잔티젠(Xanthigen)에 의한 고지방식이로 유도된 비만 개선 효과

최경미(Kyeong-Mi Choi),이윤선(Youn-Sun Lee),김원균(Wonkyun Kim),최영현(Yung-Hyun Choi),곽연길(Youn-Gil Kwak),정재철(Jae-Chul Jung),이정래(Jeongrai Lee),유환수(Hwan-Soo Yoo) 한국생명과학회 2012 생명과학회지 Vol.22 No.12

비만은 대사성 질환의 주요 위험 인자이다. 최근, 천연물질들의 비만 개선효과에 관심이 집중되고 있다. 잔티젠은 체중감소와 지질 대사 개선 효과가 있는 것으로 알려진 미역에서 유래한 fucoxanthin과 석류씨 오일에서 유래한 punicic acid로 구성된 복합 추출물이다. 본 연구에서, 우리는 C57BL/6N 마우스를 이용하여 잔티젠이 고지방식이로 유도된 비만을 개선시킬 수 있는지 조사하였다. 마우스는 각각 정상식이 대조군, 고지방식이 대조군, 고지방식이 + 1% 잔티젠군, 고지방식이 + 1% 녹차 추출물 양성대조군으로 나누어 11주간 사육하였다. 잔티젠 투여군은 고지방식이군과 비교하여 식이효율과 체중이 유의적으로 감소하였다. 체중 변화와 유사하게, 잔티젠은 복부부고환 지방조직과 후복막 지방조직 및 간의 무게를 고지방식이군 대비 뚜렷하게 감소시켰고, 혈청 LDL-콜레스테롤 함량 또한 유의적으로 감소시켰다. 이러한 결과들은 잔티젠의 탁월한 항비만 효과를 갖는 건강기능식품 소재로서의 개발 가능성을 제시한다. Obesity is a risk factor for numerous metabolic diseases. Recently, naturally occurring compounds that may improve obesity have received increasing attention. Xanthigen is a mixture of fucoxanthin and punicic acid derived from brown seaweed and pomegranate seed, respectively, which have been traditionally used for lipid-lowering effects in humans. In this study, we investigated whether Xanthigen attenuates high-fat diet-induced obesity in C57BL/6N mice. The mice were fed on a normal diet (ND), high-fat diet (HFD), HFD plus 1% Xanthigen or HFD plus 1% green tea extract (GTE) for 11 weeks. Food efficiency ratio (FER) and body weight were significantly reduced in mice fed HFD plus Xanthigen compared to HFD-fed mice. Consistent with the results in body weight change, Xanthigen also significantly decreased the weights of epididymal adipose tissue, retroperitoneal adipose tissue, and liver in HFD plus 1% Xanthigen-fed mice. The serum level of low-density ipoprotein (LDL)-cholesterol was significantly decreased in HFD plus Xanthigen-fed mice compared to HFD-fed mice. These results suggest that Xanthigen may be useful in the development of a functional health food for anti-obesity.

역상HPLC컬럼을 이용한 생체 내 단당세라마이드 분석

최미화(Mi-Hwa Choi),최경미(Kyeong-Mi Choi),지소영(So-Young Ji),이윤선(Youn-Sun Lee),조주현(Ju-Hyun Cho),이용문(Yong-Moon Lee),윤여표(Yeo-Pyo Yun),유환수(Hwan-Soo Yoo) 대한약학회 2010 약학회지 Vol.54 No.5

Glycosphingolipids are structural components of mammalian cell membranes and are involved in essential cellular physiology such as cell-cell interaction, recognition, transmembrane signaling, proliferation and cell death. In this study, the simple quantitative method of monoglycoceramides-containing glucosylceramide and galactosylceramide was developed. The glycosylceramides extracted from culture cells and rat plasma were resolved by TLC, deacylated by SCDase and analyzed by HPLC-fluorescence detector at an excitation wavelength of 340 nm and an emission wavelength of 455 nm. Limit of detection was approximately 0.1 pmol and limit of quantification was about 1 pmol for both monoglycoceramide standards. The recoveries of standard glucosylceramides from intra- and inter-day assays were 113.8 and 88.8% and those of galactosylceramides were 110.7 and 123.9%, respectively. The monoglycoceramide contents of SW-620 cells and rat plasma were 141.5±5 pmol/1×106 cells and 3.9±0.3 μM, respectively. The present analytical method provides a reproducible quantification and total content of monoglycoceramide which may be as a potential biomarker for lipid imbalance-related human diseases.

Allium Hookeri Extract Enhances Glucose Uptake through GLUT4 Up-regulation in 3T3-L1 Cells

Young Eun Kang(강영은),Kyeong-Mi Choi(최경미),Eunjin Park(박은진),Won-Beom Jung(정원범),Heejin Jeong(정희진),Hwan-Soo Yoo(유환수) 한국생명과학회 2017 생명과학회지 Vol.27 No.3

삼채(Allium species)는 전통적인 약재나 건강 증진 식품으로 사용되어 왔다. 특히, Allium hookeri (A. hookeri)는 제 2형 당뇨병 모델 마우스에서 혈당 감소 효과가 보고되었다. 본 연구에서는 A. hookeri 추출물이 3T3-L1 세포에 서 인슐린 민감성을 증진시키는지 시험하였다. 3T3-L1 지방세포분화가 불완전하게 유도되는 저농도의 인슐린 조건에서, A. hookeri 추출물은 세포 내 지방 함량을 증가시키고, 분화 유도 전사인자인 PPARγ의 발현을 상승시켰다. 또한, A. hookeri 추출물은 포도당 수송체 4(GLUT4)의 발현을 증가시킴으로써 세포 내 포도당 흡수(glucose uptake)를 향상시켰다. 이러한 결과들은 A. hookeri 추출물이 인슐린 민감성을 증진시켜 PPARγ와 GLUT4를 활성화하고, 세포 내 포도당 흡수를 촉진한다는 사실을 보여준다. 따라서, A. hookeri 추출물은 당뇨병의 예방 및 치료에 임상적으로 응용될 수 있을 것으로 생각된다. Diabetes mellitus is associated with insulin resistance, which leads to down-regulation of insulin signaling and the decreased glucose uptake. Adipocytes are sensitive to insulin, and closely implicated in insulin resistance and diabetes. Insulin stimulates differentiation of preadipocytes to adipocytes, and increases glucose transport. Allium species have been used as traditional medicine and health-promoting foods. Allium hookeri (A. hookeri) is reported to improve the pancreatic β-cell damage and exhibit pancreatic anti-inflammatory activity in streptozotocin-induced diabetic rats. We investigated whether A. hookeri extract (AHE) may stimulate glucose uptake in adipocytes through increasing insulin sensitivity. AHE enhanced fat accumulation, a differentiation biomarker, under the partial induction of differentiation by insulin. PPARγ, a transcription factor highly expressed in adipocytes, promotes adipocyte differentiation and insulin sensitivity. AHE increased the differentiation of preadipocytes through up-regulation of PPARγ. The activation of PPARγ increases the GLUT4 expression during adipocyte differentiation. GLUT4 is responsible for glucose uptake into the adipocytes. AHE increased the expression of GLUT4 in adipocytes, and subsequently enhanced the insulin-stimulated glucose uptake. These results suggest that AHE promotes adipocyte differentiation through activation of PPARγ, and leads to enhance glucose uptake in adipocytes along with GLUT4 up-regulation. Thus, AHE may be effective for the insulin-sensitizing and anti-diabetic activities.

신경성 염증질환에서 지질 신호전달물질인 lactosylceramide의 역할

이윤선, 유재명, 최경미, 이용문, 홍진태, 윤여표, 유환수 충북대학교 약품자원개발연구소 2007 약학논문집 Vol.22 No.-

-본문참고-

Obesity as a primary etiology of diabetes

최경미, 우환수 충북대학교 약품자원개발연구소 2014 약학논문집 Vol.29 No.-

Obesity and diabetes are chronic metabolic disorders and their prevalence rates have been increased globally. Obesity is strongly related to development of diabetes which occurs based on the lack of insulin secreted from β-cells of the pancreas and/or inability of the body to respond to insulin, leading to hyperglycemia. As young children gain more weight, diabetes can be diagnosed earlier. More weight increases insulin resistance, leading to the development of diabetes in individuals who are predisposed genetically to diabetes. In order to develop insulin resistance, leading to diabetes, β-cells should not be able to compensate for decreased insulin sensitivity. The critical factor affectiong insulin sensitivity is the nonesterified fatty acids (NEFAs) that are secreted from adipose tissue in obese people. A factor that may contribute to a loss of β-cells function is an elevation of plasma NEFAs levels. Weight gain and increased body mass play a critical role in the elevation of diabetic population globally. Thus, new approaches in managing and preventing diabetes in obese individuals must be pursued.

Adipocyte differentiation as a mechanistic process of obesity

최경미, 이주현, 이안나, 김길용, 강영은, 정재철, 윤여표, 요환수 충북대학교 약품자원개발연구소 2013 약학논문집 Vol.28 No.-

Obesity is characterized by excessive growth and expansion of adipose tissue which can be expanded by adipocyte hyperplasia and hypertrophy, and secretes leptin and adiponectin. Adipocyte differentiation induces enhancement of lipogenic capacity, the formation of lipid droplets, and gain of insulin sensitivity. Obesity is implicated in diabetes, hypertension, coronary heart disease, and cancer. At present, orlistat and sibutramine were approved for long-term use. Orlistat, a gastric and pancreatic lipase inhibitor, has some side effects such as gastrointestinal disorders, fatty and oily stool, and an increased risk of colon cancer and kidney stones. Sibutramine mainly inhibits the appetite, and stimulates thermogenesis to increase energy expenditure. However, sibutramine was also shown to have common side effects including dry mouth, headache, insomnia, and an increased risk of developing heart problems. The development of safe and efficient anti-obesity drug is demanded and the adipocyte differentiation may be an attractive target.

비타민 C 병용에 의한 흰쥐 혈중 루테인 함량 증가와 배양세포에서의 항산화 효과

이승현, 이윤선, 최경미, 신동미, 이용문, 윤여표, 유환수 충북대학교 약품자원개발연구소 2011 약학논문집 Vol.26 No.-

Lutein functions as an antioxdant that scavenges reactive oxygen species, and its consumption is inversely related to eye diseases such as age-related macular degeneration (AMD) and cataracts. This is supported by the finding that lutein and zeaxanthin (a stereo isomer) are concentrated in the lens and macula lutea, an area of the retina responsible for central and high acuity vision. The purpose of this study was to investigate whether lutein absorption may be modulated, and its antioxidant activity be improved by combining with vitamin C. Lutein content was determined by HPLC. The combination of lutein with vitamin C increased the serum levels of lutein in SD rats, and inhibited malondialdehyde (MDA) formation, indicating that antioxidant activity of lutein combined with vitamin C appears to be improved by increasing lutein content. These results suggest that the increased serum levels of lutein may be associated with improving AMD and cataracts in humans through the pathogenesis of antioxidant mechanism.