Comparison of Efficiency of Self-renewal and Differentiation Potential in Tendon-derived Mesenchymal Stem Cells Isolated by Magnetic-activated Cell Sorting Method or Colony Picking Method

이모세,최유림,윤동석,이진우,윤길성,최우진,한승환,Lee, Moses,Choi, Yoorim,Yoon, Dong Suk,Lee, Jin Woo,Yoon, Gil Sung,Choi, Woo Jin,Han, Seung Hwan The Korean Foot and Ankle Society 2014 대한족부족관절학회지 Vol.18 No.3

Purpose: The purpose of this study is to evaluate the efficacy of mesenchymal stem cell (MSC) isolation by the magnetic-activated cell sorting (MACS) method in tendon tissue-derived cells compared to the colony picking method for isolation of MSCs by picking colony-forming cells. Materials and Methods: Human tendon-derived cells were isolated by enzyme digestion using normal tendon tissues from three donors. We used the magnetic kit and well-known MSC markers (CD90 or CD105) to isolate MSCs in tendon-derived cells using MACS. Cloning cylinders were used to isolate colony-forming cells having MSC characteristics in tendon-derived cells. Colony-forming unit-fibroblast (CFU-F) assay was used to evaluate the self-renewal capacity of cells isolated using the colony picking method or MACS. For comparison of differentiation potentials into osteogenic or adipogenic lineage between two groups, alizarin red S and oil red O staining were performed at 14 days after induction of differentiation in vitro. Results: Flow cytometry results showed that early passage tendon-derived cells expressed CD44 in 99.13%, CD90 in 56.51%, and CD105 in 86.19%. In the CFU-F assay, CD90+ or CD105+ cells isolated with MACS showed larger colony formation in size than cells isolated using the colony picking method. We also observed that CD90+ or CD105+ cells were constantly differentiated into both osteogenic and adipogenic lineages in cells from all donors, whereas cells isolated using the colony picking method were heterogeneous in differentiation potentials to the osteogenic and adipogenic lineages. Conclusion: CD90+ or CD105+ cells isolated using MACS showed superior MSC characteristics in the self-renewal and multi-differentiation capacities compared with cells isolated using the colony picking method.

Outcomes of Diffuse-Type Pigmented Villonodular Synovitis (PVNS) after Open Total Synovectomy

이모세,이수현,서진석,양우익,신규호,Lee, Mo-Ses,Lee, Soo-Hyun,Suh, Jin-Suck,Yang, Woo-Ik,Shin, Kyoo-Ho The Korean Musculoskeletal Tumor Society 2010 대한골관절종양학회지 Vol.16 No.1

목적: 색소 융모 결절성 활액막염은 슬관절을 비롯한 주요 관절에서 드물게 발생하는 연부 조직 종양으로서 수술 적 치료 후 재발 빈도가 높은 것으로 알려져 있다. 본 연구에서는 주요 관절에 발생한 미만형 색소 융모 결절성 활액막염에 대한 활막 전 절제술 후 임상 경과를 보고하고, 기존에 발표 된 문헌과 비교 분석하고자 한다. 대상 및 방법: 1994년 2월부터 2006년 3월까지 미만형 색소 융모 결절성 활액막염으로 치료 받은 21예의 환자를 대상으로 하였으며, 발생 부위는 슬관절 14예, 족관절 5예, 견주관절 2예가 있었다. 평균 추시 기간은 5년 5개월이었다(36-157개월). 발생 연령은 평균 34세였으며 남자가 7명, 여자가 14명이었다. 후향적 연구 방법으로 관절 운동 범위 측정을 포함한 정기적인 임상 양상 평가를 하였다. 결과: 총 21예의 미만형 환자 중 1예를 제외 한 전례에서 활막 전 절제술 및 전기소작술을 통한 보강 치료를 시행하였으며, 정기적인 외래경과 관찰 상 재발이 의심되었던 2예에서 재수술 후, 1예에서만 조직학적 재발이 확진 되었다. 활막 전 절제술을 시행하지 못한 1예와, 재발로 재수술 받은 1예에서 술 후 방사선 치료를 시행 하였다. 임상 기능 평가상 술전과 비교하여 모든 부분에서 통계적으로 유의한 호전이 있었다.(p<0.0001). 슬관절에 발생한 병변으로 수술적 치료를 시행 후 관절 운동 장애로 제약이 있던 환자는 2예(총 14예) 있었다. 결론: 최소 3년 이상의 추시 기간 동안 활막 전 절제술 및 전기 소작술을 통한 보강 치료로 호전된 임상 결과 및 낮은 재발율의 치료 효과를 얻을 수 있었다. 잦은 재발과 주요 관절에 발생하여 국소적 침투, 파괴로 기능 장애를 일으키는 미만형 색소 융모 결절성 활액막염에 치료에 있어 적절한 활막 전 절제술이 시행 될 경우 방사선 보강 치료는 제한 된 경우에만 필요할 것이다. Purpose: Pigmented villonodular synovitis (PVNS) is a rare soft tissue tumor, which usually arises in larger joints, such as the knee. It has a high recurrence rate after surgical treatment. The purpose of this study is to evaluate and analyze the clinical results of diffuse-type pigmented villonodular synovitis cases that were treated with open total synovectomy. Materials and Methods: Between 1994 and 2006, 21 patients who had diffuse-type pigmented villonodular synovitis were selectively reviewed. Among the 21 cases studied, 14 patients presented at the knee, 5 at the ankle, and 2 at the shoulder and elbow. The mean follow up period was 5.5 years (range, 36-157 months). The average age of the patients was 34 years consist of 7 men and 14 women. Clinical outcomes were analyzed retrospectively, including range of motion and complications. Results: Open total synovectomy and adjuvant electrocautrization were done in all cases except one. During the regular follow-up period after the surgery, two patients showed symptoms of recurrence. After re-operation, only one case was pathologically confirmed as a recurrence. The patient who had partial synovectomy and the other patient who had second operation due to recur rence received additional radiation therapy. Clinical outcome scores were improved in every aspect (p<0.0001). 2 out of 14 Patients who had pigmented villonodular synovitis at the knee developed stiff knee after the surgery. Conclusion: After the open total synovectomy with electrocautrization, a low recurrence rate and satisfactory clinical outcome was achieved, observed in a minimum of 3 years of follow-up.

SRGAN을 활용한 Sentinel-2 영상의 초해상화와 GEDI LiDAR를 통한 캐노피 추정

이모세(Lee, Mose),양병윤(Yang, Byungyun) 한국측량학회 2023 한국측량학회지 Vol.41 No.6

기후변화로 인한 산림 생태계의 변화 파악이 중요해지면서 지상 바이오매스를 추정하는 연구가 꾸준히 수행됐다. 지상 바이오매스를 추정하는 연구는 현장에서 직접 측량하거나 항공 및 위성영상을 활용하는 방식을 널리 사용했다. 이 방법들은 높은 비용, 인력문제, 자료 취득의 문제, 연구지역 범위 설정 등의 문제가 있어 무료로 제공되는 위성영상 자료의 활용이 불가피하게 됐다. 따라서 본 연구에서는 SRGAN을 활용한 Sentinel-2 이미지의 초해상화(Super-Resolution)와 GEDI (Global Ecosystem Dynamics Investigation) LiDAR를 활용하여 산림 캐노피의 높이를 추정하려는 목표를 가진다. 구체적으로 초해상화 과정은 GAN기반 네트워크인 SRGAN을 활용해 10m의 공간해상도를 가진 Sentinel-2 이미지를 2.5m로 초해상화 하였다. 이를 통해 개선된 Sentinel-2 이미지를 활용해 산림의 변화탐지 가능성을 파악했다. 다음으로 GEDI 데이터로부터 캐노피 높이 값을 추출하여 일반 크리깅과 역거리가중법을 통해 미측정 지역에 대한 보간을 수행하여 넓은 지역에 대한 산림 캐노피 높이를 추정했다. 마지막으로 산림 캐노피의 평균값 및 분포를 분석하고, GEDI와 Sentinel-2 데이터를 활용하여 보다 정밀한 산림 생태계 파악을 가능하게 하였다. 따라서 본 연구를 통해 광범위한 지역을 커버할 수 있는 비용 효율적인 방법의 산림 생태계 모니터링 방식을 제안하여 산림 관리와 보존에 있어 지속 가능한 산림 관리에 기여할 것으로 기대한다. The increasing importance of understanding the changes in forest ecosystems due to climate change has led to consistent research in estimating AGB (Above-Ground Biomass), which typically involves surveying or the use of aerial and satellite imagery. These methods, however, face challenges related to cost, labor, and determining the size of research areas. For the reason, it is unavoidably relying on freely available satellite imagery data. Thus, this study aims to use SRGAN (Super-Resolution Generative Adversarial Network ) for enhancing the resolution of Sentinel-2 images and to estimate forest canopy height using GEDI (Global Ecosystem Dynamics Investigation) LiDAR. Specifically, the super-resolution process employed SRGAN to enhance the 10m spatial resolution of Sentinel-2 images to 2.5m, improving the ability to detect changes in forests using Sentinel-2 images. Furthermore, canopy height values from GEDI data were interpolated for unmeasured areas using OK (Ordinary Kriging) and IDW (Inverse Distance Weighting), allowing for the estimation of forest canopy height over a large area. Finally, this study analyzed the average values and distribution of the forest canopy, and utilized both GEDI and Sentinel-2 data for a more precise understanding of the forest ecosystem. Therefore, this research proposes a cost-effective method for extensive forest ecosystem monitoring, contributing to sustainable forest management and conservation.

실시간 분석을 위한 도커 컨테이너 기반의 딥러닝 모델 관리 시스템 설계 및 성능 비교

이모세(Mo-se Lee),강민수(Min-su Kang),김인호(In-ho Kim),김재헌(Jae-hun Kim) 한국통신학회 2021 韓國通信學會論文誌 Vol.46 No.2

스마트시티를 위한 실시간 데이터 처리 아키텍처

이모세(Mo-se Lee),강민수(Min-su Kang),김홍준(Hong-joon Kim),김재헌(Jae-hun Kim) 한국통신학회 2021 韓國通信學會論文誌 Vol.46 No.2

구형 유도핀 (Bulb-tipped Guide Wire)과 K 강선을 이용한 파손된 금속정 제거술

이모세 ( Mo Ses Lee ),양규현 ( Kyu Hyun Yang ) 대한골절학회 2010 대한골절학회지 Vol.23 No.4

To report the unusual failure of proximal femoral nail antirotation (PFNA) at the level of lag screw hole and introduce a simple technique for removal of a broken intramedullary nail with a narrow hollow using a bulb-tipped guide wire and Kirschner wire.

인공발목관절의 임상시험 가이드라인 개발을 위한 문헌적 고찰

박진오,이모세,이진우,이수빈,한승환,Park, Jin Oh,Lee, Moses,Lee, Jin Woo,Lee, Soo Bin,Han, Seung Hwan 대한족부족관절학회 2014 대한족부족관절학회지 Vol.18 No.4

Purpose: The purpose of this study is to develop guidelines for clinical trial of the total ankle replacement system for premarket approval. Materials and Methods: We selected and analyzed nine peer-reviewed articles whose quality had been proven in a previous phase. Two investigators extracted parameters for guideline criteria, including number of cases, patient age, follow-up period, failure rate, radiographic osteolysis rate, residual pain rate, and percentage of satisfaction. In addition, the inclusion and exclusion criteria were analyzed and developed. Results: Eight level IV studies and one level II study were included. The average number of cases was 159 cases and the mean patient age was 63.5 years. The mean follow-up period was 4.2 years, ranging from two to nine. The average failure rate of total ankle replacement in mid- to long-term follow-up was approximately 13% (2%~32.3%). The rate of osteolysis was approximately 18%. Residual pain was common (21.4%~46%), but overall patient satisfaction was approximately 85.6% (67.5%~97%). Conclusion: The results could be used as criteria for designing the clinical studies, such as number of cases, patient age (over 60 years), and follow-up period (minimum two years). The clinical scoring system and 36-item short form health survey (SF-36) was the most commonly used method for clinical evaluation for total ankle arthroplasty. In addition, the overall results, including failure rate, osteolysis rate, and patient satisfaction, could be used as a parameter of guidelines for premarket approval.

비스포스포네이트 제제 관련 관절통 및 근육통에 대한 지역 약국 약사 및 환자 능동적 모니터링

송정우,박소희,윤동원,이모세,이정민,김나영,김영욱,이주연,신주영,Song, Jung-woo,Park, So-Hee,Yoon, Dongwon,Lee, Mo-Se,Lee, Jung-Min,Kim, Na-Young,Kim, Young-Wook,Lee, Ju-Yeun,Shin, Ju-Young 한국임상약학회 2021 한국임상약학회지 Vol.31 No.3

Objective: We investigated arthralgia and myalgia associated with the use of oral bisphosphonate (BP) by conducting a survey of patient. Methods: The pharmacists conducted a survey between 1 Oct 2019 and 30 Sep 2020 among patients who were dispensed BP in community pharmacies to assess their demographic and medical characteristics, and their experiences with, and process for pain. Logistic regression analyses were performed to find the risk factors associated with the pain, and the odds ratios (OR) and 95% confidence intervals (CI) were determined. Results: A total of 160 patients who used BP participated in the survey (74 [46.3%] used risedronate; 61 [38.1%] used alendronate; 23 [14.4%] used ibandronate), and 20 (12.5%) of them experienced pain. Significant statistical differences of the characteristics between patients who experienced pain or not were observed regarding menopause, and parity. Compared with women who had one or two parity, women who had more than three parity were associated with the decreased risk of pain (OR, 0.24; 95% CI, 0.04-0.98). Moreover, steady exercise was associated with the decreased risk of pain compared to less exercise (OR, 0.37; 95% CI, 0.14-0.98). Conclusion: Our findings suggest that the risk of pain in BP-treated patients might be different regarding the different ingredients of BP, and dosing frequency. This survey highlights a need for a further safety research to understand the factors influencing the pain associated with the BP treatment.

연골 모세포종 - 골단과 견인골단의 비교 -

이승환,이모세,신규호,한수봉,Lee, Seung-Hwan,Lee, Mo-Ses,Shin, Kyoo-Ho,Hahn, Soo-Bong 대한근골격종양학회 2008 대한골관절종양학회지 Vol.14 No.1

목적: 연골 모세포종은 드문 원발성 양성 골종양으로 대부분이 장관골의 골단에 호발하는 것으로 알려져 있다. 본 연구에서는 19례의 환자를 대상으로 골단과 견인골단에 발생한 연골모세포종을 비교 분석하고자 하였다. 대상 및 방법: 1987년 8월부터 2005년 8월까지 연골모세포종으로 치료 받은 19례의 환자를 대상으로 하였으며 평균 추시기간은 5년이었다. 발생 연령은 평균 17.6세였으며 남자가 15명, 여자가 4명이었다. 수술적 치료로는 소파술, 소파술 및 골이식술, 소파술 및 시멘트 충전술을 시행하였다. 후향적 연구 방법을 통해 골단과 견인골단에 발생한 연골 모세포종에서 발생 연령, 증상의 기간, 종양의 크기, 성장판의 개폐여부, 병적골절여부, 재발여부 및 동맥류성 골낭종의 동반여부 등의 차이를 분석하였다. 결과: 발생부위는 골단이 11례, 견인골단이 8례였다. 골단에 발생한 경우는 대퇴골 원위부가, 견인골단은 대퇴골 대전자가 가장 많았다. 평균 발생 연령은 골단이 14.2세, 견인골단이 22.3세로 통계적으로 유의한 차이를 보였다. 골단에 발생한 11례중 3례에서는 성장판 유합후에 발생하였으며, 견인골단에서는 전례(100%)에서 유합후에 발생하여 통계적으로 유의한 차이가 있었다. 종양의 크기는 견인골단에 발생한 경우 통계적으로 유의하게 큰 것으로 나타났다. 결론: 연골 모세포종은 주로 장관골의 골단에 호발하는 것으로 알려져 있으나 20세 이상에서는 견인골단에서 주로 발생하여 진단에 주의를 요한다. 두 그룹간 발생 연령, 성장판의 개폐여부 및 종양의 크기가 통계적으로 유의한 차이가 있었으며 증상의 기간, 병적골절, 재발, 동맥류성 골낭종의 동반여부에는 차이가 없는 것으로 나타났다. Purpose: Chondroblastoma is a rare benign bone tumor which occurs most frequently at epiphysis of long bones. This study analyzed the difference between patients with chondroblastoma either on their epiphysis or apophysis. Materials and Methods: We reviewed 19 patients with chondroblastoma who visited our hospital from August 1987 to August 2005. The mean follow up period was five years. Fifteen patients were male and 4 patients were female. The mean age of the patients was 17.6 years. The treatment consisted of either curettage alone, curettage with bone graft or curettage with cementation. We retrospectively compared the difference between one chondroblastoma originating from the epiphysis and the other chondroblastoma originating from the apophysis in terms of age predilection, duration of symptoms, size of tumor, status of the physis, presence of pathologic fracture, recurrence rate and accompanying aneurismal bone cyst. Results: Among the 19 patients, 11 patients had chondroblastoma at the epiphysis, and 8 at the apophysis. Distal femur was the most common site for epiphysis lesions while the greater trochanter was the most common site for lesions arising at the apophysis. The mean age was 14.2 years in the epiphysis group and 22.3 years in the apophysis group. Chondroblastoma occurred after closure of the physis in 3 out of 11 cases in the epiphysis group and in all 8 cases in the apophysis group showing a statistical significance between the groups in terms of status of the physis at onset. Size of the lesion was bigger in the apophysis group with statistical significance. Conclusion: Chondroblastoma is known to occur frequently at the epiphysis of long bones, but our study shows that in patients over 20 years old it occurs more frequently at the apophysis which needs to be considered when making the proper diagnosis. There was a significant difference between the two groups in terms of the age of occurrence, status of physis and size of tumor while there was none in terms of the duration of symptoms, presence of pathologic fracture, recurrence rate and presence of accompanying aneurismal bone cyst.

관절경적 미세골절술이 실패한 거골의 골연골 병변에 대한 치료로서의 재차 미세골절술

최우진,박광환,이모세,정광호,이진우,Choi, Woo Jin,Park, Kwang Hwan,Lee, Moses,Chung, Kwangho,Lee, Jin Woo 대한족부족관절학회 2015 대한족부족관절학회지 Vol.19 No.2

Arthroscopic treatment has been reported to provide effective improvement of ankle function when used in treatment of small osteochondral lesion of talus; however, favorable long-term results have been less predictable for large osteochondral lesion of talus. In cases in which primary arthroscopic treatment fails, the decision regarding which subsequent technique to choose has become increasingly difficult, as good clinical outcomes may be unlikely for such patients irrespective of the surgical technique used. Redomicrofracture should be used judiciously for treatment of osteochondral lesion of talus in which arthroscopic treatment has failed.