섬진강 수계의 부착조류에 대한 생태학적 연구

최민규,김백호,최환석,이경보,이덕배,정연태 원광대학교 환경과학연구소 1997 環境科學硏究誌 Vol.6 No.1

신경성 염증질환에서 지질 신호전달물질인 lactosylceramide의 역할

이윤선, 유재명, 최경미, 이용문, 홍진태, 윤여표, 유환수 충북대학교 약품자원개발연구소 2007 약학논문집 Vol.22 No.-

-본문참고-

麥酒麥의 (1-3,1-4)-β-glucanase 遺傳子의 構造와 發現 樣狀

유남희,윤성중,최경구,김제환,박충웅 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

禾穀類 種子 發芽時 호분층과 ??組織의 細胞壁에 多量 含有되어 있는 (1-3, 1-4)-β-glucan을 分解하여 種子의 發芽에 必要한 加水分解酵素의 分泌 및 擴散 浸透를 돕는다. 本 硏究에서는 國內 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子의 構造 및 發現 에 대한 基礎 情報를 얻기 위하여 生化學的 및 分子生物學的 方法을 利用하여 發芽種子 및 어린 잎에서의 酵素活性과 遺傳子의 發芽樣狀 그리고 遺傳子의 構造 等을 調査하였다. 麥酒麥 發芽種子中 (1-3, 1-4)-β-glucanase 活性은 發芽 7日째에 제일 높았다. (1-3, 1-4)-β-glucanase의 活性測定에 使用한 (1-3, 1-4)-β-glucan이 (1-4)-β-glucanase에 의해 分解될 可能性이 있으므로 (1-4)-β-glucanase의 活性도 測定하였는데 (1-4)-β-glucanase의 活性이 매우 낮게 나타나서(1-4)-β-glucanase가 (1-3, 1-4)-β-glucanase을 分解하여 (1-3, 1-4)-β-glucanse의 活性에 影響을 미쳤을 可能性이 매우 낮았다. 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子 構造를 PCR을 利用하여 調査하였던바 國內 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子 構造는 外國의 다른 品種들이나 귀리의 遺傳子 構造와 대단히 類似하였다. 酵素活性 測定과 귀리의 (1-3, 1-4)-β-glucanase cDNA clone을 ?識子로 利用한 보리의 mRNA 分析에 의해서 發芽傳子 및 어린 잎에서 (1-3, 1-4)-β-glucanase가 種子發芽 以外에도 生育中인 組織細胞의 伸張과 發育 및 β-glucan 再利用에 泌要한 β-glucan 代謝에 關與하고 있음을 示唆하는 結果라 생각된다. (1-3, 1-4)-β-glucans hydrolysis of cell wall (1-3, 1-4)-β-glucanas accompanies degradation of endosperm cell walls during cereal seed germination. To investigate (1-3, 1-4)-β-glucanase expression pattern in Korean malting barley, (1-3, 1-4)-β-glucanase activity, gene structure and mRNA expression patterns werre examined. (1-3, 1-4)-β-glucanase activity increased as germination proceeded and the highest enzyme activity was detected in seeds germinated for 7 days. Because (1-4)-β-glucanase have a potential to degrade (1-3, 1-4)-β-glucans, (1-4)-β-glucanase activity was also determined. (1-4)-β-glucanase activity, however, was very low minimizing possibility of (1-4)-β-glucanase action on (1-3, 1-4)-β-glucan substrate. PCR technique was employed to investigate (1-3, 1-4)-β-glucanase gene structure. The amplification product of the expected size from the targeted sites of Dusan #29 indicated that (1-3, 1-4)-β-glucanase systems were conserved in different barley varieties and even in oats. (1-3, 1-4)-β-glucanase activity and mRNAs were detected in young leaves as well as in germinating seeds. This may suggest that (1-3, 1-4)-β-glucanase are also involved in β-glucan metabolism required for cell elongation and β-glucan reutilization during leaf growth and developmetn.

항 Vancomycin 내성균성 항생물질을 생산하는 Streptomyces sp.AMLK-335의 배양조건

이기형,임대석,최경희,이경호,김창한,김정환 건국대학교 동물자원연구센터 2000 動物資源硏究誌 Vol.21 No.-

본 연구는 새로운 항 vancomycin 내성균성 항생물질을 개발하기 위하여 실시되었으며, 전국 각지의 토양 시료로부터 분리한 방선균 중 vancomycin 내성균주인 K-98 Enterococcus faecium 642, K-98 Enterococcus faecium 637 균주에 대하여 항생물질을 생산하는 분리균주 AMLK-335를 선정하여 이 균주의 항생물질 생산에 대한 최적 발효 조건을 검토한 결과, 탄소원 첨가로는 glycerol(0.5%), 질소원 첨가로는 yeast extract(0.5%)가 가장 효과적으로 항생물질 생산에 이용되었고, 비타민을 첨가에 따른 항생물질 생산성의 차이는 크게 나타나지 않았으나, calcium pantothenate 첨가시 항균활성이 비교적 높아졌으며, 금속이온의 첨가는 K+, Pb+, Ba²+, NA²+ , Ca²+를 첨가하였을 때는 항생물질의 생산성이 비교적 높아졌으며, 그중 Ca²+ 첨가구가 가장 생산성이 높았으며, Fe²+ , Hg+의 금속이온을 첨가하였을 때는 항생물질 생산성이 감소하였으며, CaCO₃첨가는 비첨가구보다 높은 항생물질 생산성을 보였으나, 첨가량에 따른 영향은 대체적으로 없었다. pH의 변화에 따른 생산성은 전반적으로 pH가 항생 물질 생산에 영향을 미치지 못하였으나, pH 4.5∼6.0 부근에서는 비교적 높은 항생물질 생산성을보였으며, 통기량에 따른 항생물질 생산성은 통기량이 많을수록 항생물질 생산이 좋았다. 한편 배양기간에 따라 항생물질 생산성을 조사한 결과, 5일째에 가장 높은 항생물질 생산성을 나타내었으며, pH의 변화는 배양 초기에 하강하였다가 점차 상승하는 경향을 보였다. This study was carried out for examining antibiotic productivity of the strain Streptomyces sp. AMLK-335 producing anti-VRE antibiotic. According to different condition, the strain was cultured for 4 days at 27℃ and determined cell growth, pH value and antibiotic productivity using disk diffusion method. In the experiment, the strain Streptomyces sp. AMLK-335 was examined various culture conditions which was added 8 carbon sources, 12 nitrogen sources, 8 vitamins and 11 metal ions in each time and controlled concentration of CaCO₃, initial pH and aeration. From the studies of conditions for antibiotic production, antibiotic productivity was the best to use 0.5% glycerol as carbon source and 0.5% yeast extract as nitrogen source. When several vitamins were added to medium, there was little difference in antibiotic productivity. But the addition of calcium pantothenate slightly increased antimicrobial activity. The addition of metal ion, such as K+, Pb+, Ba²+, NA²+ and Ca²+ slightly increased antibiotic productivity except Fe²+ and Hg+. The addition of CaCo₃showed higher than non-addition experiment in antibiotic production, but there wasn't generally influenced at addition concentration. According to pH change, antibiotic productivity was increased at pH 4.5∼6.0, and when there was a lot of aeration, antibiotic productivity was increased.

접촉각 측정에 의한 사이즈 분석

이찬용,김철환,최경민,박종열 경상대학교 농업생명과학연구원 2001 농업생명과학연구 Vol.35 No.-

접촉각(Contact Angle)이란 액체가 고체표면 위에서 열역학적으로 평형을 이룰 때 형성하는 각을 의미하며, 고체와 액체 사이에서 이루는 접촉각의 측정은 접착, 표면처리, 폴리머 표면 분석과 같은 많은 분야에서 잘 알려진 기술이다. 이러한 접촉각은 종이의 내수성에 대한 정밀한 예측을 가능하게 하는데 본 연구를 통하여 개발된 접촉각 자동 측정 시스템(AMS-2001)은 종이에 부여된 내수성을 종이 시료 상에 형성된 접촉각 측정을 통하여 정량화 할 수 있었다. 본 연구에서 개발한 AMS-2001을 이용하면 사이징 처리된 종이의 접촉각, 발수각, 액적의 폭, 지름, 표면장력 등을 측정할 수 있었고, 액적이 변화되는 모양을 디지털 화상을 통하여 추적할 수 있었으며 또한 기존의 Stockigt 실험 방법과 거의 유사한 재현성 및 신뢰성을 나타낼 수 있었다. The contact angle formed by a droplet of water used to estimate a repellency property of paper. The conventional systems measuring a contact angle are based on a manual measurement of the angle by a 3-point determination such as a far-left or far-right point, and a top point of a water droplet on a sample sheet. Thus the manual method to analyze a water repellency property can be greatly affected by an operator's bias. The new method to measure a sizing degree by a contact angle was developed using an automatic determination of 3-end point coordinates of the water droplet, which could diminish the operator's bias during measurement. The system could also quantify width and height of a water droplet, a surface tension of a solid specimen, and a time elapsed during complete absorption of water into a sheet. Finally, the values measured by the system were quite reproducible and reliable.

석청(Mad-honey) 복용 후 유발된 부정맥 1예

김용철,김남호,김승환,최준호,박은미,이상재,이은미,유남진,윤경호,오석규,정진원 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

석청에 포함된 grayanotoxin에 의해 독성 작용이 나올 수 있으며, 이러한 독성 작용은 일반적으로 24시간 이내 저절로 회복된다. 최근에 본 저자들은 석청 복용 후 발생한 심실빈맥을 경험하였기에 보고하는 바이다. 49세 남자가 호흡곤란으로 내원하였으며, 내원당시 분당 40회 정도의 동성 서맥이 관찰되었고, 수액을 투여하던 중 심실 빈맥이 발생하였다. 항부정맥제를 투여 후 정상 동율동으로 전환되었으며, 특별한 이상 없이 4일 후 퇴원하였다. Mad-honey intoxication caused by the consumption of honey producted from the nectar of rhododendrons. The grayanotoxins cause the intoxication. The toxic effects of mad-honey poisoning are rarely fatal and generally last for no more than 24 hours. We experienced one case, a 49 years-old man who presented with dyspnea after ingestion of mad-honey. He showed marked sinus bradycardia with < 40 beats per minute on admission. The cardiac rhythm was changed to ventricular tachycardia immediately. These features resolved completely in 24 hours with continuous infusion of amiodarone(600 mg per day) and fluids. We report the case of intoxication of mad-honey as a presentation of fatal cardiac arrhythmia.

Inverse Relationship between Adipocyte Differentiation and Ceramide Level in 3T3-L1 Cells

Choi, Kyeong-Mi,Lee, Youn-Sun,Choi, Mi-Hwa,Sin, Dong-Mi,Lee, Seunghyun,Ji, So-Young,Lee, Mi Kyeong,Lee, Yong-Moon,Yun, Yeo-Pyo,Hong, Jin-Tae,Yoo, Hwan-Soo Pharmaceutical Society of Japan 2011 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.34 No.6

<P>Adipocyte differentiation has been a target in anti-obesity strategies and is known to be closely related to lipid metabolism. Ceramide, a major sphingolipid metabolite, has been implicated in differentiation. In this study, we investigated whether ceramide biosynthesis is related to adipogenesis in 3T3-L1 cells. Preadipocytes can be differentiated synchronously by a mixture of adipogenic inducers including 3-isobutyl-1-methylxanthine, dexamethasone and insulin. The number of lipid droplets and the triglyceride content, which are differentiation biomarkers, gradually increased during adipogenesis. Interestingly, ceramide and sphingosine contents in the differentiated cells were decreased compared to those in preadipocytes. When the preadipocytes were treated with an 3-isobutyl-1-methylxanthine- or dexamethasone- or insulin-deficient mixture of inducers, the cellular ceramide levels were significantly increased compared with those in cells treated with the complete set of inducers. When preadipocytes were treated with 0, 0.1 or 1 μg/ml insulin along with 3-isobutyl-1-methylxanthine and dexamethasone, the ceramide levels were decreased and the triglyceride content was increased in a concentration-dependent manner. When the cells were treated with epigallocatechin gallate, an adipocyte differentiation inhibitor, during adipogenesis, the ceramide levels of adipocytes were increased and the fat content was decreased. In conclusion, our findings demonstrate that cellular ceramide levels are inversely correlated with adipocyte differentiation.</P>

한국제주도및남해연안에서식하는잘피거머리말의집단유전학구조a

Jae Hwan Kim,Ji Hyoun Kang,Sun Kyeong Choi,Sangil Kim,Ji Eun Jang,Hyuk Je Lee,Sang Rul Park 한국환경생태학회 2016 한국환경생태학회 학술대회지 Vol.2016 No.1

Seagrasses, sea flowering plants, comprise approximately 60 species globally and are often called ‘ecosystem engineers’ because they create their own habitats by modifying the surrounding environments, which provide coastal zones with a number of crucial ecosystem services. Zostera marina (the common name ‘eelgrass’) is one of the seagrass beds-forming species distributed widely in northern hemisphere including the Korean coast, which plays a pivotal role in ecosystem as a primary producer and a nursery habitat or refuge for other marine organisms. However, due to global climate change and anthropogenic activities such as reclamation and dredging, there has recently been a drastic decline in population sizes of Z. marina in Korea. In order to develop effective conservation and restoration management programs of Z. marina populations, it would be helpful to consider all biological aspects of this species such as genetic characteristics as well as ecological and physiological features. This study first provides information on genetic diversity and genetic structure of Jeju Island and Namhae populations of Z. marina, which will contribute to the establishment of appropriate conservation and restoration management plans for future persistence of this species. Using six microsatellite markers, we investigated the level of genetic diversity and genetic structure among 10 geographic populations of Z. marina inhabiting Jeju Island (Hamdeok, Tokki-seom, Sungsan, Woljeong, Ojo) and Namhae (Gamak bay, Jindong bay, Nampo, Anggang bay, Geoje) on the southern coast of Korea. The level of genetic diversity within Jeju populations (mean allelic richness [AR]: 1.57 ~ 3.09) was found to be significantly lower than Namhae populations (AR: 3.09 ~ 4.29) (Mann-Whitney U-test, P < 0.05). These findings suggest that effective population sizes (Ne) of Jeju populations are generally smaller than those of Namehae populations. Within Jeju Island, Hamdeok population had the smallest population size (coverage: 138 m2) and the lowest genetic diversity (AR: 1.57), while Ojo population had the largest population size (coverage: 275,736 m2) and the greatest level of genetic diversity (AR: 3.09). Hamdeok population showed evidence of genetic bottleneck. These results again suggest that Ne of Jeju populations is generally low (except Ojo population). Among Jeju populations, all pair-wise comparisons of FST values (i.e., degree of genetic differentiation) were highly significant (FST = 0.0612 ~ 0.7168, P < 0.001) despite Jeju populations that were geographically closely located, indicating that these local populations are genetically divergent, probably due to a lack of gene flow among the populations. The observed strong population structure was substantiated by evidence that five genetic clusters are most likely, based on population assignment test (STRUCTURE). The Mantel test showed a positive relationship between genetic distance (FST) and geographic distance (km) across all the populations sampled (R2 = 0.4118, P < 0.05), suggesting that our data follow Isolation By Distance (IBD) model. Woljeong population revealed the highest level of FST values compared to other populations within Jeju Island in IBD. STRUCTURE and factorial correspondence analysis (FCA) further showed that some Woljeong individuals included genotypes of Namhae populations. Population size of Woljeong (coverage: 310m2) was approximately 50 % smaller than that of Sungsan (coverage: 841m2); however, extent of its genetic diversity (AR: 2.39) was even higher than that of Sungsan population (AR: 1.77). We speculated that Woljeong population underwent a transplantation from Namhae populations with relatively higher level of genetic diversity. FST values within Namhae populations were relatively lower (compared to within Jeju Island) despite the populations that were geographically more distant. It means that level of gene flow is higher among Namhae populations than among Jeju populations. Z. marina is known to have different life histories by water depth. In subtidal zone (deep water depth) populations predominantly undertake sexual reproduction through seeds such as annual life history, whereas those of intertidal zone (shallow water depth) undertake both sexual and asexual reproductions through horizontal rhizomes i.e., perennial life history. STRUCTURE analysis showed no clear differences between shallow and deep populations at Namhae, but some FST values were statistically significantly different despite their low values. For Geoje population sampled in 2005, intertidal and subtidal populations were not significantly different (FST = 0.0045, P = 0.033), but these populations sampled in 2015 showed a significant difference (FST = 0.0328, P < 0.001). It means that genetic structure of Geoje has been changed over the 10 year period between shallow and deep populations. Overall, the Jeju and Namehae populations analyzed in the current study have relatively low levels of genetic diversity and distinct genetic compositions, which warns the message that this ecologically important species should be conserved separately in the local populations and with high priority. We propose that future conservation and restoration plans for seagrasses should consider genetic characteristics particularly because a close relationship between genetic diversity and ecological performance in marine species has been well documented.

농작물재해보험의 성과와 정책과제

최경환(Kyeong-Hwan Choi),채광석(Gwang-seok Chae),윤병석(Byeong-Seok Yoon) 한국농촌경제연구원 2010 한국농촌경제연구원 기본연구보고서 Vol.- No.-

잔티젠(Xanthigen)에 의한 고지방식이로 유도된 비만 개선 효과

최경미(Kyeong-Mi Choi),이윤선(Youn-Sun Lee),김원균(Wonkyun Kim),최영현(Yung-Hyun Choi),곽연길(Youn-Gil Kwak),정재철(Jae-Chul Jung),이정래(Jeongrai Lee),유환수(Hwan-Soo Yoo) 한국생명과학회 2012 생명과학회지 Vol.22 No.12

비만은 대사성 질환의 주요 위험 인자이다. 최근, 천연물질들의 비만 개선효과에 관심이 집중되고 있다. 잔티젠은 체중감소와 지질 대사 개선 효과가 있는 것으로 알려진 미역에서 유래한 fucoxanthin과 석류씨 오일에서 유래한 punicic acid로 구성된 복합 추출물이다. 본 연구에서, 우리는 C57BL/6N 마우스를 이용하여 잔티젠이 고지방식이로 유도된 비만을 개선시킬 수 있는지 조사하였다. 마우스는 각각 정상식이 대조군, 고지방식이 대조군, 고지방식이 + 1% 잔티젠군, 고지방식이 + 1% 녹차 추출물 양성대조군으로 나누어 11주간 사육하였다. 잔티젠 투여군은 고지방식이군과 비교하여 식이효율과 체중이 유의적으로 감소하였다. 체중 변화와 유사하게, 잔티젠은 복부부고환 지방조직과 후복막 지방조직 및 간의 무게를 고지방식이군 대비 뚜렷하게 감소시켰고, 혈청 LDL-콜레스테롤 함량 또한 유의적으로 감소시켰다. 이러한 결과들은 잔티젠의 탁월한 항비만 효과를 갖는 건강기능식품 소재로서의 개발 가능성을 제시한다. Obesity is a risk factor for numerous metabolic diseases. Recently, naturally occurring compounds that may improve obesity have received increasing attention. Xanthigen is a mixture of fucoxanthin and punicic acid derived from brown seaweed and pomegranate seed, respectively, which have been traditionally used for lipid-lowering effects in humans. In this study, we investigated whether Xanthigen attenuates high-fat diet-induced obesity in C57BL/6N mice. The mice were fed on a normal diet (ND), high-fat diet (HFD), HFD plus 1% Xanthigen or HFD plus 1% green tea extract (GTE) for 11 weeks. Food efficiency ratio (FER) and body weight were significantly reduced in mice fed HFD plus Xanthigen compared to HFD-fed mice. Consistent with the results in body weight change, Xanthigen also significantly decreased the weights of epididymal adipose tissue, retroperitoneal adipose tissue, and liver in HFD plus 1% Xanthigen-fed mice. The serum level of low-density ipoprotein (LDL)-cholesterol was significantly decreased in HFD plus Xanthigen-fed mice compared to HFD-fed mice. These results suggest that Xanthigen may be useful in the development of a functional health food for anti-obesity.