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Grape seed proanthocyanidin extract ameliorates monosodium iodoacetate-induced osteoarthritis
우윤주,조미라,주영빈,정영옥,주지현,오혜좌,전주연,박미경,박진실,강창민,성미숙,박성환,김호연,민준기 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.10
Osteoarthritis (OA) is an age-related joint disease that is characterized by degeneration of articular cartilage and chronic pain. Oxidative stress is considered one of the pathophysiological factors in the progression of OA. We investigated the effects of grape seed proanthocyanidin extract (GSPE), which is an antioxidant, on monosodium iodoacetate (MIA)-induced arthritis of the knee joint of rat, which is an animal model of human OA. GSPE (100 mg/kg or 300 mg/kg) or saline was given orally three times per week for 4 weeks after the MIA injection. Pain was measured using the paw withdrawal latency (PWL), the paw withdrawal threshold (PWT) and the hind limb weight bearing ability. Joint damage was assessed using histological and microscopic analysis and microcomputerized tomography. Matrix metalloproteinase-13 (MMP13) and nitrotyrosine were detected using immunohistochemistry. Administration of GSPE to the MIA-treated rats significantly increased the PWL and PWT and this resulted in recovery of hind paw weight distribution (P < 0.05). GSPE reduced the loss of chondrocytes and proteoglycan,the production of MMP13, nitrotyrosine and IL-1β and the formation of osteophytes, and it reduced the number of subchondral bone fractures in the MIA-treated rats. These results indicate that GSPE is antinociceptive and it is protective against joint damage in the MIA-treated rat model of OA. GSPE could open up novel avenues for the treatment of OA.
콜라겐유도관절염에서 Marginal Zone B 세포의 항체 생산능 증가
우윤주 ( Yun Ju Woo ),민준기 ( Jun Ki Min ),조미라 ( Mi La Cho ),김영주 ( Young Joo Kim ),전주연 ( Joo Yeon Jhun ),박민정 ( Min Jung Park ),문영미 ( Young Mi Moon ),박미경 ( Mi Kyung Park ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.1
Objective: Mature B cells in the spleen of mouse can be divide into two main subsets: the follicular (FO) B cells and the marginal zone (MZ) B cells. In this study, we investigated which subtype of B cells is involved in the production of costimulatory molecules, cytokine and antibody during the induction of autoimmune arthritis. Methods: The MZB and FOB cells isolated from DBA/1J induced- and collagen-induced arthritis (CIA) mice were stimulated with LPS or CpG. The costimulatory molecules were measured by flow cytometry (FACs). The cytokines were measured by ELISA. Production of antibodies by the MZB cells or FOB cells was measured by ELISA and the results were observed by confocal microscopy. Results: The expression of co-stimulatory molecules was stronger in the MZB cells than that in the FOB cells. The production of cytokines (IL-10, IL-6) and antibodies was higher in the MZB cells. The IgG expression of the MZB cells, which is known to be associated with the acceleration of autoimmunity, was higher in the CIA mice than that in the DBA/1J mice. Conclusion: We observed that the MZB cells were increased in the CIA mice. The costimulatory molecules, cytokine and auto-antibodies were increased in the MZB cells compared to that of the FOB cells. Our results suggest that MZB cells mainly produce autoantibodies, and they play a key role in development of autoimmune arthritis.
PD-L1 발현 B세포를 통한 활성 T세포의 IL-17 분비 조절
우윤주 ( Yun Ju Woo ),조미라 ( Mi La Cho ),민준기 ( Jun Ki Min ),주지현 ( Ji Hyeon Ju ),주세영 ( Se Young Ju ),박성환 ( Sung Hwan Park ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2008 대한류마티스학회지 Vol.15 No.2
목적: 최근 들어 면역조절능을 가지는 B세포의 존재에 관한 연구가 보고되고 있다. B세포의 특성 중 면역조절기능을 세포상호작용을 통해 연구하고자 본 실험에서는 B세포를 B세포 수용체(anti IgM)와 CD40에 대한 단클론항체(agonist anti mouse CD40 monoclonal antibody)로 자극하여 관찰하였다. 방법: 마우스의 비장에서 분리한 B세포를 lipopolysaccharide (LPS), B세포 수용체 또는 CD40에 대한 단클론항체로 자극하여 24시간 동안 배양하여 B세포에서 발현하는 공동신호분자(CD40, CD80, CD86, MHC-II, PD-1, PD-L1)를 유세포 분석기로 조사하였다. 그리고 활성화된 B세포를 anti CD3로 자극한 T세포와 공조 배양하여 T세포에서 발현하는 사이토카인을 중합효소연쇄반응(PCR, polymerase chain reaction)과 효소결합 면역흡수 분석법(ELSIA, enzyme-linked immunosorbent assay)으로 조사하였다. 결과: B세포에서의 공동신호분자는 자극(LPS, B세포 수용체 또는 CD40에 대한 단클론항체)에 대해 반응하여 모두 증가하는 경향을 나타냈으며 특히 B세포 수용체와 CD40에 대한 단클론항체로 자극하였을 경우 음성신호분자로 알려져 있는 PD-L1의 발현이 가장 의미 있게 증가되었다. B세포를 T세포와 공조배양 하면, T세포에서 분비되는 IL-17이 의미 있게 감소(nil: 1932±386 pg/mL vs anti IgM+agonist CD40mAb: 1193±334 pg/mL, p<0.01)하였다. 그러나 IFN-γ (nil: 4658±752 pg/mL, anti IgM+agonist CD40mAb: 5525±315 pg/mL)와 TNF-α (nil: 1445±178 pg/mL, anti IgM+agonist CD40mAb: 1,469±281 pg/mL)의 형성에는 변화가 없었다. 또한 공조배양을 통한 IL-17 생성 억제는 PD-L1 중성화 항체 처리로 회복되는 경향을 보였다. 결론: 이와 같은 연구로 B세포는 B세포 수용체와 CD40에 대한 단클론항체로 자극하면 PD-L1의 발현이 증가되고, T세포와 공조배양 시 IL-17분비를 뚜렷이 감소시킨다는 결과를 얻을 수 있었다. 이는 면역조절능을 가진 B세포는 PD-L1발현을 통해 결과적으로 IL-17의 생성을 억제시킨다는 것을 시사한다.
정지헌,우윤주,이미현,Muhammad R. Haque,안철희,이해신,변영로 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
Porcine islets are an alternative cell source for cell replacement therapy in diabetes. However, porcineislets are very fragile, and isolated islets are easily damaged during culture. Therefore, the purpose of thisstudy was to incorporate a tissue-adhesive chitosan derivative on the surface of porcine islets to preventthe dissociation of porcine islet clusters into single cells. Chitosan-catechol was evenly covered on thesurface of islets without any toxicity. In addition, the tissue-adhesive chitosan catechol was effective inpreventing cell dissociation. Therefore, tissue adhesive chitosan-catechol conjugation onto the porcineislet surface is a promising technique to stabilize islets after isolation.
A Simulation Study on a Workload-based Operation Planning Method in Container Terminals
정연호,김갑환,우윤주,서보현 대한산업공학회 2012 Industrial Engineeering & Management Systems Vol.11 No.1
A yard in a container terminal is a temporary storage space before containers are loaded onto the target vessel or delivered to consignees. For improving the utilization of the space in the yard and the efficiency of loading and discharging operations, it is important that operation plans must be carefully constructed in advance. A heuristic method is suggested to solve operation space planning problems considering workloads on handling equipment as well as space availabilities. The operation plans in this paper includes quay crane (QC) schedules and space plans for multiple vessels considering the workload in the container yard of container terminals. This paper evaluates the effectiveness of a space planning method and the performance of a new QC scheduling method using a simulation model.
류마티스관절염 활막세포에서 IL-17과 TLR 상호전달을 통한 RANKL과 IL-6 생성 조절
김경운 ( Kyoung Woon Kim ),이상헌 ( Sang Heon Lee ),조미라 ( Mi La Cho ),오혜좌 ( Hye Joa Oh ),우윤주 ( Yun Ju Woo ),김석형 ( Suk Hyung Kim ),김해림 ( Hae Rim Kim ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.1
Objective: The aim of this study was to clarify whether stimulation of recombinant IL-17, TLR2 and TLR4 by their specific ligands induces the production of RANKL and IL-6 in the fibroblast-like synoviocytes (FLSs) from RA patients. Methods: FLSs were isolated from RA synovial tissues and they were stimulated with the IL-17, TLR2 ligand bacterial peptidoglycan (PGN) and TLR4 ligand lipopolysaccharide (LPS). The RANKL levels were assessed by RT-PCR and western blotting. The expressions of IL-17, TLR2, TLR4, RANKL and IL-6 in the RA synovium were quantified by immunohistochemistry and these values were compared with the values obtained in the osteoarthritis synovium. The increased IL-6 production in the culture supernatants of the RA FLSs was quantified by sandwich ELISA. Results: The mRNA and protein levels of RANKL and IL-6 increased in the RA FLSs stimulated with PGN, LPS and IL-17, or PGN plus IL-17 or LPS plus IL-17. The expressions of IL-17, TLR2, TLR4, RANKL and IL-6 were much higher in the RA synovium than those in the osteoarthritis (OA) synovium. Conclusion: We observed synergistic effects of TLR-2, TLR-4 and IL-17 upon the induction of RANKL. In conclusion, our data supports the previous evidence of an important role of TLR-2, TLR-4 and IL-17 in the pathogenesis of RA.