http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
택사(Alismatis Rhizoma) 추출물의 반복 경구투여 독성 연구
노항식,석지현,정자영,이종권,김태성,최혜경,하헌용,Roh, Hang-Sik,Seok, Ji-Hyun,Jeong, Ja-Young,Lee, Jong-Kwon,Kim, Tae-Sung,Choi, Hye-Kyung,Ha, Hun-Yong 대한한방안이비인후피부과학회 2014 한방안이비인후피부과학회지 Vol.27 No.1
Objectives : This study was carried out to evaluate the repeated dose oral toxicity of Alismatis Rhizoma in Sprague-Dawley(SD) rats. Methods : Male and female rats were administered orally with Alismatis Rhizoma water extract of 500 mg/kg(low dosage group), 1,000 mg/kg(middle dosage group) and 2,000 mg/kg(high dosage group). We daily observed number of deaths, clinical signs and gross findings for 14 days(twice a day). After 14 days, we measured body and organs weight. Also we analyzed hematological changes. Results : No dead SD rats and no clinical signs were found during the experiment period. Also other specific changes were not found between control and treated groups in hematology and serum biochemistry. In addition no significant changes of gross body and individual organs weight. Conclusions : These results suggest that water soluble extract of Alismatis Rhizoma has not repeated dose oral toxicity and oral LD50 value was over 2,000 mg/kg in SD rats. As a result, we can determine Alismatis Rhizoma is a relatively safe substance.
원지(Root of Polygala teunifolia Willd)추출물의 급성 경구투여 독성 연구
노항식 ( Hang Sik Roh ),정자영 ( Ja Young Jeong ),석지현 ( Ji Hyun Seok ),하헌용 ( Hun Yong Ha ) 대한한방부인과학회 2013 大韓韓方婦人科學會誌 Vol.26 No.4
In this study, it was carried out to evaluate the acute oral toxicity of Root of Polygala teunifolia Willd. in Sprague-Dawley (SD) rats. Methods: Male and female rats were administered orally with Root of Polygala teunifolia Willd. water extract of 1,000 mg/kg (low dosage group), 2,000 mg/kg (middle dosage group) and 4,000 mg/kg (high dosage group). We daily observed number of deaths, clinical signs and gross findings for 7 days. After 7 days, we measured body and organs weight. Also we analyzed hematological changes. Results: No dead SD rats and no clinical signs were found during the experiment period. Also other specific changes were not found between control and treated groups in hematology and serum biochemistry. But we found out histopathological changes in liver fat tissues of female. In addition, there were no significant changes of gross body and individual organs weight. Conclusions: These results suggest that water soluble extract of Root of Polygala teunifolia Willd. has not acute oral toxicity and oral LD50 value was over 4,000 mg/kg in SD rats.
택사(Alismatis Rhizoma) 추출물의 급성 경구투여 독성 연구
석지현,노항식,정자영,하헌용,Seok, Ji-Hyun,Roh, Hang-Sik,Jeong, Ja-Young,Ha, Hun-Yong 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.4
Objectives : This study was carried out to evaluate the acute oral toxicity of Alismatis Rhizoma in Sprague-Dawley(SD) rats. Methods : male and female rats were administered orally with Alismatis Rhizoma water extract of 1,000 mg/kg (low dosage group), 2,000 mg/kg(middle dosage group) and 4,000 mg/kg(high dosage group). We daily observed number of deaths, clinical signs and gross findings for 7 days. After 7 days, we measured body and organs weight. Also we analyzed hematological changes. Results : No dead SD rats and no clinical signs were found during the experiment period. Also other specific changes were not found between control and treated groups in hematology and serum biochemistry. In addition no significant changes of gross body and individual organs weight. Conclusions : These results suggest that water soluble extract of Alismatis Rhizoma has not acute oral toxicity and oral $LD_{50}$ value was over 4,000 mg/kg in SD rats.
Evaluation of Haemagglutinin Content by RP-HPLC to Generate Pandemic Influenza Vaccine
강현경,노항식,송혜민,이광문,정승태,반상자,모인필,안범수,안치영 한국독성학회 2016 Toxicological Research Vol.32 No.4
The potency of influenza vaccine is determined based on its hemagglutinin (HA) content. In general, single radial immunodiffusion (SRID) assay has been utilized as the standard method to measure HA content. However, preparation of reagents for SRID such as antigen and antibody takes approximately 2~3 months, which causes delays in the development of influenza vaccine. Therefore, quantification of HA content by other alternative methods is required. In this study, we measured HA contents of H1N1 antigen and H1N1 influenza vaccine by reverse phase-high performance liquid chromatography (RP-HPLC) methods. The presence of HA1 and HA2 was investigated by silver staining and Western blot assay. In addition, accuracy and repeatability of HA measurement by RP-HPLC were evaluated. Comparison of HA concentration by SRID and RP-HPLC revealed a precise correlation between the two methods. Our results suggest that RP-HPLC assay can replace SRID in the event of a pandemic flu outbreak for rapid vaccine development.
Atractylodes macrocepala KOIDZ.(백출) 추출물의 급성 경구투여 독성 연구
최혜경,노항식,정자영,하헌용 한국자원식물학회 2014 한국자원식물학회지 Vol.27 No.1
Atractylodes macrocepala KOIDZ. (AmK) is a herbal medicine and resources of functional food which has been used for the treatment of indigestion, anorexia, diarrhea and digestive dysfunction. Recently AmK is frequently used as resources of functional food and whitening cosmetics. In this study was carried out to evaluate the acute oral toxicity of Amk in Sprague-Dawley(SD) rats. male and female rats were administered orally with Amk extract of 1,000 ㎎/㎏ (low dosage group), 2,000 ㎎/㎏ (middle dosage group) and 4,000 ㎎/㎏ (high dosage group). We daily observed number of deaths, clinical signs and gross findings for 7 days. No dead SD rats and no clinical signs were found during the experiment period. Also other specific changes were not found between control and treated groups in hematology and serum biochemistry. But we found out feeble histopathological changes in liver fat tissues. In addition no significant changes of gross bady and individual organs weight. These results suggest that water soluble extract of AmK has not acute oral toxicity and oral LD 50 value was over 4,000 /㎎㎏ in SD rats. 본 연구는 Atractylodes macrocepala KOIDZ. 의 급성 경구독성을 평가하기 위하여 SD 계 Rat 를 이용하여 농도별 열수 추출물을 투여하고 체중변화 , 이상반응 , 장기무게변화 , 혈액학적이상반응 , 조직병리학적 이상반응 등을 측정하였다 . Atractylodes macrocepala KOIDZ. 를 투여한 실험군에서 비교적 낮은 농도에서 체중의 감소가 관찰되었으나 , 이상반응이나 장기무게에있어 유의성 있는 변화는 관찰되지 않았다 . 혈액학적 지표에 있어서도 비교적 낮은 농도에서 WBC 및 AST 의 증가가 관찰되었다 . 조직병리학적 소견에서 일부 간조직의 지방변성이 관찰되어 간독성과 간세포에 미치는 영향에 대한 연구가 필요할 것으로 판단된다 . 이와 같은 연구의 결과를 종합해 볼 때 백출이 경구투여에 있어 비교적 안전한 물질인 것으로 판단할 수 있다 . 그러나 단회 경구투여 급성독성시험만으로 천연물 생약에 대한독성 유무를 판단하기에는 일정부분 제약이 있으므로 , 추가적으로 2주 (또는 4주) 반복 경구투여 독성시험 및 13 주 반복 경구투여 독성시험 그리고 유전독성에 대한 연구들이 순차적으로수행되어야 하며 , 이를 통하여 백출에 대한 체계적인 독성정보를 구축함으로써 보다 정확하고 과학적 근거에 입각한 안전성자료가 확보될 수 있을 것으로 기대한다.
백선피(Dictamnus dasycarpus Turcz) 추출물의 급성 경구투여 독성 연구
석지현,노항식,정자영,하헌용,Seok, Ji-Hyun,Roh, Hang-Sik,Jeong, Ja-Young,Ha, Hun-Yong 대한한방안이비인후피부과학회 2014 한방안이비인후피부과학회지 Vol.27 No.1
Objectives : This study was carried out to evaluate the acute oral toxicity of Dictamnus dasycarpus Turcz in Sprague-Dawley(SD) rats. Methods : Male and female rats were administered orally with Dictamnus dasycarpus Turcz water extract of 1,000 mg/kg(low dosage group), 2,000 mg/kg(middle dosage group) and 4,000 mg/kg(high dosage group). We daily observed number of deaths, clinical signs and gross findings for 7 days. After 7 days, we measured body and organs weight. Also we analyzed hematological changes. Results : No dead SD rats and no clinical signs were found during the experiment period. Also other specific changes were not found between control and treated groups in hematology. But we found out subtle changes in body weight and individual organ weight of the female group. In addition specific changes were observed in serum biochemical value of female group. Conclusions : These results suggest that water soluble extract of Dictamnus dasycarpus Turcz has not acute oral toxicity and oral $LD_{50}$ value was over 4,000 mg/kg in SD rats. Also Dictamnus dasycarpus Turcz is expected to be sensitive with respect to the female.
김영희,곽경아,김태성,석지현,노항식,이종권,정자영,맹은호,홍정섭,이윤석,강진석 한국독성학회 2015 Toxicological Research Vol.31 No.2
Nanotechnology has advanced at an extremely rapid pace over the past several years in numerous fields of research. However, the uptake of nanoparticles (NPs) into the body after administration through various routes may pose a risk to human health. In this study, we investigated the potential ocular toxicity of 20- nm, negatively- charged zinc oxide (ZnO) NPs in rats using micro-computed tomography (micro-CT) and histopathological assessment. Animals were divided into four groups as control group, ZnO NPs treatment group (500 mg/kg/day), control recovery group, and ZnO NPs treatment and recovery group. Ocular samples were prepared from animals treated for 90 days (10 males and 10 females, respectively) and from recovery animals (5 males and 5 females, respectively) sacrificed at 14 days after final treatment and were compared to age-matched control animals. Micro-CT analyses represented the deposition and distribution of foreign materials in the eyes of rats treated with ZnO NPs, whereas control animals showed no such findings. X-ray fluorescence spectrometry and energy dispersive spectrometry showed the intraocular foreign materials as zinc in treated rats, whereas control animals showed no zinc signal. Histopathological examination revealed the retinopathy in the eyes of rats treated with ZnO NPs. Neuronal nuclei expression was decreased in neurons of the ganglion cell layer of animals treated with ZnO NPs compared to the control group. Taken together, treatment with 20-nm, negatively-charged ZnO NPs increased retinopathy, associated with local distribution of them in ocular lesions.
음양곽, 백출 및 원지의 독성평가를 위한 성분분석 및 안정성 시험
금정호,한형윤,석지현,노항식,이종권,정자영,김정아,우미희,최재수,민병선 한국생약학회 2014 생약학회지 Vol.45 No.2
A simple and reliable reverse phase HPLC method was developed to determine pharmacologically active markercompounds of Epimedii Herba, Atractylodis Rhizoma Alba, and Polygalae Radix. The stability test of water-extracts of thethree natural medicines has been evaluated for six months. However, no significant changes in the content of the marker compoundsof each extract were observed during the time of investigation.
랫드에서 향부자 추출물의 13주 반복 경구투여 독성평가
한소리,한형윤,박희진,민병선,정문구,문경식,정자영,노항식,석지현,김상겸 대한약학회 2013 약학회지 Vol.57 No.4
Herbal medicine has been traditionally used in Asian countries for a long time. Many pharmacological effects are identified in the herbs and these herbs are believed to be safe for human. However, the safety or adverse effect of some traditional herbal medicines has not been established. We have chosen Cyperi rhizoma based on the Korea Herbal Phar- macopoeia and which have been widely used for an anti-inflammatory effect in Korea. The object of the study was to eval- uate safety of Cyperi rhizoma in rats. The aqueous extract of Cyperi rhizoma was prepared according to the standard hot water extraction method of the Korea Pharmacopoeia. In the sub-chronic study, the aqueous extract of Cyperi rhizoma was orally administered once daily as 0, 125, 250, 500, 1000 and 2000 mg/kg/day to male and female F344 rats for 13 weeks. There were no treatment related abnormalities in mortality, clinical signs, food consumption, ophthalmologic examination, hematology, serum chemistry, urinalysis, gross observation, organ weight and histopathologic examination. In conclusion, The NOAEL (No Observed Adverse Effect Level) for Cyperi rhizoma aqueous extract was determined as more than 2000 mg/kg/day in the present experimental condition.
한형윤,김정아,한수철,양영수,김수남,이정화,정자영,노항식,석지현,민병선,한강현 한국생약학회 2015 Natural Product Sciences Vol.21 No.1
The objective of this study is to characterize a toxicity of Epimedii Herba (EH) in F344 rats and to find a dose levels for the 13 weeks toxicity study. EH is well known as medicinal herb in many Asian countries for traditional medicines of antibacterial and antiviral effects, estrogenic and antiestrogenic effects, and for treatment of osteoporosis, hypotensives, fatigue, kidney disorders, and related complications. However, the indispensable and basic information of toxicological evaluation of EH extract is insufficient to support its safe use. Therefore, we conducted toxicological evaluation of this drug in compliance with OECD and MFDS guideline in this study. The extract of EH was administered orally to F344 rats at dose levels of 0, 500, 1000, 2000, 3500, and 5000 mg/kg/day for 2 weeks. Each group was composed of 5 male and female rats. In this study, there were no treatment of EH-related adverse changes in clinical observations, mortality, body weights, food consumption, urinalysis, gross finding at necropsy, and organ weight examination. Total red blood cell count, hematocrit, mean corpuscular hemoglobin concentration, total cholesterol, and phospholipid were decreased in males and females at 5000 mg/kg/day compared to the control animals. Mean corpuscular volume and reticulocyte counts were increased in males and females at 5000 mg/kg/day compared to control animals. Therefore, we recommend that dose level of 5000 mg/kg/day is a highest treatment group in 13-week EH extract exposure study for further toxicity assessment.