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Leucobacter denitrificans sp. nov., Isolated from Cow Dung
원항연,Rangasamy Anandham,Tomohiko Tamura,Moriyuki Hamada,김수진,김이슬,Ken-ichiro Suzuki,권순우 한국미생물학회 2012 The journal of microbiology Vol.50 No.1
The bacterial strain M1T8B10T was isolated from cow dung in Suwon, Republic of Korea. The strain was a Gram stainpositive rod, nonmotile, and non-spore-forming. According to 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter, showing the highest sequence similarities with Leucobacter aridicollis L-9T (98.7%), Leucobacter iarius 40T (98.4%), and Leucobacter komagatae JCM 9414T (98.2%). Cell-wall peptidoglycan contained the diagnostic diamino acid 2,4-diaminobutyric acid of the genus Leucobacter, showing B-type cross-linked peptidoglycans. The major fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. The quinone system consisted of the menaquinones MK-11 (78%) and MK-10 (22%). The polar lipid profiles contained diphosphatidylglycerol, phosphatidylglycerol, and an unidentified glycolipid. Differences in several physiological features including nitrate reduction enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic, chemotaxonomic, and phenotypic results, the isolate represents a novel species, for which the name Leucobacter denitrificans sp. nov. is proposed. The type strain is M1T8B10T (=KACC 14055T =NBRC 106309T).
원항연,김병용,허온숙,박인철,서주원,송재경 한국미생물학회 2011 The journal of microbiology Vol.49 No.5
Scab disease significantly damages potatoes and other root crops. Some Streptomyces species have been reported as potato-scab pathogens. Identification of the phytopathogenic Streptomyces is mainly done on the basis of the 16S rRNA gene, but use of this gene has some limitations for discriminating these strains because they share high similarities of 16S rRNA gene sequences. We tested the RNase P RNA (rnpB) gene as a taxonomic marker to clarify the relationship among closely related scab-causing Streptomyces strains. The rnpB genes were analyzed for 41 strains including 9 isolates from Jeju, Korea. There were 4 highly variable regions including nucleotide gaps in the rnpB genes. Interspecies similarity of the rnpB gene in tested Streptomyces strains was lower than about 97%, while the intraspecies similarity was higher than about 98%. Phylogenetic analysis demonstrated that the rnpB tree has similar topology to the 16S rRNA gene tree, but produces a more divergent phyletic lineage. These results revealed that the rnpB gene could be used as a powerful taxonomic tool for rapid differentiation of closely related Streptomyces species. In addition, it was also suggested that the variable regions marked as α, β, γ, and δ in the rnpB gene could be useful markers for the detection of specific Streptomyces species.
Characterizing Bacterial Communities of Paddy Soils in Korea
Hang-Yeon Weon(원항연),Jae-Ho Joa(좌재호),Won-Il Choi(최원일),Byung-Koo Ahn(안병구),Young-Han Lee(이영한),An-Sung Roh(노안성),Hee-Kwon Kim(김희권),Seung-Chul Choi(최승출),Moon-Tae Choi(최문태),Jun-Hong Park(박준홍),Jae-Hyung Ahn(안 한국토양비료학회 2012 한국토양비료학회 학술발표회 초록집 Vol.2012 No.6
Bacillus amyloliquefaciens GR4-5 균주의 토양 내 정량 분석
김다연,김병용,안재형,원항연,김성일,김완규,송재경 한국유기농업학회 2015 韓國有機農業學會誌 Vol.23 No.4
Bacillus amyloliquefaciens GR4-5 was isolated from the rhizosphere soil of Korean ginseng and displayed broad-spectrum suppression of ginseng root rot pathogens. The survivability of B. amyloliquefaciens GR4-5 in soil was investigated under three different conditions; indoor, outdoor ─ of which soil was put in 14 mL tube after treatment ─ and field environments. Soil samples were collected over a fourweek period from three experimental designs, and assessed for 16S rRNA gene copy number by quantitative polymerase chain reaction (qPCR). In outdoor condition, the 16S rRNA gene copy number of Bacillus spp. was 8.35 log copies g soil-1 immediately after the GR4-5 treatment. Two weeks later, the 16S rRNA gene copy number of Bacillus spp. (6.70 log copies g soil-1) was similar to that of the control (6.38 log copies g soil-1). In indoor condition, the 16S rRNA gene copy number of Bacillus spp. maintained in a certain level for a longer period than those in outdoor and field. The 16S rRNA gene copy number of Bacillus spp. in field experiment was reduced faster than that of outdoor condition. Our results show that B. amyloliquefaciens GR4-5 can survive in bulk soil for 1 week, indicating its potential use as a biocontrol agent following 7 day application intervals. This study presents that outdoor microcosm system design could be a useful method to assess easily the survivability of beneficial microorganisms.
좌재호,원항연,현해남,전용철,고상욱 한국미생물학회 2014 The journal of microbiology Vol.52 No.12
This study was conducted to assess bacterial species richness,diversity and community distribution according to differentfertilization regimes for 16 years in citrus orchard soil of volcanicash. Soil samples were collected and analyzed fromCompost (cattle manure, 2,000 kg/10a), 1/2 NPK+compost(14-20-14+2,000 kg/10a), NPK+compost (28-40-28+2,000kg/10a), NPK (28-40-28 kg/10a), 3 NPK (84-120-84 kg/10a),and Control (no fertilization) plot which have been managedin the same manners with compost and different amount ofchemical fertilization. The range of pyrosequencing readsand OTUs were 4,687–7,330 and 1,790–3,695, respectively. Species richness estimates such as Ace, Chao1, and Shannonindex were higher in 1/2 NPK+compost than other treatments,which were 15,202, 9,112, 7.7, respectively. Dominantbacterial groups at level of phylum were Proteobacteria, Acidobacteria,and Actinobacteria. Those were occupied at 70.9%in 1/2 NPK+compost. Dominant bacterial groups at levelof genus were Pseudolabrys, Bradyrhizobium, and Acidobacteria. Those were distributed at 14.4% of a total of bacteriain Compost. Soil pH displayed significantly closely relatedto bacterial species richness estimates such as Ace, Chao1(p<0.05) and Shannon index (p<0.01). However, it showedthe negative correlation with exchangeable aluminum contents(p<0.05). In conclusion, diversity of bacterial communityin citrus orchard soil was affected by fertilization management,soil pH changes and characteristics of volcanic ash.