Coagglutination에 의한 $\beta$-용혈성 연쇄구균의 혈청군 동정

정윤섭,윤양숙,김윤정,이삼열,김성광,이병수,김주덕,Chong, Yun-Sop,Yoon, Yang-Sook,Kim, Yoon-Chung,Lee, Sam-Uel Y.,Kim, Sung-Kwang,Lee, Byung-Soo,Kim, Joo-Deuk 대한미생물학회 1979 大韓微生物學會誌 Vol.14 No.1

Identification of group A $\beta$-phemolytic streptococci is very important to provide an appropriate preventive measure of possible rheumatic fever and acute glomerulonephritis. For such purpose bacitracin susceptibility of streptococci because of its simplity has been most widely used despite of its occasional faulty results. Recently, a coagglutination technique was advocated using streptococcal group specific antibodies adsorbed to protein A-containing staphylococci. This study was conducted to evaluate the coagglutination technique using reagents prepared by ourselves. The specificity, reproducibility and stability were ascertained and the following results were obtained. 1. The identification by coagglutination technique using our own reagent gave the same results compared with the Lancefield precipitation technique. The result also agreed with the Phadebact grouping. 2. There were no variation in group A and B identification due to lot difference. However, there were a few discrepant results in group C and G identification which was conducted in different days with different lots of our reagent. 3. The stability of our reagents was less satisfactory compared to the commercial product. An effort to improve the stability was considered necessary. 4. For coagglutination, it was found convenient to use supernatant of Todd-Hewitt broth incubated for 24 hours. Both parafin-ringed slide glass and RPR card gave comparable results and the former could be used when the latter is not available.

심부전 모델에서 실험적 심근성형술의 단기 혈역학적 효과에 관한 연구

정윤섭,염욱,이창하,김욱성,이영탁,김원곤,Jeong, Yoon-Seop,Youm, Wook,Lee, Chang-Ha,Kim, Wook-Seong,Lee, Young-Tak,Kim, Won-Gon 대한흉부심장혈관외과학회 1999 Journal of Chest Surgery (J Chest Surg) Vol.32 No.3

배경: 역동적 심근성형술에서 골격근 수축에 의한 단기 혈역학적 변화를 관찰하고 이에 미치는 인자를 분석하고자 하였다. 대상 및 방법: 이를 위해 20-30kg 사이의 한국산 잡견 10마리를 두 그룹으로 나눠 심장 상태를 정상과 심부전 상태로 구분하였고 골격근 상태도 활성도 및 수축력의 차이가 나도록 구분하였다. 그룹 A에서는 5마리의 정상 심장상태의 실험견에 심근성형술을 시행한 뒤 8주후 혈역학 검사를 실시하였고, 그룹 B에서는 5마리의 실험견에 8주동안 매주 1 mg/kg의 doxorubicin을 주입하여 만성 심부전 상태를 만들면서 동시에 좌측 광배근의 사전 조건화를 위한 근육훈련을 한후 심근성형술을 시행하고 바로 혈역학 검사를 실시하였다. 결과: 그룹 A의 정상 심장 상태에서 광배근 수축으로 평균 우심방 압력을 제외한 나머지 좌심실 혈역학 지수에는 유의한 변화가 없었다. 그룹 B에서는 광배근 수축으로 심박출량(cardiac output; CO)은 16.7$\pm$7.2%, 좌심실 압력발생 속도(positive pressure development rate of left ventricle; dp/dt)는 9.3$\pm$3.2%, 일회 심박출량(stroke volume; SV)은 16.8$\pm$8.6%, 좌심실 박출작업량(left ventricular stroke work; SW)은 23.1$\pm$9.7% 증가하였고, 좌심실 이완기말압(left ventricular end-diastole pressure; LVEDP)은 32.1$\pm$4.6%, 평균 폐동맥쐐기압(mean pulmonary capillary wedge pressure; mPCWP)은 17.7$\pm$9.1% 감소하였다(p<0.05). 그러나 그룹 A에서 imipramine을 7.5 mg/kg/hour의 속도로 34$\pm$2.6분 투여하여 CO이 17.5$\pm$2.7%, 좌심실 수축기압(left ventricular systolic pressure)이 15.8$\pm$2.5% 감소하고 LVEDP가 54.3$\pm$15.2% 증가한 일시적 급성 심부전 상태를 유도한 뒤(p<0.05), 이 상태에서 광배근을 자극하였더니 CO은 4.5$\pm$1.8%, dp/dt는 3.1$\pm$1.1%, SV는 5.7$\pm$3.6%, SW는 6.9$\pm$4.4% 증가하였고, LVEDP는 11.7$\pm$4.7% 감소하였다(p<0.05). 그룹 A의 급성 심부전 상태와 그룹 B의 만성 심부전 상태에서 모두 광배근 수축으로 변화한 CO, dp/dt, SV, SW, LVEDP 같은 좌심실 혈역학 지표들의 변화의 폭을 비교하면 그룹 B에서 그룹 A에서보다 더 컸다(p<0.05). 그룹 A에서 유도된 급성 심부전 상태와 그룹 B의 만성 심부전 상태가 CO, dp/dt, SV, SW, LVEDP 같은 좌심실 혈역학 지표들 면에서 통계학적으로 차이가 없고(p>0.05), 육안적으로 광배근을 관찰하였을 때 그룹 A에서는 광배근의 유착 및 염증소견이 모두에서 있었고 그중 2마리에서는 광배근의 수축을 목격할 수 없었던 반면, 그룹 B에서는 5마리 모두에서 광배근이 활발하게 수축하였다는 점을 함께 고려하면 그룹 B에서의 더 큰 증폭 효과가 광배근의 활성도 및 수축력의 차이로부터 기인한다고 평가할 수 있다. 결론: 이상에서 역동적 심근성형술의 수축기 혈역학적 변화는 심부전 상태에서만 긍정적인 개선 효과를 나타내며, 그 효과의 극대화를 위해서는 근육의 수축력을 유지하는 것이 매우 중요함을 알 수 있다. Background: To evaluate the short-term effect of dynamic cardiomyoplasty on circulatory function and detect the related factors that can affect it, experimental cardiomyoplasties were performed under the state of normal cardiac function and heart failure. Material and Method: A total of 10 mongrel dogs weighing 20 to 30kg were divided arbitrarily into two groups. Five dogs of group A underwent cardiomyoplasty with latissimus dorsi(LD) muscle mobilization followed by a 2-week vascular delay and 6-week muscle training. Then, hemodynamic studies were conducted. In group B, doxorubicin was given to 5 dogs in an IV dose of 1 mg/kg once a week for 8 weeks to induce chronic heart failure, and simultaneous muscle training was given for preconditioning during this period. Then, cardiomyoplasties were performed and hemodynamic studies were conducted immediately after these cardiomyoplasties in group B. Result: In group A, under the state of normal cardiac function, only mean right atrial pressure significantly increased with the pacer-on(p<0.05) and the left ventricular hemodynamic parameters did not change significantly. However, with pacer-on in group B, cardiac output(CO), rate of left ventricular pressure development(dp/dt), stroke volume(SV), and left ventricular stroke work(SW) increased by 16.7${\pm}$7.2%, 9.3${\pm}$3.2%, 16.8${\pm}$8.6%, and 23.1${\pm}$9.7%, respectively, whereas left ventricular end-diastole pressure(LVEDP) and mean pulmonary capillary wedge pressure(mPCWP) decreased by 32.1${\pm}$4.6% and 17.7${\pm}$9.1%, respectively(p<0.05). In group A, imipramine was infused at the rate of 7.5mg/kg/hour for 34${\pm}$2.6 minutes to induce acute heart failure, which resulted in the reduction of cardiac output by 17.5${\pm}$2.7%, systolic left ventricular pressure by 15.8${\pm}$2.5% and the elevation of left ventricular end-diastole pressure by 54.3${\pm}$15.2%(p<0.05). With pacer-on under this state of acute heart failu e, CO, dp/dt, SV, and SW increased by 4.5${\pm}$1.8% and 3.1${\pm}$1.1%, 5.7${\pm}$3.6%, and 6.9${\pm}$4.4%, respectively, whereas LVEDP decreased by 11.7${\pm}$4.7%(p<0.05). Comparing CO, dp/dt, SV, SW and LVEDP that changed significantly with pacer-on, both under the state of acute and chronic heart failure, augmentation widths of these left ventricular hemodynamic parameters were significantly larger under the state of chronic heart failure(group B) than acute heart failure(group A)(p<0.05). On gross inspection, variable degrees of adhesion and inflammation were present in all 5 dogs of group A, including 2 dogs that showed no muscle contraction. No adhesion and inflammation were, however, present in all 5 dogs of group B, which showed vivid muscle contractions. Considering these differences in gross findings along with the following premise that the acute heart failure state was not statistically different from the chronic one in terms of left ventricular parameters(p>0.05), the larger augmentation effect seen in group B is presumed to be mainly attributed to the viability and contractility of the LD muscle. Conclusion: These results indicate that the positive circulatory augmentation effect of cardiomyoplasty is apparent only under the state of heart failure and the preservation of muscle contractility is important to maximize this effect.

Ampicillin과 Chloramphenicol 내성 Salmonella typhimurium 분리의 증가

정윤섭,한상순,권오헌,이삼열,정태화,Chong, Yun-Sop,Han, Sang-Soon,Kwon, Oh-Hun,Lee, Samuel.Y.,Jung, Tae-Hwoa 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.1

Salmonella typhi infection, which was the most frequent enteric infection in Korea, has been decreasing, while the infection of other serogroups of Salmonella has been increasing since the later part of 1970s. In 1986, the number of serogroup B isolated by us increased to 46, which corresponds 21.1% of all enteropathogenic bacteria isolated from stool specimens. Salmonella isolates resistant to antimicrobial agents were extremly rare in Korea, in the 1970s. However, 7 of 13 serogroup B isolates showed resistance to ampicillin or to chloramphenicol in 1984. Among the serogroup B isolates in 1986, 71.7% and 69.6% were resistant to ampicillin and to chloramphenicol respectively. The minimum inhibitory concentrations of ampicillin and chloramphenicol against these isolates were >$128\;{\mu}g/ml$ and $128\;{\mu}g/ml$ respectively.

Hydrogen Sulfide 생성 Escherichia coli 및 Salmonella paratyphi-A의 분리

정윤섭(Yun Sop Chong),김진주(Jin Ju Kim ),권오헌(Oh Hun Kwon),이삼열(Samuel Y. Lee),윤진우(Jhin Woo Yoon),홍천수(Chun Soo Hong ),이복권(Bok Kwon Lee) 大韓微生物學會 1985 大韓微生物學會誌 Vol.20 No.2

형상기억합금 와이어 점진적 전류인가 실험과 실험 장비

정윤섭(Y. S. Chung),김종호(J. Kim),이지형(J.-H Lee),이한얼(H. Lee),로드리그 휴고(H. Rodrigue) 한국생산제조학회 2017 한국생산제조시스템학회 학술발표대회 논문집 Vol.2017 No.12

Coagglutination에 의한 β-용혈성 연쇄구균의 혈청군 동정

정윤섭(Yunsop Chong),윤양숙(Yang Sook Yoon),김윤정(Yoon Chung Kim),이삼열(Samuel Y. Lee),김성광(Sung Kwang Kim),이병수(Byung Soo Lee),김주덕(Joo-Deuk Kim) 大韓微生物學會 1979 大韓微生物學會誌 Vol.14 No.1

성인 장염 환자에서의 Yersinia enterocolitica분리 3예

정윤섭(Yunsop Chong),이희주(Hee Joo Lee),이삼열(Samuel Y. Lee),강진경(Jin Kyung Kang),문영명(Young Myuong Moon) 大韓微生物學會 1980 大韓微生物學會誌 Vol.15 No.1

한국인 임산부에서의 Group B streptococci 감염에 대한 연구

박이석(Lee Suk Park),서경(Kyung Seo),김세광(Sei Kwang Kim),박용원(Yong Won Park),정현용(Hyun Yong Jung),정윤섭(Yun Sop Chong),이경원(Kyung Won Lee) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.9

목적: 분만을 위해 내원한 임신부와 신생아를 대상으로 B군 연쇄구균의 보균율을 알아보고자 본 연구를 시행하였다. 연구방법: 본 연구는 1991년 4월부터 1992년 8월까지 신촌 세브란스병원 산부인과에 분만을 위해 내원한 단태아 임신부와 이들이 분만한 신생아 153쌍을 대상으로 하였다. 검체는 임신부의 질하부와 자궁경부 및 신생아의 이관과 인후부위에서 채취하여 Todd-Hewitt broth(Difco) 선택배지에 접종한 후 48시간 동안 배양하여 B군 연쇄구균을 동정하였다. 결과: 임신부와 신생아의 B군 연쇄구균 보균율은 각 각 2.61%(4/153)와 0%(0/4)이었으며, 대상 신생아의 B군 연쇄구균 양성결과 1 예에서 패혈증이 의심되었다. 결론: 한국인 임산부의 B군 연쇄구균 감염률은 외국에 비해 낮으며, 그 이유는 인종차이 내지는 사회-경제적 혹은 생활방식의 차이와 관련이 있을 것으로 사료된다. Objective: To evaluate the degree of streptococcal colonization in Korean pregnant women. Methods: The study comprised of 153 singleton pregnant women who visited Severance Hospital for delivery, and their neonates. Specimens for GBS culture were collected by a sterile cotton swab from lower vagina and cervix of pregnant women, and from ear canal and throat of neonates. They were first cultured for 48 hours in Todd-Hewitt broth and then subcultured onto Tryptose blood agar plates(Difco). Group B streptococci were confirmed by the presence of β-hemolysis and a positive reaction with Phadebact group B Streptococci reagent(Karo Biodiagnostics AB, Huddinge, Sweden). Results: The prevalence of positive cultures in pregnant women and neonates were 2.61%(4/153) and 0%(0/4), respectively. In the study population there was a case of suspicious group B streptococcual sepsis in an infant whose mother was colonized. Conclusions: In our study the GBS colonization rate in Korean pregnant women was significantly lower than that of other countries. The reason for this difference may be associated with a racial differences, or social factors such as socio-economic status or a life style.

인공 신경망 기반 차 실내 열적 쾌적성 예측

이정훈(Jeonghoon Lee),고원식(Wonsik Go),김중재(Joongjae Kim),성현제(Hyunje Sung),장동희(Dongeui Chang),장길상(Kilsang Jang),손일국(Ilguk Son),양희주(Huiju Yang),안준철(Junchul An),이준민(Junmin Lee),최준영(Junyoung Choi),정윤섭(Yunsub C 한국자동차공학회 2019 한국자동차공학회 부문종합 학술대회 Vol.2019 No.5

변형적 Carpentier 방법에 의한 Ebstein 기형의 수술적 교정 -1 례 보고-

이건,김웅한,이창하,나찬영,정윤섭,정도현,김수철,이영탁,김종환,홍승록,박영관,Lee, Gun,Kim, Woong-Han,Lee, Chang-Ha,Na, Chan-Young,Jeong, Yoon-Seop,Jeong, Do-Hyun,Kim, Soo-Cheol,Lee, Young-Tak,Kim, Chong-Whan,Kim, Sung-Nok,Park, Young- 대한흉부심장혈관외과학회 1998 Journal of Chest Surgery (J Chest Surg) Vol.31 No.2

Ebstein 기형은 복잡 심기형중 하나로 치료 또한 비정상적인 삼첨판막의 교정 또는 판막치환 등으로 방법이 다양하다. 이질환의 병리소견은 삼첨판의 중격엽과 후엽이 하방전위되어서 심방화된 심실을 형성하는 것으로 수술적 목표는 삼첨판의 기능이상을 교정하고 심방화된 우심실을 주름성형하는 것이다. 본원에서는 매우 심한 삼첨판막 폐쇄 부전증이 동반된 Carpentier type A의 Ebstein기형으로 진단된 12개월된 여아에 대해 심방화된 우심실을 수직방향으로 주름성형하고 판막을 새로이 형성된 판륜에 재부착시키는 이른바 변형적 Carpentier방법으로 성공적으로 수술하였다. 수술후 환아는 심장의 크기가 매우 줄어들었고 심초음파상 미미한 정도의 삼첨판 폐쇄부전증만 남아 술후 14일째 퇴원하였다. Ebstein's anomaly is a complex malformation that can be treated by various surgical techniques, either repair or replacement of the abnormal tricuspid valve, with variable results. The essence of the malformation is the downward displacement of the septal and posterior leaflets into the ventricle, resulting in the formation of an atrialized portion of the right ventricle. The aim of surgical repair is to correct the tricuspid valve dysfunction and to plicate the atrialized portion of the right ventricle A 12-months old female was admitted with the diagnosis of Carpentier type A of Ebstein's anomaly with severe tricuspid regurgitation. She successfully underwent operation with vertical plication of right ventricle and reimplantation of tricuspid leaflets. Postoperatively cardiac size was significantly reduced and tricuspid regurgitation was trivial in echocardiography. She was diacharged the 14th postoperative day.