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초임계 유체 추출법을 이용한 용담사간탕(龍膽瀉肝湯) 약침액(藥鍼液)의 정유(精油)성분에 대한 연구(硏究)
이재은,김병우,임태진,김동희,권기록,Lee, Jae-Eun,Kim, Byeong-U,Im, Tae-Jin,Kim, Dong-Hui,Shin, Min-Seop,Yook, Tae-Han,Gwon, Gi-Rok 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.1
Objectives : This study was performed to analyze the effective components of essential oil obtained from Yongdamsagantang, which has been efficacious against leukorrhea in gynecologic diseases. Methods : I obtained the essential oils of Yongdamsagan-tang by hydrodistillation extraction method and supercritical fluid extraction(SFE) method, and then I analyzed those by GC/MS(Gas Chromatography/Mass Spectrum). Results : 1. The optimum SFE(Supercritical Fluid Extraction) condition was obtained in the following experiment conditions: pressure 200atm, temperature $45^{\circ}C$, duration of extraction 25minutes. 2. With GC(Gas Chromatography) and GC/MS(Gas Chromato- graphy/Mass Spectrum) analysis, I identified 37 compounds in the Yongdamsagan-tang's essential oil obtained through the SFE method. The main compounds were as follows : 3-Methyl-but-2-enoic acid,2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H -pyrano[3,2-g]chromen-3-yl ester(49.81%), (Z)-6-Pentadecen-1 -ol(3.19%), (-)-Spathulenol(2.40%). 3. I identified 4 compounds in the Yongdamsagan-tang's essential oil obtained through the hydrodistillation method. The main compounds were as follows : 3-Methyl-but-2-enoic acid, 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano [3,2-g]chromen-3-yl ester(2.61%). 4. 3-Methy I-but-2-enoic acid, 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano[3,2-g] chromen-3-yl ester, all were identified in both the SFE method and the hydrodistillation method, but the others were not identified in common. 5. I also conducted an additional test in order to examine the essential oil's antimicrobial action against bacteria. Both MIC(Minimum Inhibitory Concentrations) and MBC(Minimum Bactericidal Concentrations) were $0.125mg/m{\ell}$ against N. meningitidis, however MIC and MBC were $1.0mg/m{\ell}$ in antimicrobial action against 12 different genera of bacteria.
장재휘 ( Jang Jae Hwi ),강구정 ( Kang Gu Jeong ),김용훈 ( Kim Yong Hun ),임태진 ( Im Tae Jin ),강유나 ( Kang Yu Na ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<목적> 간섬유화과정에는 성상세포가 중요한 기능을 하는 것으로 알려져 있으며 성상세포를 포함한 간내 다른 세포에서 분비되는 TGF-β1이 간 섬유화 시작에 중요한 신호전달물질로 알려져 있다. 간 섬유화실험모델에서 가장 중요한 역할을 하는 것으로 알려진 성상세포가 섬유화과정에서 단계별로 활성화정도와 TGF-β1 발현관계를 일차적으로 알아보고자 했다. 아연(Zn)은 유리산소기(oxygen free radical) 포착제인 metallothionein (MT