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Bacillus 속 분리주가 생산하는 박테리오신의 특성 조사
함승희(Seung-Hee Ham),최낙식(Nack-Shick Choi),문자영(Ja-Young Moon),백선화(Sun-Hwa Baek),이송민(Song-Min Lee),강대욱(Dae-Ook Kang) 한국생명과학회 2017 생명과학회지 Vol.27 No.2
토하젓에서 분리한 박테리오신 생산 균주 중 상대적으로 넓은 항균스펙트럼을 나타내는 1균주를 선발하였고 16S rRNA 유전자 염기서열 분석 결과 B. subtilis E9-1와 거의 일치하는 것으로 동정되었다. B. subtilis E9-1가 생산하는 박테리오신의 물리화학적 특성을 조사하였고, 박테리오신을 정제하였다. 이 박테리오신은 B. cereus KCCM 11204, M. luteus IAM 1056, L. monocytogenes KCCM 40307, E. faecium KCCM 12118 및 S. aureus subsp. aureus KCCM 40050 등에 대해서 항균활성을 나타내었다. pH 2.0~8.0 범위에서는 안정하였으나 8.0 이상에서는 항균활성이 감소하였다. Isopropanol, ethanol 및 methanol 등의 유기용매에서 100%까지, acetone과 acetonitrile에서는 80%까지 항균활성을 유지하였다. 내열성의 경우 40~100℃에서 60분까지는 안정하게 항균활성을 보였다. 박테리오신 농도를 증가시키면서 B. cereus, L. monocytogenes, E. faecium 및 S. aureus subsp. aureus 등 시험균 4주의 감수성을 조사한 결과 농도 의존적으로 시험균의 생육이 감소하였고 이중 L. monocytogenes 의 감수성이 가장 높게 나타났다. 박테리오신의 작용양상을 알아본 결과 B. cereus와 L. monocytogenes 배양액에 박테리오신 용액을 첨가한 후 흡광도와 CFU값이 감소하여 bactericidal임을 확인하였다. 식품에 적용 가능성을 알아보기 위해 실험한 결과 3일째부터 박테리오신을 처리하지 않은 대조구에 비해 실험구에서 생균수(CFU/ml)가 감소하였다. 아세톤을 이용한 배양상등액의 농축, superdex peptide HR 10/300 column 이용한 겔여과크로마토그래피, 역상 HPLC로써 박테리오신을 정제하였다. 역상 HPLC를 통해서 정제한 박테리오신의 분자량을 tricine SDS- PAGE로 분석한 결과 약 4 kDa이었고 질량분석법으로 측정한 정확한 분자량은 3347.6 Da로 나타났다. As an effort to find a potential biopreservative, we isolated bacterial strains producing bacteriocin from fermented foods. A strain was finally selected and characteristics of the bacteriocin were investigated. The selected strain was identified as Bacillus subtilis E9-1 based on the 16S rRNA gene analysis. The culture supernatant of B. subtilis E9-1 showed antimicrobial activity against Gram-positive bacteria. Subtilisin A, α-chymotrypsin, trypsin and proteinase K inactivated the antimicrobial activity, which means its proteinaceous nature, a bacteriocin. The bacteriocin activity was fully retained at the pH range from 2.0 to 8.0 and stable at up to 100℃ for 60 min. Solvents such as ethanol, isopropanol and methanol had no effect on the antimicrobial activity at the concentration of 100% but acetone and acetonitrile reduced the activity at up to 100% concentration. Cell growth of four indicator strains was dramatically decreased in dose-dependent manner. Listeria monocytogenes was the most sensitive, but Enterococcus faecium was the most resistant. Bacillus cereus and Staphylococcus aureus showed the medium sensitivity. The bacteriocin showed its antimicrobial activity against B. cereus and L. monocytogenes via bactericidal action. The number of viable cells of L. monocytogenes started to reduce after addition of bacteriocin to the minced beef. The bacteriocin was purified through acetone concentration, gel filtration chromatography and RP-HPLC. The whole purification step led to a 6.82 fold increase in the specific activity and 6% yield of bacteriocin activity. The molecular weight of the purified bacteriocin was determined to be 3.3 kDa by MALDI-TOF/TOF mass spectrometry.
혐오자극과 모델링 및 언어적 지시가 발달지체 아동의 상동행동에 미치는 효과 : 사례연구
한현민,함소희,김윤선,지지영,홍희영 대진대학교 교육연구소 2000 교육연구 Vol.2 No.1
The experiment was undertaken to determine the combined effects of aversive, modeling, and verbal instruction on the self-stimulatory stereotyped benavior exhibited by a three-year two-month old with developmental delay, The behavioral interventions were consisted of lemon juice(aversive), behavioral rehearsal(moleling), verbal instruction(prompting), and defferential reinforcement of incompatible behavior (DRI), and continued over 73 sessions. The data gathered across multitreatments design (A-BCD-A-BD-DE) were visually analyzed. The results revealed that all the treatments reduced the occurrences of stereotyped behavior, the significant differences among any interventions were not found, and more importantly, the behavior effects were maintained.
한국인 주요우울장애 환자에서 혈중 ACTH, Cortisol 농도와 해밀턴 우울 평가 척도의 신체증상 항목과의 상관관계
여혜빈,김린,함병주,심세훈,권영준,정희연,정한용,한상우,이민수,이화영 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2
Objectives:Hypothalamic-Pituitary-Adrenocortical(HPA) system dysregulation plays an important role in the pathophysiology of major depressive disorder. Medically unexplained somatic symptoms comprised the predominant complaints of korean patients with major depressive disorder. It might be related to dysregulation of HPA system and somatic symptoms. The aim of this study was to investigate the association between Hamilton Depression Rating Scale severity and the Plasma Adrenocorticotropic Hormone(ACTH) & Serum Cortisol level in Korean patients with major depressive disorder. Methods:Our study design was prospective. A total 111 hospitalized patients with major depressive disorder (DSM-IV) at Anam Hospital, Korea University Medical Center between Jan 2009 and May 2011 were selected. Hamilton Depression Rating Scale was measured by psychiatrist. Plasma ACTH, Serum Cortisol levels were analyzed at the time of admission. Results:There are significant correlations(p<0.05) between Hamilton Depression Rating Scale of somatic component(HAM-D 11-15 item) and Plasma ACTH and Serum Cortisol level. However, other HAM-D sub items were not significantly correlated with Plasma ACTH and Serum Cortisol level except somatic component. Conclusion:Our Study results suggest that hyperactivity of HPA axis might be correlation with somatic symptoms in korean patients with major depressive disorder.
COVID-19 상황의 학교급식에서 위기대응식에 대한 급식관리자의 인식 조사
서민국 ( Min-guk Seo ),이민준 ( Min-june Lee ),민성희 ( Sung-hee Min ),함선옥 ( Sunny Ham ) 대한영양사협회 2022 대한영양사협회 학술지 Vol.28 No.1
This study aimed to evaluate the perception of nutrition teachers about emergency meals in all schools affiliated with the Seoul Office of Education. The purpose of this study was to suggest the need for emergency meals and provide directions for future development, thereby assisting meal service sites. An online survey was conducted from May 26 to 31, 2021, analyzing 130 collected samples. The summary of the results is as follows: First, the nutrition teachers recognized the need for school meal services for the prevention of the spread of infectious diseases and considered the variance among school meal consumers. Also, they generally thought of emergency meals due to the need for social distancing and the decrease in the distribution of food. Secondly, in terms of the differences in the perception about emergency meals, nutrition teachers (45.4%) who have provided emergency meals (t=2.584, P<0.05) were more aware of the need for emergency meals than nutrition teachers (54.6%) who had not provided emergency meals. Nutrition teachers conceived emergency meals to minimize the contact between people (45.6%) and to rectify the imbalance in nutrition (37.5). Next, emergency meal attributes were observed to be ranked in the order of convenience (3.49), safety (3.15), and satisfaction (2.88). Fourthly, although there were no meaningful differences in emergency meal attributes, there were statistically significant differences in the safety of the cooking process, menu familiarity, the low unit price compared to regular meals and the excellent satisfaction with nutrition provided (t=2.603, P<0.05), (t=2.039, P<0.05), (t=2.154, P<0.05), (t=2.477, P<0.05) respectively.
The Effect of Sex on the Azoxymethane/Dextran Sulfate Sodium-treated Mice Model of Colon Cancer
Sun Min Lee,Nayoung Kim,Hee Jin Son,Ji Hyun Park,Ryoung Hee Nam,Min Hee Ham,Daeun Choi,Sung Hwa Sohn,Eun Shin,Young-Jae Hwang,Jihee Sung,Dong Ho Lee,Ha-Na Lee 대한암예방학회 2016 Journal of cancer prevention Vol.21 No.4
Background: The colitis-associated cancer exhibits different characteristics according to sex in the initiation and progression of the tumors. The aim of this study was to investigate the sex-associated difference in the azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colitis-associated cancer model. Methods: The AOM/DSS ICR mouse model was established to compare male with female, and then the severity of colitis-associated carcinogenesis was examined macroscopically and histologically regarding the number, size, and location of tumors. Subsequently, levels of colonic mucosal cytokine, interleukin (IL)-1 and myeloperoxidase (MPO) were assessed. Results: At the 16th week, the tumor multiplicity and the pro-inflammatory factors differed according to sex. The total tumor number was significantly higher in male (P = 0.020) and the number of large tumors (diameter > 2 mm) was higher in male (P = 0.026). In male, the tumors located more in distal colon (P = 0.001). MPO was significantly higher in AOM/DSS-treated male mice compared to the control group (P = 0.003), whereas the corresponding female group showed no significant change (P = 0.086). Colonic IL-1β level significantly increased in AOM/DSS groups compared to control groups both in male and female (male, P = 0.014; female, P = 0.005). It was higher in male group; however, there was no statistical significance (P = 0.226). Conclusions: In AOM/DSS murine model, colitis-associated colon tumorigenesis are induced more severely in male mice than female probably by way of inflammatory mediators such as IL-1 and MPO. The sex-related differences at the animal model of colon cancer suggest the importance of approach to disease with sex-specific medicine in human.
Alkyl/Vinyl-표면개질된 Nanosilicas의 LSR-나노콤포지트 HVDC 전기적 및 기계적 특성
함성호(Seong Ho Ham),송창훈(Chang Hun Song),최승민(Seung Min Choi),장희수(Hee Soo Jang),유재우(Jae Woo Yoo),윤영빈(Young Been Yoon),이병훈(Byung Hun Lee),김찬성(Chan Seong Kim),조승민(Seung Min Jo),이지희(Ji Hee Lee),박재준,김영선(Young 대한전기학회 2020 대한전기학회 학술대회 논문집 Vol.2020 No.7
나노 실리카의 친수성 표면은 다양한 비율의 알킬실란 및 비닐 실란 커플 링 제로 변형시킴으로써 소수성 특성으로 변화된다. 실리콘 고무 나노 복합체에서 고전압 직류(HVDC) 항복 전압 및 인장 특성에 대한 개질 된 나노 실리카의 효과를 중전기 장비에 적용하기 위해 연구 하였다. 표면 개질은 푸리에-변형 적외선 분광법 (FT-IR) 분석에 의해 확인되고 알킬 / 비닐 커플 링제의 중량은 열 중량 분석 (TGA)에 의해 측정된다. 실리콘 고무 나노 복합물은 액체 실리콘 고무(LSR)와 개질 된 나노 실리카를 혼합함으로써 제조된다. LSR대 나노 실리카의 혼합비는 20 중량 %로 고정된다. LSR 매트릭스에서 나노 실리카 입자의 균일 한 분산을 관찰하기 위해, 투과 전자 현미경 (TEM)이 사용되고 표면-개질 된 나노 실리카는 20 내지 100 nm 크기의 나노 클러스터 형태로 잘 분산되어있는 것으로 밝혀졌다. 알킬 실란 : 비닐 실란의 표면 개질 비가 50 일 때 전기적 특성 (± HVDC 파괴 강도 및 유전 특성)및 기계적 특성 (인장 강도 및 파단 신율)이 추정되며 ± HVDC 파괴 강도 및 인장 강도가 최대 : 50 중량 %.