백색증에 관여하는 Tyrosinase 유전자에 대한 연구

김광상(KIM Kwang Sang),김정중(KIM Jeong Joong),이황희(LEE Hwang Hee),김원신(KIM Won Shin),이희섭(RHEE Hee Sub),오재민(OH Jai Min),최민규(CHOI Min Kyu),박승택(PARK Seung Taeck),정연태(CHUNG Yeun Tai) 대한체질인류학회 1995 해부·생물인류학 (Anat Biol Anthropol) Vol.8 No.2

안 피부 백색증 환자는 tyrosinase의 활성을 측정함으로서 그 형(type)이 결정되어질 수 있다. Tyrosinase 유전자의 coding region은 5개의 exon으로 이루어지고 있으며 지금까지 안 피부 백색증 환자에 있어서 여러 가지 종류의 tyrosinase 유전자의 돌연변이들이 보고되고 있다. 따라서 본 연구에서는 국내 안 피부 백색증(Oculocutaneous albinism OCA) 환자와 그 가족 구성원들 및 정상인들을 대상으로 그들의 모구에서 tyrosinase 의 활성을 측정하였으며 tyrosinase 음성 안 피부 백색증으로 판명된 환자에 대해서는 tyrosinase 유전자에 대한 분석을 시행하여 다음과 같은 결과를 얻었다. 1 Tyrosinase assay 결과 백색증을 보이지 않는 환자의 가족 및 정상인들의 경우에는 tyrosinase 활성이 모두에서 일정하게 있었으며 각 개인 간에는 통계적으로 유의한 차이를 보이고 있었다. 2 백색증 환자의 경우에는 tyrosinase 활성이 전혀 없어 King 와 Summers의 분류에 의한다면 type IA(tyrosinase 음성 ) 안 피부 백색증이었다. 3 백색증 환자의 tyrosinase 유전자에 대한 분석 결과 exon V 중 337번째 bp와 338번째 bp 그리고 303 번째 bp와 354번째 bp에서 지금까지는 보고된 바가 없는 새로운 상입 돌연변이들을 확인하였다. 이상의 결과 본 연구의 대상이 안 피부 백색증 환자는 삽입과 같은 tyrosinase 유전자의 frame shift 돌연변이들로 인하여 tyrosinase의 기능이 상실되어 melanin의 생합성이 저애된 것으로 사료되어 진다. 하지만 이러한 돌연변이들과 안 피부 백색증과의 직접적인 관련성 여부는 transfection assay 등과 같은 주가적인 연구들에 의하여 확인되어져야 할 것이다.

보문 : 노화가 진행된 남,여 노인기 모발의 이,화학적 특성과 형태학적 변화

김광상 ( Kwang Sang Kim ),진병운 ( Buyng Un Jin ),김민 ( Min Kim ),장미여 ( Mi Ye Chang ),김복환 ( Bok Hwan Kim ),김기영 ( Ki Young Kim ) 대한미용과학회(구 한국두피모발미용학회) 2010 대한미용학회지 Vol.6 No.1

Hairs have a number of functions including thermoregulation, collecting sensory information, protection against environmental trauma, social communication and mimicry. The hair is altered and changed such as weakness of tensile strength, alopecia and canities by physical and environmental factors. In this study, the physicochemical properties and morphological change of in altered hairs were investigated in 24 spenders of adulthood and elderly. Thickness, tensile strength, water swelling and alkali solubility, breadth and thickenss of hair cuticle were measured and morphological change of cuticle and cortex were observed. In the result, thickness and tensile strength of hair as aging indicator decreased significantly in elderly men and women in contrast to adulthood (p<0.05), and both parameters were higher in men than in women of adulthood and elderly. Breadth and stepping of cuticle decreased in elderly men and women in contrast to adulthood. But water swelling and alkali solubility as hair damage indicators increased in elderly men but not women. More damaged cuticles and diffuse melanin granules appeared in elderly than in adulthood by SEM and TEM observation. In conclusion, aging induced to change of physicochemical properties and morphology, and decreased hair thickness in elderly men and women may be not decreased thickness of cuticles but loss of cuticle layers.

인터넷쇼핑몰의 VMD 구성요인에 대한 탐색적 연구

Kim Kwang Seok(金光爽),Jong Kuk Shin(申??),Dong Mo Koo(具??) 한국마케팅과학회 2008 마케팅과학연구 Vol.18 No.2

본 연구는 인터넷쇼핑몰 비주얼 머천다이징의 주요차원을 고객이 쇼핑몰에 진입한 후 정보탐색과 대안평가를 거치는 등의 쇼핑과정을 토대로 AIDA모형 관점에서 점포, 제품, 촉진에 초점을 맞추었다. VMD의 주요차원(primary dimensions)으로는 점포디자인, 머천다이징, 그리고 머천다이징단서로 구분하였다. 선행연구 결과를 토대로 점포다자인의 하위차원으로는 차별성, 간결성, 위치확인성을, 머천다이즈의 하위차원으로는 제품구색, 명성, 정보성을, 그리고 머천다이징단서의 하위차원으로는 제품추천 및 링크를 설정하여 VMD태도와의 관계를 탐색적으로 조사하였다. 연구결과 이들 세 차원은 종속변수에 유의한 정의 영항을 미치는 것으로 나타났다. This study is to empirically examine the primary dimensions of visual merchandising (VMD) of internet shopping mall, namely store design, merchandise, and merchandising cues, to be a attractive virtual store to the shoppers. The authors reviewed the literature related to the major components of VMD from the perspective of the AIDA model, which has been mainly applied to the offline store settings. The major purposes of the study are as follows; first, tries to derive the variables related with the components of visual merchandising through reviewing the existing literatures, establish the hypotheses, and test it empirically. Second, examines the relationships between the components of VMD and the attitude toward the VMD, however, putting more emphasis on finding out the component structure of the VMD. VMD needs to be examined with the perspective that an online shopping mall is a virtual self-service or clerkless store, which could reduce the number of employees, help the shoppers search, evaluate and purchase for themselves, and to be explored in terms of the in-store persuasion processes of customers. This study reviewed the literatures related to store design, merchandise, and merchandising cues which might be relevant to the store, product, and promotion respectively. VMD is a total communication tool, and AIDA model could explain the in-store consumer behavior of online shopping. Store design has to do with triggering a consumer attention to the online mall, merchandise with a product related interest, and merchandising cues with promotions such as recommendation and links that induce the desire to pruchase. These three steps might be seen as the processes for purchase actions. The theoretical rationale for the relationship between VMD and AIDA could be found in Tyagi(2005) that the three steps of consumer-oriented merchandising are a store, a product assortment, and placement, in Omar(1999) that three types of interior display are a architectural design display, commodity display, and point-of-sales(POS) display, and in Davies and Ward (2005) that the retail store interior image is related to an atmosphere, merchandise, and in-store promotion. Lee et al(2000) suggested as the web merchandising components a merchandising cues, a shopping metaphor which is an assistant tool for search, a store design, a layout(web design), and a product assortment. The store design which includes differentiation, simplicity and navigation is supposed to be related to the attention to the virtual store. Second, the merchandise dimensions comprising product assortments, visual information and product reputation have to do with the interest in the product offerings. Finally, the merchandising cues that refer to merchandiser(MD)""s recommendation of products and providing the hyperlinks to relevant goods for the shopper is concerned with attempt to induce the desire to purchase. The questionnaire survey was carried out to collect the data about the consumers who would shop at internet shopping malls frequently. To select the subject malls, the mall ranking data announced by a mall rating agency was used to differentiate the most popular and least popular five mall each. The subjects was instructed to answer the questions after navigating the designated mall for five minutes. The 300 questionnaire was distributed to the consumers, 166 samples were used in the final analysis. The empirical testing focused on identifying and confirming the dimensionality of VMD and its subdimensions using a structural equation modeling method. The confirmatory factor analysis for the endogeneous and exogeneous variables was carried out in four parts. The second-order factor analysis was done for a store design, a merchandise, and a merchandising cues, and first-order confirmatory factor analysis for the attitude toward the VMD. &nb

보문 : 생리활성물질을 이용한 여드름 피부개선에 따른 멜라닌, 홍반, pH값의 변화도

오수정 ( Su Jeong Oh ),김광상 ( Kwang Sang Kim ),김기영 ( Ki Young Kim ),김경례 ( Kyeong Rye Kim ),김경란 ( Kyeong Ran Kim ) 대한미용과학회(구 한국두피모발미용학회) 2010 대한미용학회지 Vol.6 No.3

The purpose of this research is to develop the materials for natural cosmetics that it is possible to use for the improvement of acne skin. For this, this research manufactured the skin lotion and pack including ethanol extract of Caesalpinia sappan which is a bioactive chemical and applied it to the female with acne skin. And then it tried to verify the effect of the lotion and pack by measuring melanin, erythema and the changes of pH value. The objects of this research were 16 women with acne skin in 20`s above KAGS Level II, and the changes of melanin, erythema and pH value on 3 sites of the cheek, forehead and chin on their faces were measured 4 times before and after applying the pack including 15% ethanol extract of Caesalpinia sappan to 8 women (Group A) and the skin lotion including 3% ethanol extract to 8 women (Group B), respectively. The experimental data was analyzed by repeated measures using SPSS version 12.0 program. The experimental results for the changes of melanin, erythema and pH value in accordance with improvement of acne vulgaris after applying the skin lotion and pack including the ethanol extract of Caesalpinia sappan were as followings. As a result of a human closed patch test about the ethanol extract of C. sappan in a stability test, erythema (-), allergy (-), edema (-), blister (-) and so on were not observed. So, it may deem that the skin lotion and pack are stable when applying them to a human body as cosmetics. Both the skin lotion and the pack including ethanol extract of C. sappan had a positive effect on melanin and pH value. Even though there was no large difference between two experimental groups, it showed that the skin lotion influences on erythema larger than the pack. In conclusion, the ethanol extract of Caesalpinia sappan is very meaningful for improvement of acne skin and it is expected that it will become good resource for developing functional cosmetics in future.

Hair Growth Effect of Ethanol Extract from Houttuynia cordata Thunb. Whole Plant and Calendula officinalis L. Flower

Seong Woo Jin(진성우),Kyung Je Kim(김경제),Young Woo Koh(고영우),Seung Bin Im(임승빈),Sang Wook Jeong(정상욱),Kwang Sang Kim(김광상),Min Sook Kim(김민숙),Byung Jo Yu(유병조),Ji Yeon Jang(장지연),Kyungsun Seo(서경순) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.1

잎 절편의 재분화에 의한 참박 형질전환

조송미(Cho, Song-Mi),문선진(Moon, Sun-Jin),정수진(Chung, Soo-Jin),김미성(Kim, Mi-Seong),김영철(Kim, Young-Cheol),양광열(Yang, Kwang-Yeol),최용수(Choi, Yong-Soo),Sapkota, Kumar,조백호(Cho, Baik-Ho),김광상(Kim, Kwang-Sang) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.5

수박의 대목으로 사용되고 있는 참박의 재분화조건을 확립함으로서 병저항성 형질전환 식물체를 얻어 보고자 본 실험을 실시하였다. 참박의 자엽을 잘라 재분화에 미치는 식물생장조절물질의 효과를 조사하였고 오이 galactinol synthase (CsGolS1) 유전자를 참박에 형질전환하였던 결과는 다음과 같다. 참박 신초의 재분화는 7일째 된 유묘의 자엽부위 중 전반부위에서 기장 높은 효율을 나타내었고, cytokinin으로 BA나 zeatin을 단용처리하는 것보다 옥신으로 IAA를 혼용처리하는 것이 신초 분화에 더 효과적이었다. 복합스트레스 내성 유전자인 오이 CsGolS1유전자를 pBI121 binary vector에 재조합하여 아그로박테리움을 이용, 참박에 형질전환하였던 결과, 형질전환체는 kanamycin이 첨가된 배지에서 생장하였고 PCR 및 Southern blot 분석에 의해 유식물체의 20% 정도가 형질전환체임을 확인할 수 있었다. In order to develop a disease-resistant root stock for the growth of watermelon, an efficient regeneration system of the gourd(Lagenaria leucantha Duch.) inbred line GO701-2 via organogenesis was established in this experiment. Using proximal parts of cotyledon explant excised from germinated seedling in vitro, maximum adventitious shoot formation (39%) was achieved on MS medium where cytokinin (BA) and auxin (IAA) were added at a concentration of 3mg/L and 0.1mg/L, respectively. Roots of the elongated shoots were successfully formed on MS medium without adding any plant growth regulators. The cucumber CsGolS1 gene known as a resistance gene against biotic and abiotic stresses, was constructed into the binary vector pBI121 under the control of CaMV 35S promoter. When the gene was introduced into the genome of gourd by Agrobacterium-mediated transformation, putative transgenic plants were obtained with the transformation efficiency of approximately 20 percent.

보문 : 자초의 천연색을 이용한 립밤에 관한 연구

박보름 ( Bo Reum Park ),김광상 ( Kwang Sang Kim ),박해련 ( Hae Ryeun Park ),신규옥 ( Kyu Ok Shin ),안경민 ( Kyung Min Ahn ),김기영 ( Ki Young Kim ) 대한미용과학회(구 한국두피모발미용학회) 2010 대한미용학회지 Vol.6 No.3

Lip balm is now one of useful cosmetics and the phenomena of well-being and LOHAS have extended to the area of cosmetics of which quality is assured. In this study, usability of Lithospermum erthrohizon (L. erthrohizon) investigated to natural cosmetic materials. Ethyl alcohol extract from L. erthrohizon was observed cytotoxicity, DPPH scavenging effect and antimicrobial activity. And lip balm made of 10% L. erthrohizon extract from ethyl alcohol and jojoba oil extract, and tested safety, usability and stability. In the result, fifty percent of fibroblast viability and brain cells were shown at the concentration of 128 mg/mL and 106 mg/mL of ethyl alcohol extracts. No extraordinary reaction or phenomena were detected like erythema (-) or allergy (-) when fore arms were patched for 48 hours with 10% lip balm made of ethyl alcohol and oil extracts. With regard to stability, there was little change in color but generation of alien substances and acidification from UV irradiation to 10% lip balm. Extremely positive responses were directed to lip balm in the order of color, feeling after application, applicability, fragrance and color reaction. DPPH scavenging activity was observed at the concentration of 5.0 mg/mL of ethyl alcohol extracts in comparison with the concentration 0.1 mg/mL of BHT as positive control material. Fractions of ethyl acetate and butanol from L. erthrohizon were measured at 12.5 mg/mL same scavenging effect as BHT. And antimicrobial activities revealed formation of clear zone with size of 9.5 mm against to S. aureus and 10 mm clear zone against to S. epidermidis at the concentration of 100 mg/mL. In conclusion, L. erthrohizon turned out to have excellent effects and antimicrobial activities as natural substance. And the lip balm made of L. erthrohizon is expected to suffice the 4 major requirements of cosmetics (safety, stability, effectiveness and usability), it is thought to be usefully employed in developing the products with natural substances that are verified of antioxidant effect and antimicrobial activity in the industry of cosmetics.

동백나무(Camellia japonica) 잎의 열수추출 조건 확립

정진호(Jin Ho Chung),이형재(Hyoung Jae Lee),이숙영(Sook Young Lee),김광상(Kwang-Sang Kim),임요섭(Yo Sup Rim),신수철(Soo Cheol Shin),정경희(Kyoung Hee Jung),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2006 한국식품과학회지 Vol.38 No.6

Effects of Low Dose γ-ray Irradiation on the Early Growth and Antioxidant Activity in Raphanus sativus L. Seeds

Eun Suk Jang(장은숙),Hyun Hwa Lee(이현화),Kwang Sang Kim(김광상),Hee Ock Boo(부희옥),Jung Hyun Sung(성정현),Ji Hae Lee(이지혜),Sook Young Lee(이숙영) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5

레몬그라스 에탄올 추출물의 3T3-L1 지방세포 분화 억제효과

조용석(Yong Seok Jo),주성민(Sung Min Ju),황금희(Keum Hee Hwang),김민숙(Kwang Sang Kim),김광상(Min Sook Kim),전병훈(Byung Hun Jeon) 한의병리학회 2019 동의생리병리학회지 Vol.33 No.1