Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing

이신애,김현정,구본석,Seong Eun Lee,최자현,강예은 대한내분비학회 2022 Endocrinology and metabolism Vol.37 No.4

Complete genome sequence of Chryseobacterium sp. T16E-39, a plant growth-promoting and biocontrol bacterium, isolated from tomato (Solanum lycopersicum L.) root

이신애,김상윤,상미경,송재경,원항연,Lee, Shin Ae,Kim, Sang Yoon,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.4

토마토 뿌리에서 분리한 Chryseobacterium sp. T16E-39 균주는 식물생육촉진과 역병, 시들음병에 대한 억제효과가 있었다. 이 균주의 유전체 염기서열은 4,873,888 염기쌍이었으며 G + C 함량은 35.22%이었다. 이 유전체는 4,289개 단백질 유전자, 15개 rRNA 유전자, 71개 tRNA 유전자를 포함하였다. T16E-39 균주의 유전체에서 인산가용화, 식물호르몬 조절, 항산화 활성, 키틴 분해, 제9형 분비시스템에 관여하는 유전자를 확인하였으며, 이들 유전자는 식물의 생육을 촉진하고 병발생을 억제하는 기작과 관련되어 있을 것으로 판단된다. Chryseobacterium sp. strain T16E-39, isolated from roots of a tomato plant, promotes plant growth and suppresses phytophthora blight and bacterial wilt diseases. The complete genome of strain T16E-39 consists of a circular chromosome with 4,872,888 base pairs with a G + C content of 35.22%. The genome includes 4,289 coding sequences, 15 rRNAs, and 71 tRNAs. We detected genes involved in phosphate solubilization, phytohormone regulation, antioxidant activity, chitin degradation, and the type IX secretion system (T9SS) that may be related to growth promotion and disease suppression in plants.

토마토 뿌리에서 분리한 식물생육촉진 세균 Bacillus velezensis T20E-257균주의 유전체 염기서열

이신애,김상윤,상미경,송재경,원항연,Lee, Shin Ae,Kim, Sang Yoon,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon 한국미생물학회 2017 미생물학회지 Vol.53 No.4

Bacillus velezensis T20E-257 was isolated from the root tissue of a tomato plant and exhibited plant growth-promoting activity. Here we present the complete genome of strain T20E-257. The genome contains 3,900,066 base pairs with a G + C content of 46.7% in 2 contigs. The genome includes 3,708 coding sequences, 27 rRNAs, and 86 tRNAs. We found gene clusters encoding secondary metabolites with an antimicrobial activity and genes related to the production of indole-3-acetic acid and 2,3-butanediol, which play a role in plant growth and health. 토마토 뿌리에서 분리한 Bacillus velezensis T20E-257 균주는 식물촉진효과가 있었고, 본 연구에서 T20E-257 균주의 유전체 서열을 해독하였다. 유전체 초안에서 포함된 2개 contig는 총 염기서열이 3,900,066 bp고, G + C content가 46.7%이었다. 유전체에서 단백질 유전자 3,708개, rRNA 유전자 27개, tRNA 유전자 86개를 확인하였다. 항균활성을 가지는 2차 대사산물 생합성 관련 유전자군과 식물생육촉진에 관여하는 IAA와 2,3-butadiol 생합성 관련 유전자를 T20E- 257 균주 유전체에서 확인하였다.

Complete genome sequence of Variovorax sp. PMC12, a plant growth-promoting bacterium conferring multiple stress resistance in plants

이신애,김현수,김이슬,상미경,송재경,원항연,Lee, Shin Ae,Kim, Hyeon Su,Kim, Yiseul,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

본 연구에서 생물 및 비생물학적 스트레스에 내성을 유도하는 식물 생육 촉진 세균인 Variovarx sp. PMC12 균주의 유전체 염기서열을 분석하였다. PMC12 균주의 유전체는 5,873,297 bp와 1,141,940 bp 크기의 원형 염색체 2개로 구성되었다. 총 6,436개 단백질 유전자, rRNA 9개, tRNA 64개, ncRNA 3개와 유사유전자 80개가 확인되었다. 유전체상에서 발견된 1-aminocyclopropane-1-carboxylate (ACC) deaminase, 항산화 활성, 인산 가용화, 프롤린 생합성, 시드로포어 생합성과 관련된 유전자들은 PMC12 균주가 염, 온도, 병원균에 대한 스트레스에 대한 식물의 내성 유도와 관련되어 있을 것으로 판단된다. Variovorax sp. PMC12 is a rhizobacterium isolated from tomato rhizosphere and enhanced the plant resistance to abiotic and biotic stresses. Here we present the complete genome sequence of strain PMC12. The genome is comprised of two circular chromosomes harboring 5,873,297 bp and 1,141,940 bp, respectively. A total of 6,436 protein-coding genes, 9 rRNAs, 64 tRNAs, 3 ncRNAs, and 80 pseudogenes were identified. We found genes involved in 1-aminocyclopropane-1-carboxylate (ACC) deaminase, antioxidant activity, phosphate solubilization, and biosynthesis of proline and siderophore. Those genes may be related to capability of improving plant resistance to various stresses including salinity, cold temperature, and phytopathogen.

만삭임신후에 동반된 융모상피암

이춘배(Chun Bae Lee),장윤주(Youn Choo Jang),이진호(Jin Ho Lee),이정석(Jung Seok Lee),이지연(Ji Yeon Lee),이신애(Shin Ae Lee),김종우(Jong Woo Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.7

Gestational trophoblastic tumor became one of the curable disease due to the development of chemotherapy, appropriate follow up of β- human chorionic gonadotropin and others. But metastatic trophoblastic tumor still shows high mortality rate because of resistance to the chemotherapy and large tumor burden.One of the important prognostic factors determining the prognosis of gestational trophoblastic tumor is antecedent pregnancy, which affects the response to therapy. Choriocarcinoma following term pregnancy is rare one with the incidence of one per 160,000 pregnancies in United States and the prognosis is poor because of late diagnosis and combined high risk prognostic factors.We present a choriocarcinoma following term pregnancy that required emergency total abdominal hysterectomy due to profuse bleeding with brief review of literatures.

컴퓨터 보조 진단 시스템 성능 개선을 위한 새로운 이미지 증대 기법

이신애(Sin-ae Lee),조현종(Hyun-chong Cho) 대한전기학회 2021 전기학회논문지 Vol.70 No.1

Gastric cancer is the largest percentage of cancer cases in Korea. A precise way to find the occurrence of gastrointestinal diseases is through gastroscopy by a trained diagnostic physician. Computer-aided diagnosis (CADx) system helps improve the reliability and speed of diagnosis. The CADx system has developed with deep learning, which is data dependent. However, medical image data is labor intensive and time consuming, making it difficult for large data sets to be formed. To solve this problem, it is important to apply augmentation techniques. In this paper, we propose an augmentation method which is suitable for the data. A basic classification model was made by leaning a data set consisting of the original images. Each of the 14 augment technique-applied data set was input into the generated model and the f1-score values were compared. The f1-score of the highest performance among the proposed methods, was 0.9221, with an increase of about 0.085.

시상하부 과오종과 연관된 성조숙증을 동반한 신경섬유종증 1예

이신애(Shin Ae Lee),김진호(Jin Ho Kim),김선준(Sun Jun Kim) 대한소아신경학회 2018 대한소아신경학회지 Vol.26 No.1

신경섬유종증 1형은 흔히 보일 수 있는 신경피부증후군의 하나로, 담갈색 반점, 겨드랑이 주근깨 모양 색소반, 피부신경섬유종 그리고 Lisch 소결절 등을 특징적으로 나타내며, Neurofibromin을 발현하는 NF1 유전자의 변이가 이 질환의 원인으로 알려져있다. NF1 환아에게 있어서 성조숙증을 같이 동반하는 경우가 있으며, 이런 경우 대부분 시신경 종양과 연관되어있다. 저자들은 NF1에서 시상하부 과오종과 연관된 성조숙증을 동반한 케이스를 보고하고자 하며, 이 환아의 경우 지금까지 보고되지 않았던 NF1 exon 5의 14-bp 결실 돌연변이를 동반하고 있는 바이다. Neurofibromatosis type 1 (NF1) is a common neurocutaneous syndrome that presents with multiple café-au-lait spots, skinfold freckling, dermatofibromas, neurofibromas, and Lisch nodules. Mutations of the NF1 gene, encoding the protein neurofibromin, have been identified as the cause of this disease. NF1 can also present with precocious puberty and be associated with optic pathway tumors. Hypothalamic hamartoma as the cause of precocious puberty in patients with NF1 has been rarely described in the literature. Here, we report the findings for a patient with NF1 and precocious puberty associated with a hypothalamic hamartoma who had a newly discovered 14-bp deletion mutation in exon 5 of NF1. To our knowledge, this is the first time this combination is reported in the literature.

간호대학생이 지각한 임상실습현장지도자의 돌봄에 대한 한국어판 측정도구의 타당도와 신뢰도 분석

이신애(Shinae Lee),박효정(Hyojung Park) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.12

본 연구는 국내 간호대학생을 대상으로 Wade 와 Kasper가 개발한 임상실습현장지도자의 돌봄에 대한 측정도구의 타당도와 신뢰도를 검증하기 위하여 시행되었다. D 광역시 3개 대학교 간호학과 3∼4학년에 재학 중인 간호대학생 219명을 대상으로 2018년 6월 04일에서 6월 20일까지 자료 수집을 하였다. 자료 분석은 SPSS 21.0과 AMOS 21.0 프로그램을 활용하여 타당도와 신뢰도 검증을 실시하였다. varimax 직각 회전을 이용한 탐색적 요인분석을 시행한 결과, 총 27문항 5개 확인되었으며 존중하는 배려, 돌봄을 통한 신뢰, 통제와 유연성, 지지적인 교육환경, 삶의 의미에 대한 공감으로 구분되었다. 동시타당도 검정 결과, 임상실습교육환경 측정도구의 상관관계에서 r=.64 (p<.001)로 나타나 타당도가 있는 도구임이 확인되었다. 최종 도구의 Cronbach’s α는 .88이었고, 각 요인별 Cronbach’s α는 .91, .86, .80, .76, .85로 나타나 신뢰도가 높음을 확인하였다. 본 연구에서 검증된 한국어판 NSPIC 도구는 임상실습현장지도자의 돌봄 역량을 객관적으로 평가하기에 유용한 도구로서 적용가능성을 제시하였다는 것에 의의가 있다. 또한 본 연구는 임상실습현장지도자의 돌봄 역량을 향상시키기 위한 프로그램을 개발하는 데 도움을 줄 것으로 기대한다. The purpose of this study was to examine the validity and reliability of the Korean version of the nursing students" perception of instructor caring (NSPIC) scale developed by Wade and Kasper for nursing students. We collected data from 219 nursing students in the 3rd to 4th year at three nursing colleges from June 4 to June 20, 2018. Data were analyzed using SPSS 21.0 and AMOS 21.0. An exploratory factor analysis with varimax rotation was performed, resulting in five factors (respectful sharing, confidence through caring, control versus flexibility, supportive learning climate, appreciation of life"s meanings) with a total of 27 items. Confirmatory factor analysis supported good convergent and discriminant validities. In addition, the concurrent validity test confirmed that the K-NSPIC scale was a validity tool as the correlation of the clinical learning environment (CLE) scale appeared as r=.64 (p<.001). The Cronbach"s alpha coefficient of the K-NSPIC was .88, and Cronbach"s alpha coefficient for each of the five factors was .91, .86, .80, .76, and .85; internal consistency was confirmed. It is significant that the K-NSPIC proved applicable as a useful tool for assessing instructor caring. It is also expected that it will assist in the design of programs to improve the caring ability of instructors.

SHRIMP U-Pb 저어콘 연대측정 결과처리법

이신애(Shinae Lee),이기욱(Keewook Yi) 대한지질학회 2015 대한지질학회 학술대회 Vol.2015 No.10

수업 준비를 위한 초등 과학 교사용 지도서 활용 실태 -2009 개정 과학과 3-4학년을 중심으로-

이신애 ( Shin Ae Lee ),임희준 ( Hee Jun Lim ) 한국초등과학교육학회 2016 초등과학교육 Vol.35 No.2

This study aims to investigate how elementary teachers use teachers`` guide in preparation for science lessons. First, different uses of teacher``s guide were analyzed. Second, how and why teachers use each section in teacher``s guide were analyzed. For the study, 24 elementary school teachers were interviewed in depth. The semi-structured in-depth interviews were conducted individually and/or in small group, and additional interviews were held when necessary. The results showed that most of the teachers used teacher’s guide only substitutionally, and some teachers rarely use teacher``s guide, while only 3 out of 24 teachers used teacher’s guide in detail. The reasons that teachers used the teacher’s guide substitutionally or rarely were that most science lessons include experiments, and science textbook itself provides enough information for preparation of science lessons for 3rd and 4th grade students. The results also revealed that only few teachers read the general guideline in teacher``s guide. Some sections of teacher``s guide were not used. The sections that many teachers used were the aims of lesson, the learning system of the unit, background knowledge, flow of lesson, learning contents and activities. This study specifically examined the actual use of teacher’s guide for lesson preparation and discussed implications for the development of more helpful teacher``s guide.