소고기 대체의 관점으로 분석한 1인 가구 소비자의 비육류 단백질 식품 구매: 연령구간별 비교를 중심으로

홍윤지,김사랑 (사)한국조리학회 2022 한국조리학회지 Vol.28 No.7

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The fate of targets that become invisible by motion-induced blindness

홍윤지 한국감성과학회 2017 춘계학술대회 Vol.2017 No.-

In motion-induced blindness (MIB), salient targets, imposed on certain global moving patterns, are reported by observers to have disappeared form their conscious awareness (Bonneh, Cooperman, & Sagi, 2001). Previous studies on MIB have extensively discussed on the cause for Such phenomenon (Bonneh et al., (2001): Graf, Adams, & Lages, 2002: Hsu, Yeh & Kramer, 2004). However, it has yet been discussen on the fate of target that gets reported to have disappeared. The current study focused on examining the fate of the targets that experienced MIB by utilizing an explicit memory task (Experiment 1) and an implicit memory task (Experiment 2). The experiment was designed to be conducted in two different phases-MIB-task phase and Memory test phase. Using two-syllable Korean words as target stimuli, participants were Instructed to report the disappearance of the targets by a keypress while maintaining their eyes on The fixation point that was presented on the center of the display. Upon finishing MIB-task phase, Participants performed a target recognition task, a task added to evaluate the difference in a Performance level between targets that experienced MIB (MIB condition) and targets that were physically removed from the display (control condition). Comparing sensitivity index (d`), a tendency was found for MIB-condition targets to be remembered less, compared to those in control condition-though it fell short of statistically significant level. To further examine the impact of MIB on target representations, a lexical decision task was performed in Experiment 2, comparing the difference in performance level between target conditions using an implicit memory task. Both explicit and implicit momory task performance will be compared to further evaluate how does MIB impact the changes in target representations.

심부전 환자에서 수혈관련급성폐손상의 진단 1예

홍윤지,김정아,최규태,이경훈,박경운,송정한,한규섭 대한수혈학회 2011 大韓輸血學會誌 Vol.22 No.3

A 71-yr old man with known coronary heart disease complained of dyspnea and severe sweating one hour after transfusion of one unit of packed Red Blood Cells (pRBC). Although the heart failure was secondary to the remote acute myocardial infarction, except inflammatory lesion in his toes, he had remained asymptomatic for a long time. Observed as having clear lungs a few hours before transfusion, the patient suffered an acute hypoxic episode (SpO2=61%) and a resulting chest x-ray revealed bilateral pulmonary infiltrates. Confused as the cause of the acute deterioration, he was transferred to the intensive care unit and received managed lung care by mechanical ventilation as well as other conservative care methods. Two days after the acute hypoxic event there was apparent clinical improvement, and he was weaned from ventilator support. His amelioration resulted in subsequent diagnosis of Transfusion-Related Acute Lung Injury (TRALI). TRALI is underdiagnosed in patients due to its nebulous nature. Evaluating patients exhibiting symptoms of bilateral lung infiltrate after blood transfusion for TRALI, and subsequent reporting of the diagnosis results, will help reveal the actual frequency of incidence of TRALI, and prevent additional events by tracing the blood donor.

A Case of Imported Plasmodium malariae Malaria

홍윤지,양선영,이경훈,김택수,김홍빈,박경운,송정한,김의종 대한진단검사의학회 2012 Annals of Laboratory Medicine Vol.32 No.3

Malaria, the most common vector-borne parasite infection worldwide, results from infection by Plasmodium species. Approximately 80% of malaria cases are caused by P. vivax, which is broadly distributed from tropical to temperate regions; P. falciparum is the second most common infectious species. P. malariae and P. ovale are responsible for a relatively small proportion of malaria cases. Here, we report the case of a 23-yr-old Korean woman who acquired a P. malariae infection while visiting the Republic of Ghana in West Africa for business. She was diagnosed with P. malariae malaria on the basis of peripheral blood smear (PBS) and species-specific conventional and real-time PCR assays for 18S rRNA. She was treated with hydroxychloroquine, and the resulting PBS examination on day 2 suggested that negative conversion occurred. At her 1-month follow-up, however, both the PBS examination and molecular test for malaria demonstrated recurrent parasitemia. We started rescue therapy with mefloquine, and the patient recovered successfully. This is an important finding suggesting possible late recrudescence of a chloroquine-resistant P. malariae strain identified not only by its morphological features, but also by molecular tests.

다중단일염기시발체확장반응을 이용한 한국인에서의 혈소판항원검사

홍윤지,박경운,장호은,정유선,이화진,현정원,황상미,김택수,송정한,한규섭 대한수혈학회 2013 大韓輸血學會誌 Vol.24 No.2

Background:Alloimmunization of human platelet antigens (HPA) is associated with clinically significant disease, such as platelet refractoriness, neonatal alloimmune thrombocytopenia, or posttransfusion purpura. It is determined by single nucleotide polymorphism of genes for platelet membrane glycoprotein. To date, approximately 27 HPAs have been discovered, and their frequencies differ depending on ethnicity and country. Methods:We conducted an investigation of prevalence of HPA in the Korean population using a multiplex single-base primer extension reaction (SNaPshot). With 84 specimens from healthy donors, HPA genotyping was performed on 11 different HPAs, including HPA-1, -2, -3, -4, -5, -6, -7, -8, -9, -13, and -15. Results:A total of 90 blood samples were genotyped. The genotype frequencies of HPA were as follows: HPA-1a/1a: 100.0%, -2a/2a: 83.3%, -2a/2b: 14.3%, -2b/2b: 2.4%, -3a/3a: 39.3%, -3a/3b: 52.4%, -3b/3b: 8.3%, -4a/4a: 100.0%, -5a/5a: 95.2%, -5a/5b: 4.8%, -6a/6a: 94.0%, -6a/6b: 6.0%, -7a/7a: 100.0%, -8a/8a: 100.0%, -9a/9a: 97.6%, -9a/9b: 2.4%, -13a/13a: 100.0%, -15a/15a: 23.8%, -15a/15b: 51.2%, and -15b/15b: 25.0%. Conclusion:The SNaPshot assay was employed for detection of SNPs in various clinically significant HPA genes. In addition to well-known frequencies of previously reported HPA-1 to -8, this study showed frequencies of HPA-9, -13, and -15 in Koreans for the first time. The SNaPshot technique might be suitable for use in actual clinical testing in patients with platelet alloimmunization. 배경: 사람혈소판항원(human platelet antigen, HPA)과 관련된 동종면역은 혈소판불응증, 신생아동종면역혈소판감소증, 수혈후자반과 같은 다양한 임상질환을 일으킨다. 이는 주로 혈소판막의 당단백 발현에 관여하는 유전자의 단일염기다형성(single nucleotide polymorphism, SNP)에 의해결정된다. 현재까지 약 27개의 사람혈소판항원이알려졌고, 그 빈도는 인종에 따라 다르다. 방법: 다중단일염기시발체확장반응(multiplex single- base primer extension reaction, SNaPshot)에 기반한 혈소판항원의 유전형분석기법을 통해 한국인에서의 사람혈소판항원의 빈도를 알아보았다. 신생아동종면역혈소판감소증 의심환아의 부모와 건강한 헌혈자로부터 얻어진 90 검체에 대해유전형분석을 시행하여 HPA-1, -2, -3, -4, -5, -6, -7, -8, -9, -13, -15를 확인하였다. 결과: 90 검체에 대해 유전형분석이 시행되었다. 혈소판항원의 유전형분석의 결과에 따르면HPA-1a/1a: 100.0%, -2a/2a: 83.3%, -2a/2b: 14.3%, -2b/2b: 2.4%, -3a/3a: 39.3%, -3a/3b: 52.4%, -3b/3b: 8.3%, -4a/4a: 100.0%, -5a/5a: 95.2%, -5a/5b: 4.8%, -6a/6a: 94.0%, -6a/6b: 6.0%, -7a/7a: 100.0%, -8a/8a: 100.0%, -9a/9a: 97.6%, -9a/9b: 2.4%, -13a/13a: 100.0%, -15a/15a: 23.8%, -15a/15b: 51.2%, and -15b/15b: 25.0%이었다. 결론: 임상적 의의가 명확한 대표적인 혈소판항원의 유전형을 신속하고 정확하게 분석하기 위해 분자진단기법인 다중단일염기시발체확장반응을 적용하였다. HPA-1부터 -8의 한국인에서의빈도는 기존에 알려진 빈도와 유사한 결과를 나타내었고, -9, -13, -15의 빈도는 이번 연구를 통해처음으로 확인할 수 있었다. 다중단일염기시발체확장반응을 통한 혈소판항원의 유전형분석기법은 향후 혈소판불응증 및 신생아동종면역혈소판감소증 등의 원인을 규명하기 위한 실질적인 임상검사로 사용될 수 있을 것이다.

급성골수백혈병 환자에서 발견된 자가 항-M 항체 증례 보고

홍윤지,김진석,육희정,정유선,김형석,황상현,오흥범,고대현 대한진단검사의학회 2023 Laboratory Medicine Online Vol.13 No.1

The anti-M antibody is a naturally occurring antibody and generally active below 37˚C, with optimum activity at 4˚C. Since auto-anti-M antibody was first reported in 1970, twenty cases have been described. The auto-anti-M antibody could be presented with a negative direct antiglobulin test result and not be associated with anemia or adverse symptoms. Therefore, it is likely to be missed during the routine serologic procedure. Here, we report the first case of auto-anti-M antibody in Korea. A 73-year-old male with relapsed acute myeloid leukemia was admitted with general weakness and atypical pneumonia. The underlying disease created a need for frequent transfusions due to anemia and thrombocytopenia. In the pre-transfusion test, unexpected and not previously seen antibodies were identified as anti-M antibodies and confirmed. The auto-control result was positive. Antigen tests were performed for the antibody’s accurate specification. Finally, it was confirmed that the patient’s red blood cell phenotype had an M antigen and was reported as an auto-anti-M antibody. To our knowledge, this is the first auto-anti-M antibody case in Korea and the Asian region. 항-M 항체는 주로 자연발생적인 항체로 37˚C 이하에서 반응하며 최적반응 온도는 4˚C인 것으로 알려져 있다. 자가 항-M 항체는 1970년에 처음 보고된 이래로 지금까지 20명의 사례만 보고되어 있다. 이는 자가 항-M 항체가 직접항글로불린검사에서 대부분 음성이며 심각한 임상증상을 유발하지 않기 때문에 통상적인 검사에서 발견되기 힘들기 때문인 것으로 여겨지고 있다. 저자들은 한국에서 처음으로 발견된 자가 항-M 항체의 증례를 보고하고자 한다. 재발한 급성골수백혈병으로 항암치료를 받고 있는 73세 남성이 지속되는 전신쇠약증과 비정형폐렴 소견으로 내원하였다. 기저질환으로 인한 혈색소 감소와 혈소판 감소로 지속적으로 수혈을 받아왔다. 입원 후 수혈전검사에서 이전에는 나타나지 않았던 비예기항체가 확인되어 동정검사를 진행하였다. 항-M 항체와 한랭항체가 확인되었고 자가 대조검사에서 양성반응을 보였다. 정확한 동정을 위해 항혈청시약을 이용한 검사를 진행하였다. 환자의 적혈구 표현형이 M 항원을 가지는 것을 최종 확인하여 자가 항-M 항체로 보고하였다. 본 증례는 한국뿐 아니라 아시아 권역에서 최초로 확인된 자가 항-M 항체에 대한 보고이다.

논문(論文) : 《삼국연의(三國演義)》관도지전(官渡之戰)에 나오는 전투도구와 시설물에 관한 고찰

홍윤지 ( Youn Ki Hong ) 중국어문연구회 2010 中國語文論叢 Vol.0 No.45

고역가, 저결합활성을 지닌 항-JMH 항체가 검출된 1예: 국내 첫 보고

김태열,홍윤지,김형석,박경운,한규섭 대한수혈학회 2017 大韓輸血學會誌 Vol.28 No.2

Anti-John Milton Hagen (JMH) is a high-titer, low-avidity (HTLA) antibody against the high frequency red blood cell (RBC) antigen JMH. It occurs very rarely and has not yet been reported in Korea. Here, we report a case of anti-JMH antibody identified in a 92-year-old man without previous blood transfusion history, who had been hospitalized with pneumonia. The patient’s hemoglobin level was reduced to 7.6 g/dL on the 35th day of hospitalization, requiring RBC transfusion. Antibody identification test revealed antibodies that showed pan-reactivity to all panel cells at the antiglobulin phase. A titration test confirmed that it was a HTLA antibody. He was given one least-incompatible unit of RBC without any adverse events, and his hemoglobin level increased to 9.3 g/dL. The patient’s sample was referred to a reference laboratory and the antibody was identified as anti-JMH. He was successfully transfused with 6 additional units of least-incompatible RBCs without complication. HTLA antibodies against high frequency antigens, such as anti-JMH, are less likely to cause significant destruction of transfused antigen positive RBCs. However, identifying the specificity of these antibodies is necessary to appropriately understanding the clinical significance of the antibody, detecting other clinically important alloantibodies that may coexist, and determining the appropriate blood for transfusion.

항-K 항체가 검출된 세 예와 한국인에서의 KEL 유전자 빈도

장호은,이경,홍윤지,김택수,송상훈,박경운,송정한,한규섭 대한수혈학회 2011 大韓輸血學會誌 Vol.22 No.1

Background: Anti-K is one of the most significant unexpected antibodies that cause hemolytic transfusion reactions. Individuals with anti-K have to be transfused with K antigen-negative red cells. Although Koreans rarely have the K antigen, we have detected three cases of anti-K and we analyzed the Kell blood group genotypes for the KEL*1/KEL*2 alleles at the same time. Methods: We analyzed the KEL*1/KEL*2 allele genotypes from 261 blood donors at Seoul National University Bundang Hospital. Kell genotyping were carried out using polymerase chain reaction (PCR) and restriction enzyme length polymorphism (RFLP). Identification of anti-K was performed using three kinds of methods;37oC albumin, an anti-human globulin phase tube, a bead cassette and a gel card. Three cases of anti-K also underwent PCR with a sequence specific primer (SSP) for Kell genotyping. For comparison, the KEL*1 allele (698C>T) was synthesized by site-directed-mutagenesis. Results: All 261 donors were KEL*2/KEL*2 homozygotes and a digested KEL*1 allele was not found. The three patients with anti-K were also KEL*2/KEL*2 homozygotes and the reactivities of the anti-K identification test were the same. Conclusion: The KEL gene frequency for the KEL*1/KEL*2 allele corresponded with that of the Kell phenotype,as was previously reported. We experienced three cases of anti-K and two out of the three were assumed that they had been transfused with the K antigen-positive blood of foreigners. This study revealed that the possibility of anti-K alloimmunization and hemolytic transfusion reactions cannot be excluded in Koreans.

조직은행에서의 기증자 적합성 판정

김택수,박경운,홍윤지,황상미,송정한,한규섭,한민제 대한수혈학회 2013 大韓輸血學會誌 Vol.24 No.2

Background:Tissues for transplantation can save lives or restore essential functions. According to national policies and regulations, access to suitable transplantation, as well as the level of safety, quality, efficacy of donation, and transplantation of tissues, differ significantly between countries. We reviewed a few guidelines on tissue banking from the aspect of screening tests. In addition, four-year experience with screening panels for donated bones and donors at a tertiary hospital is introduced. Methods:Seven national and international guidelines for screening tests for donors and donated tissues were reviewed. At our institution, screening tests for donation involve two steps. At retrieval, the first screening panel, including ABO/Rh typing, unexpected antibody screening, VDRL, HBsAg, anti-HBs, anti-HBc IgM, anti-HCV, anti-HIV, and microbiological cultures was performed. The second screening panel, including the same tests, except culture studies, was performed after 90 days. From 2008 to 2011, a total of 245 retrievals of bone tissue were performed and the screening panel results were analyzed. Results:Mandatory screening serologic tests for living donors can differ according to local law or regulation and/or screening for endemic diseases. At our institution, among 245 donated bones for a period of four years, 61 bone tissues were discarded due to noncompliance for the second screening (n=32), contamination or no culture study results (n=9), abnormal serologic test results (n=8), and so on. Conclusion:Donor screening policies for tissue banking are various according to national laws or endemic disease status. Second screening tests with consideration of the window period should be adopted. 배경: 이식용 조직은 생명을 구하거나 장기의필수적인 기능을 유지시켜 준다. 국가 정책 및 규정에 따라 국가간 조직 기증 및 이식의 안전 수준, 품질, 효능 등에 큰 차이가 있다. 선별 검사의측면에서 몇 개의 조직 은행 관련 지침을 검토하였고, 이에 추가하여 4년간에 걸쳐 3차 의료기관에 기증된 뼈 조직 및 기증자를 대상으로 한 혈액선별검사 경험을 제시하였다. 방법: 7개의 국내 및 국제 가이드라인을 비교하였다. 연구가 수행된 조직은행의 선별 검사에는 두 단계가 있는데, 1차 선별검사로는 ABO/Rh, 비예기 항체 선별, VDRL, HBsAg, anti-HBs, anti- HBc IgM, anti-HCV, anti-HIV 및 미생물 배양검사를 수행하였다. 2차 선별검사는 배양검사를 제외한 나머지 항목에 대해 90일 이후에 시행하였다. 2008년부터 2011년까지, 245건의 뼈 조직이기증되었으며 이에 대한 기증자 선별검사 결과를분석하였다. 결과: 생존 기증자에 대한 필수 혈액 검사 항목은 가이드라인 별로 차이를 보였고, 국내 가이드라인 간에도 차이가 존재하였다. 4년 동안 기증된 245건의 뼈 조직 중 61건이 폐기되었는데, 2차선별검사 결과가 없는 경우(n=32), 조직 오염 또는 배양 검사 미시행한 경우(n=10), 비정상적인혈액 검사 결과(n=8) 등으로 나타났다. 결론: 국내 조직 은행에 대한 기증자 선별 정책에 있어 기준이 제시되어야 할 것으로 보이며, 이런 기준에는 2차 선별검사 및 항체미형성기간을충분히 고려할 필요가 있겠다.