Anti-Cancer Effect of Ginsenoside F₂ against Glioblastoma Multiforme in Xenograft Model in SD Rats

Shin, Ji-Yon,Lee, Jung-Min,Shin, Heon-Sub,Park, Sang-Yong,Yang, Jung-Eun,KimCho, So-Mi,Yi, Tae-Hoo The Korean Society of Ginseng 2012 Journal of Ginseng Research Vol.36 No.1

The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside $F_2$ (F2) to treat GBM. In in vitro experiments with glioblastoma cells U373MG, F2 showed the cytotoxic effect with $IC_{50}$ of 50 ${\mu}g/mL$ through apoptosis, confirmed by DNA condensation and fragmentation. The cell population of cell cycle sub-G1 as indicative of apoptosis was also increased. In xenograft model in SD rats, F2 at dosage of 35 mg/kg weight was intravenously injected every two days. This reduced the tumor growth in magnetic resonance imaging images. The immunohistochemistry revealed that the anticancer activity might be mediated through inhibition of proliferation judged by Ki67 and apoptosis induced by activation of caspase-3 and -8. And the lowered expression of CD31 showed the reduction in blood vessel densities. The expression of matrix metalloproteinase-9 for invasion of cancer was also inhibited. The cell populations with cancer stem cell markers of CD133 and nestin were reduced. The results of this study suggested that F2 could be a new potential chemotherapeutic drug for GBM treatment by inhibiting the growth and invasion of cancer.

Anti-Cancer Effect of Ginsenoside F₂ against Glioblastoma Multiforme in Xenograft Model in SD Rats

Ji Yon Shin,Jung Min Lee,Heon Sub Shin,Sang Yong Park,Jung Eun Yang,Somi Kim Cho,Tae-Hoo Yi 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.1

The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside F₂ (F2) to treat GBM. In in vitro experiments with glioblastoma cells U373MG, F2 showed the cytotoxic effect with IC?? of 50 ㎍/mL through apoptosis, confi rmed by DNA condensation and fragmentation. The cell population of cell cycle sub-G1 as indicative of apoptosis was also increased. In xenograft model in SD rats, F2 at dosage of 35 ㎎/㎏ weight was intravenously injected every two days. This reduced the tumor growth in magnetic resonance imaging images. The immunohistochemistry revealed that the anticancer activity might be mediated through inhibition of proliferation judged by Ki67 and apoptosis induced by activation of caspase-3 and -8. And the lowered expression of CD31 showed the reduction in blood vessel densities. The expression of matrix metalloproteinase-9 for invasion of cancer was also inhibited. The cell populations with cancer stem cell markers of CD133 and nestin were reduced. The results of this study suggested that F2 could be a new potential chemotherapeutic drug for GBM treatment by inhibiting the growth and invasion of cancer.

탄산칼슘이 포함된 HDPE 필름의 광산화후 생분해

신부영,이훈복,이일희,신용섭 嶺南大學校 工業技術硏究所 1995 工業技術硏究所論文集 Vol.23 No.1

대량생산 체제에서 기계적 산화 공정을 응용하여 공기 분위기에서 광분해성 마스터 뱃치(M/B)를 제조하였다. 제조된 M/B가 40 중량 % 첨가된 광분해성 HDPE 얇은 필름을 인플레이션 법으로 제조하였다. 필름의 광분해성을 시험하기 위하여 필름을 옥외에 노출시켜 기계적 물성 및 필름의 화학적 변화를 FTIR을 사용하여 관찰했다. 생분해 실험은 인큐베이터 및 토양 매립 실험을 동시에 실시했다. 광분해성 필름을 옥외 태양광 노출을 각각 0, 14, 28, 42일간 수행한 후 미생물 배양 시험을 행했다. 미생물 배양 시험에서 필름의 표면에 미생물의 성장과 포자의 형성을 관찰할 수 있었고 옥외 노출 기간이 증가함에 따라 필름 표면에 미생물의 성장이 증대되는 것을 관찰했다. 토양에 매립된 필름에서도 약간의 화학적인 변화를 관찰할 수 있었다. Photodegradable commercial master batch(M/B) was prepared by using mechanical oxidation during melt mixing with high shear under air environment. High density polyethylene(HDPE) thin film containing 40 wt % photodegradable M/B was prepared by inflation film processing. The film was exposed to the sunlight to observe the photodegradability. The degradability of film was determined by the loss of tensile properties and degradation was characterized by FTIR spectroscopy. Biodegradation of exposed and unexposed film was investigated by microbiological test and soil burial test, respectively. Photodegradable film(P-1) was exposed for periods of 0, 14, 28, 42 days before being mixed with fungi. It was found that microorganisms(fungi) growth on the surface of film was augmented as sunlight exposed time increased. Soil buried film showed the chemical changes of matrix polymer.

저투입벼 재배에 관한 경영사례분석

신용인,박주섭 충남대학교 농업과학연구소 1996 농업과학연구 Vol.23 No.2

This study is aimed to provide data of low-input rice cultivation for agricultural policy, to reveal the problems of low-input cultivation through comparing the economic result of low-input cultivation with the common one, to search for solution or mitigation of the problems of low-input cultivation, and to forecast the future prospect of low-input rice cultivation. The following were the results obtained from the survey and analysis. The working hours per 10a inputted 45.4 hours which is 32% more than 34.5 hours of common cultivation. Yield per 10a was 355kg which was 101kg less than 456kg of common cultivation. But the farm received price per kg was 1,984.9 won which was 547.9 won more than 1,436.5 won of common cultivation. Gross receipts per 10a was 704,438 won which was higher than 655,044 won of common cultivation, and management cost was 230,820 won which slightly higher than 188,157 won of common cultivation. Consequently, the income of low-input rice cultivation was 473,617 won which somewhat exceed to 466,887 won of common cultivation.

산화구리에 의한 배기 가스 중의 아황산 가스 제거 : 반응속도에 관한 연구 A Kinetic Study

신창섭,노용우,이태희 연세대학교 산업기술연구소 1985 논문집 Vol.17 No.2

The reaction of cupric oxide powder with 2% sulfur dioxide was investigated over a temperature range of 300-500℃ and over a particle size range of 0.051-3㎜. The experimental data were correlated well by the shrinking unreacted core model and the optimal reaction temperature was found to be 450℃. With average particle diameter less than 1㎜ and superficial gas velocity higher than 8.8 ㎝/sec, the reaction of cupric oxide powder with sulfur dioxide was found to be controlled by the chemical reaction and the intrinsic rate constant was k=8987 exp(-8134/RT).

한국판 Edinburgh Postnatal Depression Scale의 임상적 적용

김용구,허지원,김계현,오강섭,신영철 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : The EPDS (Edinburgh Postnatal Depression Scale) is a lO-items self-report scale designed as a specific instmment to detect postnatal depression by Cox et al. (1987). This study was to determine the optimal cut-offpoint ofthe K-EPDS for postpartum depression in Korea. Methods : The 239 pregnant women assessed their own psychiatric features with the Korean version of the Edinburgh Postnatal Depression Scale (K.-EPDS), Beck Depression Inventory (BDI), Beck Anxiety Inventory (BAI), Rosenberg Self-Esteem Scale (RSES) and Marital Satisfaction Scale (MSS) at 6 months ofpregnancy, 1 week after delivery, and 6 weeks after delivery. Subjects above 9 points of K-EPDS at 6 week after delivery were interviewed with Structured Clinical Interview for DSM-IV Axis I Disorders (SCID-I) to confirm postpartum depression. Results : The prevalence of postpartum depression was 12.6% (30/239 pregnant women) in our study. The total scores of K-EPDS at 6 weeks after delivery were higher significantly than those of nonnal group. The score gap of K-EPDS between the depressed pregnant group and the normal pregnant group was increased after the delivery. However, there were no differences in the epidemiological characteristics and the BDI scores at 6 weeks after delivery between groups. Using the AUC (area under the curve), the optimal point to assess the postpartum depression was revealed as 6 weeks after delivery (AUC=85.8%) or 24 weeks of pregnancy (83.7%). The cut-offpoint of K-EPDS to detect postpartum depression among pregnant women was 9/10score of K-EPDS (AUC= 81.8%). Conclusion : In the K-EPDS, cut-off score of 9/10 was optimal to assess the postpartum depression, and K-EPDS at 6 weeks of delivery was more useful than any other point of time. K-EPDS administrated during pregnancy may be a useful tool to find the vulnerability on the postpartum depression.

연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

제주도 해안선주변 1개정점(용두암 근처)에서 부유성갑각류 현존량의 일주변화

고유봉,신희섭,정용진 제주대학교 해양연구소 1989 해양자원연구소연구보고 Vol.13 No.-

Planktonic crustacean samples and hydrographic data were obtained seasonally at intervals of two hours for one day at one station(near the Dragon Head Rock) around the coast line of northern part in Cheju Island from 1987 to 1988. Seasonal average individuals were low in February (307 indv./㎥) and October (282 indv./㎥), and highest in August (3.459 indv./㎥). Most of individuals were small animals retained on fine mesh(90μm mesh) net, composing over 91% of the total crustaceans. Range of seasonal average biomass was 3.0~20.5㎎/㎥. Generally, individuals and biomass of small planktonic crustaceans were higher during the daytime than those at night. whereas those on medium or large organisms were remarkably higher during the nighttime, Daily mean production, and ratio of daily production to biomass(P/B ratio) were highest in August and lowest in February, showing the larger the number of individuals and water temperature. the greater daily production and P/B ratio.

제주도 북촌연안 수산자원 유영생물의 군집구조와 먹이연쇄에 관한 연구 : II. 주요생물의 식성

고유봉,신희섭,정용진 제주대학교 해양연구소 1989 해양자원연구소연구보고 Vol.13 No.-

수용성 및 가식성 콩 단백질/�틴 블렌드 필름 제조 및 물성 연구

김성철,신용섭,정세환,유성구,박인식,김봉식,신부영 한국공업화학회 2003 응용화학 Vol.7 No.2

The manufacture of edible/biodegradable films or coatings can potentially add value to soy protein, Such films can find using as edible or nonedible food packaging materials. The effect of soy protein film-forming solutions on film formation, tensile properties of soy protein/pectin blend films were changed significantly by the amount of glycerol and the ratio of soy protein/pectin. Moreover mechanical properties of soy protein/pectin-montmorillonite clay have been studied. Tensile strength and modulus decreases as the relative humidity increased while with increasing relative humidity elongation at break increased.