기후변화에 따른 다목적댐 저수지의 취약성 평가

이혜숙,Lee, Hye-Suk 한국수자원학회 2012 물과 미래(한국수자원학회지) Vol.45 No.11

Synthesis and Evaluation of Coumaroyl Dipeptide Amide as Potential Whitening Agents

이혜숙,신경훈,Geun-Seog Ryu,조인식,김재일,Jae-Ho Lee,김한영 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.10

Coumaroyl dipeptide amide, Coumaric acid-LG-NH2, was prepared successfully using the solid-phase method, and its efficacy as a skin whitening agent was studied. Coumaric acid-LG-NH2 was prepared with Rink-amide resin, and 96.354% of purity was obtained. Using MTT assay and LDH release assay, we found that it exhibited very low cytotoxicity. And, we found that Coumaric acid-LG-NH2 inhibited tyrosinase activity dose-dependently and showed superior tyrosinase inhibitory activity to well-known whitening agent, arbutin. IC50 value of Coumaric acid-LG-NH2 was 182.4 μM, and IC50 value of arbutin was 384.6 μM. Also, in measurement of melanin contents using B16F1 melanoma cell lines, Coumaric acid-LG-NH2 reduced melanin production induced by α-MSH statistically significant, and showed superior melanin inhibitory activity to p-coumaric acid or arbutin. In addition, Coumaric acid-LG-NH2 reduced MC1R mRNA expression level. Thus, we concluded that MC1R pathway is the significant pathway of Coumaric acid-LG-NH2, and Coumaric acid-LG-NH2 has great potential to be used as novel whitening agents.

소박한 겉장을 열면 조촐한 생애가

이혜숙,Lee, Hye-Suk 대한출판문화협회 1996 출판저널 Vol.199 No.-

마취된 흰쥐 해마신경세포에서 Neurobiotin 이온주입으로 인한 신경세포의 생리적 특성의 변화

이혜숙,이만기,김영진,최병주,Lee, Hye-Sook,Lee, Maan-Gee-G.,Kim, Young-Jin,Choi, Byung-Ju 대한소아치과학회 1999 大韓小兒齒科學會誌 Vol.26 No.2

Pyramidal cells in the hippocampal CA area were recorded from and filled with neurobiotin in anesthetized rats. The extent of their dendrites and the electropharmacological properties of membrane as well as the effect before and after neurobiotin injection were examined. Pyramidal cells had a high resting membrane potential, a low input resistance, and a large amplitude action potential. A afterhyperpolarization was followed a single action potential. Most pyramidal cells did not display a spontaneous firing. Pyramidal cell displayed weak inward rectification and anodal break excitation in response to negative current injection into the cell. Membrane properties of recorded neurons before and after neurobiotin injection with consecutive current injection were compared. Some properties were significantly increased after labelling(P>0.05); the duration and amplitude of sustained AHP, input resistance, and the number of action potentials for simultaneous intra- and extracellular stimulations. Neurobiotin-filled neurons showed pyramidal morphology. Cells were generally bipolar dendrite processes ramifying in stratum lacunosum-moleculare, radiatum, and oriens. 마취된 흰쥐를 사용하여 해마의 CA영역에 위치한 피라밋세포들의 세포막 특성을 in vivo의 세포내 기록법에 의해서 관찰한 후 2.5% neurobiotin을 세포내 기록용 미세전극에 채워 세포내로 충진시킨후 충진전과 동일한 실험순서로 반응을 다시 관찰하고 ABC kit를 이용하여 면역조직염색을 행하여 형태학적인 관찰을 하였다. 피라밋세포의 세포내 반응 특성은 높은 휴지막 세포막전위, 낮은 input resistance 그리고 큰 활동전위를 가졌다. neurobiotin 충진 전 후에 따른 세포막 특성의 변화는 sustained AHP의 duration과 amplitude, input resistance, 그리고 세포외 및 세포내 자극에 따른 AP 수에서 유의한 차이를 보였고(P<0.05), 세포외 자극에 의한 억제는 주로 전반부에 나타났으며 CA3 영역에 위치한 이 세포의 형태학적 관찰 결과 세포체는 피라밋층에서 분명한 피라미드 형태를 띄고 있었고 기저 및 선단 가지가 각각 백색판층 및 섬유방-분자층까지 뻗어 있었으며 축삭은 겉질을 향해 기저가지돌기면에서 수직으로 뻗어 있었다. 해마의 주세포인 피라밋세포의 세포막 특성과 세포내 염색지시체(marker)로 주로 쓰이는 neurobiotin에 의해 세포막 특성중 일부가 변화됨을 알 수 있었고, 뇌내의 신경세포연결망이 완전히 보존되어 세포들 사이의 시냅스관계를 추측할 수 있는 in vivo 실험 모델이 응용될 수 있음을 제시하였다.

과기정론 - 첨단 연구장비 전문인력 육성 시급하다

이혜숙,Lee, Hye-Suk 한국과학기술단체총연합회 2012 과학과 기술 Vol.513 No.-

특집 제2차 과학기술기본계획-(3) 기초연구 진흥

이혜숙,Lee, Hye-Suk 한국과학기술단체총연합회 2008 과학과 기술 Vol.41 No.3

Safety effect of fermented oyster extract on the endocrine disruptor assay in vitro and in vivo

이혜숙,Hyun Hwang-Bo,Seon Yeong Ji,Seyeon Oh,Kyung-A Byun,Joung-Hyun Park,Bae-Jin Lee,Gi Young Kim,최영현 한국수산과학회 2021 Fisheries and Aquatic Sciences Vol.24 No.10

Oyster (Crassostrea gigas) is a marine bivalve mollusk widely distributed in coastal areas, and have been long widely used in industrial resources. Several studies demonstrated that fermented oyster (FO) extract attribute to bone health, but whether administration of FO play as an endocrine disruptor has not been studied. Therefore, in the present study, we investigated the effect of FO on the endocrine system in vitro and in vivo. As the results of the competitive estrogen receptor (ER) and androgen receptor (AR) binding affinities, FO was not combined with ER-α, ER-β, and AR. However, 17β-estradiol and testosterone, used as positive control, were interacted with ER and AR, respectively. Meanwhile, oral administration of 100 mg/kg and 200 mg/kg of FO doesn’t have any harmful effect on the body weight, androgen-dependent sex accessory organs, estrogen-dependent-sex accessory organs, kidney, and liver in immature rats. In addition, FO supplementation has no effect on the serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone, and 17β-estradiol. However, the relative weight of androgen- and estrogen-dependent organs were significantly increased by subcutaneously injection of 4.0 mg/kg of testosterone propionate (TP) and by orally administration of 1.0 μg of 17α-ethynyl estradiol (EE) in immature male and female rats, respectively. Furthermore, TP and EE administration markedly decreased the serum LH and FSH levels, which are similar those of mature Sprague-Dawley (SD) rat. Furthermore, the testosterone and 17β-estradiol levels were significantly enhanced in TP and EE-treated immature rats. Taken together, our findings showed that FO does not interact with ER and AR, suggesting consequentially FO does not play as a ligand for ER and AR. Furthermore, oral administration of FO did not act as an endocrine disruptor including androgenic activity, estrogenic activity, and abnormal levels of sex hormone, indicating FO may ensure the safety on endocrine system to develop dietary supplement for bone health.

큰 도전과 대단한 기회들

이혜숙,Lee, Hye-Suk 한국과학기술단체총연합회 2006 과학과 기술 Vol.39 No.4

1998년, 2008년, 2013년 제주지역 소아청소년의 흡입 알레르겐 감작률의 변화

이혜숙 ( Hye Sook Lee ),홍성철 ( Sung Chul Hong ),김정홍 ( Jeong Hong Kim ),김재왕 ( Jae Wang Kim ),이근화 ( Keun Hwa Lee ),이재천 ( Jae Chun Lee ) 대한보건협회 2014 대한보건연구 Vol.40 No.4

연구목적 : 본 연구는 1998년과 2008년의 후속연구로 동일지역에 거주하는 소아청소년의 흡입 알레르겐 감작률의 변화 양상을 조사하고자 하였다. 연구방법 : 2013년 11월 한 달간 제주지역 2개 초등학교, 1개 중학교, 2개 고등학교 학생 738명을 대상으로 흡입 알레르겐 16종에 대해 피부단자시험을 시행하였다. 연구결과 : 연구대상자의 연령은 7∼18세이며, 평균연령은 13.1±3.26세이었다. 피부단자시험에 유효한 689명에 대해 분석한 결과, 16종의 항원 중 1개 이상의 항원에 양성반응을 보인 경우는 59.2%이었으며, 연령이 증가함에 따라 감작률은 증가하는 경향을 보였다(p=.001). 감작률이 높은 흡입 알레르겐은 유럽집먼지진드기 38.0%, 북아메리카집먼지진드기 33.8%, 일본삼나무꽃가루 24.4%, 귤응애 23.8%, 긴털가루진드기 14.7% 순이었다. 주요 흡입 알레르겐 13종에 대한 감작률을 분석한 결과, 1998년 37.6%, 2008년 51.2%, 2013년 57.3% 로 감작률은 유의한 증가추세를 보였다(p=.017). 유의한 증가추세를 보인 흡입 알레르겐은 일본삼나무꽃가루, 귤응애, 바퀴벌레, 애완동물털류, 초기 개화 수목 꽃가루류 등이었으며, 두드러기쑥은 유의하게 감소하였다. 결론 : 제주지역 소아청소년의 흡입 알레르겐 감작률은 과거에 비해 증가추세가 뚜렷하였다. 추후에는 제주지역의 특이 알레르겐을 규명하는 노력과 함께 지속적이고도 정기적인 알레르겐 감작률의 변화양상에 대한 연구가 필요하다. Objective : This is a follow-up study of the surveys conducted in 1998 and 2008, to find change in the prevalence of sensitization to aeroallergen for children residing in the same communities. Methods : For this purpose, skin prick tests for 16 types of aeroallergen were applied to 738 students from 2 elementary schools, 1 middle school and 2 high schools. The tests were performed for one month, in November, 2013. The data were analyzed by a statistical program, “SPSS ver. 18.0(SPSS Inc., Chicago, IL, USA).” Results : Their age ranged from 7 to 18, with the average age of 13.1±3.26. The analysis of the 698 children effective for the skin prick tests showed the positive reaction to more than one among the 16 types of antigens in 59.2%. As for the sensitization rate, it showed increasing trends by their ageing (p=.001). The allergens of high sensitization rate were Dermatophagoides pteronyssinus (38.0%), Dermatophagoides farinae (33.8%), Japanese cedar (24.4%), Citrus red mite (23.8%), and Tyrophagus putrescentiae (14.7%). The sensitization rates in the 13 major aeroallergen showed a significantly increasing trend(p=.017): 37.6% (1998), 51.2% (2008), and 57.3% (2013). Aeroallergen with the significantly increasing trends in the sensitization rates included Japanese cedar, citrus red mites, cockroaches, animal hairs Ⅰ, and trees pollen mixture Ⅰ, etc. Conversely, ragweed showed a significantly decreasing trend. Conclusion : It can be concluded that the aeroallergen sensitization rate of the children in Jeju Province has been on the increase, compared to that of the past. Therefore, continuous and regular study of variation in sensitization rate to aeroallergen is required, with the further efforts to reveal the unique allergens in Jeju Province.

제주도의 도심, 감귤, 해안지역에 따른 초, 중, 고등학생의 흡입 알레르겐 감작률 비교연구

이혜숙 ( Hye Sook Lee ),홍성철 ( Sung Chul Hong ),김재왕 ( Jae Wang Kim ),김수영 ( Su Young Kim ),이근화 ( Keun Hwa Lee ),김정홍 ( Jeong Hong Kim ),최승효 ( Seung Hyo Choi ),임길채 ( Gil Chai Lim ),이재천 ( Jae Chun Lee ),김미옥 ( 대한보건협회 2011 대한보건연구 Vol.37 No.1

Objectives: This study is performed to find the difference of the regional inhalant allergy sensitization rates of the Students in Jeju Province, by comparing the urban, tangerine farming and coastal areas. Methods: The skin prick tests were applied to the 1,665 students of ages 9~16 in 3 schools in the urban area, 4 schools in the tangerine farming areas and 6 schools in the coastal areas. The skin prick tests were conducted from April 12~28, 2010. Results: There were 1,549 valid samples. The result showed 43.7% of the regional allergic sensitization rates of the Elementary, Middle and High School Students in Jeju Province. The sensitization rates by region were 50.2% in the tangerine farming areas, 40.9% in the urban areas, and 36.3% in the coastal areas. The allergens showing more than 7% sensitization rate included Dermatophagoides pteronyssinus, Dermatophagoides farinae, Japanese cedar, and Alternaria alternate. The tangerine farming areas showed 1.52 times higher sensitization rate to house dust mite, and 2.61 times to Japanese cedar while the coastal areas showed 3.45 times higher sensitization rate to Alternaria alternata. Conclusion: The sensitization rates showed a remarkable difference between the regions in jeju. The continuous and excessive exposure to allergens can develop or worsen allergic diseases. Therefore, counter measures are required to prevent and manage the regional allergens.