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유지방구막 추출물로부터 얻은 인지질(Phosphatidylcholine, Sphingomyelin, Phosphatidylethanolamine) 분획물 제조에 관한 연구
이의석,빈국현,홍순택 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
본 연구는 유지방구막 추출물로부터 다량의 분획물 phosphatidylcholine(PC), sphingomyelin(SM)및 phosphatidylethanolamine(PE)의 제조에 관한 연구이다. 한외여과와 초임계 유체를 이용하여 버터밀크로부터 유지방구막 추출물을 제조하고 이 추출물에 에탄올 농도별(100, 60, 30%, 추출물:에탄올=1:20 비율)로 첨가하여 실온에서 1시간동안 추출하고 원심분리한 후 상등액과 하등액의 인지질 종류별 함량을 조사하였다. 그 결과, P의 경우 100% 에탄올 농도에서는 상등액에 대부분 잔류하였으나, 에탄올 농도가 낮아질수록 상등액에서 농도는 낮아지고 하등액에 잔류하였다. LPC는 100% 에탄올 농도에서 하등액에 대부분 잔류하였으나, 에탄올 농도가 낮아질수록 상등액에서 농도는 높아지고 하등액에서는 낮아졌다. SM 및 PE는 에탄올 농도에 관계없이 상등액과 하등액에 유사하게 분포하였다.
지방조직 유래 줄기세포의 조골세포로의 분화에 대한 실험적 연구
이의석,장현석,권종진,임재석,Lee, Eui-Seok,Jang, Hyon-Seok,Kwon, Jong-Jin,Rim, Jae-Suk 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.2
Stem cells have self-renewal capacity, long-term viability, and multiline age potential. Adult bone marrow contains mesenchymal stem cells. Bone marrow-derived mesenchymal stem cells (BMSCs) are progenitors of skeletal tissue components and can differentiate into adipocytes, chondrocytes, osteoblasts, and myoblasts in vitro and undergo differentiation in vivo. However, the clinical use of BMSCs has presented problems, including pain, morbidity, and low cell number upon harvest. Recent studies have identified a putative stem cell population within the adipose tissue. Human adipose tissue contains pluripotent stem cells simillar to bone marrow-derived stem cells that can differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic lineages. Human adipose tissue-derived stem cells (ATSCs) could be proposed as an alternative source of adult bone marrow stem cells, and could be obtained in large quantities, under local anesthesia, with minimal discomfort. Human adipose tissue obtained by liposuction was processed to obtain ATSCs. In this study, we compared the osteogenic differentiation of ATSCs in a specific osteogenic induction medium with that in a non-osteogenic medium. ATSCs were incubated in an osteogenic medium for 28 days to induce osteogenesis respectively. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific bone sialoprotein, osteocalcin, collagen type I and alkaline phosphatase, bone morphogenic protein 2, bone morphogenic protein 6 was confirmed by RT-PCR. ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. Since this cell population can be easily identified through fluorescence microscopy, it may be an ideal source of ATSCs for further experiments on stem cell biology and tissue engineering. The present results show that ADSCs have an ability to differentiate into osteoblasts. In the present study, we extend this approach to characterize adipose tissue-derived stem cells.
이의석,박동석,장현석,김현준,Besana,임호경 대한통합치과학회 2017 대한통합치과학회지 Vol.6 No.2
Lemierre’s syndrome is caused by preexisting oropharyngeal infection and characterized by internal jugular vein (IJV) thrombophlebitis. Frequently, this syndrome involves metastatic infection in areas such as the peritoneum, muscles, and spleen. The most common etiologic organism is Fusobacterium necrophorum. In this case, a masticatory space abscess that originated from a tooth caused the IJV thrombophlebitis. The patient had preexisting hypertension, diabetes mellitus, hyperlipidemia, and Parkinson’s disease. Blood culture revealed Streptococcus sanguinis, and the patient received antibiotics that were highly sensitive to mentioned bacterium; additionally, an anticoagulant was used. With the widespread use of antibiotics, Lemierre’s syndrome has become uncommon, and related mortality rates have noticeably decreased. This has led the public to forget about the disease, but it can still be potentially fatal without early diagnosis and appropriate antibiotic treatment.
이의석,김충남,김규태,임호경,김수호,김민수 대한통합치과학회 2018 대한통합치과학회지 Vol.7 No.2
The inferior alveolar nerve (IAN) is one of the branches of the mandibular division of the trigeminal nerve and innervates the mandible, mandibular teeth, gingiva of inferior alveolus, and lower lip. IAN dysfunction can occur during many dental surgical procedures and is associated with pathologic findings such as mandibular fractures, benign and malignant tumors, and osteomyelitis. We treated two patients with IAN injuries, caused by periapical and pericoronal lesions, by decompression via extraction and medication, and confirmed quantitative improvement. The aim of this article report was to review rare cases of successful treatment of IAN dysfunction following periapical and pericoronal lesions. 하치조신경은 삼차신경 중 하악분지이며, 하악, 하악치아, 하악치조골의 치은 및 하순의 감각을 지배한다. 하치조신경손상은 치과에서 시행하는 외과적 술식 중 일어날 수 있으며, 하악골 골절, 양성 및 악성종양, 골수염과 같은 병적소견과도 연관성이 있다. 본 병원에서는 치관주위염 및 치근단 병소로 유발된 감각이상에 대한 두 증례에서 발치술을 이용한 감압술 및 투약으로 성공적인 치료결과를 얻었고, 정량적인 방법으로서 호전양상을 평가했다. 따라서, 본 저자는 이처럼 드물게 발생하는 치관주위염 및 치근단 병소로 유발된 감각이상에 대한 두 증례를 보고하는 바이다.
치조골신장술과 자가골이식술의 안정성을 비교한 전향적 임상시험연구
이의석,이종현,최혁,윤태승,이학기,김현준,신재희 대한통합치과학회 2016 대한통합치과학회지 Vol.5 No.1
Introduction: The aim of this study was to compare the stability of using auto bone graft and distraction osteogenesis for vertical augmentation of resorbed alveolar ridges for implant installation. Method: A total of 14 patients with extremely resorbed alveolar bone were selected and randomly divided into two groups. Auto bone graft was used in the control group (n=7) and distraction osteogenesis was used for the experimental group (n=7). New bone formation was evaluated radiographically by comparing the differences in alveolar preoperatively, after 4 weeks, after 12 weeks and after 24 weeks. Results: Immediately after surgery (T1), an increase in vertical bone height of 4.12±1.01 mm was obtained in the control group. After 4 weeks (T2), vertical bone height was evaluated in each group (Control Group vs Experimental Group; 3.60±0.86 mm vs 5.77±1.71 mm). After 12 weeks (T3), the increase in vertical height of bone was about 3.18±0.68 mm (Control Group) vs 5.56±1.75 mm (Experimental Group). After 24 weeks (T4), increase in vertical bone height for the control group was 2.70±0.41 mm and 5.43±1.74 mm for the experimental group. Comparing bone resorption in each period, the bone resorption between 4 weeks after and 24 weeks after, showed significant difference between the two groups (p=0.03) (0.89±0.69 mm vs 0.34±0.29 mm). Conclusion: Distraction osteogenesis and auto bone graft can be used for reconstruction of horizontal and vertical alveolar bone defects with high prognosis when properly indicated. With 6 month observation period, distraction osteogenesis showed lower bone resorption, so it was a favorable procedure on alveolar bone augmentation.
이의석,주현중,장환용,이진용,임재석,장현석 대한통합치과학회 2014 대한통합치과학회지 Vol.3 No.2
Purpose: The purpose of this study was to compare histologic evaluation of autogenous and allogenous tooth material on bone-healing response in dog. Materials and Methods: Allogenous tooth biomaterial was grafted in the extraction socket of the premolar region in the left lower jaws and autogenous tooth biomaterial was grafted in the right side after 2 weeks. Three dogs were sacrificed after 4, 8, 12 weeks each for histological evaluation. Results: In the histologic analysis, there were no signigicant differences between group 1 (autogenous tooth bone biomaterial) and group 2 (allogenous tooth bone biomaterial) at 4, 8, 12 weeks. Statistical analysis showed that group 1 was more higher bone volume levels than group 2 at 4 weeks (P⁄0.05, Mann-whitney test). Group 2 showed more higher bone volume levels than group 1 at 8 weeks and group 1 more than group 2 at 12 weeks but that showed no significant differences (P¤0.05, Mann-whitney test). Conclusion: Histologically, autogenous and allogenous tooth biomaterial reveals similar healing patterns in extraction socket in dogs. The result of this study suggested that tooth biomaterials could be useful materials for socket preservation.