日本と韓國の國際化と在日韓國 朝鮮人

王勝俊 동국대학교 일본학연구소 1999 일본학 Vol.18 No.-

흰쥐에서 염화비닐 폭로수준에 따른 소변내 thiodiglycolic acid 농도의 변화

왕승준,차봉석,노재훈,신동천,김명수,전근재 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : It is the objective of this research to identify the variation of thiodiglycolic acid(TdGA) in urine with vinyl chloride monamer(VCM) exposure levels through methylation. Methods : After rats were exposed to vinyl chloride monomer of 4 levels, 0㎎/㎥, 50㎎/㎥, 150㎎/㎥, 500㎎/㎥, respectively, of which urine was sampled in each sampling time of 0 hour, 2 hours, 4 hours, 8 hours, 16 hours, 36 hours, 48 hours. After urine had been methylated with diazomethane in the preliminary experiment and the peak of 146 m/z had been verified, the main experiment was done. Results : In the variation of TdGA with sampling times, concentration of TdGA increased rapidly in 4 hours and then decreased after 8 hours. When the variation of urinary TdGA concentration in urine according to exposure level of VGM was verified through Kruskal-Wallis statistical method at each sampling time, the significant increment with the exposure levels at 2 hours, 4 hours, 8 hours after exposure was clarified. Conclusion : TdGA concentration in urine with increment of VCM exposure level increased, especially significantly at 2 hours, 4 hours, 8 hours of sampling time.

Strategy of NIER for Research on The Elderly's Environmental Health

Seung-jun Wang(왕승준),Wook-Hee Choi(최욱희),So-Rin Oh(오소린),Chulwoo Lee(이철우),Seung-Do Yu(유승도) 한국환경독성학회 2010 한국독성학회 심포지움 및 학술발표회 Vol.2010 No.11

The Mutual Comparison of Test Methods(Guidelines) in the Area of Toxicokinetics

Seung-jun Wang(왕승준),Eun-hye Jo(조은혜),Eung-Ro Park(박응로),Chul-woo Lee(이철우),Hyung-hee Kim(김현미),Kyung-hee Choi(최경희),Hak-joo Kim(김학주) 환경독성보건학회 2008 한국독성학회 심포지움 및 학술발표회 Vol.- No.-

흰쥐에서 니켈 및 카드뮴의 동시노출이 간에 미치는 영향

차봉석,왕승준,장세진 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 이 연구는 적출간 관류법을 이용하여 영화니켈과 염화카드뮴의 동시 노출로 인하여 간에 나타나는 효과를 확인하는 데에 있다. 방법 : 간독성의 지표로서 AST, ALT, LDH의 농도 및 관류속도를 이용하였고 생존력에 대한 지표로서 산소소모율을 측정하였다. 300(±50) g의 흰쥐를 노출군(비노출군 및 50 μM/ 200 μM의 각 농도별 염화니켈과 염화카드뮴의 개별 투여군 및 동시 투여군)별로 5마리씩 총 35마리를 할당하였다. 간을 통과한 관류액을 채취하여 생화학적 간독성 지표를 측정했고 집단간의 시간의 경과에 따른 측정치의 비교를 위해 반복 측정된 2요인 분산분석을 실시하였다. 개별 투여군과 동시 투여군의 생화학적 지표간의 평균을 비교함으로써 상승효과에 대한 가능성을 확인했다. 결과 : 개별 투여군 및 동시 투여군 모두에서 AST, ALT, LDH가 증가했다. 관류속도 및 산소 소모율은 시간의 경과에 따라 감소했으나 통계적으로 유의한 차이를 보이지는 못했다. AST, ALT, LDH, 산소소모율이 일정 시간대 이후로 개별 노출군의 합보다 동시 노출군에서의 측정치가 더욱 크게 증가하였다. 결론 : 간독성에 대한 생화학적 지표의 변화를 확인함으로써 두 물질의 동시노출로 인한 복합적 효과는 상승효과일 가능성이 높은 것으로 보인다. Objectives : This research was intended to verify hepatic effect of simutaneously com-bined exposure of nickel chloride and cadmium chloride with IPRL (Isolated Perfumed Rat Liver) method. Methods : AST(aspartate aminotransferase), ALT(alanine aminotransferase), LDH (lac-tate dehydrogenase) and perfusion flow rate were used as the indicator of hepatotoxicity and oxygen consumption rate were used as the indicator of viability. 300(±50) g - weighted rats were allocated randomly to each group (control group, 50 μM·200 μM sera-rately exposure group of NiCl₂and CdCl₂, simultaneously combined exposure group of NiCl₂and CdCl₂by 5, totally 35. Buffer which got out of liver was sampled and then biochemical indicator of hepatotoxlcity was measured. In order to verify difference among groups, two way - repeated ANOVA was used. With comparing mean summation of sepa-rate exposure group with mean of simultaneous exposure group, possibility that combined effect could be synergistic effect was verified. Results : AST, ALT, LDH increased in both of separate exposure group and simultaneous exposure group. Perfusion flow rate and oxygen consumption rate decreased but statlstically significant difference among groups was not found. In each exposure group, AST, ALT, 7DH, oxygen consumption rate of simultaneous exposure group increased more than sum of separate exposure group after any sampling time. Conclusions : It was found that simultaneous combined effect could be synergistic effect through the biochemical indicator of hepatotoxicity.

국가경제침체가 근로자의 사회심리적 스트레스에 미치는 영향

장세진,왕승준,고상백,차봉석 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

This study was carried out to assess the effect of economic depression on psychosocial distress, and to assess the relationship between differences of psychosocial distress and job characteristics(job strain), and to find out the effect of social support on psychosocial distress. We measured job characteristics(job demand, job control), and social support(coworker support, supervisor support) at work by a self-administered questionnaire. Psycho social distress was measured by PWI(Psychosocial Well-being Index), a 45-item self-administered instrument. Among the 152 respondents, the prevalence of high job strain was 27%(41 persons). The Odds ratio of high job strain was 3.11(95% CI; 2.12∼4.11), and those of passive group and active group were 2.01(95% CI; 0.83∼2.76) and 1.80(95% CI; 0.83∼2.76), respectively. Our results supported the association between psychosocial distress and job strain. The odds ratio of high strain isolated group(high strain group+low social support) was 3.59(95% CI; 2.05?5.13), but the odds ratio of high strain collective group was 1.32(95% CI; 0.23?2.41). Social support at work was modifing factor of the association between psycho social distress and job strain. Therefore we reviewed the evidence that economic depression was associated with psycho social distress in this study, and that the strength of the social support was important in maintain health.

흰쥐에서 카드뮴과 니켈이 혈압에 미치는 효과

차봉석,왕승준,Cha, Bong-Suk,Wang, Seung-Jun 대한예방의학회 2001 Journal of Preventive Medicine and Public Health Vol.34 No.2

카드뮴과 니켈이 혈압에 미치는 영향과 더불어 복합적 노출이 혈압에 미치는 영향을 평가하고자, 250 g 정도의 흰쥐를 대조군과 노출군(두 농도의 카드뮴 노출군, 니켈 노출군, 복합노출군)에 각각 10마리씩 할당하고 염화카드뮴과 염화니켈을 생리식염수에 녹여 일주일간 매일 복강 내에 투여한 후 1, 5, 10, 20, 30일 경과 후 혈압의 변화를 관찰하였다. 각각 5회씩 혈압을 측정하였으며 최고치와 최저치를 제외한 3회 측정치의 평균값을 이용하여 각 시간대에서 각 물질간에 혈압의 차이가 있는지 확인하기 위하여 일요인 분산분석을 실시하였으며 시간을 반복측정요인으로 보고 반복측정된 이요인 분산분석을 실시하였다. 혈압의 변화에 있어서 0.1 mg/kg bw의 염화카드뮴 투여 후 1, 5, 10일 째에서 대조군과 통계적으로 유의한 차이를 보여 주었으며 같은 농도의 염화니켈은 전기간 동안 통계적으로 유의한 차이를 보여 주지 못했다. 이 농도에서 동시 노출시 대조군과는 1, 5, 10일째에서 통계적으로 유의한 차이(p<0.05)를 보여 주었으며 염화카드뮴 투여군과는 5일째에서, 염화니켈 투여군과는 5, 10일째에서 통계적으로 유의한 차이를 보여 주었다. 1.0 mg/kg bw의 염화카드뮴 투여 후 1, 5, 10, 20일째에서 대조군과 통계적으로 유의한 차이를 보여 주었으며 같은 농도에서 염화니켈의 경우, 1, 5일째에서 대조군과 통계적으로 유의한 차이를 보여 주었다. 이 농도에서 동시 노출시에는 대조군과 전 시간대에서 통계적으로 유의한 차이를 보여 주었으며 염화카드뮴 투여군과는 10, 20, 30일째에서, 니켈 투여군과는 5, 10, 20, 30일째에서 통계적으로 유의한 차이를 보였다. 결과적으로 카드뮴이 니켈에 비하여 혈압의 상승효과가 크며 복합 노출시 혈압의 상승이 오랫동안 지속되었다. Objective : To verify the separate and combined effects of cadmium and nickel on blood pressure in rats. Methods : Following the daily administration of cadmium chloride$(CdCl_2)$ and nickel chloride$(NiCl_2)$ to rats both individually and in combination with intraperitoneal injection method for one week, systolic blood pressure of the tail was measured at 1 day and 5, 10, 20, 30 days after administration. Each substance was injected into the rats with 0.1 mg/kg bw and 1.0 mg/kg bw concentration. Results : After 0.1 mg/kg bw $CdCl_2$ was injected, a statistically significant difference was found as compared with the control group(only saline) after 1, 5 and 10 days. After 0.1 mg/kg bw $NiCl_2$ was injected, a statistically significant difference was not found compared with the control group. After 0.1 mg/kg bw $CdCl_2$ and 0.1 mg/kg bw $NiCl_2$ were injected simultaneously, a statistically significant difference was found as compared with the control group after 1,5 and 10 days and compared with 0.1 mg/kg bw $CdCl_2$ group after 5 days and as compared with 0.1 mg/kg bw $NiCl_2$ group after 5 and 10 days. After 1.0 mg/kg bw $CdCl_2$ was injected, a statistically significant difference was found as compared with the control group after 1, 5, 10 and 20 days. After 1.0 mg/kg bw $NiCl_2$ was injected, a statistically significant difference was found as compared with the control group after 1 day and 5 days. After 1.0 mg/kg bw $CdCl_2$ and 1.0 mg/kg bw $NiCl_2$ were injected in combination, a statistically significant difference was found after 1, 5, 10, 20 and 30 days as compared with 1.0 mg/kg bw $CdCl_2$ after 10, 20 and 30 days and as compared with 1.0 mg/kg bw $NiCl_2$ after 5, 10, 20 and 30 days. Conclusion : It was found that the effect of $CdCl_2$ on blood pressure was much more than $NiCl_2$ and a high concentration $CdCl_2\;and\;NiCl_2$ in combination delayed the recovery of blood pressure.

야생조류의 조류인플루엔자 바이러스 분포와 이동경로 추적조사

신정화,왕승준,정집설,김용관,엄재구,정원화,모인필 한국가금학회 2016 한국가금학회 정기총회 및 학술발표회 Vol.2016 No.11

오비트랩 고분해능 질량분석기를 이용한 폐사 조류 중 다성분 잔류 농약 스크리닝 기법

이두희,김보경,왕승준,손기동,정현미,최종우 한국환경농학회 2017 한국환경농학회지 Vol.36 No.4

BACKGROUND: The objective of this study was to evaluate screening method of residual multi pesticides in dead birds by Orbitrap high resolution mass spectrometry (HRMS) to identify the cause of death for birds . METHODS AND RESULTS: Extraction and clean-up method of residual pesticides in liver of dead birdswas used QuEChERS (Quick Easy Cheap Effective Rugged and Safe) and method validations was conducted using liquid chromatography and gas chroamtography with triplequadrupole mass spectrometer (LC/MS/MS and GC/MS/ MS) Also, we were evaluated screening method for the determination of residual pesticides in liver of dead birds by LCandGCOrbitrapMass Spectrometry. Results ofmethod validations, Correlation coefficients of thematrixmatched calibration curves were >0.978, and the method detection limits (MDLs) and limits of quantitation (LOQ) were 2.8~ 72.1 ng/g (18.4 ng/g on average) and 9.0~230 ng/g (58.5 ng/g on average). The accuracy ranged from69.1%to 130% (103%on average), and the precision valueswere less than 14.8%(3.8%on average). The screening of residual pesticides in liver of dead birds by LC and GC Orbitrap HRMS was detectedmonocrotophos, carbofuran, carbosulfan, deltametrin, benfuracarb, carbofuran, phosphamidon, prochloraz in investigated samples. CONCLUSION: This results showed that accurate mass were extraction of residual pesticides in dead birds by Orbitrap HRMS. It suggested that this screeningmethod is applicable to the residual pesticide analysis for the cause of death as a main tool. .

Prevalence of avian influenza virus in wild birds before and after the HPAI H5N8 outbreak in 2014 in South Korea

신정화,우찬진,왕승준,Jipseol Jeong,안인정,황종경,조성덕,유승도,최경희,정현미,서재화,김설희 한국미생물학회 2015 The journal of microbiology Vol.53 No.7

Since 2003, highly pathogenic avian influenza (HPAI) virus outbreaks have occurred five times in Korea, with four HPAI H5N1 outbreaks and one HPAI H5N8 outbreak. Migratory birds have been suggested to be the first source of HPAI in Korea. Here, we surveyed migratory wild birds for the presence of AI and compared regional AI prevalence in wild birds from September 2012 to April 2014 for birds having migratory pathways in South Korea. Finally, we investigated the prevalence of AI in migratory birds before and after HPAI H5N8 outbreaks. Overall, we captured 1617 migratory wild birds, while 18,817 feces samples and 74 dead birds were collected from major wild bird habitats. A total of 21 HPAI viruses were isolated from dead birds, and 86 low pathogenic AI (LPAI) viruses were isolated from captured birds and from feces samples. Spatiotemporal distribution analysis revealed that AI viruses were spread southward until December, but tended to shift north after January, consistent with the movement of migratory birds in South Korea. Furthermore, we found that LPAI virus prevalences within wild birds were notably higher in 2013–2014 than the previous prevalence during the northward migration season. The data from our study demonstrate the importance of the surveillance of AI in wild birds. Future studies including in-depth genetic analysis in combination with evaluation of the movement and ecology of migratory birds might help us to bridge the gaps in our knowledge and better explain, predict, and ultimately prevent future HPAI outbreaks.