적외선우유분석용 (赤外線牛乳分析用 ) 생유시료의 (生乳試料) 보존성에 관한 연구 - I. 생유시료의 저장중 변화와 보존기간에 관한 연구

권일경,장준환,장영호,허철성,김선기,김현욱 ( Il Kyung Kwon,Jun Hwan Chang,Young Ho Chang,Chul Sung Heo,Sun Ki Kim,Hyun Uk Kim ) 한국낙농학회 1982 韓國酪農學會誌 Vol.4 No.1

심각한 소아 · 청소년 강박장애로 입원한 환아들의 특징

황준원(Jun-Won Hwang),김붕년(Boong-Nyun Kim),유소영(So-Young You),장준환(Jun-Hwan Chang),신민섭(Min-Sup Shin),조수철(Soo-Churl Cho),홍강의(Kang-E Hong) 대한소아청소년정신의학회 2004 소아청소년정신의학 Vol.15 No.2

CJ-11555의 Sprague-Dawely 랫드를 이용한 단회 및 4주 반복경구투여 독성시험

김일환(Il-Hwan Kim),이성학(Sung-Hak Lee),최재묵(Jae-Mook Choi),박지은(Jie-Eun Park),김덕열(Deog-Yeor Kim),노현정(Hyun-Jung Noh),김택로(Taek-Rho Kim),이상호(Sang-Ho Lee),김영훈(Young-Hoon Kim),김진완(Jin-Wan Kim),장준환(Jun-Hwan Chang 한국독성학회 2004 Toxicological Research Vol.20 No.2

This study was to investigate single and repeated-dose toxicities of CJ-11555, an anticirrhotic agent, in Sprague-Dawley (SD) rats. In single-dose oral toxicity study, the test article were<br/> administered once by gavage to males and females at dose levels of 0 and 2,000 mg/kg. No dead animals and abnormal necropsy findings were found in control and CJ-11555 treated group. Therefore, the approximate lethal dose of CJ-11555 was considered to be higher than 2,000 mg/kg in rats. In the 4-week repeated oral toxicity study, the test article was administered once daily by gavage to<br/> male and female rats at dose levels of 0, 10, 50 and 200 mg/kg/day for 4-weeks. In clinical signs, yellow-colored urine and yellow hair coat were observed in the 50 and 200 mg/kg male and female<br/> groups. In hematology, erythrocyte count and hemoglobin were significantly decreased in the 200mg/kg male and female groups. In serum biochemistry, total cholesterol was significantly increased and aspartate aminotransferase (AST) was significantly decreased in the 50 or 200 mg/kg male and female groups. In histopathological examinations, centrilobular hepatocellular hypertrophy in the liver, congestion and pigmentation in the spleen, hyaline droplets in the kidney were observed in the 50 and 200 mg/kg male and female groups. In toxicokinetic study, CJ-11555 was dose-dependent in systemic exposure and showed better absorption in female with minimum accumulation after multidosing. Based on these results, it was concluded that the 4-week repeated oral dose of CJ-11555<br/> resulted in the suppression of AST activity and centrilobular hepatocellular hypertrophy in both sexes at a dose level of 50 or 200 mg/kg/day. The target organ was estimated to be liver, spleen and male's kidney. The no-observed-adverse-effect level (NOAEL) for CJ-11555 in rats following gavage for at least 4-week is 10 mg/kg/day.

CJ-11555의 Sprague-Dawely 랫드를 이용한 단회 및 4주 반복경구투여 독성시험

김일환(Il-Hwan Kim),이성학(Sung-Hak Lee),최재묵(Jae-Mook Choi),박지은(Jie-Eun Park),김덕열(Deog-Yeor Kim),노현정(Hyun-Jung Noh),김택로(Taek-Rho Kim),이상호(Sang-Ho Lee),김영훈(Young-Hoon Kim),김진완(Jin-Wan Kim),장준환(Jun-Hwan Chang 한국독성학회 2004 Toxicological Research Vol.20 No.1

This study was to investigate single and repeated-dose toxicities of CJ-11555, an anticirrhotic agent, in Sprague-Dawley (SD) rats. In single-dose oral toxicity study, the test article were administered once by gavage to males and females at dose levels of 0 and 2,000 mg/kg. No dead animals and abnormal necropsy findings were found in control and CJ-11555 treated group. Therefore, the approximate lethal dose of CJ-11555 was considered to be higher than 2,000 mg/kg in rats. In the 4-week repeated oral toxicity study, the test article was administered once daily by gavage to<br/> male and female rats at dose levels of 0, 10, 50 and 200 mg/kg/day for 4-weeks. In clinical signs, yellow-colored urine and yellow hair coat were observed in the 50 and 200 mg/kg male and female<br/> groups. In hematology, erythrocyte count and hemoglobin were significantly decreased in the 200mg/kg male and female groups. In serum biochemistry, total cholesterol was significantly increased and aspartate aminotransferase (AST) was significantly decreased in the 50 or 200 mg/kg male and female groups. In histopathological examinations, centrilobular hepatocellular hypertrophy in the liver, congestion and pigmentation in the spleen, hyaline droplets in the kidney were observed in the 50 and 200 mg/kg male and female groups. In toxicokinetic study, CJ-11555 was dose-dependent in systemic exposure and showed better absorption in female with minimum accumulation after multidosing. Based on these results, it was concluded that the 4-week repeated oral dose of CJ-11555 resulted in the suppression of AST activity and centrilobular hepatocellular hypertrophy in both sexes at a dose level of 50 or 200 mg/kg/day. The target organ was estimated to be liver, spleen and male's kidney. The no-observed-adverse-effect level (NOAEL) for CJ-11555 in rats following gavage for at least 4-week is 10 mg/kg/day.

심각한 소아 ${\cdot}$ 청소년 강박장애로 입원한 환아들의 특징

황준원,유소영,장준환,신민섭,조수철,홍강의,김붕년,Hwang, Jun-Won,You, So-Young,Chang, Jun-Hwan,Shin, Min-Sup,Cho, Soo-Churl,Hong, Kang-E,Kim, Boong-Nyun 대한소아청소년정신의학회 2004 소아청소년정신의학 Vol.15 No.2

연구목적:소아·청소년기 발병 강박장애는 낮은 약물반응, 높은 공존장애율, 강한 유전적 경향성을 가진다고 알려져 왔다. 그러나 현재까지 국내에서 소아·청소년기 발병 강박장애에 대한 연구는 극히 미미하다. 이에 본 연구에서는 서울대학교병원 소아·청소년 정신과 병동에 입원한 강박장애 아동들을 대상으로 임상양상, 유전부하, 약물 반응양상, 퇴원후의 경과등을 조사하여, 심각한 증상을 보이는 소아·청소년 강박장애 군의 특성을 파악하려 하였다. 방 법:최근 9년사이에 서울대병원 소아·청소년 정신과에 입원한 환아 20명(남 16, 여 4)의 자료를 조사하였고 이를 대상으로 임상적 특성을 조사하였다. 진단 및 임상 상의 정확성을 유지하기 위해 의무기록지, 심리학적 보고서, 가족 면담 자료, 간호 보고서를 모두 고려하였고, 퇴원시 확정된 진단을 일차진단으로 하였다. 결 과:1) 환아의 성비는 남성이 4:1로 우위였다. 2) 강박사고에 있어서 가장 흔한 형태는 병적의심이었고, 이어서 더러움에 대한 공포, 공격적 사고, 대칭에의 요구, 성적 강박 사고였다. 강박행동에서 가장 흔한 내용은 확인, 씻기, 숨쉬기, 움직임, 대칭, 반복적 질문, 물건수집, 정신적 강박행동 등이었다. 3) 가장 흔한 공존 장애는 우울증이었고, 그 다음으로는 다른 불안장애, 틱장애, 품행장애, 반항장애였다. 드물게 강박사고에 대한 현실검증력이 손상된 것으로 판단되는 아동도 발견되었다. 4) 정신과 질환에 대한 가족력을 살펴본 결과, 17 가족(85%)에서 2차 친척 중 정신과 장애를 앓는 사람이 발견되었다. 그리고, 강박 스펙트럼 장애로 의심되는 친척을 둔 아동이 전체의 45%(9명)이었다. 5) 약물치료로서 사분의 삼 정도의 환아들(75%)이, SSRI와 항정신병약물을 함께 복용하고 있었고, 치료 반응상 CGI에서 중등도 호전(moderate improved) 이상을 보이는 경우가 75%로 파악되었다. 6) 퇴원 후의 외래 추적 결과, 지속적인 증상의 관해를 보인 경우는 5명(25%)이었고, 부분적인 증상을 가진 채 만성적으로 지속되는 경우가 10명(50%)이었다. 결 론:심각한 소아·청소년 강박장애약물 환아들의 임상상을 국내에서는 최초로 체계적으로 조사하였다. 그 결과, 높은 공존장애율, 높은 정신과장애의 가족력, 높은 항정신병약물 복용을 보이는 것으로 조사되었다. 증상면에서도 공격적-성적 강박사고의 비율이 높았으며, 숨쉬기, 움직이기 등의 특이한 강박행동이 발견되었다. 치료로서 사분의삼 정도의 환아들(75%)이, SSRI와 항정신병약물을 함께 복용하고 있었고, 치료 반응상 CGI에서 중등도 호전(moderate improved) 이상을 보이는 경우가 75%로 파악되었다. Objectives : Child and adolescent onset obsessive compulsive disorder(C-OCD) is known to be associated with poor drug response, high comorbid rate and strong genetic tendencies. Till now studies for C-OCD have been very rare in Korea. We conducted this study to investigate the informations about clinical features, familial psychiatric loading, treatment profiles and course of server C-OCD from the retrospective analysis of inpatient data of Seoul National University Children's Hospital. Method : Retrospective chart review and data analysis was performed. Twenty(male 16 : female 4) patients with final C-OCD diagnosis by DSM-IV at discharge from 1994 to 2002 were found and their medical chart, psychological data, family interview data and nursing reports were collected and analyzed. Results : 1) The sex ratio of C-OCD was male dominant(4:1). 2) Phenomenological, most common obsession was pathologic doubt, contamination fear, followed by aggressive obsession, need for symmetry, sexual obsession, most common compulsion was checking and washing, followed by breathing, movement, symmetry, repetitive asking, hoarding, mental compulsion. 3) Most common comorbid diagnosis was depression. Other axis-I diagnosis associated OCD were anxiety disorder, tic disorder, conduct and oppositional defiant disorder and psychosis. 4) Regarding psychiatric familial loadings, 17 patients(85%) had relatives with psychiatric disorders, OC-spectrum disorders(OCD or OCPD) were found in 9 patients(45%). 5) The majority of patients(75%) have received SSRI and antipsychotics treatment. The response rate above 'moderate improved" by CGI was 75%. 6) During follow-up period in outpatient clinic, five patients(25%) showed continuous complete remission, 10 patients (50%) did residual symptoms with chronic course. Conclusion : This seems to be the first systemic investigation of severe pediatric OCD patients in Korea. The children & adolescents with severe OCD in inpatient-setting showed the high comorbid rate, familial psychiatric loading, and combined pharmacotherapy with antipsychotics, As for symptoms, high rate of aggressive-sexual obsession and atypical compulsions like breathing and moving was reported in this study. Severe pediatric OCD patients, however, responded well to the combined SSRI and antipsychotics regimen.

방선균에 의해 생산된 항 MRSA 항생물질 AM3 의 구조 연구

임융호(Yoong Ho Lim),장준환(Jun Hwan Chang),김종훈(Jong Hoon Kim),서정우(Jung Woo Suh),정재경(Jae Kyung Jung),이철훈(Chul Hoon Lee) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.6

In order to find a potent anti-methicillin resistant Staphylococcus aureus (MRSA) antibiotic, actinomycetes isolated from the samples collected in Korean marine silt were screened. From the culture broth of the isolated Streptamyces strain AM045, a substance showing excellent biological activity against MRSA was found, isolated and named AM3. The compound showed strong activities against MRSA, S. epideridis, E. faecium and E. faecalis, which were better than those of vancomycin and teicoplanin. Unfortunately, AM3 was identified as Actinomycin V. However, this paper reports the three dimensional study of AM3 based on high resolution nmr and Computer Aided Molecular Modeling(CAMM), and the fact that the structure of the pentapeptide lactone ring with oxo-proline in chloroform solution does not have $quot;C conformation$quot; any more.

강박장애 환자에서의 안와전두피질 용적의 2년 추적 연구

김성년,강도형,유소영,노규식,장준환,최정석,하태현,권준수,Kim, Sung-Nyun,Kang, Do-Hyung,Yoo, So-Young,Roh, Kyu-Sik,Chang, Joon-Hwan,Choi, Jung-Seok,Ha, Tae-Hyon,Kwon, Jun-Soo 대한불안의학회 2006 대한불안의학회지 Vol.2 No.2

Objective : This study was designed to examine the volumetric abnormality of orbitofrontal cortex (OFC) and its change after 2 years of pharmacotherapy in obsessive compulsive disorder (OCD) patients. Method : Volumetric magnetic resonance imaging studies were conducted in 15 OCD patients and 13 normal volunteers. For 2 years, all patients took at least one serotonin reuptake inhibitor and atypical antipsychotics were used as an augmentation therapy in most patients. The follow-up MRI studies were conducted after the pharmacotherapy and OFC volumes were measured by the manual region of interest method. Results : Bilateral OFC volumes of 15 OCD patients were significantly greater than those of the normal volunteers before the treatment. After 2 years of the treatment, significant decrease was observed in bilateral OFC volumes of OCD patients to the extent that left OFC volume of OCD patients was not different from that of the normal volunteers. Conclusion : This finding suggests that OFC is directly related to the pathophysiology of obsessive compulsive disorder.

말뚝으로 보강된 탄성지반상에 놓여 있는 연속기초의 해석

장준환,신수곤,장병순 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.59 No.-

지반이 다소 연약하여 건물 기초를 말뚝으로 보강된 연속줄기초로 설계할 경우, 탄성지반을 무시하고, 말뚝을 탄성지점으로 한 연속보로 처리하는 것이 보통이다. 그러나 실제 기초에서는 지반반력과 말뚝반력을 모두 무시할 수 없는 경우가 많다. 본 논문은 지반반력과 말뚝반력을 동시에 고려하고 n경간의 탄성지점을 가지는 탄성지반상의 보에 대한 해석을 반무한장보와 유한장보로 구분하여 계시하고, 하중 재하위치에 따라 반무한장보 및 유한장보로 해석해야 할 범위를 제시하였다 In this paper, the method of analysis for the girder supported with piles on the elastic soilmedium is proposed. The girder supports with piles on the elastic soil medium is often used as those of crane rail at a ship yard. For the purpose of practical design, these girders were often regarded as continuous beams elastically supported with piles. Actually, the method of ana1ysis for these girders should be considered as analysis of girder supported with piles on the elastic soil medium. From the proposed method, we can calculate bending moment and shearing force of these girders, after obtaining the reaction of piles by calculating the deflection of pile support point due to external load and pile reaction.

한국모유아 장내에 분포하는 Bifidobacteria에 관한 연구

장준환,김현욱,권일경 한국낙농학회 1983 韓國酪農學會誌 Vol.5 No.2

이 연구는 한국유아의 장내에 Bifidobacteria 생태에 관한 지식을 얻고자 수행되었으며 모유아의 변에서 분리한 Bifidobacteria의 분포 및 형태적, 생리적 성질을 연구한 결과는 다음과 같다. 1. Bifidobacteria선택분리 배지로서 MCNC 배지가 성공적으로 사용될 수 있다고 믿어진다. 2. B. bifidum의 균락은 MCNC 평판배지에서 Pale blue색을 띠었고 B. longum은 deep blue색을, B. liberorum은 pale blue이며 중앙부가 deep blue색을 각각 나타내었다. 3. 14명의 모유아의 변에서 84개의 비휘더스균주가 분리되었고. 분리동정한 결과 B. bifidum이 70%, B. longum이 19%, B. liberorum이 2%, 미동정 균주가 각각 9%였다. 4. 한국모유아의 장내에서는 B. bifidum이 우점하고 있으며, 모유포유에 의해 증식이 상당히 촉진되는 것으로 믿어진다. 5. B. bifidum 균주들은 pH 9.6, NaCl 2%, Tween 80에서의 성장실험에서 각기 다른 반응을 나타내었고, B. longum과 B. liberorum 균주들은 Tween 80에 의해 성장이 억제되지 않았으며, B. liberorum과 균주는 NaCl 2%에서 성장이 억제되었다.

Fourier급수를 이용한 단순 지지 박스형 곡선교의 정적거동에 관한 연구

장병순,장준환,김수영 釜山大學校生産技術硏究所 1998 生産技術硏究所論文集 Vol.55 No.-

일반적으로 곡선형교는 휨과 비틂이 연성거동하므로 그 해석절차나 설계가 복잡하다. 그러므로 곡선교의 해석을 쉽게 하기위해 본 연구에서는 단순지지 박스형 곡선교에 대하여 Fourier 급수로 표현된 변위를 Energy Method로 정식화시키고 이들을 이용하여 간단히 단면력을 구할수 있는 방법을 제시하였다. 또한 해석시 재하하중과 곡률반경의 변화에 다라 단면력의 영향선을 구하였으며 특히 휨모멘트에 대해서는 직선교의 영향선도 함께 나타내었다. In general, a curved grider birdge is complicated for design and analysis because of coupled behaviour about flexure and torsion. In this study, to analyze behaviour of coupling of bending and torsion, Energy Method using fourier Series is adopted. Also, a simple method to obtain the stress resultants of curved girders is proposed. Stress resultants and displacements due to changing load and curvature are presented by the influence line.