger stone area, larger stone diameter, and higher stone volume were negative predictors of ESWL outcome. When these parameters were analyzed with multivariate logistic regression analysis, stone location, SSD, and SAV were the only significant independent predictors of the outcome of ESWL. Conclusions: Stone location, SSD, and SAV are reliable and strong predictors of ESWL outcome for the treatment of renal stones.
( Muhammad Waqas ),( Huyen Trang Trinh ),( Sungeun Lee ),( Dae-hwan Kim ),( Sang Yeol Lee ),( Kevin K. Choe ),( Chongsuk Ryou ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
Based on previous studies reporting the anti-prion activity of poly-L-lysine and poly-Larginine, we investigated cationic poly-L-ornithine (PLO), poly-L-histidine (PLH), anionic poly-L-glutamic acid (PLE) and uncharged poly-L-threonine (PLT) in cultured cells chronically infected by prions to determine their anti-prion efficacy. While PLE and PLT did not alter the level of PrP<sup>Sc</sup>, PLO and PLH exhibited potent PrP<sup>Sc</sup> inhibition in ScN2a cells. These results suggest that the anti-prion activity of poly-basic amino acids is correlated with the cationicity of their functional groups. Comparison of anti-prion activity of PLO and PLH proposes that the anti-prion activity of poly-basic amino acids is associated with their acidic cellular compartments.
Waqas, Muhammad,Jeong, Woo-jin,Lee, Young-Joo,Kim, Dae-Hwan,Ryou, Chongsuk,Lim, Yong-beom AMERICAN CHEMICAL SOCIETY 2017 Biomacromolecules Vol. No.
<P>The first step in the conventional approach to self-assembled biomaterials is to develop well-defined nano structures in vitro, which is followed by disruption of the preformed nanostructures at the inside of the cell to achieve bioactivity. Here, we propose an inverse strategy to develop in-cell gain-of-function self-assembled nanostructures. In this approach, the supramolecular building blocks exist in a unimolecular/unordered state in vitro or at the outside of the cell and assemble into well-defined nanostructures after cell internalization. We used block copolypeptides of an oligoarginine and a self-assembling peptide as building blocks and investigated correlations among the nanostructural state, antiprion bioactivity, and cytotoxicity. The optimal bioactivity (i.e., the highest antiprion activity and lowest cytotoxicity) was obtained when the building blocks existed In a unimolecular/unordered state in vitro and during the cell internalization process, exerting minimal cytotoxic damage to cell membranes, and were subsequently converted into high-charge-density vesicles in the low pH endosome/lysosomes in vivo, thus, resulting in the significantly enhanced antiprion activity. In particular, the in-cell self assembly concept presents a feasible approach to developing therapeutics against protein misfolding diseases. In general, the in cell self-assembly provides a novel inverse methodology to supramolecular bionanomaterials.</P>
Socio-Economic Factors and Women’s Empowerment: Evidence from Punjab, Pakistan
Muhammad Waqas Khalid,Nahla Samargandi,Aadil Hameed Shah,Seita Almandeel 한국국제경제학회 2020 International Economic Journal Vol.34 No.1
The empowerment ofwomenis an essential objective to fully engage them in economic life and achieve sustainable growth throughout the world. Providing basic facilities to women is one form of empowerment. This paper examines the extent of women’s empowerment in Punjab, Pakistan and its divisions, along with rural and urban regions. In addition, we check the effect of the gender wage differential on the current dilemma by implementing Alkire et al.’s [2013.The women’s empowerment in agriculture index (Working Paper No. 58). Oxford, UK: Oxford Poverty and Human Development Initiative. Retrieved from https://www.ophi.org.uk/wp-content/uploads/ophiwp- 58.pdf.] indexing on HIES 2013–14 datasets. Our results show that 34.91% of women are empowered in Punjab overall, with independence being the highest dimensional contributor, and ownership of assets being the least. Women are 31.43% more empowered in urban regions. The results indicate that Islamabad has significantly more women’s empowerment, while Dera Ghazi Khan has the lowest percentage of empowered women. To assess particular impacts of different socio-economic and demographic variables on women’s empowerment, logistic regression model is applied, revealing that most socio-economic and demographic variables have significant impacts on the current scenario, and variation in any variable causes significant variations in the status of women’s empowerment, with increased wage differential in particular, decreasing the probability of women being empowered.
Waqas Ahmad,Chaudhary Muhammad Ayyub,Muhammad Asif Shehzad,Khurram Ziaf,Muhammad Ijaz,Ahmad Sher,Tahira Abbas,Jamil Shafi 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.6
Soil salinity is one of the severe threats of climate change that inflicts heavy losses to vegetable production. Potassium (K) has been considered essential approach against abiotic stresses in food crops, however, understanding of K regulated mechanisms for inducing tolerance to NaCl stress in cabbage (Brassica oleracea L.) plants is, still elusive. Here, we report the supplemental K effects on antioxidant defense system and physiological processes that may influence the cabbage production under saline conditions. Initially, cabbage varieties (‘Stone Head’, ‘Golden Acre’, ‘9j-940’, ‘Beauty Ball’, ‘Green Ball’, ‘Green Rise’, ‘Marco F-1’) were tested under NaCl stress (50, 100, 150, and 200 mM) for their higher growth, vigor index and mineral contents. The identified cabbage var. salt-tolerant, cv. Beauty Ball (BB) and salt-sensitive cv. Green Ball (GB) were further exposed to foliar K (5 and 10 mM solutions of KNO3) under the same NaCl regimes. NaCl stress markedly inhibited photosynthetic efficiency, water status and chlorophyll pigments, thereby, resulted in reduced dry biomass of both varieties. Nevertheless, exogenous K spray at 10 mM caused positive gain in leaf water relations, chlorophyll contents in both cabbage varieties. The ameliorative impacts of K were more pronounced in salt-tolerant cv. BB as compared to salt-sensitive cv. GB in terms of higher accumulation of total soluble proteins, total free amino acids, proline contents, upregulated antioxidant activities and enhanced gas exchange characteristics. Hence, improvement in growth and K+/Na+ ratio of cabbage plants by foliar K application (10 mM) were related to up-regulation of physiological and biochemical mechanisms under saline conditions.
Coconut shell waste as an alternative lightweight aggregate in concrete- A review
Muhammad ,Aslam,Waqas, Aziz,M. Jahanzaib, Khalil,M. Jahanzaib, Ali,Muhammad, Raheel,Aayzaz, Ahmed,Muhammad Fahad, Ejaz Techno-Press 2022 Advances in materials research Vol.11 No.4
This review article highlights the physical, mechanical, and chemical properties of coconut shells, and the fresh and hardened properties of the coconut shell concrete are summarized and were compared with other types of aggregates. Furthermore, the structural behavior in terms of flexural, shear, and torsion was also highlighted, with other properties including shrinkage, elastic modulus, and permeability of the coconut shell concrete. Based on the reviewed literature, concrete containing coconut shell as coarse aggregate with normal sand as fine showed the 28-day compressive strength between 2 and 36 MPa with the dried density range of 1865 to 2300 kg/m<sup>3</sup>. Coconut shell concretes showed a 28-day modulus of rupture and splitting tensile strength values in the ranges of 2.59 to 8.45 MPa and 0.8 to 3.70 MPa, respectively, and these values were in the range of 5-20% of the compressive strength. The flexural behavior of CSC was found similar to other types of lightweight concrete. There were no horizontal cracks on beams which indicate no bond failure. Whereas, the diagonal shear failure was prominent in beams with no shear reinforcements while flexural failure mode was seen in beams having shear reinforcement. Under torsion, CSC beams behave like conventional concrete. Finally, future recommendations are also suggested in this study to investigate the innovative lightweight aggregate concrete based on the environmental and financial design factors.