The Oxytocinergic Neurons in Hypothamo-hypophysial Tract Contributes to CNS Pathway Innervating Ovary in Rat

변경희,오지현,조승묵,이봉희,Byun, Kyung-Hee,Oh, Jee-Hyun,Jo, Seung-Mook,Lee, Bong-Hee Korean Society of Electron Microscopy 2010 Applied microscopy Vol.40 No.4

포유동물의 난소는 호르몬과 감각신경 및 교감신경의 지배를 받아 난소 내 혈류의 조절, 스테로이드 호르몬 생성 및 난포의 발달과 관계된 난소의 고유기능이 조절되고 있다. 본 실험에서는 통상적인 추적자인 CT-HRP와 Pseudorabies바이러스(PRV)의 Bartha strain을 신경추적자로 이용하여 뇌하수체 후엽 및 난소와 연결된 중추신경부위를 밝히고자 하였다. 또 oxytocin을 난소 지배 신경축속에서 동정함으로써 신경축내 oxytocinergic neuron들의 존재를 확인하고, 이들이 배란을 중심으로 한 난소생식주기에 따라 보이는 중추 내 oxytocin신경세포의 변화를 조사하고자 하였다. Sprague-Dawley 흰쥐를 대상으로 난소 내에 PRV를 주사하고 48시간 후 실험동물들은 4% paraformaldehyde-lysine periodae로 고정하였으며, 뇌를 적출하여 $30{\mu}m$ 두께의 관상연속 절편을 만들어 CTHRP, PRV 및 oxytocin에 대한 삼중염색을 시행하였다. 본 실험 결과 후뇌에서부터 전뇌에 이르기까지 PRV에 양성반응을 보인 신경핵들이 관찰되어 난소를 지배하는 신경축을 구성할 수 있었다. 또 시상하부의 뇌실옆핵에서 oxytocin, PRV와 CT-HRP에 삼중으로 염색된 세포가 관찰됨으로써 신경내분비축과 자율신경축이 공동으로 기원하고 있다는 것을 형태학적으로 보여주었다. 따라서 oxytocin은 이 두 계통 내에서 호르몬의 역할과 신경전달물질의 역할을 겸할 것이라는 것을 추측할 수 있었다. The mammalian ovary is innervated by sympathetic and sensory neurons which contribute to regulating several aspects of ovarian function, including blood flow, steroidogenesis and follicular development. The existence of a neural connection between central neurons and the ovary has been rarely reported, but the mechanism underlying integration of ovarian activity to broader neuroendocrine responses has not been reported. We have now used a viral transneuronal tracing technique combined with a conventional retrograde labeling procedure of CT-HRP to demonstrate that oxytocin-producing neurons of the hypothalamus are synaptically connected to the ovary. Since ovarian activity is suppressed but the activity of oxytocin neurons is increased during breast feeding. Our finding that the oxytocinergic neural connection is likely to provide a direct transsynaptic mechanism by which the central nervous system maintains the state of infertility that accompanies lactation in mammals.

초자화 동결법을 이용한 생쥐배아 발달단계별 동결성적의 비교

안기훈 ( Ki Hoon Ahn ),오지현 ( Jee Hyun Oh ),이정재 ( Jung Jae Lee ),김탁 ( Tak Kim ),김선행 ( Sun Haeng Kim ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.11

간호간병 통합서비스 간호업무에 대한 간호대학생의 인식

조미경,구병철,김가란,천홍섭,이지영,이환희,조아라,황가람,강신후,오지현,진현아,Cho, Mi-Kyoung,Koo, Byung-Chul,Kim, Ga-Ran,Cheon, Hong-Seop,Lee, Jee-Young,Lee, Hwan-Hee,Cho, A-Ra,Whang, Ga-Ram,Kang, Shin-Hu,Oh, Ji-Hyeon,Jin, Hyun-A 서울대학교 간호과학연구소 2017 간호학의 지평 Vol.14 No.2

Purpose: This study aims to identify the factors that affect the expected frequency of performance and the importance in nursing practice of total care service recognized by nursing college students. Methods: This is a descriptive study. A self-administered questionnaire survey was conducted on 288 nursing students of E university and data was collected and analyzed using the SPSS program. Results: The average scores for the expected frequency of performance and the importance in nursing practice of total care service were $4.07{\pm}0.58$ and $4.00{\pm}0.50$ respectively. The expected frequency of performance was found to have a significantly positive correlation with the importance in nursing practice. Additionally, the factors that influenced the degree of recognition in the expected frequency of performance and the importance in nursing practice of total care service among nursing students were comfortable care and respiratory care, respectively. Conclusion: The major finding of this study shows a significantly positive correlation between the expected frequency of performance and the importance in nursing practice. These results suggest that effective and systematic education is needed on factors affecting the expected frequency of performance and the importance in nursing practice for nursing college students.

완만동결법을 이용한 생쥐배아의 냉동 시 냉해보호제 Ethylene Glycol의 효용성

김연주 ( Yon Ju Kim ),김옥경 ( Ok Kyong Kim ),박은아 ( Eun Ah Park ),홍순철 ( Soon Cheol Hong ),유상욱 ( Sang Yook Yu ),김탁 ( Tak Kim ),이정재 ( Jung Jae Lee ),오지현 ( Jee Hyun Oh ),허준용,박용균,김선행 ( Sun Haeng Kim ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.7

목적: 포유류의 배아를 냉동하는 과정에서, 냉동 보호제와 냉동 방법은 해동 후 생존률과 그 이후의 배아 발달에 매우 중요한 인자이다. 이 실험에서 첫 번째로 완만 동결법에서 냉동 보호제인 ethylene glycol (EG)이 배아 발달에 어떤 영향을 끼치는지를 알아보고자 하였고, 두 번째로 각각의 배아 발달 시기에서 동결 방법에 따른 배아의 생존율 및 발달율 차이를 알아보고자 하였다. 연구 방법: 5-6주된 ICR생쥐로부터 1세포기 배아를 얻었다. 배아 배양을 위한 기본 배양액으로 0.4% BSA가 함유된 T6배지를 사용하였다. 1세포기, 8세포기, 포배기의 배아는 각각 EG, propylene glycol (PROH), glycerol을 사용하여 완만 동결법으로 냉동 보존하였다. 실험 후반부에서 동일한 냉동 보호제인 EG를 사용하여 완만 동결법과 초자화 동결법의 결과를 비교하였다. 끝으로 각 군별로 포배기 배아의 세포수를 측정하여 포배기의 질을 평가하였다. 결과: EG, PROH와 glycerol사이에 회수율, 생존율에서 유의할 만한 차이점은 발견할 수 없었다. 그러나 해동 후 배아 발달에 있어서는 EG군이 1세포기와 포배기 냉동 보존을 하였을 때 우수한 배아 발달을 보였다(P<0.05). 완만 동결법과 초자화 동결법의 비교에서 회수율은 두 그룹간에 차이를 보이지 않았으나, 초자화 그룹에서 유의하게 높은 생존율을 보여주었다(P<0.05). 해동 후 상실기와 포배기로의 발달에 있어서는 1세포기 냉동 보존시 초자화 동결법에서 좋은 발달율을 보였지만 (P<0.05) 8세포기와 포배기에서는 유의한 차이가 없었다. 포배기 배아의 세포수에 있어서 1세포기에 EG를 이용하여 냉동 보존된 군이 PROH, glycerol군에 비해 많은 것으로 나타났다 (P<0.05). 그러나 두 가지 냉동 방법에 따른 포배기 세포수의 차이는 없었다. 결론: 이상의 결과로 생쥐 배아 냉동시, EG가 지금까지 사용된 냉동 보호제인 PROH나 glycerol보다 우수한 것으로 나타났다. 냉동 방법은, 초자화 동결법이 완만 동결법보다 우수하였다. Objective: We intended to know how the cryoprotectant ethylene glycol (EG) would affect the outcome of the embryo development when used in slow freezing method. And to know if there is any difference in the outcome of frozen-thawed embryos according to freezing methods and the timing. Methods: We used 5-6 weeks old ICR female mice and T6 containing 0.4% BSA for basic culture media. The embryos at the developmental stages of 1-cell, 8-cell and blastocyst were cryopreserved respectively by slow freezing method using EG, propylene glycol (PROH), and glycerol as a cryoprotectant. We also compared the results of slow freezing and vitrification methods with the same cryoprotectant, EG. And finally, we evaluated the quality of blastocysts by counting the cell numbers in each group. Results: The post-thaw embryo development were better in EG group when they were frozen at 1-cell and blastocyst stage (P<0.05). Although there were no differences in the recovery rate, the survival rate in vitrification group was significantly higher (P<0.05). Post-thaw embryo development to morula and blastocyst were better in vitrification group when frozen at 1-cell embryo (P<0.05), not at 8-cell and blastocyst group. The cell counts of blastocyst derived from 1-cell stage frozen EG group were significantly increased than that of PROH-glycerol groups (P<0.05), however, there was no difference between the two freezing methods. Conclusion: These results suggest that EG may be advantageous comparing with the conventional cryoprotectants, PROH and glycerol in slow freezing method for mouse embryo cryopreservation. In terms of freezing method, vitrification is better than slow freezing.

안동 임청각의 공간구성에 관한 연구

오지현,김진균 대한건축학회 2000 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.20 No.2

Clonidine 을 첨가한 고비중 Bupivacaine 에 의한 척추마취효과

김영호,허금주,오지현,주진철 대한마취과학회 1997 Korean Journal of Anesthesiology Vol.33 No.2

Background : Vasoconstrictors have been used as an adjunct to local anesthetics to prolong the duration of spinal anesthesia. Recently, clonidine, an 2-receptor agonist has been shown to prolong the duration of spinal anesthesia following intrathecal administration. Bupivacaine has been used for spinal anesthesia and compared with tetracaine in recent studies. We have undertaken this study to further evaluate the effect of clonidine in hyperbaric 0.5% bupivacaine spinal anesthesia. Methods : Thirty patients who were scheduled for lower limb or urologic operation were divided into 2 groups: Group A(hyperbaric bupivacaine 13 mg, 2.6 ml+N/S 1 ml), Group B(hyperbaric bupivacaine 13 mg, 2.6 ml+clonidine 150 g, 1 ml). We used standardized techniques and injected above drugs to group A and B intrathecally for spinal anesthesia. We investigated the onset and the duration of spinal anesthesia along with hemodynamic changes(blood pressure and heart rate) in patients. Results : There were no significant differences the onset of spinal anesthesia and hemodynamic changes between two groups. The time taken to recover from the nerve block was more prolonged in the group B(touch 225, pain 262, foot dorsiflexion 271, knee flexion 290 minutes) than group A(touch 154, pain 188, foot dorsiflexion 198, knee flexion 216 minutes). There were no significant differences in sedation, and in experiencing dry mouth and other side effects between two groups. Conclusion : Intrathecal clonidine 150 g has been proved to prolong the duration of hyperbaric 0.5% bupivacaine spinal anesthesia without neurotoxicity or dangerous hemodynamic depression. Therefore, clonidine can be used as an effective adjunct in hyperbaric bupivacaine spinal anesthesia.(Korean J Anesthesiol 1997; 33: 304∼308)