Pleiotrophin이 골수 줄기 세포의 부착 및 골형성에 미치는 효과에 대한 연구

윤정호,윤정주,장현석,임재석,이의석,김대성,권종진,Yoon, Jung-Ho,Eune, Jung-Ju,Jang, Hyon-Seok,Rim, Jae-Suk,Lee, Eui-Seok,Kim, Dae-Sung,Kwon, Jong-Jin 대한악안면성형재건외과학회 2006 Maxillofacial Plastic Reconstructive Surgery Vol.28 No.2

An area of current research is investigating the app1ication of human mesenchymal stem cells or hMSCs as a cell-based regenerative therapy. In order to achieve effective bone regeneration, appropriate matrices functioning as cell-carriers must be identified and optimized in terms of function, efficacy and biocompatibility. Two methods of approaching optimization of matrices are to facilitate adhesion of the donor hMSCs and furthermore to facilitate recruitment of host progenitor cells to osteoblastic differentiation. Pleiotrophin is an extracellular matrix protein that was first identified in developing rat brains and believed to be associated with developing neuronal pathways. A recent publication by Imai and colleagues demonstrated that transgenic mice with upregulated pleiotrophin expression developed a greater volume of cortical as well as cancellous bone. The proposed mechanism of action of pleiotrophin is demonstrated here. Through either environmental stresses and/or intracellular regulation, there is an increase in pleiotrophin production. The pleiotrophin is released extracellularly into areas requiring bone deposition. A receptor-mediated process recruits host osteoprogenitor cells into these areas. Therefore, the aim of our study was to investigate the osteoconductive properties of pleiotrophin. We wanted to determine if pleiotrophin coating facilitates cellular adhesion and furthermore if this has any effect on hMSCs derived bone formation in an animal model. The results showed a dose dependent response of cellular adhesion in fibronectin samples, and cellular adhesion was facilitated with increasing pleiotrophin concentrations. Histologic findings taken after 5 weeks implantation in SCID mouse showed no presence of bone formation with only a dense fibrous connective tissue. Possible explanations for the results of the osteogenesis assay include inappropriate cell loading.

HSP27 MODULATION OF IMPLANT- ASSOCIATED METAL ION CYTOTOXICITY OF OSTEOBLASTIC CELLS

윤정호,하동진,임재석,권종진,장현석,이의석,김대성,Yoon, Jung-Ho,Ha, Dong-Jin,Rim, Jae-Suk,Kwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Seok,Kim, Dae-Sung Korean Association of Maxillofacial Plastic and Re 2006 Maxillofacial Plastic Reconstructive Surgery Vol.28 No.2

Objectives: The extent of bone formation that occurs at the interface of metallic implants and bone is determined by the number and activity of osteoblastic cells. Stress proteins may be contributing determinants of cell viability in altered environments. Hsp27 is a small Mr hsp which is known as a molecular chaperone. Methods: To better understand how heat shock protein 27 contributes to endosseous implant - associated metal ions affects on osteoblastic cell viability, the effect of chromium and titanium ions were compared to effects of cadmium ions in the ROS17/2.8 osteoblastic cell line. Results: ROS17/2.8 osteoblastic cell line demonstrated ion - specific reductions in growth; reductions were significantly greater for cadmium than for chromium or titanium. Chromium impaired growth of cultures without altering cell viability measured using the MTT assay. A stable transformed cell line expressing additional hsp27(clone "A7") was resistant to the toxic effects of titanium and partially protected from cadmium toxicity. Conclusions: A role for hsp27 in protection of osteoblastic cells from metal ion toxicity is supported by the chromium - induced elevations in hsp27 abundance and the behavior of the A7 cell line in response to metal ions in culture. Similar biochemical responses to altered cellular environments may contribute to the fate of tissues adjacent to select metallic implants.

데이터 마이닝을 이용한 데이트코스 추천시스템

윤정호 ( Jung-ho Yoon ),김선호 ( Sun-ho Kim ),박두순 ( Doo-soon Park ) 한국정보처리학회 2013 한국정보처리학회 학술대회논문집 Vol.20 No.1

스마트폰이 급격하게 보급됨에 따라 스마트폰을 통해 다양한 정보를 편리하고 빠르게 수집하고 활용할 수 있다. 기존의 데이트코스 어플리케이션은 단순히 데이트코스를 검색하고 불특정 다수에게 추천을 해주기 때문에 개개인 취향과는 많이 다를 수가 있어서 사용자의 만족도가 떨어진다. 데이터 마이닝 기법 중 협업필터링 기법을 통해 사용자의 지역, 성별, 연령대등을 이용하여 개인에게 가장 잘 맞는 데이트코스를 추천하여준다. 따라서 본 논문에서는 스마트폰에서 데이트 코스 검색은 물론 데이터 마이닝 기법을 이용하여 사용자에게 만족도가 높은 데이트코스 추천을 통해 간편하고 만족도가 높은 데이트코스를 결정 할 수 있도록 도와준다.

스마트폰에서 데이터마이닝을 이용한 음식추천 시스템

윤정호 ( Jung-ho Yoon ),김선호 ( Sun-ho Kim ),박규태 ( Kyu-tae Park ),박두순 ( Doo-soon Park ) 한국정보처리학회 2012 한국정보처리학회 학술대회논문집 Vol.19 No.1

최근 스마트폰의 사용은 단순한 정보의 획득이 아닌 보다 높은 만족과 편의성을 얻을 수 있는 서비스가 각광을 받고 있다. 이러한 서비스 중에서 추천시스템은 고객이 좋아할 만한 서비스나 항목을 추천해주는 서비스이다. 본 논문에서는 이러한 추세에 맞추어서 안드로이드를 기반으로 사용자가 원하는 음식을 쉽게 검색도 하고, 데이터마이닝 기법을 이용하여 나이와 성별, 전날 먹은 음식의 종류에 따라 가중치를 부여하여 음식을 추천해주는 시스템을 개발하였다.

원형실린더를 이용한 Magnus 효과 추진 장치의 설계 및 제작

윤정호(Jung Ho Yoon),강원태(Won Tae Kang),김대호(Dae Ho Kim),박기현(Ki Hyun Park),신병록(Byeong Rog Shin) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11

Recently oil prices are rising day by day, and the costs of energies are extremely expensive. Because of this situation, in this project, one of the fundamental natural resources, wind power, is taken into account to make a driving force instead of generating the force with petroleum energies. Magnus effect caused by the rotating circular cylinder is applied to design and make a wind propulsion system The principle of Magnus effect is to get a driving force by pressure differences on the surface of rotating circular cylinder in the wind. As the results fluid dynamic force toward low pressure from high pressure is occurred. Through this project we could get more knowledge of fluid dynamics such as Bernoulli equation and the theory of Magnus effect. This knowledge and experience will be pretty useful in a design of devices working with a green energy in future and now.

Interferon Alfa - 2a로 치료하여 호전된 Kasabach - Merritt 증후군

윤정호(Jung Ho Yoon),김기호(Ki Ho Kim),조광열(Gwang Yeol Joh) 대한피부과학회 1995 대한피부과학회지 Vol.33 No.2

Kasabach- Merritt syndrame consists of thrombocytopenia, microangiopathic hemolytic anemia, and an acute or chronic censumptive coagulopathy in associati an with a rapidly enlarging hemangioma. Most vascular turrnrs causing Kasabach-Merritt syndon e are benign but the associated coagulopathy may be lifethreatening. Our patient, a 30-day-old female infant, was admitted after an increase in the size of a hemangioma present since birth on the right leg with extension to the lower abdomen and left inguinal area. Her herrnglobin count fell to 7.1g/dl, platelet count was 26,000/ul, prothrombin time was 26 seconds, and hypofibrinogenemia developed. The patient was treated with transfusions of platelets, fresh frozen plasma and red cells and prednisolone. for 2 weeks without improvement. We started interferon alfa-2a therapy. Over the past 6 months, he hemangioma progressively diminished in size withcut any serious complication. (Kor J Dermatol 1995;33(2): 363-368)

Toxic Pustuloderma

윤정호(Jung Ho Yoon),남종택(Jong Taik Nam),김기호(Ki Ho Kim),조광열(Gwang Yeol Joh) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.5

Generalized sterile pustular eruptions occur in various dermatoses including pustular psoriasis, erythema multiforme, Sneddon-Wilkinsan disease and others. Recentlr, acute eruptions of disseminated sterile pustules have been reported. The etiology is not related to a gepetic predisposition to psoriasis but to drug ingestion or viral infections, thus termed toxic pustuloderm; (T. P.). In this report, we present a typical case of T. P. observed iu our department. Our patient, an 18-year-old male, with no personal or family history of psoriasis, was given drug medication including amoxicillin for fever and chilling sensation. Pustilar eruptions first, appeared on his face about two days aft.er the medication and gradually spread to the trunk and limbs. The skin examination revealed numerous small pustules on an erythematous base. Laboratory examination revealed neutrophilic leukocytosis and an elevated sedimentation rate skin biopsy showed subeorneal and spongiform neutrophilic pustules Upon interruption of the amoxicillin, the pustules cleared rapidly in 3 days and there has been ri.o recui rence of any rash over a 7-month follow-up period. (Kor J Dermatol 1994;32(5):944-949)

피부 편평상피세포암 및 암전구증에서의 p53 단백질 및 Skin Sulfhydryl Oxidase의 발현

윤정호 ( Jung Ho Yoon ),김기호 ( Ki Ho Kim ),조광열 ( Gwang Yeol Joh ) 대한피부과학회 1996 大韓皮膚科學會誌 Vol.34 No.4

Background: Extensive study of the p53 gene has established its role as a tumor-suppressor gene, and the mutant p53 gene is expressed in a wide spectrum of human malignancy. The conversion of sulfhydryl groups to disulfide bonds in epidermis is carried out by skin sulfhydryl oxidase(SSO) at the junction of the granular layer and horny layers. So it is of interest to investigate the localization and properties of SSO in epidermal keratinocytic neoplasms. Objective: Our purpose is to investigate the expression pattern of the p53 protein and SSO and to gain a better understanding of biologic behavior in epidermal keratinocytic neoplasms. Methods : We investigated the expression pattern of p53 protein and SSO by immunohistochemical method on formalin-fixed, paraffin-embedded tissue specimens in 8 cases of squamous cell carcinomas, several cases of recancerosis(1 Bowen's disease, 1 actinic keratosis, 1 arsenic keratosis) and benign keratinizing disorders(1 verruca vulgaris, 1 condyloma accuminatum and 2 cases of lichen planus), Results: 1. Seven out of 8 cases of squamous cell carcinama(88%) and 1 case of 1 Bowen's disease showed p53 protein expression 2. SSO was weakly present in squamous cell carcinoma and precancerosis compared to the normal skin. In the well differentiated squamous cell carcinoma and Bowen's disease, the presence of SSO was rather diffuse than poorly differentiated squamous cell carcinoma and concentrated in dyskeratotic cells and horn pearls. 3. p53-stained cells or areas were seldom stained with SSO antibody, especially in invading borders of squamous cell carcinomas. Conclusion: Our results suggest that p53 mutation is a common genetic change in epidermal tumorigenesis and it can be useful marker of tumor invasion in the squamous cell carcinoma and precacerosis of skin. (Kor J Detmatol 1996;34(4): 616-621)

기업 관점의 인천신항 배후단지 입주결정 요인에 관한 연구

윤정호(Yoon Jung Ho),전준우(Jun Woo Jeon),여기태(Gi Tae Yeo) 한국항해항만학회 2016 한국항해항만학회지 Vol.40 No.3

본 연구는 항만배후단지 개발 및 임대방식의 변화를 겪고 있는 인천신항 항만배후지를 대상으로 하여, 기업 관점에서 고려하는 배후단지 입주결정요인을 도출하고, Fuzzy-AHP를 통해 입주결정 요인에 대한 우선순위를 분석하여 향후 인천신항 배후단지의 진입을 활성화하기 위한 방향을 제시하고자 하였다. 인천신항 배후단지 입주 결정요인들의 종합적인 가중치 순위를 평가하면 비용요인인 임대료 수준이 10.2%로 가장 높은 순위를 차지하였다. 다음으로 시장요인인 항만의 처리물동량이 8.2%, 배후시장의 규모가 7.3%, 비용요인인 내륙운송비 절감이 7.1%, 입지요인인 내륙교통 운송망과의 연계성이 6.7%, 자유무역지역의 지정 및 규모가 6.4% 순으로 나타났다. 인천신항 배후단지 입주를 결정할 기업측면에서 보면 임대료 수준을 타 지역 항만의 배후지에 비교하여 매력적으로 제공할 필요성이 있다. 또한 수도권 화주의 가장 큰 고민 사항인 내륙운송비를 절감하고, 인천항에서 미주 및 구주항로로 직접 연결할 수 있는 항로개설이 필요하다. The present study aimed to derive decision factors for residency in the hinterland of Incheon New Port that is undergoing the development of port hinterlands and changes in leasing methods considered from the perspective of companies and analyze the priorities of determinants for residency through Fuzzy-AHP in order to present a direction to activate companies entries into the hinterland of Incheon New Port. When the comprehensive rankings of determinants for residency in the hinterland of Incheon New Port, rent levels among cost factors took the highest ranking with a value of 10.2% followed by the throughput of the port among market factors with a value of 8.2%, the scale of the market on the background with a value of 7.3%,, reduction in inland transport costs among cost factors with a value of 7.1%, connectivity to inland transportation networks among locational factors with a value of 6.7%, and designation as a free trade zone and the scale with a value of 6.4%. When seen from the viewpoint of companies to determine whether to move into the hinterland of Incheon New Port , the rent level should be provided to be more attractive compared to the hinterlands of ports in other regions. In addition, inland transportation costs which are a matter of the most serious concern of shippers in the capital region should be reduced and sea routes that can directly connect Incheon New Port to US ports and European ports should be opened.

위성영상을 활용한 환경공간정보

윤정호(Yoon, Jeong Ho),이상혁(Lee, Sang Hyuk),이은정(Lee, Eun Jung),김재욱(Kim, Jaeuk),김유훈(Kim, Yu Hoon) 대한토목학회 2018 대한토목학회 학술대회 Vol.2018 No.10