Collagen II-induced Arthritis 생쥐에 대한 두충(杜仲).속단(續斷) 배합약물의 관절염 억제 효과

이부균,이영철,이장천,Lee, Boo-Kyun,Lee, Young-Cheol,Lee, Jang-Cheon 대한본초학회 2010 大韓本草學會誌 Vol.25 No.1

Objectives : The object of this study was to verify the inhibitory effect of a decoction of Eucommiae ulmides OLIVER (EU) and Dipsacus asperoides C. Y. Cheng et T.M.Ai (DA) on Collagen II-induced Arthritis Mice (CIA mice). Methods : DBA/1OlaHsd mice were immunized with bovine type II collagen. Boostnig same collagen 21 days later, arthritis was induced and then administrated orally the extract of EU+DA (200 or 50 mg/kg) once a day for 4 weeks and compared with that of methotrexate (MTX, 0.3 mg/kg) as a positive control. Results : Administration of EU+DA suppressed the inflammatory progression of CIA mice and the results were 1. Arthritis index of CIA mice was decreased. 2. EU+DA decreased the production of TNF-$\alpha$, IL-6, IL-$1{\beta}$ in the serum of CIA mice. 3. EU+DA decreased the level of IgM. 4. EU+DAincreasaed $CD3^+$, $CD4^+$, $CD4^+$/CD25 but decreased $CD19^+$, $CD3^+/CD49b^+$(NKT), $CD3^-/CD49b^+$(NK), $B220^+/CD23^+$ in PBMC of CIA mice. 5. EU+DA decreased $CD3^+$, $CD4^+$, $CD3^+/CD69^+$ of paw joint in CIA mice. 6. EU+DA decreased subsynovial inflammation. Conclusions : This results demonstrated that extract of EU+DA suppressed the inflammatory progression of CIA mice and supported further studies are required to clarify a mechanism of therapeutic role.

촉초(蜀椒)(천초(川椒)) 약대(藥對)에 관한 연구

이부균,임규상,강휘중,이장천,Lee, Boo-Kyun,Lim, Kyu-Sang,Kang, Hwi-Joong,Lee, Jang-Cheon 대한한의학방제학회 2012 大韓韓醫學方劑學會誌 Vol.20 No.1

Objectives : The purpose of this study is to find out herb-pair prototype composed of Zanthoxyli Fructus, to examine compatibility regulation between them and to classify them into seven features of compatibility. Methods : To search usages of Zanthoxyli Fructus compatibility, we have investigated the classics of herb and prescription such as "五十二病方"(Fifty-two kinds of Diseases), "黃帝內經"(The Medical Classic of Yellow Emperor), "傷寒論"(Treatise on Febrile Caused by Cold), "金?要略"(Synopsis of Prescriptions of The Golden), "附廣?后方"(Handbook of Prescriptions for Emergencies), "本草綱目"(Compendium of Materia Medica), "得配本草"(Deukbae Materia Medica). Results : In these books Zanthoxyli Fructus was paired with below herbs; 桂(Cinnamomi Cortex), 薑(Zingiberis Rhizoma), 吳茱萸(Evodiae Fructus), 細辛(Asari Radix), 茴香(Foeniculi Fructus), 肉荳?(Cistanches Herba), 釜下土(Terba Flava Usta), 益智仁(Alpinae Oxyphyllae Fructus), 飴(Saccharum Granorum), ?白(Allii Radix), 梨(Pyrus), 牙?(Gleditsia Fructus), 杏仁(Armeniacae Amarum Semen), 蕎麥粉(Fagopyrum esculentum Moench), 胡粉(Minium), 白朮(Atractylodis Rhizoma White), 蒼朮(Atractylodis Rhizoma), 麻子仁(Cannabis Fructus), 大麥蘗(Hordei Fructus Germiniatus), 麴(Massa Medicata Fermentata), 生地黃(Rehmanniae Radix), 茯?(Poria), 牡?(Ostreae Concha), 白礬(Alumen), 雄黃(Realgar), 烏頭(Aconiti Radix), 附子(Aconiti Lateralis Preparata Radix). Conclusions : Zanthoxyli Fructus herb-pair classified by seven features of compatibility are as follows; 1. Mutual reinforcement compatibility with : Cinnamomi Cortex, Zingiberis Rhizoma, Evodiae Fructus, Asari Radix, Foeniculi Fructus, Cistanches Herba, Terba Flava Usta, Alpinae Oxyphyllae Fructus, Saccharum Granorum, Allii Radix, Pyrus, Gleditsia Fructus. 2. Mutual assistance compatibility with : Armeniacae Amarum Semen, Fagopyrum esculentum Moench, Minium, Atractylodis Rhizoma White, Atractylodis Rhizoma, Cannabis Fructus, Hordei Fructus Germiniatus, Massa Medicata Fermentata, Rehmanniae Radix, Poria, Ostreae Concha, Alumen. 3. Mutual restraint compatibility with: Realgar, Aconiti Radix, Aconiti Lateralis Preparata Radix) 4. Mutual detoxication compatibility with: 桑汁(Juice of Mori Folium), 桑根汁(Juice of Morus Cortex), 大蒜(Allii Bulbus), 薺?(Remotiflori Radix), 桂汁(Juice of Cinnamomi Cortex).

『동의보감(東醫寶鑑)』중 식물성 기름(油)을 활용한 방제

이부균,Lee, Boo-Kyun 대한한의학방제학회 2020 大韓韓醫學方劑學會誌 Vol.28 No.3

Object : To investigate which diseases the utilization of various vegetable oils were used and how they were used for treatment, and to change and develop vegetable oil combined prescriptions so that the Korean medical treatment effect in clinical practice can be enhanced. Methods : All words containing oil(油) were searched and selected oils used for simple prescription and oil combined prescription. Aii of animal oils such as butter(酥油), Suis oil(猪油), Anceris oil(鴈油), and Actonis caro oil(猯油) were excludIt is suggested to develop a new formulation by succeeding the utilization methods of various effects of sesame oil and other vegetable oils.ed. All selected oils were classified as sesame oil(麻油)(inclusive, 脂麻油, 胡麻油, 香油, 淸油) or other vegetable oils. Result : 1. In 『Donguibogam』(『東醫寶鑑』), 14 other vegetable oils were used to 23 prescriptions. 2. Sesame oil was used to 187 prescriptions over 41 disease categories as of 20 effects described in the 『Zhenghebencao』(『政和本草』). 3. In 28 prescriptions, sesame oil was also used for hygiene and prevention, emetic, preparating herbs and so on. Conclusion : The 101 prescriptions used for medicinal auxiliary materials are due to the efficacy of treatments of malignant furuncles, sores, scabies, promoting regeneration of tissue and flesh, reliving pain, dispersing abscess and swelling and supplementing fissured skin. In 『Donguibogam』(『東醫寶鑑』), the unique efficacy of each vegetable oil and sesame oil was used in various ways and preparation forms to treat various diseases. It is suggested to develop a new formulation by succeeding the utilization methods of various effects of sesame oil and other vegetable oils.

동의보감(東醫寶鑑) 중 고마요법(膏摩療法)에 사용된 방제(方劑)에 대한 고찰(考察)

이부균,임강현,박성하,강휘중,이장천,Lee, Boo-Kyun,Leem, Kang-Hyun,Park, Seong-Ha,Kang, Hwi-Joong,Lee, Jang-Cheon 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.2

The purpose of this study is to expand the prescription usage from internal treatment to external treatment by applying the compatibilities of herbal ingredient for ointment massage therapy. For this, specific cases of usage were investigated in Donguibogam, which is now inscribed on the UNESCO Memory of the World Register, has been not only one of the classics of Korean medical asphere, but has compiled all the contents of the Oriental medical traditions including ointment massage therapy that was completed through different Chinese dynasties, such as Song (宋), Jin(金) and Won(元) by the 17th century. Research results of this study is : 1. Ointment Massage Therapy was used to treat the symptoms of diseases such as ACHI-mun(dental range : 牙齒門), PI-mun(dermal range : 皮門), JECHANG-mun (sore range : 諸瘡門), SO A-mun(pediatrics range : 小兒門). 2. Out of all the manipulations, it is a scrubbing manipulation that used most. 3. Out of all the formulations of ointment massage therapy, it is powder types and ointment types that most representative formulation.

동의보감(東醫寶鑑) 중(中) 소엽(蘇葉)이 주약(主藥)으로 배오(配伍)된 방제(方劑)의 활용(活用)에 대한 고찰(考察)

이부균,국윤범,이장천,Lee, Boo-Kyun,Kook, Yoon-Bum,Lee, Jang-Cheon 대한한의학방제학회 2005 大韓韓醫學方劑學會誌 Vol.13 No.1

This study was investigated to make sure the usage of prescriptions in which Perilla frutescens is used as a main herbal medicine in Donguibogam. Through the investigation on main treatment, pathology, etiology and nature of disease, dosage in the relevant prescriptions, The consequences are as follows; 1. The Perilla frutescens is used in 15 treatment fields which contain the cough, cold chapter ect. 2. The Perilla frutescens is used as a main heral medicine in 30 natures of disease such as cough-relative disease, an asthma, an external wind and cold, etc. 3. The Perilla frutescens is used for various pathology in the exogenous etiological factor, endogenous etiological factor, exo-endogeneous etiological factor and non-exo-endogeneous etiological factor. 4. The Perilla frutescens is used in a range of $1.2g{\sim}10g$ in relevant prescription. The main dosage is 4g. 5. The Perilla frutescens is used with various crude herbs in accordance with the pathogeny. The Perilla frutescens has been used to reduce heat from the lungs, to relieve asthma, to promote qi's movement and to soothe fetal movement etc. According to the results, 1 suggest to use the Perilla frutescens in a various pathogenic fields. The Perilla frutescens is able to remove not only pathogenic heat from the cough-relative disease, but also pathogenic asthma, an external winds and colds, etc.

Collagen 2-induced Arthritis 생쥐에 대한 두중(杜仲),속단(續斷) 배합약물의 관절염 억제 효과

이부균 ( Boo Kyun Lee ),이영철 ( Young Cheol Lee ),이장천 ( Jang Cheon Lee ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.1

Objectives: The object of this study was to verify the inhibitory effect of a decoction of Eucommiae ulmides OLIVER (EU) and Dipsacus asperoides C. Y. Cheng et T.M.Ai (DA) on Collagen II-induced Arthritis Mice (CIA mice). Methods: DBA/1OlaHsd mice were immunized with bovine type II collagen. Boostnig same collagen 21 days later, arthritis was induced and then administrated orally the extract of EU+DA (200 or 50 mg/kg) once a day for 4 weeks and compared with that of methotrexate (MTX, 0.3 mg/kg) as a positive control. Results: Administration of EU+DA suppressed the inflammatory progression of CIA mice and the results were 1. Arthritis index of CIA mice was decreased 2. EU+DA decreased the production of TNF-α, IL-6, IL-1β in the serum of CIA mice. 3. EU+DA decreased the level of IgM. 4. EU+DAincreasaed CD3+, CD4+, CD4+/CD25 but decreased CD19+, CD3+/CD49b+(NKT), CD3-/CD49b+(NK), B220+/CD23+ in PBMC of CIA mice. 5. EU+DA decreased CD3+, CD4+, CD3+/CD69+ of paw joint in CIA mice. 6. EU+DA decreased subsynovial inflammation. Conclusions: This results demonstrated that extract of EU+DA suppressed the inflammatory progression of CIA mice and supported further studies are required to clarify a mechanism of therapeutic role.

인체심장의 심부전모델에서의 獨蔘湯 투여에 따른 심장막전위 분석에 관한 연구

정대영,이부균,홍진우,안원근,Jeong, Dae-Yeong,Lee, Boo-Kyun,Hong, Jin-Woo,An, Won-Gun 대한한의학방제학회 2015 大韓韓醫學方劑學會誌 Vol.23 No.1

Objectives : This study was conducted to investigate the membrane potential to apply Doksam-tang to a human heart failure model. Methods : The human heart model was built by Luo et al. CellML model, Priebe et al. CellML model, and a human heart mesh file. Doksam-tang gives channel the half maximal inhibitory concentration(IC ₅₀ ), half maximal effective concentration(EC ₅₀ ) values and compounds concentrations. These data load into the laptop with Ubuntu OS, and build the library with the data. Results : While results of the study with the heart failure model shows abnormal membrane potential from the normal heart model, the study with applying Doksam-tang to heart failure model shows restoring membrane potential that is similar to normal heart model. Conclusions : These results of the testings suggest that a conception of novel technique to investigate the effects of Korean herbal medicine.

동의보감(東醫寶鑑) 중(中) 연교(連翹)가 주약(主藥)으로 배오(配伍)된 방제(方劑)의 활용(活用)에 대한 고찰(考察)

노의준,김창민,이부균,강성현,윤용갑,Rho Euy-Joon,Kim Chang-Min,Lee Boo-Kyun,Kang Sung-Hyun,Yun Young-Gab 대한한의학방제학회 2004 大韓韓醫學方劑學會誌 Vol.12 No.1

This report describes the studies as follows through the research into the data of Fructus Forsythiae main blended prescriptions from Dongeuybogam. Through investigation on prescriptions from Dongeuybogam with Fructus Forsythiae as an ingredient, the following conclusions were reached. 1. All kinds of infection in a boil and the malignant tumor recorded the largest number of clinical frequency of the prescriptions in therapeutic use when Fructus Forsythiae was taken as a principle medicine. 2. The prescriptions are compounded with Fructus Forsythiae as a principle medicine can apply to eye disease, nose and ear disease, throat disease, trauma, the malignant tumor, skin disease, dental disease and heat pathogenic disease. 3. Various pathogenic factors such as wind-heat, heat, fire, wind, qi, poison, cold are put to practical use. 4. The dosage of Fructus Forsythiae is 2bun(about 0.75 gram) to 3jeon(about 11.25 gram), however 1joen(about 3.75 gram) has been taken the most for clinical application. 5. With examination the formulae of presciptions containing Fructus Forsythiae, I found the basic prescriptions as well as the medicines are in conjunction with Fructus Forsythiae and the theoretic grounds of formula in combining Fructus Forsythiae as a group of medicine for prescription through this research.

『동의보감(東醫寶鑑)』 중 연제(烟劑)·훈제(熏劑)와 함금제(含?劑)의 활용(活用)에 관한 연구(硏究)

권재원,양윤홍,이부균,Kwon, Jae-Won,Yang, Yoon-Hong,Lee, Boo-Kyun 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.3

Objectives : The purpose of this study is to provide grounds for the development of preparation of Korean medicine through investigating the usage of smoking preparation, fumigant and buccal preparations in "Dongeuibogam". Methods : We have researched the usage of related preparations as following procedure : 1. Choosing a related words such as combustion(燒, 燎, 燃) smoking(烟), fumigation(熏, 熏烟, 熏洗), inhalation(聞, 嗅, ?), holding in mouth(含, ?). 2. Searching sentences : We have searched sentences containing the related words in "Dongeuibogam", analyzed the selected sentences and made tables of the usage including indication, formula, material. Conclusions : 1. Smoking preparations and Fumigant can be classified into two different groups : 1) internal usage for the treatment of internal diseases such as dizziness, congestion, stroke, cough. 2) external usage for the treatment of wounds, sores, genital diseases, anal disease and for the prevention of diseases. 2. Buccal preparation is mainly used for the treatment of mouth, tongue, throat and lung diseases. 3. These preparations have been used not only for external diseases but internal diseases. 4. Each preparation has chief herbs for its peculiar properties of treatment.

군신좌사 개념을 도입한 방제 검색 및 비교 시스템에 관한 연구

박한수,이병욱,이부균,Park, Hansu,Lee, ByungWook,Lee, Boo-Kyun 대한한의학방제학회 2014 大韓韓醫學方劑學會誌 Vol.22 No.2

Objectives : The main objective of this study is to classify herbal components to 4 groups which are similar to Gunsinjwasa grades by using herbal composition ratio of prescription. Another objective is to design the searching system which compares prescriptions and improves efficiency with 4 groups like Gunsinjwasa grades. Methods : This study was proceeded with Acess 2007 on Microsoft Windows 7 and we created composition ratio based on weight by using prescriptions of Donguibogam, Uihagipmun and Banghakhabpyun. We could make comparison and searching method of prescriptions. Results : We could search using composition ratio degree of herbs which composes prescription. And the similarity comparison of prescription was possible with value from 0 to 10. Conclusions : We could increase the accuracy of the searching prescriptions and comparison with putting into the information about composition degree and composition ratio of herbs which compose a prescription.