정자기장이 MC3T3-E1 세포의 골결절형성에 미치는 영향

김광덕,권오원,류현모 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.1

정자기장이 MC3T3-E1 세포의 골생성능력에 어떠한 영향을 미치는지 알아보기 위하여 MC3T3-E1 세포를 4well세포배양접시에 SmCo5 영구자석을 이용하여 76.4mT의 정자기장을 가한 상태에서 5일, 7일, 11일, 15일 및 21일간 배양한 후 세포의 ALP 활성도를 측정하고 Alizarin red S 염색을 통하여 골결절양상을 관찰하여 다음과 같은 결과를 얻었다. ·정자기장을 가한 군중 7일군, 11일군 및 15일 군에서 MC3T3-E1 세포의 ALP활성도가 대조군에 비해 낮게 나타났으며(P<0.01) 그중 11일군에서 가장 억제효과가 많았다. ·Alizarin red S 염색을 통한 골결절 양상은 11군까지는 실험군과 대조군 모두에서 염색양상이 나타나지 않았고 15일 군에서 대조군은 소량의 골결절이 염색되었으며 실험군은 골결절 염색이 되지 않았으며 21일 군에서는 실험군과 대조군 모두에서 골결절이 염색되었으나 대조군에서 약간 더 염색된 양상을 나타내었다. To evaluate the effect of a static magnetic field on the bone producing potential of MC3T3-E1 cells, the alkaline phosphatase activity was measured after the cells having been cultured under 76.4mT static magnetic field using a SmCo5 magnets for 5days, 7days, 11days, 15days and 21 days for each cell culture group. Also, the amount of bone nodule stained with Alizarin red S was observed. The results were as follows . The alkaline phosphatase activity of the 7, 11, and 15 days group among the experimental groups was decreased as compared with the control groups, and the decrease of alkaline phosphatase activity in the 11 days group was the most evident among them. . Any stained bone nodules of both groups had not been observed until the 11th day. The stained bone nodules in the control groups were found on the 15th day, but not in the experimental groups. The stained bone nodules were observed in both groups on the 21st day, but the control groups have more bone nodules than the experimental groups.

황토 급여가 거세한우의 발육에 미치는 영향

조원모,기광석,정하연,강수원,김준식,백봉현,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

본 연구는 거세한우 육성우에 대한 황토 첨가급여가 발육에 미치는 효과를 구명하고자 발육능력, 사료섭취량을 규명한 바 아래와 같은 결과를 얻었다. 전 시험기간 동안의 평균 일당증체량은 대조구와 황토급여구가 각각 0.91㎏과 0.87㎏/day로 대조구가 높은 것으로 나타났지만 유의성은 인정되지 않았다. 사료섭취량은 대조구와 황토급여구 모두 평균 일일 두당 농후사료 섭취량이 2.42㎏이었으나, 건초섭취량에 있어서는 각각 3.02와 3.14㎏으로 황토급여구가 다소 높은 경향으로 나타났다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the growth performance in growing Hanwoo steers. A total of 8 calves was allocated into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitim Reddish clay) for Hanwoo steers. This study was carried out for 5 months with a total of 8 calves. The results obtained in the present studies are summarized as follows : Average daily gains throughout the experimental period of 4 months to 9 months of age were 0.91 and 0.87㎏ for control group and reddish clay-supplemented group, respectively, however, no statistical significances were found. Average daily intake of concentrates was 2.42㎏ for each group, while that of hay tended to be higher in reddish clay-supplemented group(3.14㎏) than in control group(3.02㎏).

캔음료중 epichlorohydrin 의분석법에 관한 연구

이광호,곽인신,최재천,전대훈,김형일,강경모,최병희,김귀정,이철원 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

캔식품중에 잔류하는 epichlorohydrin을 분석하기 위한 pu링e & trap-Cc/uf통D 분석법을 확립하여 캔의 lacauer 코팅제에서 식품으로 이행되어 잔류하는 epichtorohydfn을 측정하고, 식품유사용매에 의한 용출시험을 통해 일일추정섭취량을 산출하였다. epichlorohydrin의 검량선은 y=0.0006x+0.OO6?(R큰0.9983)의 직선성·라 0.05#ga의 검출한계를 나타내었다. 각종 음료 및 식품유사용매에 일정량의 epichlorohydrin을 첨가한 후 분석하였을 때 회수을은 72~91%였으며, 실제 시판되는 f5건의 캔음료 중 잔류하는 epichlorohydrin을 측정한 결과 모든 캔음료에서 검출한계(0.1#94) 이하였다. 제관업체에서 구입한 modifed epoxy, epoxy phenolic, vinyl organosol, epoxy phenol ester 등의 BADGE(bisphenol A diglycidyt ether)형 에폭시수지로 lacquer 코팅된 식품용 캔에 식품윤사용매인 10% 또는 95% 에탄올을 층L진한 후 90~121'c의 드라이오븐에서 30분~2시간동안 용출시켰을 패 캔의 lacquer 코팅재에서 식품유사용매로 이행되는 epichBorollydirn은 코팅재질, 그리고 가열온도 및 시간에 문관하게 모든 캔에서 검출한계(0.4#ga)이하 였다. 위의 용출결과를 FD.f_의 "Recommendation for Chemistry Data for Indirect Food Additive Petition" 윽 일일추정섭취량 산출법에 적용하여 얻은 epichtorohydrin의 일딜추정 섭 취 량은 0.2r조persorday이하였다. A sensitive ana1?~7tical method based on gas cl)romatogFapy~mass spectrometry with a selected ion monitoring (GE/hfS-Slhf) and the purge-and-trap concentrator ))·asdeveloped for determining ot'epicillorohydrin in canr;ed be)#erages coated with eporl· resin. Thecalibration rurve in the ranre of 0.j ~50nr had'correlation coefficient of 0.9983 and detection limit of 0.Osrga. Recoveries of epichlorohydrin spiked to beverages were in the range of 72 ~91% with detection limits of 0.1 #ga. fn survey of epichlorohydff in twenty commercialcanned beverage samples, epichlorohydrin was not found in all the samples with the detectionlimit of 0.1 r9a. In migration test of various cans coated with BADGE-based coating cans;modifed epoxy, epoxy phenolic, vinyl organosol, epox?· phenol ester were exposed to the foodsimulants of 10% ethanol and 95% ethanol. flfo delectable epichlorohydrin migrated to foodsiHulants was found in aTl the cars with detection Jimit of 0.4 #9/L. Using the miEration data,along uTith the applicstion of food and beverage cans complied with FDA's "Recommendationfor Chemlstry Data f,3r Indirect Food Additive Petition" , the potential dietary exposure toepichlorohydrin was estlmated to be under 0.2rf/person/day.

한우에 있어 황토 첨가급여가 면역기능에 미치는 영향

조원모,기광석,정하연,강수원,김준식,오성종,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.2

본 연구는 한우 육성우에 대한 황토 첨가급여가 항병성에 미치는 효과를 구명하고자 혈액성상변화를 규명한바 아래와 같은 결과를 얻었다. 대조구 및 처리구에서 전체적인 혈액성상의 변화는 없었으나 백혈구수치가 대조구 및 처리구에서 각각 15.35K/㎕ 및 13.37K/㎕이였으며, 적혈구에 있어서도 정상수치인 5~10M/㎕보다 대조구 10.03M/㎕, 처리구 11.35M/㎕로 모두 높은 수치를 보였다. 황토첨가에 따른 혈액성상의 변화는 처리간에 유의한 차이는 보이지 않았다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the immunity of growing Hanwoo steers. A total of 8 calves were divided into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitu reddish clay) for Hanwoo steers. This study was carried out for 5 months using 8 calves. The results obtained by the present study are summarized as follows : The proportion of WBC, NE as well as LY(15.35, 6.85, 6.86K/??) was higher in the control group than in the Reddish clay-supplemented group. But no statistical differences were found in blood substances among treatment calves.

線型 位相 特性을 지닌 多重 노치필터의 具現

고광식,박용완,김영모,이건일 경북대학교 공과대학 1985 工大硏究誌 Vol.14 No.-

The purpose of this paper is to advocate a simple approach for realizing a real time linear-phased multiple notch filter(MNF) using a modified concept of the MFIR(multiplicative finite impulse response) filter. It is shown that the proposed MNF is quite effective for eliminating low frequencies and their harmonics, and also for offering an economical solution because it requires no multipliers and uses limited adders and memories. In this paper, we will describe the theoretical MNF approach, the hardware, the software and the noise analysis of the proposed MNF.

물류 원가 ABC 시스템을 이용한 다기준 활동 원가 분석 연구

양광모,김창식,박재현,강경식 한국물류학회 2006 물류학회지 Vol.16 No.1

목재의 열화 탐지를 위한 현장용 CT 개발

이전제,김광모 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The main purpose of this study is the development of potable CT systems which can be used to evaluate the structural safety of member in ancient wood buildings. Many researches about non-destructive evaluation(NDE) of wood have been performed. But most of these researches were focused on inspection of wood whether it had some deteriorated parts or not. In this study, CT systems were developed for quantitative detection of wood deterioration. And, the developed systems were composed of portable devices to apply for ancient wood buildings in field. Ultrasonic wave and X -ray CT methods were considered. And 5 circular shape specimens were tested for verification of developed methods. Over 23mm defects could be detected by ultrasonic CT method, but the size, shape and position did not agree with prototype. When same specimens were tested by X-ray CT method, the position and size of defects could be detected exactly.

흰쥐에서 음경발기 평가의 척도로서 음경해면체내압측정술 확립

송윤섭,김용준,이광우,김준모,문기혁,박영호,민영기,유형균,김형건 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Because studies about the control of penile erection at the entral nervous system was dependent on the animal experiments, establishment of measuring penile erection is important to evaluate the effects changes at the central nervous system on the penile erection. Intracavernous pressure measurement in rats has been introduced as a suitable index for the evaluation of penile erection but this method is invasive and technically difficult. So, we established the intracavernous pressure measurement in rats as the experimental index for penile erection. Materials and Methods: 42 male adult Sprague-Dawley rats (250-300gm) were divided to saline or papaverine treated group. Rats were placed on a heating table to maintain their body temparature and anesthetized with 50mg/kg of pentobarbital sodium by intraperitoneal injection. A cannula was inserted to the left carotid artery to measure systemic blood pressure. Saline (0.05, 0.1ml), papaverine(0.05, 0.1, 0.25, 0.5, 1.0, 2.0mg) were injected intracavernously via 26 guage needle filled with saline or papaverine which connected to pressure transducer and polygraph was inserted into the corpus cavernosum on one side to inject the drug and to measure intracavernous presure. Results: Properly executed insertion and intracavernous administration produced a instantaneous but transient rise in intracavernous pressure that substantially stabilized and maintained at 5.4±0.4mmHg. Intracavernous pressure and duration of penile blood flow following intracavernous injection of papaverine are increased comared to those of saline. Conclusions: Monitoring intracavernous pressure in rats represents a suitable index for the evaluation of penile erection in small laboratory animals.

집성재 단면구성에 따른 휨성능 평가

이전제,김광모,한재수,김재건 한국목재공학회 2003 목재공학 Vol.31 No.5

본 연구에서는 몇 가지 새로운 집성재 단면구성 방법을 제안하고, 국산 낙엽송과 합판으로 제조된 집성재의 휨시험을 통해 단면 구성에 따른 집성재의 침성능을 평가 비교하였다. 수평적층(BH), 수직적층(BH), 수직으로 합판보강된 수직적층(BVV)과 수평으로 합판보강된 수직적층(BVH)의 총 네가지 형태의 단면구성 방법에 대하여 실험을 실시하였다. 파괴모드를 관찰하고, 실험을 통해 얻어진 하중-변형 곡선으로부터 MOR 및 MOE를 계산하여 이를 바탕으로 각 단면구성 방법의 휨성능 개선효과를 비교하였다. MOR의 경우에는 결함의 분산으로 인해 수직적층에서 수평적층에 비해 23% 정도의 개선효과를 얻을 수 있었으나, MOE의 경우에는 거의 유사한 값을 나타내었다. 수평으로 합판보강된 경우에는 상대적으로 휨성능이 약한 합판이 사용됨에 따라 MOE가 15% 정도 감소하였으나, 수직으로 합판보강된 경우에는 합판이 사용되었음에도 불구하고 MOE의 감소 없이 MOR이 개선되는 효과를 나타내었는데 이는 전단보강의 효과로 생각되며 이러한 효과를 파괴모드의 관찰을 통해서도 확인하였다. 이상의 결과로부터 집성보의 단면 구성시 라미나의 적절한 배치 및 전단보강 등을 통하여 전체적인 휨성능을 향상시킬 수 있음을 확인하였다. In this paper, bending capacities of glulams with different configurations of cross-section were evaluated. These configurations included horizontal(BH), vertical(BVN), vertical with vertical plywood(BVV) and vertical combination of lamination with horizontal plywood(BVH). Full-scale bending tests were performed to investigate the effect of different section configurations on bending strength(MOR) and stiffness(MOE) of glulam. Compared with type BH, MOR of glulam with type BVN configuration was improved about 23%, which was considered to be caused by defect dispersion effect, while MOE of glulams with these two types of configurations were similar. Because MOE of plywood is generally smaller than that of solid wood laminar, MOE of type BVH glulam decreased about 15%, but in the case of type BVV glulam, MOE was improved without any reduction of MOE. The reason of this result could be undersood in the view of shear-reinforcement effect, which was verified from analysis of fracture mode. From the results of this study, it was concluded that bending capacity of glulam could be improved by proper section design, such as laminar arrangement and shear reinforcement.

미끄럼 防止 施設 과 車輛走行特性間의 關係

이종달,김원한,정광수,강창모 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1

This study analyzes the problems about the skid-proof pavement on the curved section in moistening by using PC-Crash, a program for traffic accident reconstruction. 1.As a result of applying to the overall treatment gap type pavement method of construction, it showed that the result was considerably stabilized as 4.47% decreased at the average braking distance and 12.66% decreased at the average lateral skid distance. 2.The overall treatment grooving method showed excellent effects as 3.23% decreased at the average braking distance and 9.42% decreased at the average lateral skid distance compared with the general overall treatment method.