랫드에서 향부자 추출물의 13주 반복 경구투여 독성평가

한소리,한형윤,박희진,민병선,정문구,문경식,정자영,노항식,석지현,김상겸 대한약학회 2013 약학회지 Vol.57 No.4

Herbal medicine has been traditionally used in Asian countries for a long time. Many pharmacological effects are identified in the herbs and these herbs are believed to be safe for human. However, the safety or adverse effect of some traditional herbal medicines has not been established. We have chosen Cyperi rhizoma based on the Korea Herbal Phar- macopoeia and which have been widely used for an anti-inflammatory effect in Korea. The object of the study was to eval- uate safety of Cyperi rhizoma in rats. The aqueous extract of Cyperi rhizoma was prepared according to the standard hot water extraction method of the Korea Pharmacopoeia. In the sub-chronic study, the aqueous extract of Cyperi rhizoma was orally administered once daily as 0, 125, 250, 500, 1000 and 2000 mg/kg/day to male and female F344 rats for 13 weeks. There were no treatment related abnormalities in mortality, clinical signs, food consumption, ophthalmologic examination, hematology, serum chemistry, urinalysis, gross observation, organ weight and histopathologic examination. In conclusion, The NOAEL (No Observed Adverse Effect Level) for Cyperi rhizoma aqueous extract was determined as more than 2000 mg/kg/day in the present experimental condition.

Evaluation of Genotoxicity and 28-day Oral Dose Toxicity on Freeze-dried Powder of Tenebrio molitor Larvae (Yellow Mealworm)

한소리,문경식,윤은영,김지영,황재삼,정은주 한국독성학회 2014 Toxicological Research Vol.30 No.2

The larval form of Tenebrio molitor (T. molitor) has been eaten in many countries and provides benefits as a new food source of protein for humans. However, no information exists regarding its safety for humans. The objective of the present study was to evaluate the genotoxicity and repeated dose oral toxicity of the freeze-dried powder of T. molitor larvae. The genotoxic potential was evaluated by a standard battery testing: bacterial reverse mutation test, in vitro chromosome aberration test, and in vivo micronucleus test. To assess the repeated dose toxicity, the powder was administered once daily by oral gavage to Sprague-Dawley (SD) rats at dose levels of 0, 300, 1000 and 3000 mg/kg/day for 28 days. The parameters which were applied to the study were mortality, clinical signs, body and organ weights, food consumption, ophthalmology, urinalysis, hematology, serum chemistry, gross findings and histopathologic examination. The freezedried powder of T. molitor larvae was not mutagenic or clastogenic based on results of in vitro and in vivo genotoxicity assays. Furthermore, no treatment-related changes or findings were observed in any parameters in rats after 28 days oral administration. In conclusion, the freeze-dried powder of T. molitor larvae was considered to be non-genotoxic and the NOAEL (No Observed Adverse Effect Level) was determined to be 3000 mg/kg/day in both sexes of SD rats under our experimental conditions.

[가작] Walk, Play, Communicate at Schoolscape

한소리 한국교육시설학회 2012 교육시설디자인 공모전 Vol.2012 No.1

Factors Affecting Suicide Attempters Visiting Emergency Departments: Through a Focus on Acquired Capability with Rehearsal for Suicide, Negative Urgency, and Social Support

한소리,이영숙,김지선,김은지,허인수,이주영,윤희정,심세훈 대한우울조울병학회 2020 우울조울병 Vol.18 No.2

This study examines how acquired capability for suicide, negative urgency, and social support effectuate a suicide attempt. Sixty-three adults having visited an emergency room due to a suicide attempt were studied. The results can be summarized as follows. First, there were no significant sex differences in acquired capability for suicide, negative urgency, and social support. Second, the correlation among suicidal ideation, acquired capability for suicide, negative urgency, social support, and suicide attempts was examined. Results showed that suicide attempts were significantly and positively correlated with suicide ideation, acquired capability for suicide, and negative urgency. However, social support and suicide attempts did not significantly correlate. Third, results of hierarchical regression analysis indicated that acquired capability for suicide and negative urgency significantly predicted suicide attempts even when suicide ideation was controlled, but social support did not significantly explain attempts. In other words, study results imply that suicide attempts increase when acquired capability for suicide and negative urgency are high. Study results offer empirical data for understanding the intrinsic characteristics of individuals attempting suicide.

쉼터거주여성의 분노표현 양식과 인생태도 변화를 위한 집단미술치료 사례연구

한소리,신현복 사)한국아동발달지원연구소 2023 임상미술심리연구 Vol.13 No.2

본 연구는 쉼터거주여성의 분노표현 양식과 인생태도 변화를 위한 집단미술치료 사례연구이다. 연구대상은 A시에 있는 쉼터거주여성 5명이며, 연구기간은 2022년 6월부터 2022년 8월까지 주 2회, 회기별 120분씩 총 12회기로 집단미술치료 프로그램은 구조화 집단형식으로 실시하였다. 연구자료 수집 및 분석 방법은 집단미술치료 프로그램 사전과 사후에 분노표현 양식(AX-Anger Expression Scale)척도와 인생태도 척도를 실시하여 윌콕슨 부호-순위 검증(Wilcoxon Signed Ranks Test)으로 분석하였으며, 집단미술치료 프로그램 회기별 진행 과정을 질적 분석하였다. 연구결과는 다음과 같다. 첫째, 집단미술치료 단계별 진행과정에서 쉼터거주여성의 집단참여, 작품 내용, 언어적 표현, 행동 관찰에 긍정적인 변화가 나타났다. 둘째, 쉼터거주여성의 분노표현 양식의 하위요인 중 분노조절에서 유의미한 변화를 보였다. 셋째, 인생태도의 하위요인 중 자기긍정과 자기부정에서 유의미한 변화가 나타났다. 본 연구는 쉼터거주여성에게 집단미술치료를 적용하여 분노표현 양식과 인생태도에 미치는 영향을 확인하고 임상현장에 기초자료를 제공하는 데 그 의의가 있다.

유제품 및 가공식품에서 Listeria monocytogenes 검출을 위한 배지법과 신속 검사키트의 유효성 검증

한소리,현지연,김희연,박종석,허석,신호철,서건호,Han, So-Ri,Hyeon, Ji-Yeon,Kim, Hee-Yun,Park, Jong-Seok,Heo, Seok,Shin, Ho-Chul,Seo, Kun-Ho 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.5

Listeria monocytogenes is a foodborne pathogen inducing listeriosis in human. We compared two different culture methods for detection of L. monocytogenes and validated two commercial kits, $VIDAS^{(R)}$ and $REVEAL^{(R)}$ for Listeria. L. monocytogenes was inoculated into various food samples to generate partial positive samples. The inoculated samples were enriched in half-Fraser broth for 48 hr at $30^{\circ}C$. The enriched samples were streaked onto Oxford agar at 24 and 48 hr postincubation followed by biochemical confirmation and concurrently analyzed by using the two commercial kits for comparison. When the enrichment period was extended from 24 to 48 hr, the numbers of positive samples were dramatically increased from 6 to 52 out of 80 samples tested using the culture method. With the commercial kits, the numbers of positive samples were also significantly increased from 10 to 18 and 1 to 18, respectively, when the enrichment period was extended from 48 to 72 hr. There was no statistical difference between the 24 hr culture method and $VIDAS^{(R)}$ or $Reveal^{(R)}$ with 48 hr enrichment. In conclusion, the 24 hr for the culture method was insufficient to detect L. monocytogenes in various foods. The commercial kits could be adequate means for presumptive screening of L. monocytogenes in food.

여성 독거노인의 행복감 증진을 위한 집단미술치료 사례연구

한소리,김현정 사)한국아동발달지원연구소 2022 임상미술심리연구 Vol.12 No.3

This study is a case study of group art therapy that examines the change in happiness of the elderly women living alone. The subjects of the study were four elderly women in their eighty who use the care center for the elderly living alone, and lived alone for more than a year. The group type of the group art therapy program is a subject-centered structural homogeneous group, and the study period was conducted once a week with a total of eight sessions, 80 minutes per session The method of collecting and analyzing the research data was analyzed with the Wilcoxon Signed Ranks Test by conducting a pre- and post happiness scale of the group art therapy program. And the changes in language expression, emotion, and behavior of the study subjects were qualitatively analyzed by stage of the group art therapy program. The results of this study showed no significant difference in group art therapy on the scale, but showed a positive change in the improvement of the happiness an elderly woman living alone in the process of each session. Group art therapy helped emotional relaxation and psychological stability through the medium, and group members experienced a sense of achievement and satisfaction on their own with the completed work. In addition, positive emotional communication with new people and interpersonal relationships gave off lively and creative energy, and showed a change in attitudes accompanied by active behavior. This study is meaningful in confirming the effect of group art therapy on changes in happiness and providing basic data to clinical sites by applying group art therapy to the elderly woman living alone.

Evaluation of Genotoxicity and 28-day Oral Dose Toxicity on Freeze-dried Powder of Tenebrio molitor Larvae (Yellow Mealworm)

한소리,윤은영,김지영,황재삼,정은주,문경식 한국독성학회 2016 Toxicological Research Vol.32 No.2

The larval form of Tenebrio molitor (T. molitor) has been eaten in many countries and provides benefits as a new food source of protein for humans. However, no information exists regarding its safety for humans. The objective of the present study was to evaluate the genotoxicity and repeated dose oral toxicity of the freeze-dried powder of T. molitor larvae. The genotoxic potential was evaluated by a standard battery testing: bacterial reverse mutation test, in vitro chromosome aberration test, and in vivo micronucleus test. To assess the repeated dose toxicity, the powder was administered once daily by oral gavage to Sprague-Dawley (SD) rats at dose levels of 0, 300, 1000 and 3000 mg/kg/day for 28 days. The parameters which were applied to the study were mortality, clinical signs, body and organ weights, food consumption, ophthalmology, urinalysis, hematology, serum chemistry, gross findings and histopathologic examination. The freezedried powder of T. molitor larvae was not mutagenic or clastogenic based on results of in vitro and in vivo genotoxicity assays. Furthermore, no treatment-related changes or findings were observed in any parameters in rats after 28 days oral administration. In conclusion, the freeze-dried powder of T. molitor larvae was considered to be non-genotoxic and the NOAEL (No Observed Adverse Effect Level) was determined to be 3000 mg/kg/day in both sexes of SD rats under our experimental conditions.

미용학과 헤어 전공자의 전공만족도가 직무만족도에 미치는 영향

한소리 ( So-ri Han ),채수기 ( Su-gi Chae ),김민 ( Min Kim ) 한국미용예술경영학회 2014 미용예술경영연구 Vol.8 No.3

We aimed to investigate the effects on job satisfaction have major impacts satisfaction of Dept. of Beauty Arts Hair majors study. Targets who major in Dept. of Beauty Arts Hair were set working in Seoul and Gyeonggi-do area. Ninety-nine data which collected through questionnaire was analyzed by SPSS 20.0 and applied analytical methods were factor analysis, reliability analysis, frequency analysis, multiple regression analysis. The research results showed that the influence on job satisfaction of Dept. of Beauty Arts Hair majors' major satisfaction has significant influence. Among the job satisfaction factor, all factors affected major satisfaction except salary satisfaction factor. It can be seen that, I'm sure and satisfy on my major, regarding my job as important, enjoying task, thinking as getting recognition in workplace and satisfying on position, working environment, service environment. It was found that Dept. of Beauty Arts Hair major's major satisfaction had affect on job satisfaction. We can see the importance of choosing major that satisfaction in workplace grows as major choice satisfying. As salary and major satisfaction hasn't significant influence, it seems to be that people do not choose major having salary in mind. Therefore, based on the results of studies, if you are trying to create an fostering environment in which guaranteed future system rather than salary and in workplace, it will be able to help encourage employee's work satisfaction.

Tracking the Fate of Muscle-derived Stem Cells: an Insight into the Distribution and Mode of Action

박형섭,최금희,한소리,유영선,정인목,이태승 대한혈관외과학회 2014 Vascular Specialist International Vol.30 No.1

Purpose: To examine the fate of muscle-derived stem cells (MDSC) after injection into different host conditions and provide an insight for their mechanism of action. Materials and Methods: MDSCs differentiated in vitro towards the endothelial lineage and transfected with lentivirus tagged with green fluorescent protein (GFP) were injected into two animal models mimicking vascular diseases: hindlimb ischemia and carotid injury models. Injected cells were tracked at the site of injection and in remote organs by harvesting the respective tissues at different time intervals and performing immunofluorescent histological analyses. Stem cell survival was quantified at the site of injection for up to 4 weeks. Results: MDSCs were successfully tagged with fluorescent material GFP and showed successful implantation into the respective injection sites. These cells showed a higher affinity to implant in blood vessel walls as shown by double fluorescent co-stain with CD31. Quantification of stem cell survival showed a timede pendent decrease from day 3 to 4 weeks (survival rate normalized against day 3 was 72.0% at 1 week, 26.8% at 2 weeks and 2.4% at 4 weeks). Stem cells were also fo und in distant organs, especially the kidneys and liver, which survived up to 4 weeks. Conclusion: MDSCs were successfully tracked in different vascular disease models, and their fate was assessed in terms of cell survival and distribution. Better understanding of the donor cell properties, including their interaction with the host conditions and their mechanism of action, are needed to enhance cell survival and achieve improved outcomes.