急性骨髓性 白血症에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1

To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2×105 cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containg 20 or more cells, and cluster was defined as containing 3 - 19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10^(5) cells (20±19)and that of colonies was 5-24/2×10^(5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2×10^(5) cells(18±16) and that of colonies was 6-13/2×10^(5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases were 'non-forming', 2 cases were 'cluster forming'; and 3cases wer cluster and 'colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of 'cluster forming'; and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철산,강원권,김민범,김삼용,노홍규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1991 No.-

急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

To evaluate the in vitro granulocyte-macrophage colony formation in acute myfloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2 × 10 exp (5) cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defi ned as containg 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2 × 10 exp (5) cells (20±19)and that of colonies was 5-24/2 × 10 exp (5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2 × 10 exp (5) cells(18±16) and that of colonies was 6-13/2 × 10 exp (5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation: 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases Were `non-forming', 2 cases were `cluster forming'; and 3cases wer cluster and `colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of `cluster forming'; and none of 3 cases of `cluster and colony forming' achieved complete remission. So, there was significa nt difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

일개 농촌지역의 지역사회 영양정보에 관한 조사연구 : 경기도 남양주군 수동면을 중심으로 Su-Dong Myun, Nam Yang-Ju Gun, Kyung-Gi Do

계난이,권계숙,송미희,이연주 이화여자대학교 의과대학 1988 梨花醫學誌 Vol.- No.20

From September to November in 1987, the study was conducted a survey of Su-Dong Myun, Nam Yang-Ju Gun, Kyung-Gi Do, a comprehensive rural care demonstration area of Ewha Womans University. The purpose of this survey has been to examine into community nutrition informathrough preventive medicine training of sophomores of medical course. The result were as follows 1) Female respondent was 88.2% of all whose age was the forties and fifties at the highest rate of 22.6% respectively and whose educational level was uneducated at rate of 31.2%. 2) Agriculture(66.7%) was the common householder's occupation whose main income was from their own farm products(67.7%). 3) A family of five(24.7%) and two generations(e.g parent and children) (55.9%) was the most common family. 4) The housewife(98.9%) was the most common cook and 35.5% of all cook was not able have sufficient time to prepare for their meal. 5) On food purchase, main food was largely self provided(65.6%) and subsidiary food with self-provision and outside purchase(51.6%). 6) On the self sufficient food rice(77.4%) was most common as main food and vegetable(81.7%) as subdiary food. 7) On the frequency of purchasing food, main food was about 2-3 times a month, subsidiary food 1-3 times a week. On the market place, main food mainly self sufficient(59.1%) and subsidiary food was largely purchased from the peddlers and market on the move(35.5%) 8) On the means of transportation of purchased food, main food was conveyed by bus or another kinds of vehicles and subsidiary food(52.7%) was in the same way. 9) On the consumer's difficulty in purchasing food, meat and fish were rated 76.3% and vegetable 78.5%, 76.3% of all consumer had difficulty in purchasing meat and fish, 78.5% in purchasing grocery. 10) 94.6% was in possession of electric refrierator and 89.2% possessed their own wells. 11) The respondent had such fuels for cooking as propanegas(66.6%), wood(18.3%) briquet(10.8%). 12) the present state of nutrition was that the mean points was 2.7 in the group 1, 2.2 in the group 2, 3.9 points in the group 3, 2.8 points in the group 4, 3.1 points in group 5 and 2.7 points in the group 6. 13) Most families' favorite food was kimchi or kimchi soup and distasteful was meat including dog meat. 14) 48.4% of all respondents took no interest in taking in adequate nutrition owing to the lack of money and time to spare.

Enterococcus faecium에서 Vitek GPS Card의 Imipenem에 대한 위감수성

이혜경,박연준,궈희정,이은정,김병기,강창석 대한임상검사정도관리협회 2003 臨床檢査와 精度管理 Vol.25 No.2

대구, 경북지역 대학생의 식사행동 및 일본음식에 대한 인상 및 기호도 조사 연구

한재숙,이연정,최석현,최수근,권상용,최영희 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.1

본 연구는 대구, 경북지역의 대학생(570명)을 대상으로 식사내용, 식사관습, 식사예절, 일본음식에 대한 인상과 시식경험 및 기호도를 조사한 것으로 그 분석결과를 요약하면 다음과 같다. 1. 음식은 항상 일인분씩 담는다’의 경우는 전체응답자의 15.5%에 불과하였고, ‘음식을 큰 접시에 모아서 담는다’의 경우는 ‘가끔 한다’가 44.8%, ‘항상 한다’가 35.8%로 나타나 큰 접시에 모아서 담는 가정이 많았다. ‘저녁은 가끔 가족이 함께 모여서 먹는다’가 59.9%로 가장 많았고, ‘항상 가족이 모여 같은 음식을 먹는다’는 끼니는 아침이 42.3%, 저녁이 23.3%, 점심이 3%로 나타나 아침에 가족이 모여 같은 음식을 먹는 경우가 가장 많았다. 2. ‘식사시 가족의 자리가 정해져 있다’고 한 경우는 전체응답자의 53.5%였고, ‘가족이 모여 식사할 경우, 연장자가 수저를 들기 전에는 먹지 않는다’는 전체의 56.4%였으며, ‘가장에게는 음식의 양이나 수가 많다’는 30.9%였다. 3. 식사예절에 대해 가장 자주 주의를 받는 것은 ‘TV를 보지 않고 먹기’(13.4%), 남기지 않고 먹기(11.5%), ‘수저사용법’(8.0%)의 순으로 나타났다. 반면 ‘식기 부딪히는 소리’(76.9%), ‘입 다물고 먹기’(76.6%), ‘씹는 소리’(74.6%), ‘밥 먹는 모양’(71.4%), ‘수저 사용법’(69.7%)등은 전체응답자의 70% 이상이 ‘주의를 받지 않는다’고 하였다. 4. 음식 만들기에 대한 선호도는 5점 만점에 3.48점으로‘보통 이상’으로 나타났고 국가별 요리에 대한 기호도는 한국요리(4.39점)가 가장 높았고, 그 다음이 중국요리 (3.76점), 이태리요리(3.45점), 일본요리(3.32점) 순이었으며 프랑스요리(3.16점)가 가장 낮은 기호도를 보였다. 음식 만드는 빈도는 한달에 1∼2회 정도로 나타나 대학 생들이 직접 음식을 만드는 빈도는 매우 낮음을 알 수 있었다. 5. 일본 방문경험은 조사대상자의 93.8%가 없었으며, 그중 92.6%가 일본방문을 희망하였고 일본음식에 대한 이미지는 ‘가격이 비싸다’(4.15점), ‘장식이 아름답다’(4.05점), ‘색채가 예쁘다’(3.98점) 등은 높은 점수를 보인 반면에 ‘맵다’(2.21점), ‘기름기가 많다'(2.51점), ’깊은 맛이 있다‘(2.56점) 등은 낮은 점수를 보였다. 6. 일본음식 중 가장 높은 시식경험을 가진 것은 우동(95.3%)이었고 그 다음은 스시(93.1%), 덴뿌라(81.9%)순 이었고, 반면에 니쿠자가(6.4%), 오차즈께(9.3%), 오코 노미야끼(11.1%), 다코야끼(16.0%) 등은 매우 낮은 시식경험을 보였다. 7. 일본음식에 대한 기호도는 우동(3.98점), 스시(3.85점), 덴뿌라(3.69점), 소바(3.43점), 스키야끼(3.12점)는 대체로 높은 기호도를 나타낸 반면 낫또(2.68점), 오차즈께(2.76점), 오코노미야끼(2.87점), 미소시루(2.88점), 다코 야끼(2.88점) 등은 낮은 기호도를 보였다. 이상의 연구결과로 변모해 가는 우리전통 식사예절의 동향 파악이 가능하였고 가족간의 화목과 건강한 사회를 이루기 위한 가족 단란의 식사지침이 요구되었다. 한편 일본음식중에는 우동과 스시가 한국대학생들에게 가장 인기가 높은 음식임을 알 수 있었고 대부분의 일본요리에 대해 장식이 예쁘고 색채가 아름답지만 값이 비싸다는 이미지를 가지고 있어서 가격을 좀 더 낮춘다면 그 이용이 더욱 늘어날 것으로 기대되었다. 또한 낫또, 오차즈께, 오코노미야끼 등 이용도가 낮은 음식에 대해서는 한국인의 입맛에 맞는 요리법을 가미한다면 대중화가 가능하리라고 여겨진다. This study was conducted to investigate the dietary behavior and image and preference of Japanese foods. The Subjects were consisted of 570 university students(243 males and 327 females) in Daegu and Kyungbuk area, Korea. The students responses to the 10 questions about image of Japanese foods were also measured on 5 point Likert scale. Data were presented by using frequency, percentage, chi-square test and T-test. The results of this study were as follows: (1) On the eating habits, 'the whole family has breakfast together with same foods everyday' scored high as 42.3% and 'foods put in a big platter by gathering everyday' as 35.8%. (2) About the eating customs, 53.5% of the subjects responded that the seat was fixed at meal time, 56.4% didn't start to eat before the patriarch started a meal and 30.9% responded that the head of a family had more foods in number and quantity. (3) On the table manners, 13.4% of the subjects were scolded about 'watching TV on eating', 11.5% about 'making left-over foods', 8.0% about 'misuse of spoon and chopsticks'. (4) The preferred ethnic foods by University students was in other of Korean, Chinese, Italian, Japanese and French foods. (5) Among subjects, 93.8% had no experience of visiting Japan and 92.6% wanted to visit Japan. Images on the Japanese foods were 'the price is too expensive' (mean 4.15) and 'the decoration is wonderful'(mean 4.05). But the subjects did not think Japanese foods as 'hot'(mean 2.21) and 'greasy'(mean 2.51). (6) The favorite Japanese food of subjects was Udon(mean 3.98), Sushi(mean 3.85) and Tempura(mean 3.69). So Udon turned out to be the most popular Japanese foods by university students in Daegu and Kyungbuk area, Korea. But they did not prefer Natto(mean 2.68), Ochazuke(mean 2.76), Okonomiyaki(mean 2.87) and Misosirn and did not eat. From the above results, Korean university students preferred Udon to Natto among Japanese traditional foods, and they estimated Japanese foods as 'too expensive'. Therefore, lowering the price and developing the cooking method for Korean taste were needed to increase the intake of Japanese traditional foods by Korean university students and.

양파(Allium cepa L.) 추출물이 Tyrosinase 유전자 발현에 미치는 효과

조남철,윤연희,이혜진,손현정,김양경,최근희,나명석,조영권,이황희,진종언 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.3

Melanin 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase promoter를 지닌 Bl6 mouse melanoma cell해 앙파 methanol 추출물을 처리한 바 양파 methanol 추풀물은 10.0㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml의 농도에서 대조군에 비해서 약 15%, 23% 57%의 억제효과를 나타냈으며 세포생존율은 1.0 ㎍/ml, 10.0 ㎍/ml, I00.0 ㎍/ml, 1.0㎎/ml의 농도에서 약 126 %, 92%. 85%, 64%로서 세포독성이 낮게 나타났다. Ethyl acetate, butyl alcohol, 그리고 물 용매 분획물은 tyrosinase 유전자의 발현물 억제하는 효과가 없었지만 methylene chloride 용해 분획물은 10.0 ㎍/ml과 100.0 ㎍/ml의 농도에서 약 37%와 35%의 발현율을 나타냄으로서 대조군에 비해 크게 억제하였다. Onion(Allium cepa L,) extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10.0 ㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml of the extract repressed expression of tyrosinase gene about 15%, 23%, and 57%, respectively, compared with control. In the MTT assay, the same extract exhibited low cytotoxicity at 1.0 ㎍/ml, 10.0 ㎍/ml, 100.0 ㎍/ml, and 1.0 ㎎/ml, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of methylene chloride repressed highly at 10.0 ㎍/ml and 100.0 ㎍/ml.

미용사의 요통 경험에 영향을 미치는 요인

백승룡,남철현,배성욱,김성우,권현숙,이정옥,전연숙 慶山大學校 保健福祉硏究所 2002 保健福祉硏究 Vol.7 No.-

The purpose of this study is to analyze and grasp the actual lumbaginous condition and relevant factors of 1,182 beauty artists of beauty shops in 5 metropolitan cities, small-to-medium cities, and fishing or agrarian villages for 3 months from June 20 to September 25, 2000 through questionnaires. 1) They showed 61.0 percents in her twenties, 66.4 percents of unmarried women from all the female objects, 70 percents in high school graduates as educational background, 52.4 percents in metropolitan city as service place, 25.4 percents in smoking rate, 71.6 percents in drinking rate, 48.1 percents in general bed as condition of bedroom, 17.9 percents in exercising rate, 53.6 percents in 'little eating', 39.6 percents in two or three centimeters of heel, and 89.5 percents in hair as beauty-related professional field. 2) The rate of lumgaginous experience of beauty artists was 83.5 percents. They showed higher rate of 91 percents in the thirties and forties, 92 percents of the married, 93.9 percents in junior college graduates, 97.2 percents in higher economic classes, 88.7 percents in smokers, 85 percents in general bed, 89.3 percents in sleeping on one's face, 85 percents in swimming, 92 percents in stretch, 86.7 percents in 56 to 60 kilograms of weight, 89.7 percents in group O blood, 90.5 percents in experienced women of abortion, 97 percents in heel over 8 centimeters, 58.3 percents in 'feel much fatigue', 93.6 percents in inconvenient chair of beauty shop, and 89.4 percents in a lack of recess than other group. 3) The more women who have been experienced in lumbago, the higher their social treatment(p<0.01), income(p<0.001), working time(p<0.05), job potentiality(p<0.05), inner affinity(p<0.05), and job satisfaction of environment(p<0.001) were. Women who suffered much stress related to job environment(p<0.001), client(p<0.01), and their duty(p<0.01) showed high rate of lumbaginous experience. 4) In degree of lumbago by characteristic, there was the significant difference in age over 50-years-old, unmarried women, college graduates, service of small-medium cities, higher economic classes, smokers or drinkers, hot-floored room, sleeping with ransack, stretch or swimming, group A blood, fat body, experience of abortion, 6 to 7 centimeters of heel, convenient chair of beauty shop, insufficient recess, lumbago continued for one to the three months in comparison of other group. 5) There were variables of posture such as to place materials under one foot(p<0.001), to lay arm on armrest and desk(p<0.001), to pull materials near body while lifting them (p<0.05), to sometimes use anodyne(p<0.01), not to lift also light materials(p<0.001), to be inconvenient in sleeping(p<0.001), to be absent at job for two or three days(p<0.01). The degree of depression(p<0.001), had the significant effect on the degree of lumbago(32.7%). The results are as follows : Beauty artists have faced upon dangerous job environment to cause lumbago. The more the industrial society will be developed, the more their burden of service will be increased. In addition, the occupational stress and posture of beauty artists who showed high job satisfaction and high economical level became major factors which have had an effect on their lumbago. In conclusion, it is desirable for beauty-related organizations and professionals to positively develop program of health education with much concern as well as to lessen stress of beauty artists through education of job posture and rightful life in order to prevent lumbago of those beaut.

사망 진단서(시체 검안서) 작성의 문제점

김규석,임용수,이중의,서길준,윤여규,어은경,염석란,정연권,이윤성 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: This study was conducted to analyze the current problems in completing death certificates and to identify the correct method for completing death certificates Methods: We reviewed 262 death certificates in three hospitals from March 1 to April 30, 2000, and 119 death certificates in one hospital from March 1 to 31, 2000. We identified major and minor errors and analyzed and compared them retrospectively. Results: A total of 381 death certificates were reviewed: 59 in Seoul National University Hospital,101 in Ewha Woman's University Hospital, and 102 in Gachon Medical College Hospital, which has no education program for completing death certificates in postgraduate training, and 119 in Samsung Medical Center which has an education program for completing death certificates. 358 certificates(94.0%) had at least one error. There were only 23 death certificates(6.0%) without an error. In 182 cases(47.8%), there was one major error. In 321 death certificates(84.3%), there were more than two errors. A comparison of Samsung Medical Center with the other hospitals showed that the number of total errors was statistically different(p=0.001). Conclusion: There were few death certificates without an error in this study. In a hospital which has postgraduate training in completing death certificates, there are fewer errors than in other hospitals which have no training course. Emergency physicians actually certify many deaths, so they must know the correct method of completing death certificates for statistics on morbidity and mortality.

Injectable in situ-forming hydrogel for cartilage tissue engineering

Kwon, Jin Seon,Yoon, So Mi,Kwon, Doo Yeon,Kim, Da Yeon,Tai, Guo Zhe,Jin, Ling Mei,Song, Boram,Lee, Bong,Kim, Jae Ho,Han, Dong Keun,Min, Byoung Hyun,Kim, Moon Suk The Royal Society of Chemistry 2013 Journal of materials chemistry. B, Materials for b Vol.1 No.26