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최필권,서수정,황선민,이진영 한국환경분석학회 2023 환경분석과 독성보건 Vol.26 No.3
This study focuses on quantifying the impact of various factors on nonylphenol analysis and identifying the key contributors affecting the analytical outcomes. We calculated the measurement uncertainty arising from the analysis process using GC-MS, aiming to pinpoint uncertainty sources, quantify them numerically and then calculated the overall uncertainty through uncertainty synthesis. The results revealed that factors such as calibration curve construction, internal standard injection, and reproducibility through repeated testing significantly influenced the synthetic standard uncertainty. Negligible effects were observed during processes, involving concentration and filling the standard volume post-purification, as well as during sample collection. The relative expanded uncertainty for nonylphenol concentration was within 4.6%. The uncertainty contributions from each step were as follows: sample collection, calibration curve construction, injection of internal standards, filling of the 1 mL standard volume, and reproducibility from repeated testing accounted for 0.0%, 92.7%, 4.0%, 0.1%, and 3.2% of the total uncertainty, respectively.
최필권 ( Phil-kweon Choi ),허평 ( Pyung Huh ),이경희 ( Kyong-hee Lee ),조덕희 ( Deok-hee Cho ),김창규 ( Chang-gyu Kim ),김태화 ( Tae-hwa Kim ) 한국환경분석학회 2018 환경분석과 독성보건 Vol.21 No.3
In this study, mineral springs were monitored to determine whether they are being contaminated by rainfall onto microorganisms. In addition, the sterilization efficiency of an ultraviolet sterilizer used in various mineral springs was investigated. It was observed that the incongruity rates of total coliforms, fecal coliforms, and heterotrophic plate counts were 64.8%, 20.1%, and 11.4% respectively. In July, when rainfall was high, the incongruity rates of microorganism-related characteristics were at their highest (76%), suggesting that changes in precipitation were directly related to incongruity rates of microorganism-related characteristics within mineral springs. A T-test result of 0.002 (p<0.05, significant, 2-tailed) between the clay contents of soil in the valley and those of a control soil suggested that the possibility of underground penetration of soil in the valley is higher than that of general soil. Results of the sterilization test showed that E. coli was inactivated at the time of contact with an ultraviolet intensity of 10,000±20 μW/cm2 for at least 3 seconds and no photorecovery was observed.
토양 서식 미생물을 이용한 자일렌(xylene) 분해특성 조사
최필권(Phil Kweon Choi),허평(Pyeung Heo),이상섭(Sang Seob Lee) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.6
본 연구의 목적은 유류로 오염된 토양으로부터 분리된 고효율 분해균주에 의한 xylene 분해특성을 조사하고 분해과정을 밝히는 것이다. P. putida BJ10에 의해 mineral salts medium (MSM)배지내에서 24시간 배양 후 제거율은 o, m, p-xylene각각 94, 90, 98%였으며 3% 이하의 대조군과 명확한 차이를 보였다. 또한 유류로 오염된 토양내에서의 9일 경과 후 xylene제거율은 유류 분해균주 주입구에서 66%였으며 무처리 일반토양에서 32%, 멸균 토양에서 8%로서 P. putida BJ10에 의한 유효성을 확인할 수 있었다. 또한 시간경과에 따른 대사산물을 분석한 결과 o-xylene의 분해 경로에서는 6시간 경과 후 3-methylcatechol, 24시간 경과 후 o-toluic acid 가 분해산물로서 검출되어 최종산물로서의 o-toluic acid 가 확인되었으며 중간산물의 변환과정은 기존 발표된 연구들과 다소 다른 결과를 나타내었다. The purpose of this study is to investigate the biodegradation characteristics of the xylene by BTEX-degrading bacteria, Pseudomonas putida BJ10, isolated from oil-contaminated soil and bio-degradation pathway of the xylene. The removal efficiencies of o, m, p-xylene in mineral salts medium (MSM) by P. putida BJ10 were 94, 90 and 98%, respectively for 24 hours. It shows clear difference compared with the control groups which were below 3%. The removal efficiencies of BTEX by P. putida BJ10 in gasoline- contaminated soil were 66% for 9 days. They were clearly distinguished from the control groups (control and sterilized soil) which were 32 and 8%. 3-methylcatechol and o-toluic acid were detected after 6 and 24 hours during the o-xylene biodegradation pathway. Therefore, we confirmed o-toluic acid as the final metabolite. And intermediate-products were somewhat different with previously published studies of the transformation pathway from o-xylene to 3-methylcatechol.
서수정,최필권,남우경,황선민,이진영,정선희,박서경 한국환경분석학회 2023 환경분석과 독성보건 Vol.26 No.2
This study aimed to evaluate the discharge characteristics of nonylphenol (NP) and octylphenol (OP) in the effluent of wastewater treatment facilities in the southern Gyeonggi-do. The detection rates of NP and OP were found to be 39.3% and 43.1%, respectively, with median values ranging from 1 to 3 μg/L across all industries. The industries that showed a detection rate of 50% or more were car wash, chemical experiment, fabric leather, paper wood, and plating facilities, with rates ranging from 50% to 75%, while other industries had detection rates of 21.7% to 36.8%. In terms of facility size, small-size establishments such as Type 4 and Type 5 showed higher detection rates in the "Na" region where somewhat relaxed standards are applied on a regional basis. This is because the proportion of Type 4 and Type 5 industries, including various concentration distributions of chemical experiments, research facilities, car washes, petrochemical, and plating industries, is higher than 77.7% overall and concentrated at a rate of 55.6% or higher in the “Na” region. Furthermore, the correlation coefficient (R) between NP and OP exhibited moderate association with values of 0.45 and 0.60 for car washes and plating industries, respectively. Chemical experiments, paper and wood, electrical and electronic, and medicine and hospital facilities showed strong association with correlation coefficients of 0.83, 0.82, 0.82, and 0.79, respectively. The findings in this study can be expected to serve as fundamental data for NP and OP in the establishment of water quality pollutants designation and discharge standards (draft) currently being pursued.