Role of MRI and Plain Radiograph to Diagnose Fibrous Dysplasia Mimicking Metastasis on PET/CT in a Patient with Breast Cancer

조송미,지원희,유이령,이아원,정양국,Cho, Song-Mee,Jee, Won-Hee,Yoo, Ie-Ryung,Lee, Ah-Won,Chung, Yang-Guk The Korean Musculoskeletal Tumor Society 2010 대한골관절종양학회지 Vol.16 No.1

Fibrous dysplasia is a common benign disorder of bone in which normal bone marrow is replaced with fibro-osseous tissue. As PET/CT is increasingly used for the staging of different malignant disease, incidentally found fibrous dysplasia with increased FDG uptake may mimic metastasis. We report on a 46-year-old woman with fibrous dysplasia who underwent PET/CT because of suspected recurrence of breast cancer and was misdiagnosed as a bony metastasis with a focal FDG uptake on left proximal femur. This lesion was interpreted as fibrous dysplasia based on MRI in addition to the plain radiographs. We conclude that MRI in addition to radiography may help to differentiate fibrous dysplasia mimicking metastasis on PET/CT in the patients with malignancy. 섬유형성이상은 골수가 섬유-골성 조직으로 치환되는 흔한 양성 골 질환이다. 암환자에서 병기의 결정과 추적 검사 시 암 전이의 발견에 PET/CT의 역할이 증가됨에 따라 우연히 발견된 FDG 섭취 증가를 보이는 섬유형성이상에서 암 전이와의 감별이 중요하다. PET/CT를 시행한 46세의 유방암 환자에서 좌측 대퇴골에 국소 FDG 섭취를 보여 암 전이로 의심되었으나 단순촬영과 자기공명 영상 소견에서 섬유형성이상으로 진단한 환자의 증례를 보고하고자 한다. 이 환자에서 단순활영과 자기공명영상 소견은 PET/CT에서 유방암 전이로 오인된 섬유형성이상의 감별에 도움이 되었다.

Nitric Oxide and Hydrogen Peroxide Production are Involved in Systemic Drought Tolerance Induced by 2R,3R-Butanediol in Arabidopsis thaliana

조송미,김영철,김용환,ANNE J. ANDERSON 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.4

2R,3R-Butanediol, a volatile compound produced by certain rhizobacteria, is involved in induced drought tolerance in Arabidopsis thaliana through mechanisms involving stomatal closure. In this study, we examined the involvement of nitric oxide and hydrogen peroxide in induced drought tolerance, because these are signaling agents in drought stress responses mediated by abscisic acid (ABA). Fluorescence-based assays showed that systemic nitric oxide and hydrogen peroxide production was induced by 2R,3R-butanediol and correlated with expression of genes encoding nitrate reductase and nitric oxide synthase. Co-treatment of 2R,3Rbutanediol with an inhibitor of nitrate reductase or an inhibitor of nitric oxide synthase lowered nitric oxide production and lessened induced drought tolerance. Increases in hydrogen peroxide were negated by cotreatment of 2R,3R-butanediol with inhibitors of NADPH oxidase, or peroxidase. These findings support the volatile 2R,3R-butanediol synthesized by certain rhizobacteria is an active player in induction of drought tolerance through mechanisms involving nitric oxide and hydrogen peroxide production.

Granulocytic Sarcoma in the Leg Mimicking Hemorrhagic Abscess

조송미,지원희 대한자기공명의과학회 2009 Investigative Magnetic Resonance Imaging Vol.13 No.1

Granulocytic sarcoma is a rare extramedullary tumor composed of immature granulocytic precursors. Usually, granulocytic sarcoma is seen in association with acute myeloid leukemia, or other myeloproliferative disorders. Rarely, it may manifest as a primary presentation before the onset of systemic disease in acute myeloid leukemia. The clinical suspicion of granulocytic sarcoma based on imaging findings is important for the management of the patient especially when systemic disease of acute myeloid leukemia is not confirmed as in our case. We report the MR findings of a granulocytic sarcoma in the left leg mimicking hemorrhagic abscess in a patient with acute myeloid leukemia. We believe that MRI can be a helpful diagnostic method of making a differential diagnosis of granulocytic sarcoma in a patient with leukemia, and this can be done by analyzing the signal intensity and the enhancement pattern.

Induced Systemic Drought and Salt Tolerance by Pseudomonas chlororaphis O6 Root Colonization is Mediated by ABA-independent Stomatal Closure

조송미,김영철,강범룡,김정준 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.2

Root colonization by the rhizobacterium Pseudomonas chlororaphis O6 in Arabidopsis thaliana Col-0 plants resulted in induced tolerance to drought and salinity caused by halide salt-generated ionic stress but not by osmotic stress caused by sorbitol. Stomatal apertures decreased following root colonization by P. chlororaphis O6 in both wild-type and ABA-insensitive Arabidopsis mutant plants. These results suggest that an ABAindependent stomatal closure mechanism in the guard cells of P. chlororaphis O6-colonized plants could be a key phenotype for induced systemic tolerance to drought and salt stress.

Transcriptome Analysis of Induced Systemic Drought Tolerance Elicited by Pseudomonas chlororaphis O6 in Arabidopsis thaliana

조송미,김영철,강범룡 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.2

Root colonization by Pseudomonas chlororaphis O6induces systemic drought tolerance in Arabidopsis thaliana. Microarray analysis was performed using the 22,800-gene Affymetrix GeneChips to identify differentiallyexpressed genes from plants colonized with or without P. chlororaphis O6 under drought stressed conditions or normal growth conditions. Root colonization in plants grown under regular irrigation condition increased transcript accumulation from genes associated with defense,response to reactive oxygen species, and auxin- and jasmonic acid-responsive genes, but decreased transcription factors associated with ethylene and abscisic acid signaling. The cluster of genes involved in plant disease resistance were up-regulated, but the set of drought signaling response genes were down-regulated in the P. chlororaphis O6-colonized under drought stress plants compared to those of the drought stressed plants without bacterial treatment. Transcripts of the jasmonic acid-marker genes, VSP1 and pdf-1.2, the salicylic acid regulated gene, PR-1, and the ethylene-response gene,HEL, also were up-regulated in plants colonized by P. chlororaphis O6, but differed in their responsiveness to drought stress. These data show how gene expression in plants lacking adequate water can be remarkably influenced by microbial colonization leading to plant protection,and the activation of the plant defense signal pathway induced by root colonization of P. chlororaphis O6 might be a key element for induced systemic tolerance by microbes.

Identification and Transcriptional Analysis of Priming Genes in Arabidopsis thaliana Induced by Root Colonization with Pseudomonas chlororaphis O6

조송미,박주연,한송희,Anne J. Anderson,Kwang Yeol Yang,김영철,Brian McSpadden Gardener 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.3

Root colonization of Arabidopsis thaliana with Pseudomonas chlororaphis O6 induces systemic tolerance against diverse pathogens, as well as drought and salt stresses. In this study, we demonstrated that 11 genes in the leaves were up-regulated, and 5 genes were down-regulated as the result of three- to five-days root colonization by P. chlororaphis O6. The identified priming genes were involved in cell signaling, transcription, protein synthesis,and degradation. In addition, expression of selected priming genes were induced in P. chlororaphis O6-colonized plants subjected to water withholding. Genes encoding defense proteins in signaling pathways regulated by jasmonic acid and ethylene, such as VSP1and PDF1.2, were additional genes with enhanced expression in the P. chlororaphis O6-colonized plants. This study indicated that the expression of priming genes, as well as genes involved in jasmonic acid- and ethylene-regulated genes may play an important role in the systemic induction of both abiotic and biotic stress due to root colonization by P. chlororaphis O6.

수박(Citrullus lanatus (thunb.) Matsum. & Nakai) 자엽 절편의 재분화에 미치는 생장조절물질의 영향

조송미,오상아,최용수,박상빈 한국자원식물학회 2014 한국자원식물학회지 Vol.27 No.1

In this study, we developed a high frequency watermelon regeneration system using three breeding lines (‘B02’, ‘B05’ and ‘D04’) of watermelon (Citrullus lanatus (thunb.) Matsum. & Nakai) which are differed in their fruits in shape, color of pericarp and flesh. The highest frequency of explants with callus was observed by using explants that consist of cotyledon proximal part end in all breeding lines, and the highest rate of callus induction was obtained on MS medium containing 1.0 ㎎/L BAP + 0.5 ㎎/L IAA for ‘B02’ (94%), 3.0 ㎎/L BAP for ‘B05’ (95%), 3.0 ㎎/L BAP + 0.1 ㎎/L IAA for ‘D04’ (90%). The highest shoot regeneration rates from derived callus were obtained on MS medium containing 1.0 ㎎ /L BAP + 0.5 ㎎/L IAA for ‘B05’ (94%), and then a ‘B02’ (81%) with a same culture conditions, and the lowest regeneration was obtained on MS medium containing 1.0 ㎎/L BAP for ‘D04’ (56%). Regenerated plants showed the best rates of root formation on MS containing 0.1 ㎎/L IBA for ‘B02’ (67%), 0.1 ㎎/L NAA for ‘B05’ (87%), 0.5 ㎎/L IAA for ‘D04’ (88%). The regenerated plants showed a 100% survival rate in soil condition. The tissue culture and regeneration conditions obtained from this study will be useful for regenerating plants in breeding applications, and will be a useful tool for further genetic transformation studies on watermelons. 수박에서 과형, 과피, 과육 색이 서로 다른 중소과종인3가지 계통의 재분화 조건을 확립하고자 각 계통의 유묘의 자엽절편을 배양하여 캘러스 및 신초 형성에 미치는 생장조절물질의 효과를 조사한 결과는 다음과 같다. 각 발아된 유묘 자엽의 캘러스 형성은 모든 계통에서 유묘의 자엽 부위 중 기부(proximal)의 끝부분에서 가장 높은 효율을 나타내었다. 또한 캘러스 형성에 미치는 생장조절제의 영향은 ‘B02’의 경우1.0 ㎎/L BAP와 0.5 ㎎/L IAA를 혼합한 처리구에서, ‘B05’의 경우 1.0 ㎎/L BAP단독 처리구에서, ‘D04’의 경우 3.0 ㎎/L BAP와 0.1 ㎎/L IAA를 혼합한 처리구에서 가장 높게 나타나 계통에 따라 생장조절제의 조건이 달라짐을 확인하였다. 신초 형성율은 ‘B02’와 ‘B05’의 경우 1.0 ㎎/L BAP와 0.5 ㎎/L IAA를 혼합한 처리구에서 ‘D04’의 경우 1.0 ㎎/L BAP 단독 처리구에서 가장 효과적인 것으로 확인되었다. 재분화된 식물체의 길이신장은 MS 기본 배지에 생장조절제로 1 ㎎/L BAP 가 첨가된 배지에서 가장 효과적이었으며, 이들 식물체의 최적 발근은 ‘B02’ 경우 0.5 ㎎/L IBA, ‘B05’ 경우 0.1 ㎎/L NAA, ‘D04’ 경우 0.5 ㎎/L IAA를 첨가된 배지 조건에서 이루어졌다.

A Case of a Retained Surgical Sponge after Endoscopic Sinus Surgery Depicted on CT Imaging

조송미,김병국 대한영상의학회 2009 대한영상의학회지 Vol.61 No.2

A retained surgical sponge is an uncommon complication in endoscopic sinus surgery. A 53-year-old woman who underwent endoscopic sinus surgery two years prior presented with nasal stuffiness and posterior nasal dripping that had persisted for one year. On CT images, a soft-tissue mass with mixed high and low attenuation was noted in the posterior air cells of the right ethmoid sinus. CT imaging features of the surgical sponge granuloma are described.

잎 절편의 재분화에 의한 참박 형질전환

조송미(Cho, Song-Mi),문선진(Moon, Sun-Jin),정수진(Chung, Soo-Jin),김미성(Kim, Mi-Seong),김영철(Kim, Young-Cheol),양광열(Yang, Kwang-Yeol),최용수(Choi, Yong-Soo),Sapkota, Kumar,조백호(Cho, Baik-Ho),김광상(Kim, Kwang-Sang) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.5

수박의 대목으로 사용되고 있는 참박의 재분화조건을 확립함으로서 병저항성 형질전환 식물체를 얻어 보고자 본 실험을 실시하였다. 참박의 자엽을 잘라 재분화에 미치는 식물생장조절물질의 효과를 조사하였고 오이 galactinol synthase (CsGolS1) 유전자를 참박에 형질전환하였던 결과는 다음과 같다. 참박 신초의 재분화는 7일째 된 유묘의 자엽부위 중 전반부위에서 기장 높은 효율을 나타내었고, cytokinin으로 BA나 zeatin을 단용처리하는 것보다 옥신으로 IAA를 혼용처리하는 것이 신초 분화에 더 효과적이었다. 복합스트레스 내성 유전자인 오이 CsGolS1유전자를 pBI121 binary vector에 재조합하여 아그로박테리움을 이용, 참박에 형질전환하였던 결과, 형질전환체는 kanamycin이 첨가된 배지에서 생장하였고 PCR 및 Southern blot 분석에 의해 유식물체의 20% 정도가 형질전환체임을 확인할 수 있었다. In order to develop a disease-resistant root stock for the growth of watermelon, an efficient regeneration system of the gourd(Lagenaria leucantha Duch.) inbred line GO701-2 via organogenesis was established in this experiment. Using proximal parts of cotyledon explant excised from germinated seedling in vitro, maximum adventitious shoot formation (39%) was achieved on MS medium where cytokinin (BA) and auxin (IAA) were added at a concentration of 3mg/L and 0.1mg/L, respectively. Roots of the elongated shoots were successfully formed on MS medium without adding any plant growth regulators. The cucumber CsGolS1 gene known as a resistance gene against biotic and abiotic stresses, was constructed into the binary vector pBI121 under the control of CaMV 35S promoter. When the gene was introduced into the genome of gourd by Agrobacterium-mediated transformation, putative transgenic plants were obtained with the transformation efficiency of approximately 20 percent.

유색수박의 잎절편에 의한 식물체 재분화

조송미(Song Mi Cho),오상아(Sang A Oh),최용수(Yong Soo Choi),백진수(Jin Su Paik),박상빈(Sang Bin Park) 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.5