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선천성 근긴장성 이영양증의 임상진단 및 분자생물학적 진단 1 예
김연주(Yon Ju Kim),김문영(Moon Young Kim),이봄이(Bom Yi Lee),김진우(Jin Woo Kim),박소연(So Yeon Park),김지은(Ji Eun Kim),오동출(Dong Chul Oh),한혜경(Hae Kyoung Han),김미정(Mi Jung Kim),전이경(Yi Kyeong Chun),김혜선(Hye Sun Kim),류현미(H 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.12
N/A Congenital myotonic dystrophy is a severe and early-onset form of myotonic dystrophy (DM) with a prevalence of 2.5-5.5/100,000 live births. Expansion of the trinucleotide CTG repeat in the 3´ untranslated region of the DM gene, which is located at a chromosome 19q13.3 is a common mutation in DM. Clinical features are generalized hypotonia (floppy infant), respiratory and feeding difficulty, and the neonatal mortality rate is approximately 40%. We experienced a case of recurrent congenital myotonic dystrophy, and report with a review of related literatures. Women with recurrent neonatal hypotonia or ultrasonographic evidence of hypotonia, including positional abnormalities of the extremities and idiopathic polyhydramnios, should be offered testing for the genetic studies for myotonic mutation, such as PCR (Polymerase chain reaction) analysis and Southern blot analysis.
완만동결법을 이용한 생쥐배아의 냉동 시 냉해보호제 Ethylene Glycol의 효용성
김연주 ( Yon Ju Kim ),김옥경 ( Ok Kyong Kim ),박은아 ( Eun Ah Park ),홍순철 ( Soon Cheol Hong ),유상욱 ( Sang Yook Yu ),김탁 ( Tak Kim ),이정재 ( Jung Jae Lee ),오지현 ( Jee Hyun Oh ),허준용,박용균,김선행 ( Sun Haeng Kim ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.7
목적: 포유류의 배아를 냉동하는 과정에서, 냉동 보호제와 냉동 방법은 해동 후 생존률과 그 이후의 배아 발달에 매우 중요한 인자이다. 이 실험에서 첫 번째로 완만 동결법에서 냉동 보호제인 ethylene glycol (EG)이 배아 발달에 어떤 영향을 끼치는지를 알아보고자 하였고, 두 번째로 각각의 배아 발달 시기에서 동결 방법에 따른 배아의 생존율 및 발달율 차이를 알아보고자 하였다. 연구 방법: 5-6주된 ICR생쥐로부터 1세포기 배아를 얻었다. 배아 배양을 위한 기본 배양액으로 0.4% BSA가 함유된 T6배지를 사용하였다. 1세포기, 8세포기, 포배기의 배아는 각각 EG, propylene glycol (PROH), glycerol을 사용하여 완만 동결법으로 냉동 보존하였다. 실험 후반부에서 동일한 냉동 보호제인 EG를 사용하여 완만 동결법과 초자화 동결법의 결과를 비교하였다. 끝으로 각 군별로 포배기 배아의 세포수를 측정하여 포배기의 질을 평가하였다. 결과: EG, PROH와 glycerol사이에 회수율, 생존율에서 유의할 만한 차이점은 발견할 수 없었다. 그러나 해동 후 배아 발달에 있어서는 EG군이 1세포기와 포배기 냉동 보존을 하였을 때 우수한 배아 발달을 보였다(P<0.05). 완만 동결법과 초자화 동결법의 비교에서 회수율은 두 그룹간에 차이를 보이지 않았으나, 초자화 그룹에서 유의하게 높은 생존율을 보여주었다(P<0.05). 해동 후 상실기와 포배기로의 발달에 있어서는 1세포기 냉동 보존시 초자화 동결법에서 좋은 발달율을 보였지만 (P<0.05) 8세포기와 포배기에서는 유의한 차이가 없었다. 포배기 배아의 세포수에 있어서 1세포기에 EG를 이용하여 냉동 보존된 군이 PROH, glycerol군에 비해 많은 것으로 나타났다 (P<0.05). 그러나 두 가지 냉동 방법에 따른 포배기 세포수의 차이는 없었다. 결론: 이상의 결과로 생쥐 배아 냉동시, EG가 지금까지 사용된 냉동 보호제인 PROH나 glycerol보다 우수한 것으로 나타났다. 냉동 방법은, 초자화 동결법이 완만 동결법보다 우수하였다. Objective: We intended to know how the cryoprotectant ethylene glycol (EG) would affect the outcome of the embryo development when used in slow freezing method. And to know if there is any difference in the outcome of frozen-thawed embryos according to freezing methods and the timing. Methods: We used 5-6 weeks old ICR female mice and T6 containing 0.4% BSA for basic culture media. The embryos at the developmental stages of 1-cell, 8-cell and blastocyst were cryopreserved respectively by slow freezing method using EG, propylene glycol (PROH), and glycerol as a cryoprotectant. We also compared the results of slow freezing and vitrification methods with the same cryoprotectant, EG. And finally, we evaluated the quality of blastocysts by counting the cell numbers in each group. Results: The post-thaw embryo development were better in EG group when they were frozen at 1-cell and blastocyst stage (P<0.05). Although there were no differences in the recovery rate, the survival rate in vitrification group was significantly higher (P<0.05). Post-thaw embryo development to morula and blastocyst were better in vitrification group when frozen at 1-cell embryo (P<0.05), not at 8-cell and blastocyst group. The cell counts of blastocyst derived from 1-cell stage frozen EG group were significantly increased than that of PROH-glycerol groups (P<0.05), however, there was no difference between the two freezing methods. Conclusion: These results suggest that EG may be advantageous comparing with the conventional cryoprotectants, PROH and glycerol in slow freezing method for mouse embryo cryopreservation. In terms of freezing method, vitrification is better than slow freezing.
임신 중절 적응증의 최근 경향 변화 ( 1993 - 2000 )
김지은(Ji Eun Kim),한정열(Jung Yeol Han),오동출(Dong Chul Oh),김연주(Yon Ju Kim),정영철(Young Chul Chung),정상희(Sang Hee Jung),최준식(June Seek Choi),박소연(So Yeon Park),안현경(Hyun Kyong Ahn),오민정(Min Jeong Oh),류현미(Hyun Mee R 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.11
N/A Objective : To evaluate the recent trend of indication for pregnancy termination. Method : From 1993 to 2000, 1,087 cases of termination out of 61,842 cases of deliveries in Samsung cheil hospital were analyzed. We reviewed retrospectively the data-base and charts of delivery, and analyzed the indication for pregnancy termination. Results : Among 61,842 cases of deliveries, indications of pregnancy termination were fetal structure anomalies in 399 cases (0.7%), IUFD in 261 cases (0.4%), PROM in 215 cases (0.4%), chromosomal anomalies in 138 cases (0.2%), anhydroamnios in 32 cases (0.05%), rubella infection of mother or fetus in 24 cases (0.04%), and others in 20 cases (0.03%). Autopsy was performed in 242 cases of fetal anomalies (60.7%), 116 cases of UIFD (44%), and 59 cases of fetal chromosomal abnormalities (43%). The cases of chromosomal anomaly as indication of termination increased and rubella infection of mother or fetus decreased recently, and it is statistically significant r=0.95(P=0.00) and r=-0.73(P=0.04). The fetal weight terminated due to PROM is significantly decreased (Y=517-26 x year P=0.002). Conclusion : The indications of termination for fetal chromosomal abnormalities were increased, but for rubella infections were decreased. In cases of PROM, the terminated fetal weight were significantly decreased. However, no change was observed in cases of fetal anomaly, IUFD,and PROM.
자궁내막증에 의한 골반 통증을 호소하는 불임환자에서 골반경하 자궁천골인대 소작술 ( laparoscopic uterosacral nerve ablation ) 의 임상적 효용성
문명진(Myoung Jin Moon),김대곤(Dae Kon Kim),윤경호(Kyung Ho Yoon),홍준식(Jun Sig Hong),김연주(Yon Ju Kim),김혜옥(Hye Ok Kim),양광문(Kwang Moon Yang),최규홍(Kyu Hong Choi),유근재(Keun Jai Yoo) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.12
N/A Objective : The aim of this study was to evaluate the efficacy of laparoscopic uterosacral nerve ablation (LUNA) in infertile women with chronic pelvic pain and endometriosis. Method : Prospective randomized study was performed in 20 infertile women with endometriosis and chronic pelvic pain, who had undergone of laparoscopic uterosacral nerve ablation at Samsung Cheil hospital between April and September, 1998. Statistical analysis was perfomed using by DBSTAT (ver. 2.0). Result : The pelvic pain score of pre-operation was 3.72±1.07 (mean±SD) and it was significantly decreased to 1.83±0.85 at 1 month later and 1.93±0.92, 1.69±0.60, 1.56±0.62, 1.56±0.81 each in second, third, fourth and fifth month after operation. There was no correlation between pelvic pain and stage of endometriosis. Pregnancy rate was 65% after LUNA (twelve deliveries, one pregnancy is ongoing at IUP 24 weeks). Conclusion : Laparoscopic uterosacral nerve ablation would be effective for infertile women with endometriosis and pelvic pain.
최준식 ( June Seek Choi ),양재혁 ( Jae Hyug Yang ),류현미 ( Hyun Mee Ryu ),정상희 ( Sang Hee Jung ),문명진 ( Myoung Jin Moon ),김연주 ( Yon Ju Kim ),정진훈 ( Jin Hoon Chung ),최규홍 ( Kyu Hong Choi ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.1
목적 : 임신 제 1 삼분기 및 제 2 삼분기에 시행하는 산전 초음파 검사가 보편화 됨에 따라 부수적으로 발견되는 난소 종양이 증가하고 있다. 따라서 난소 종양 양상, 임신 중 처치 방법, 수술 시기 및 임신 경과에 미치는 영향을 알아보고자 하였다. 연구 방법 : 1996년 1월부터 2001년 12월까지 삼성제일병원 산부인과에서 산전 검사 및 분만을 시행한 50,126명 중 임신 중 난소 종양으로 진단받은 255명의 임산부를 대상으로 하였다. 대상 임산 Objective : To evaluate patients with adnexal masses that were managed surgically during pregnancy and their effect on fetal outcome. Methods : The data were reviewed concerning pregnant women who required surgery at Samsung Cheil hospital between January