반추가축영양 : Lignosulfonate처리 대두박의 반추위 내 미생물 발효특성에 미치는 영향

장문백 ( Moon Baek Chang ),이승헌 ( Seung Heon Lee ),이훈정 ( Hun Jong Lee ),배귀석 ( Gui Seck Bae ),박제환 ( Je Hwan Park ) 한국동물자원과학회 ( 구 한국축산학회 ) 2010 한국축산학회지 Vol.52 No.5

본 연구는 반추가축에 급여되는 사료원 중 종류별, 수준별 lignosulfonate 처리 대두박의 반추위 내 undegradable protein (UDP) 효율을 알아보기 위하여 실험 I 에서 전체 4종의 lignosulfonate (Desulfonated, Na, Ca, Solution)를 각각 수준별 (2, 4, 8%)로 처리하여 전체 12종의 lignosulfoanted soybeanmeal (LSBM)에 대한 반추위 내 미생물 발효 특성의 미치는 영향을 알아보기 위해 in vitro 시험이 실시되었다. 실험 H에서는 실험 I 에서 대두박의 단백질 보호 효과가 가장 높은 LSBM Na 2% 처리구와 LSBM Solution (LSBM Sol) 2% 처리구 그리고 열처리 LSBM (LSBM Heat) 2%를 이용하여 rumen simulation continuous culture (RSCC) system을 이용한 반추위 내 미생물 발효 특성에 대한 연구가 실시되었다. 시험 I in vitro 시험에서 모든 LSBM 처리구에서 전체 배양시간 평균 pH수준, gas 생성량, NH3-N 농도, 건물소화율, VFA 생성량 그리고 ADF 소화율은 배양시간이 지남에 따라 대조구에 비해 낮은 결과를 나타내었다(p<0.05) 반추위 내 미생물단백질 합성량은 대조구가 LSMB 처리구 보다 통계적으로 높은 결과를 나타내었으며 (p<0.05), LSBM 수준이 증가할수록 반추위 내 미생물 단백질 합성량은 감소하는 경향을 나타내었다. UDP 함량은 대조구보다 LSBM 처리구에서 높은 결과를 나타내었으며(p<0.05), 특히 LSBM Sol 처리구는 대조구에 비해 각각 2%, 4% 그리고 8% 처리군에서 11.18%, 11.54% 그리고 29.11%의 UDP 함량이 증가 하였다. 시험 Ⅱ는 RSCC system을 이용하여 반추위 내 미생물 발효특성에 대한 시험이 실시되었다. 시험사료는 대조구와 In vitro 시험 중 대조구와 UDP 효과가 높은 LSBM Na 2%, LSBM Sol 2% 그리고 LSBM Heat 2% 처리한 사료 4처리 4반복(4x4 Latin squire)에 의해 15일간 시험이 실시되었으며, 배양 마지막 3일 동안의 sample을 이용하여 분석을 실시하였다. 전체 배양 기간 중 모든 처리구에서 적응기간 동안 불규칙적인 반추위 미생물 발효 특성을 보였으나 배양 12일 이후 안정된 발효특성을 나타내었다. Sampling 기간 동안의 각 처리간의 평균 pH는 모든 LSBM 처리구에서 대조구보다 높았다(p<0.05). NH3-N 함량, VFA 함량 그리고 반추위 내 미생물 단백질 합성량은 LSBM 처리구가 대조구에 비해 낮은 결과를 나타내었다(p<0.05). UDP 함량은 대조구, LSBM Heat 2%, LSBM Na 2% 그리고 LSBM Sol 2%에서 각 41.55, 43.49, 45.28 그리고 43.52%로 모든 LSBM 처리구가 대조구에 비해 유의하게 높은 경향을 나타내였다(p<0.05). 시험 I 과 시험Ⅱ에서 UDP 함량은 LSBM 처리구에서 lignosulfonate 처리 수준이 증가할수록 증가하는 경향을 나타내었고, 모든 LSBM 처리구는 대조구에 비해 높은 UDP 함량을 나타내었다. 이와 같은 결과는 사료원으로써 대두박에 대한 lignosulfonate 처리가 coating 작용과 열처리에 의한 물리·화학적 효과로 인하여 대두박의 단백질 분해율을 저해 시킨 것으로 사료된다. 그러나 LSBM 처리군 중 건물소화율과 반추위 내 미생물단백질 합성량은 LSBM 2% 처리구에서 가장 높은 결과를 나타내였다. 따라서, 반추가축 사료내 LSBM 첨가가 In vitro와 RSCC system을 이용한 반추위내 미생물발효 특성 시험에서 반추위 내에서 by-pass protein 함량을 증가시키는 결과를 나타내었으며, 추후 착유우를 이용한 LSBM 급여를 시험을 통하여 유단백질 증진효과에 대하여 추가적인 평가가 이루어져야 할 것으로 사료된다. This study was conducted to investigate the effects on fermentation characteristics of rumen microorganism by different types and levels of lignosulfonate treated soybean meal (LSBM) in in vitro test and rumen simulation continuous culture (RSCC) system in dairy cows. The experiment I was control and 12 treatments (each with 3 replications) in vitro test to demonstrate composition of different types of treatments with lignosulfonate (Desulfonate, Na, Ca and solution) and levels (2, 4 and 8%) of soybean meal in the dairy cow diet. LSBM source treatments in the dairy cow diet showed pH value, NH)-N concentration and total YF A concentration lower than control at all levels and incubation times (p<0.05). Dry matter digestibility of LSBM source treatments showed lower than control (p<0.05). Gas production and rumen microbial synthesis was decreased by rumen microbial fermentation for incubation times. Undegradable protein (UDP) concentration of all LSBM treatments was decreased for incubation times, and significantly higher than control (p<0.05). In the experiment Il compared diets of the control, LSBM Na 2%, LSBM Sol 2%, which are high performance to Undegradable protein (UOP) concentration experiment I in vitro test, and heated treatment Iignosulfonate (LSBM Heat) 2% in the dairy cow diet from four station RSCC system (4x4 Latin square). A rumen microbial fermentation characteristic was stability during 12-15 days of experimental period in all treatments. The pH value of LSBM treatments was higher than control treatment (p<0.05). The NH)-N concentration, YFA concentration and rumen microbial synthesis of LSBM treatments were lower than control (p<0.05). The undegradable protein (UDP) showed LSBM Na 2% (45.28%), LSBM Sol 2% (43.52%) and LSBM Heat 2% (43.49%) higher than control (41.55%), respectively (p<0.05). Those experiments were designed to improve by-pass protein of diet and milk protein in the dairy cows. We will conduct those experiments the in vivo test by LSBM treatments in dairy cows diet.

충주 조정지댐 수문개도 영점조정 운영사례 연구

조상백(Sang-Baek Jo),장문성(Moon-Soung Chang),최교남(Kyo-Nam Choi),박채진(Chae-Jin Park),한철희(Cheol-Hee Han),강현구(Hyeon-Goo Kang) 대한전기학회 2014 대한전기학회 학술대회 논문집 Vol.2014 No.7

배추 부산물의 식이섬유를 첨가한 글루텐프리 튀김 프리믹스의 품질특성

해설여,박지현,허예나,김민주,배귀석,장문백,문보경,Hai, Xue-Ru,Park, Ji-Hyun,Heo, Ye-Na,Kim, Min-Joo,Bae, Gui-Seck,Chang, Moon-Baek,Moon, Bo-Kyung 한국식품조리과학회 2017 한국식품조리과학회지 Vol.33 No.2

Purpose: The purpose of this study was to determine the quality characteristics of gluten-free frying pre-mix with insoluble dietary fiber powder (IDFP) from Chinese cabbage by-product. Methods: Frying powder mix was prepared with 0.5, 1.0, 1.5 or 2.0% IDFP. Fried powder mix only whit brown rice powder was used as a control. Spreadability, pick-up ratio and color of batter, texture, moisture and oil contents, oil absorption and sensory evaluation of fried sweet potato were determined. Results: L and a-values of batter decreased with increasing amount of IDFP, whereas b-value increased with IDFP addition. Hardness and crispiness of fried sweet potato increased with IDFP addition until 1.5%. Moisture content of fried sweet potato increased with higher IDFP addition whereas oil content and oil absorption decreased with increasing amount of IDFP. Sensory evaluation of fried sweet potato showed the best results in the 0.5% of IDFP addition group. Conclusion: Overall preference of IDFP samples was significantly higher than that of control. Based on the study, the optimal amount of IDFP for gluten-free fried powder mix was determined to be 0.5-1.5%.

항균활성 천연물질을 이용한 반추위 메탄저감용 친환경 첨가제 개발

이아름,양진호,조상범,나종삼,심관섭,김영훈,배귀석,장문백,최빛나,신수진,최낙진,Lee, A-Leum,Yang, Jinho,Cho, Sang-Buem,Na, Chong-Sam,Shim, Kwan-Seob,Kim, Young-Hoon,Bae, Gui-Seck,Chang, Moon-Baek,Choi, Bitna,Shin, Su-Jin,Choi, Nag-Jin 한국유기농업학회 2014 韓國有機農業學會誌 Vol.22 No.3

본 연구는 천연물질에서 유래한 반추위 메탄저감용 친환경 첨가제 개발을 위해 각기 다른 종균을 이용하여 발효한 세신 추출물의 항균활성, 항산화활성 및 in vitro 반추위 발효시험을 체계적으로 실시하였다. 접종된 균주들의 성장효율을 알아보기 위해 실시한 생균수측정 결과 L. curvatus NJ40 균주에서 유의적으로 높은 균주성장을 나타냈다(p<0.05). 항균활성측정 결과는 대조구 대비 L. curvatus NJ40 및 L. plantarum NJ45 균주와 발효된 세신 추출물이 병원균에 대한 항균효과를 나타내는 것으로 조사되었다. In vitro 반추위 발효실험에 세신 및 발효 세신 추출물을 적용한 결과, 휘발성 지방산 생성량 대비 반추위 메탄 저감효과가 나타났다. 특히 반추위 미생물 활력 및 사료이용 효율을 대표할 수 있는 휘발성 지방산 생성효율에 대한 부정적 효과 없이, 오히려 휘발성지방산 생성효율을 향상시키면서 반추위 메탄 저감효과를 가져올 수 있는 것으로 나타났다. The present study was conducted to investigate effective starter culture to improve biological activity of Asarum sieboldii. Antibacterial activity, antioxidant activity and reduction of enteric rumen methane production were used as criterions for biological activity. Ground A. sieboldii was added in MRS broth at 10% (w/v) and fermented by different starter cultures. Weissella confusa NJ28, Weissella cibaria NJ33, Lactobacillus curvatus NJ40, Lactobacillus brevis NJ42, Lactobacillus plantarum NJ45 and Lactobacillus sakei NJ48 were used for starter culture strains. Each starter culture was inoculated with 1% (v/v) ratio and fermentation was performed at $30^{\circ}C$ with agitation (150 rpm) for 48 h. MRS broth for the control was employed without starter culture. Then the fermentation growth was dried and extracted using ethyl alcohol. The growth of starter culture was detected at NJ40, NJ42, NJ45 and NJ48. And the highest cell growth was found in NJ40. Antibacterial activity against to Staphylococcus aureus, Listeria monocytogens, Mannheimia haemolytica and Salmonella gallinarum were observed in the extract fermented by NJ40 and NJ45. All treatments showed antioxidant activities, however, there were no significant differences (p>0.05). In in vitro rumen fermentation, negative control (NC) and positive control (PC) were assigned to without extract and with non-fermented A. sieboldii extract. Significant suppression of gas productions were detected in positive control and treatments compared to negative control (p<0.05). However, total volatile fatty acid production was not suppressed. Significant methane reduction per total volatile fatty acid productions were found in positive control and NJ45 treatment (p<0.05). The present study suggested a fermentation of A. sieboldii using NJ45 strain could improve its biological activity and make possible for its use in bio additive for enteric rumen methane mitigation without suppression of animal productivity.

국내 참치 부산물 내 히스타민 생성 주요 세균의 특성 구명

방민우(Min-Woo Bang),정창대(Chang-Dae Chung),김선호,장문백(Moon-Baek Chang),이성실(Sung-Sil Lee),이상석(Sang-Suk Lee) 한국생명과학회 2009 생명과학회지 Vol.19 No.2

Biogenic amines은 발효제품과 같이 어류 속의 미생물에서 분비된 decarboxylase의 free amino acid의 decarboxylation에 의해 형성된다. 본 연구는 국내 어분 제조원료로 이용되고 있는 참치 부산물의 항영양인자로 작용하는 biogenic amines(histamine, tyramine, tryptamine, putrescine, cadaverine)의 분해조절을 위해 참치 부산물의 특성 구명을 위한 연구로 실시하였다. 참치 부산물의 pH 및 염도 농도는 6.51과 3.35% 이었으며, 참치 어분의 pH 및 염도 농도는 5.58과 5.83% 이었다. 참치 부산물 내 존재하고 있는 균주 및 주요 미생물을 확인한 결과 Total bacteria, aerobic plate count (APC), Total coliform (TC), Lactobacillus spp. 및 Bacillus spp.는 각각 9.20, 9.29, 5.67, 7.82 및 7.58(Log CFU/g)이었다. 참치 부산물 내히스타민 주요 생성균을 확인하기 위해 TLC 방법을 이용하였으며 히스타민 선택배지에서 추출한 미생물 중 총 7종의 히스타민 생성 균주를 선발하였다. 선발된 균주는 HPLC 분석을 통하여 히스타민 농도를 측정하여 아민 생성 미생물을 재확인하였다. Trypicase soy broth에 1% L-histidine (TSBH)을 첨가한 배지에서 분리한 7종의 히스타민 생성 균주를 16S rRNA 염기서열 분석 방법을 통해 분석한 결과, 참치 부산물내 주요 우점 아민 생성 미생물은 L. lactis subsp. lactis, K. pneummonlae, L. garvieae, V. olivaceus, H. alvei, L. garvieae 및 Morganella morganii가 주요 균주로 분석되었다. 16S rRNA 분석결과로 만들어진 phylogenetic tree는 다른 계통임을 보여주며 이는 biogenic amine을 생성하는 그람 양성 및 음성균으로 분류될 수 있다. Biogenic amines are generally formed through the decarboxylation of specific free amino acids by exogenous decarboxylases released by microbial species associated with the fish products and fermented feeds. This study was conducted to investigate the properties of e tuna waste regarding the control of degradation of biogenic amines (histamine, tyramine, tryptamine, putrescine, and cadaverine) that might be related with the anti-nutritional factor of the tuna waste that is used for manufacturing domestic fish meal. The values of pH and the salt content were 6.51, 3.35% in tuna waste and 5.58 and 5.83% in tuna fish meal, respectively. The strains and dominant bacteria tested in the tuna waste sample were 9.20, 9.29, 5.67, 7.82 and 7.58 log CFU/g of total bacteria, aerobic plate count (APC), total coliform (TC), Lactobacillus spp. and Bacillus spp., respectively. The main histamine forming-bacteria (HFB) in tuna waste were detected by silica gel thin-layer chromatography (TLC) and 7 histamine-forming bacterial species were isolated among microbes grown in selective medium. The histamine concentration was determined by detection of fluorescence of ο-phthaldialdehyde (OPA) derivatives using HPLC and the date were used to reconfirm the identities of the amine-producing bacteria. The 15 histamine-forming bacteria strains grown in trypicase soy broth (TSB) supplemented with 1% L-histidine (TSBH) were identified as Lactococcus(L.) lactis subsp. lactis, Klebsiella pneummonlae, L. garvieae 36, Vibrio olivaceus, Hafnia alvei and L. garvieae which were main dominant amine - producing strains, and Morganella morganii identified by 16S ribosomal RNA (rRNA) sequencing with PCR amplification. A Phylogenetic tree generated from the 16S rRNA sequencing data showed different phyletic lines that could be readily classified as biogenic amine forming gram-positive and negative bacteria.

대두박 대체 부산물 위주의 TMR 사료가 반추위 내 미생물의 In Vitro 발효특성에 미치는 영향

배귀석,김은중,송태호,송태화,박태일,최낙진,권찬호,장문백,Bae, Gui Seck,Kim, Eun Joong,Song, Tae Ho,Song, Tae Hwa,Park, Tae Il,Choi, Nag Jin,Kwon, Chan Ho,Chang, Moon Baek 한국초지조사료학회 2014 한국초지조사료학회지 Vol.34 No.2

본 연구는 농 식품부산물 중 비지박, 사자발약쑥 그리고 커피박의 유효미생물 (L. acidophilus ATCC 496, L. fermentum ATCC 1493, L. plantarum KCTC 1048, L. casei IFO 3533) 발효 사료원이 젖소 급여용 TMR (대조구) 중 대두박을 주로 대체한 각 비지박 (SC), 발효 비지박 (FSC), 발효 비지박+발효 사자발약쑥 부산물 (1:1, DM basis, FSCS) 3, 5, 10% 그리고 발효 비지박 + 발효 커피박 (1:1, DM basis, FSCC) 3, 5, 10%의 반추위 내 미생물 발효특성을 알아보기 위해 9처리구를 이용하여 3반복으로 in vitro 시험이 실시되었다. 배양 6~8시간대에 FSCS와 FSCC 처리구는 SC와 FSC 처리구에 비해 높은 pH 수준을 유지하였고, 대조구에 비해 낮은 pH 수준을 유지하였다 (p<0.05). 또한 배양 24시간대 FSC와 FSCC 3% 처리구는 가장 높은 pH를 유지하였다 (p<0.05). Gas 생성량은 배양 4~10시간대까지 FSCS와 FSCC 처리구가 대조구 보다 유의적으로 높았으나 SC와 FSC 처리구 보다 낮은 결과를 나타내었다 (p<0.05). 건물소화율은 배양 12시간대까지 FSC 처리구에서 유의적으로 높았고 (p<0.05), 24시간대에는 FSCS 10% 처리구가 가장 높은 결과를 나타내었다 (p<0.05). $NH_3-N$ 함량은 배양 4시간대까지 대조구에서 가장 낮았고 (p<0.05), 24시간에서는 FSC 처리구가 가장 높은 결과를 나타내었다 (p<0.05). 미생물단백질 합성량은 배양 시작 시 FSC, FSCS 그리고 FSCC 처리구가 4종의 Lactobacillus spp.에 의한 발효 때문에 대조구와 SC 처리구에 비해 높았으며 (p<0.05), 배양 10시간대 FSC 처리구 보다 SC, FSCS 그리고 FSCC 처리구에서 유의하게 높은 결과를 나타내었다 (p<0.05). Total VFA 농도는 배양 6~12 시간대에는 FSC 처리구에 유의하게 높았고(p<0.05), 배양 24시간대에는 FSCS 처리구에서 가장 높았다 (p<0.05). Acetate 농도는 배양 0~12 시간대에 FSC 처리구에서 가장 높았으며 (p<0.05), 24시간대에는 FSCS 5, 10% 처리구에서 가장 높은 결과를 나타내었다 (p<0.05). Propionate 농도는 FSC 처리구가 배양 0~10시간대까지 가장 높았으며 (p<0.05), 전체 배양기간 동안 FSCS 5, 10% 처리구와 FSCC 처리구에 비해 높은 경향을 나타내었다. Acetate/Propionate 비율은 배양 12시간대에 SC 처리구에서 가장 높았으며, 배양 24시간대에는 FSCS 3% 처리구가 가장 낮은 결과를 나타내었다 (p<0.05). $CH_4$ 가스 생성량은 FSC 처리구에서 배양 0~10시간대까지 가장 높았으며 (p<0.05), 24시간대에는 가장 낮은 결과를 나타내었다 (p<0.05). 배양 4~10시간대까지 FSCS와 FSCC 처리구는 SC와 FSC 처리구에 비해 유의하게 낮은 $CH_4$ 가스 생성량을 나타내었고 (p<0.05), 사자발약쑥과 커피박 함량이 증가할수록 $CH_4$ 가스 생성량은 배양 종료 시까지 낮은 경향을 나타내었다. 따라서, 본 in vitro 시험에서 젖소 급여용 TMR에 SC, FSC, FSCS 그리고 FSCC의 대체효과는 FSC 처리구에서 배양 초기부터 12시간대까지 반추위 내 미생물 발효 특성이 가장 높았다. FSCS와 FSCC 처리구는 배양 6~8시간대 부터 반추위 내 미생물 작용이 높아지는 특징을 나타내었으나, 배양 시작 후 6~8시간대까지 gas 생성량 및 $CH_4$ 가스 발생량은 낮아지는 특징을 나타내었다. 이와 같은 결론은 FSC는 젖소용 TMR 사료원 대체 효과가 높으며, TMR 사료원 중 FSCS와 FSCC 대체효과는 배양 초기 다소 낮은 반추위 내 미생물 발효 특성을 나타내었으나 $CH_4$ 저감효과를 나타내었다. 또한 배양 후기에는 SC와 FSC의 반추위 내 미생물 발효 특성과 비슷한 경향을 나타내어 TMR 사료원으로써 이용 가치가 높을 것으로 사료된다. This study was performed to evaluate the effects of replacing basic total mixed ration (TMR) with fermented soybean curd, Artemisia princeps Pampanini cv. Sajabal, and spent coffee grounds by-product on rumen microbial fermentation in vitro. Soybean in the basic TMR diet (control) was replaced by the following 9 treatments (3 replicates): maximum amounts of soybean curd (SC); fermented SC (FSC); 3, 5, and 10% FSC + fermented A. princeps Pampanini cv. Sajabal (1:1, DM basis, FSCS); and 3, 5, 10% FSC + fermented coffee meal (1:1, DM basis, FSCC) of soybean. FSC, FSCS, and FSCC were fermented using Lactobacillus acidophilus ATCC 496, Lactobacillus fermentum ATCC 1493, Lactobacillus plantarum KCTC 1048, and Lactobacillus casei IFO 3533. Replacing dairy cow TMR with FSC treatment led to a pH value of 6 after 8 h of incubation-the lowest value measured (p<0.05), and FSCS and FSCC treatments were higher than SC and FSC treatment after 6 h (p<0.05). Gas production was higher in response to 3% FSC and FSCC treatments than the control after 4-10 h. Dry matter digestibility was increased 0-12 h after FSC treatment (p<0.05) and was the highest after 24 h of 10% FSCS treatment. $NH_3-N$ concentration was the lowest after 24 h of FSC treatment (p<0.05). Microbial protein content increased in response to treatments that had been fermented by the Lactobacillus spp. compared to control and SC treatments (p<0.05). The total concentration of volatile fatty acids (VFAs) was increased after 6-12 h of FSC treatment (p<0.05), while the highest acetate proportion was observed 24 h after 5% and 10% FSCS treatments. The FSC of propionate proportion was increased for 0-10 h compared with among treatments (p<0.05). The highest acetate in the propionate ration was observed after 12 h of SC treatment and the lowest with FSCS 3% treatment after 24 h. Methane ($CH_4$) emulsion was lower with A. princeps Pampanini cv. Sajabal and spent coffee grounds treatments than with the control, SC, and FSC treatments. These experiments were designed to replace the by-products of dairy cow TMR with SC, FSC, FSCS, and FSCC to improve TMR quality. Condensed tannins contained in FSCS and FSCC treatments, which reduced $CH_4$ emulsion in vitro, decreased rumen microbial fermentation during the early incubation time. Therefore, future experiments are required to develop a rumen continuous culture system and an in vivo test to optimize the percentages of FSC, FSCS, and FSCC in the TMR diet of the dairy cows.

동물행동 및 복지 : 출산 후 새끼와의 분리에 따른 유우의 발성음 특성

김민진 ( Min Jin Kim ),손승훈 ( Seung Hun Son ),임신재 ( Shin Jae Rhim ),장문백 ( Moon Baek Chang ) 한국동물자원과학회 ( 구 한국축산학회 ) 2010 한국축산학회지 Vol.52 No.1

蛋白質 分解率과 에너지 水準이 反芻胃微生物 成長과 암모니아 態窒泰및 pH변화에 미치는 영향

張文伯 건국대학교 1988 論文集 Vol.26 No.1

This experiments were conducted to measure the rumen microbial growth, ammonia-N concentration and pH changes in the incubation media added with casein having different degradabilities(90.0, 76.5, 63.0, 49.5 and 36.0% )and different energy levels (low, medium and high). Casein treated with heat to reduce degradability and soluble carbohydrate was used as an energy source. The results obtained were as follows ; 1. Ammonia-N concentrations in the incubation media were average value of 1.98, 2.38 and 0.82mg/100ml, respectively in l7w, medium and high energy levels and the lowest value in the high energy level. 2. pH of the incubation media were average value of 6.27, 6.05 and 5.60 in the low, medium and high energy levels, respectively and the lowest pH were obtained in the highest energy level In the incubation mediums. However, casein degradability was not affected pH value. 3. Microbial cell density in the incubation media increased as increasing energy level and as increasing casein degradability.

Effect of Protein Treatment on Rumen Microbial Fermentation and Growth

Chang, Moon-Baek 中央大學校 食糧資源硏究所 1990 食糧資源硏究所 論文集 Vol.2 No.1

本 實驗은 反芻胃 內 세균과 원생동물에 의한 蛋白質源의 分解와 peptide 生成에 미치는 影響에 關하여 考察하고자 熱處理와 formaldehyde 處理의 影響을 누공이 付着된 緬羊으로 試驗하여 다음의 결과를 얻었다. (1) 緬羊 代謝試驗에서 無處理, 熱處理 그리고 formaldehyde 處理 카제인 添加 飼料區에 있어 공히 飼料給與 後 2時間째에 암모니아태 窒素濃度가 가장 높았으며 그 후 時間이 經過함에 따라 낮아지는 傾向을 나타내었다. 또한 無處理 카제인 添加 飼料를 給與한 緬羊의 芻反胃 內 암모니아태 窒素濃度가 熱處理나 formaldehyde 處理 카제인 添加 飼料를 給與할 때보다도 높았다. (2) 그러나 peptide 濃度는 蛋白質에 對한 處理方法이나 飼料給與後 時間經過에 關係없이 큰 差異를 나타내지 않았다. (3) 無處理 카제인 添加 飼料區에 있어 세균 純蛋白質 生成量은 원생동물 純蛋白質 生成量보다 약 3倍 정도 높았으나 熱處理 또는 formaldehyde 處理 카제인 添加 飼料 給與區에 있어서는 各各 平均 6倍와 4倍 정도 더 높은 傾向이였다. Effect of protein treatment on the rumen microbial growth and fermentation was studied with heat and formaldehyde on the bacterial and protozoal degradation of protein and peptide production were studied by fistulated sheep. The results obtained from these experiments are as follows. (1) The highest ammonia-N concentration in sheep fed untreated, heated and formal dehyde treated casein was 2 hour after feeding and decreased thereafter. Sheep fed untreated casein had the highest ammonia-N concentration in the rumen as compared with heat and formaldehyde treated casein. (2) However, peptide concentrations were not much different in all protein sources and time after feeding. (3) Bacterial true protein yield was almost 3 times higher than protozoal true protein yield with untreated casein, but it was average of 6 times and 4 times higher than protozoal true protein with heated and formaldehyde treated casein, respectively.

尖端科學技術을 應用한 反芻胃 醱酵調節

張文伯 中央大學校 遺傳工學硏究所 1990 遺傳工學硏究論集 Vol.3 No.1

At the year of 2000, GNP per capita year will exceed 5,000 dollar and a rapid increasas of animal product consumption will give rise to increase importation of feed grains which are heavily dependent upon from foreign supply. It is strongly suggested that maximum utilization of our own domestic feed resources and development of our own scientific technique on livestock management patterns are important. In this review paper, the various criteria for possible establishment of recombinant deoxyribonucleic acid systems in ruminal bacteria outlined and application for rumen additives to control to metabolism are discussed. Finally, application for Rusitec and/or computer modelling to manipulate feeding systems are suggested. Recombinant DNA technology offer a new approach to the study and eventual genetic manipulation of rumen becteria to modify the rate and extend of nutrient digestion within the rumen by controlled degradation of feedstuffs, regulation of specific fermentation products, control of specific bacterial species and stimulation of specific nutrient synthesis, but significant obstacles exist with this approach. Genetic systems of ruminal and of anaerobic bacteria of the mammalian gastrointestinal tract, in general, have not been studied and are largely unknown. Up to the present, the first and most successful antibiotic used as a feed additive for ruminant livestock belongs to a group of antibacterials termed ionophores. It is clear that there is a role for non-clinical antibiotics as feed additives for growth promotion and/or improvement in feed conversion efficiency in growing/fattening ruminants. Future research may yet show that antibiotics have a significant role to play in milk production. The most important features of Rusitec are the ability to maintain a steady fermentation pattern that is very similiar to the pattern observed in the rumen of the donor animals and expensive feed trials could be reduced. Quantitative or mathematical models for its data by computer simulation(modelling) are composed of equation representing the effcient feeding system of ruminants.